Published in Eur J Biochem on September 01, 1986
Type II restriction endonucleases--a historical perspective and more. Nucleic Acids Res (2014) 1.22
Synthesis and application of derivatizable oligonucleotides. Nucleic Acids Res (1987) 1.14
Oligodeoxynucleotides containing 4-thiothymidine and 6-thiodeoxyguanosine as affinity labels for the Eco RV restriction endonuclease and modification methylase. Nucleic Acids Res (1992) 1.14
Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI. Nucleic Acids Res (1986) 1.10
An RNA-protein contact determined by 5-bromouridine substitution, photocrosslinking and sequencing. Nucleic Acids Res (1994) 1.07
Crosslinking transcription factors to their recognition sequences with PtII complexes. Nucleic Acids Res (1992) 1.04
A new affinity reagent for the site-specific, covalent attachment of DNA to active-site nucleophiles: application to the EcoRI and RsrI restriction and modification enzymes. Nucleic Acids Res (1992) 1.00
Purification and properties of the MboII, a class-IIS restriction endonuclease. Nucleic Acids Res (1992) 0.99
Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence. Nucleic Acids Res (1995) 0.95
Determination of the DNA-interacting region of the archaebacterial chromosomal protein MC1. Photocrosslinks with 5-bromouracil-substituted DNA. Nucleic Acids Res (1991) 0.85
DNA duplexes with reactive dialdehyde groups as novel reagents for cross-linking to restriction- modification enzymes. Nucleic Acids Res (1997) 0.79
Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site. Biochemistry (1984) 3.03
A monoclonal antibody to the heavy chain of clathrin. EMBO J (1983) 1.94
Horizontal gene transfer contributes to the wide distribution and evolution of type II restriction-modification systems. J Mol Evol (1996) 1.82
Acute severe spinal cord dysfunction in bacterial meningitis in adults: MRI findings suggest extensive myelitis. Arch Neurol (2001) 1.79
The interaction of the EcoRI restriction endonuclease with its substrate. A physico-chemical study employing natural and synthetic oligonucleotides and polynucleotides. Eur J Biochem (1980) 1.75
Cerebellar leukoencephalopathy: most likely histiocytosis-related. Neurology (2008) 1.73
Influence of DNA target melting behavior on real-time PCR quantification. Clin Chem (2000) 1.66
A site-directed mutagenesis study to identify amino acid residues involved in the catalytic function of the restriction endonuclease EcoRV. Biochemistry (1992) 1.62
Linear diffusion of restriction endonucleases on DNA. J Biol Chem (1985) 1.54
Ternary complex formation between elongation factor Tu, GTP and aminoacyl-tRNA: an equilibrium study. Eur J Biochem (1977) 1.50
Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg. Proc Natl Acad Sci U S A (1989) 1.48
Pausing of the restriction endonuclease EcoRI during linear diffusion on DNA. Biochemistry (1994) 1.46
A simple algorithm for detecting circular permutations in proteins. Bioinformatics (1999) 1.39
Kinetic characterization of linear diffusion of the restriction endonuclease EcoRV on DNA. Biochemistry (1998) 1.39
Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases. Nucleic Acids Res (1986) 1.34
Linear diffusion of the restriction endonuclease EcoRV on DNA is essential for the in vivo function of the enzyme. EMBO J (1996) 1.34
The stereochemical course of the restriction endonuclease EcoRI-catalyzed reaction. J Biol Chem (1984) 1.33
Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis. Biochemistry (1991) 1.32
Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes. Proc Natl Acad Sci U S A (1993) 1.31
Accuracy of the EcoRI restriction endonuclease: binding and cleavage studies with oligodeoxynucleotide substrates containing degenerate recognition sequences. Biochemistry (1990) 1.31
The influence of sequences adjacent to the recognition site on the cleavage of oligodeoxynucleotides by the EcoRI endonuclease. Eur J Biochem (1984) 1.30
Equivalent and non-equivalent binding sites for tRNA on aminoacyl-tRNA synthetases. Eur J Biochem (1975) 1.29
Mg2+ confers DNA binding specificity to the EcoRV restriction endonuclease. Biochemistry (1992) 1.27
Inhibition of Eco RI action by polynucleotides. A characterization of the non-specific binding of the enzyme to DNA. Nucleic Acids Res (1980) 1.25
Complaints by patients. BMJ (1993) 1.21
Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques. Anal Biochem (1991) 1.20
Topological similarities in TGF-beta 2, PDGF-BB and NGF define a superfamily of polypeptide growth factors. Structure (1993) 1.20
Evidence for an evolutionary relationship among type-II restriction endonucleases. Gene (1995) 1.19
Sau3AI, a monomeric type II restriction endonuclease that dimerizes on the DNA and thereby induces DNA loops. J Biol Chem (2001) 1.18
A fast and accurate enzyme-linked immunosorbent assay for the determination of the DNA cleavage activity of restriction endonucleases. Anal Biochem (1993) 1.16
The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate. EMBO J (1999) 1.15
A similar active site for non-specific and specific endonucleases. Nat Struct Biol (1999) 1.15
A comparison of the structural requirements for DNA cleavage by the isoschizomers HaeIII, BspRI and BsuRI. Eur J Biochem (1985) 1.14
Naturally occurring circular permutations in proteins. Protein Eng (2001) 1.14
On the catalytic mechanism of EcoRI and EcoRV. A detailed proposal based on biochemical results, structural data and molecular modelling. FEBS Lett (1992) 1.14
Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis. Nucleic Acids Res (1996) 1.13
Distinct steps in the specific binding of tRNA to aminoacyl-tRNA synthetase. Temperature-jump studies on the serine-specific system from yeast and the tyrosine-specific system from Escherichia coli. Eur J Biochem (1976) 1.11
Introduction of asymmetry in the naturally symmetric restriction endonuclease EcoRV to investigate intersubunit communication in the homodimeric protein. Proc Natl Acad Sci U S A (1996) 1.10
Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI. Nucleic Acids Res (1986) 1.10
The elongation factor Tu binds aminoacyl-tRNA in the presence of GDP. J Biol Chem (1982) 1.09
Transient cleavage kinetics of the Eco RI restriction endonuclease measured in a pulsed quench-flow apparatus: enzyme concentration-dependent activity change. Nucleic Acids Res (1981) 1.09
Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides. Nucleic Acids Res (1988) 1.08
Accuracy of the EcoRV restriction endonuclease: binding and cleavage studies with oligodeoxynucleotide substrates containing degenerate recognition sequences. Biochemistry (1995) 1.07
On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target. Protein Eng (2000) 1.06
The effect of several nucleic acid binding drugs on the cleavage of d(GGAATTCC) and pBR 322 by the Eco RI restriction endonuclease. Nucleic Acids Res (1981) 1.05
Cystic fibrosis with three mutations in the cystic fibrosis transmembrane conductance regulator gene. Hum Genet (1991) 1.03
Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-SceI. Nucleic Acids Res (1996) 1.03
Probing the indirect readout of the restriction enzyme EcoRV. Mutational analysis of contacts to the DNA backbone. J Biol Chem (1996) 1.03
DNA binding specificity of the EcoRV restriction endonuclease is increased by Mg2+ binding to a metal ion binding site distinct from the catalytic center of the enzyme. Biochemistry (1995) 1.03
TNF-α respecifies human mesenchymal stem cells to a neural fate and promotes migration toward experimental glioma. Cell Death Differ (2010) 1.02
Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye. Anal Biochem (1991) 1.01
Amino acid substitution (Ile194----Thr) in exon 5 of the lipoprotein lipase gene causes lipoprotein lipase deficiency in three unrelated probands. Support for a multicentric origin. J Clin Invest (1991) 1.01
Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases. Biochemistry (1984) 1.00
Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis. Nucleic Acids Res (1994) 0.99
Does the restriction endonuclease EcoRV employ a two-metal-Ion mechanism for DNA cleavage? Biochemistry (1997) 0.99
Aminoacyl transfer ribonucleic acid binding site of the bacterial elongation factor Tu. Biochemistry (1980) 0.98
Polymerase chain reaction detection of a highly polymorphic VNTR segment in intron 1 of the human p53 gene. Clin Chem (1993) 0.98
Identification of the protein product of the c-mos proto-oncogene in mouse testes. Oncogene (1988) 0.98
Aspects of the mechanism of action of local anesthetics on the sarcoplasmic reticulum of skeletal muscle. Biochim Biophys Acta (1976) 0.97
Spermidine increases the accuracy of type II restriction endonucleases. Suppression of cleavage at degenerate, non-symmetrical sites. Eur J Biochem (1985) 0.97
Sequence preferences in cleavage of dsDNA and ssDNA by the extracellular Serratia marcescens endonuclease. Biochemistry (1995) 0.95
A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. Protein Expr Purif (1994) 0.95
The recognition of methylated DNA by the GTP-dependent restriction endonuclease McrBC resides in the N-terminal domain of McrB. Biol Chem (1997) 0.95
Engineering novel restriction endonucleases: principles and applications. Trends Biotechnol (1996) 0.94
Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease. Eur J Biochem (1996) 0.94
The role of translocation in ribosomal accuracy. Translocation rates for cognate and noncognate aminoacyl- and peptidyl-tRNAs on Escherichia coli ribosomes. J Biol Chem (1987) 0.94
Does the specific recognition of DNA by the restriction endonuclease EcoRI involve a linear diffusion step? Investigation of the processivity of the EcoRI endonuclease. Nucleic Acids Res (1983) 0.93
Fluorescence stopped-flow kinetics of the cleavage of synthetic oligodeoxynucleotides by the EcoRI restriction endonuclease. Biochemistry (1989) 0.93
Biochemical characterization of Anabaena sp. strain PCC 7120 non-specific nuclease NucA and its inhibitor NuiA. Eur J Biochem (1998) 0.93
Calcium gradient-dependent and calcium gradient-independent phosphorylation of sarcoplasmic reticulum by orthophosphate. The role of magnesium. Eur J Biochem (1978) 0.93
Semiautomated quantitative detection of loss of heterozygosity in the tumor suppressor gene p53. Biotechniques (1995) 0.92
Identification of Asp218 and Asp326 as the principal Mg2+ binding ligands of the homing endonuclease PI-SceI. Biochemistry (2000) 0.92
Two identical subunits of the EcoRI restriction endonuclease Co-operate in the binding and cleavage of the palindromic substrate. Eur J Biochem (1982) 0.92
Characterization of the interaction between the restriction endonuclease McrBC from E. coli and its cofactor GTP. J Mol Biol (1997) 0.92
On the advantage of being a dimer, a case study using the dimeric Serratia nuclease and the monomeric nuclease from Anabaena sp. strain PCC 7120. J Biol Chem (1999) 0.91
The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding. Nucleic Acids Res (1998) 0.91
Protein engineering of the restriction endonuclease EcoRV: replacement of an amino acid residue in the DNA binding site leads to an altered selectivity towards unmodified and modified substrates. Biochim Biophys Acta (1994) 0.91
The production and characterization of artificial heterodimers of the restriction endonuclease EcoRV. Biol Chem (1996) 0.91
Comparative investigations of algorithms for the detection of breaths in newborns with disturbed respiratory signals. Comput Biomed Res (1998) 0.90