Published in Genes Dev on October 01, 2000
Mapping determinants of human gene expression by regional and genome-wide association. Nature (2005) 17.24
c-Myc regulates transcriptional pause release. Cell (2010) 11.64
Methylation of histone H3 by Set2 in Saccharomyces cerevisiae is linked to transcriptional elongation by RNA polymerase II. Mol Cell Biol (2003) 6.79
Progression through the RNA polymerase II CTD cycle. Mol Cell (2009) 5.62
RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. Mol Cell Biol (2002) 5.04
Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast. Genes Dev (2003) 4.93
Nuclear CDKs drive Smad transcriptional activation and turnover in BMP and TGF-beta pathways. Cell (2009) 4.81
Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain. Genes Dev (2001) 4.64
Gene-specific requirement for P-TEFb activity and RNA polymerase II phosphorylation within the p53 transcriptional program. Genes Dev (2006) 4.06
Evidence for eviction and rapid deposition of histones upon transcriptional elongation by RNA polymerase II. Mol Cell Biol (2004) 4.06
The splicing factor SC35 has an active role in transcriptional elongation. Nat Struct Mol Biol (2008) 3.86
Dimethylation of histone H3 at lysine 36 demarcates regulatory and nonregulatory chromatin genome-wide. Mol Cell Biol (2005) 3.83
Dynamic histone H3 methylation during gene induction: HYPB/Setd2 mediates all H3K36 trimethylation. EMBO J (2007) 3.81
A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation. Mol Cell Biol (2005) 3.79
Transcription factor and polymerase recruitment, modification, and movement on dhsp70 in vivo in the minutes following heat shock. Mol Cell Biol (2003) 3.65
NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes Dev (2003) 3.65
The S. cerevisiae SAGA complex functions in vivo as a coactivator for transcriptional activation by Gal4. Genes Dev (2001) 3.54
Transitions in RNA polymerase II elongation complexes at the 3' ends of genes. EMBO J (2004) 3.50
The Yin and Yang of P-TEFb regulation: implications for human immunodeficiency virus gene expression and global control of cell growth and differentiation. Microbiol Mol Biol Rev (2006) 3.44
RNA polymerase II pauses and associates with pre-mRNA processing factors at both ends of genes. Nat Struct Mol Biol (2007) 3.36
"Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions. Mol Cell (2009) 3.28
Chromatin remodeling protein Chd1 interacts with transcription elongation factors and localizes to transcribed genes. EMBO J (2003) 3.26
Predictive chromatin signatures in the mammalian genome. Hum Mol Genet (2009) 3.25
Uniform transitions of the general RNA polymerase II transcription complex. Nat Struct Mol Biol (2010) 3.25
Stable mRNP formation and export require cotranscriptional recruitment of the mRNA export factors Yra1p and Sub2p by Hpr1p. Mol Cell Biol (2002) 3.18
Histone H2A monoubiquitination represses transcription by inhibiting RNA polymerase II transcriptional elongation. Mol Cell (2008) 3.18
The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. Nat Struct Mol Biol (2008) 3.17
RNA polymerase II elongation control. Annu Rev Biochem (2012) 3.09
The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo. Mol Cell Biol (2003) 3.03
Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol (2003) 2.77
Capping, splicing, and 3' processing are independently stimulated by RNA polymerase II: different functions for different segments of the CTD. Genes Dev (2001) 2.72
CDK8 is a positive regulator of transcriptional elongation within the serum response network. Nat Struct Mol Biol (2010) 2.70
Messenger RNAs are recruited for nuclear export during transcription. Genes Dev (2001) 2.68
Transcription termination by nuclear RNA polymerases. Genes Dev (2009) 2.65
The beta -globin locus control region (LCR) functions primarily by enhancing the transition from transcription initiation to elongation. Genes Dev (2003) 2.65
Regulation of an intergenic transcript controls adjacent gene transcription in Saccharomyces cerevisiae. Genes Dev (2005) 2.64
TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II. Mol Cell (2009) 2.61
The transcription cycle of RNA polymerase II in living cells. J Cell Biol (2002) 2.59
Yeast Sen1 helicase protects the genome from transcription-associated instability. Mol Cell (2011) 2.59
The parafibromin tumor suppressor protein is part of a human Paf1 complex. Mol Cell Biol (2005) 2.58
The RNA polymerase II CTD coordinates transcription and RNA processing. Genes Dev (2012) 2.55
CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1. Genes Dev (2010) 2.52
Analysis of a noncanonical poly(A) site reveals a tripartite mechanism for vertebrate poly(A) site recognition. Genes Dev (2005) 2.52
C-terminal repeat domain kinase I phosphorylates Ser2 and Ser5 of RNA polymerase II C-terminal domain repeats. J Biol Chem (2004) 2.42
Functional integration of transcriptional and RNA processing machineries. Curr Opin Cell Biol (2008) 2.38
CDK-9/cyclin T (P-TEFb) is required in two postinitiation pathways for transcription in the C. elegans embryo. Genes Dev (2002) 2.36
The glucocorticoid receptor blocks P-TEFb recruitment by NFkappaB to effect promoter-specific transcriptional repression. Genes Dev (2005) 2.29
Variant histone H3.3 is deposited at sites of nucleosomal displacement throughout transcribed genes while active histone modifications show a promoter-proximal bias. Genes Dev (2005) 2.26
Two cyclin-dependent kinases promote RNA polymerase II transcription and formation of the scaffold complex. Mol Cell Biol (2004) 2.26
Gene-specific RNA polymerase II phosphorylation and the CTD code. Nat Struct Mol Biol (2010) 2.22
TFIIH-associated Cdk7 kinase functions in phosphorylation of C-terminal domain Ser7 residues, promoter-proximal pausing, and termination by RNA polymerase II. Mol Cell Biol (2009) 2.20
Biochemical analysis of TREX complex recruitment to intronless and intron-containing yeast genes. EMBO J (2004) 2.17
Sequential entry of components of the gene expression machinery into daughter nuclei. Mol Biol Cell (2003) 2.15
Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells. Genome Biol (2005) 2.11
Coupling mRNA processing with transcription in time and space. Nat Rev Genet (2014) 2.09
Transcription elongation controls cell fate specification in the Drosophila embryo. Genes Dev (2007) 2.08
Bur1 kinase is required for efficient transcription elongation by RNA polymerase II. Mol Cell Biol (2003) 2.08
Phosphorylation of the Pol II CTD by KIN28 enhances BUR1/BUR2 recruitment and Ser2 CTD phosphorylation near promoters. Mol Cell (2009) 2.08
RNA polymerase II accumulation in the promoter-proximal region of the dihydrofolate reductase and gamma-actin genes. Mol Cell Biol (2003) 2.07
RNA degradation in Saccharomyces cerevisae. Genetics (2012) 2.06
A Requirement for the Saccharomyces cerevisiae Paf1 complex in snoRNA 3' end formation. Mol Cell (2005) 2.05
The HRPT2 tumor suppressor gene product parafibromin associates with human PAF1 and RNA polymerase II. Mol Cell Biol (2005) 2.03
The nuclear RNA polymerase II surveillance system targets polymerase III transcripts. EMBO J (2011) 1.99
A histone H2A deubiquitinase complex coordinating histone acetylation and H1 dissociation in transcriptional regulation. Mol Cell (2007) 1.98
RNA polymerase II carboxy-terminal domain kinases: emerging clues to their function. Eukaryot Cell (2002) 1.90
Phosphorylation of the yeast Rpb1 C-terminal domain at serines 2, 5, and 7. J Biol Chem (2009) 1.89
RNAPol-ChIP: a novel application of chromatin immunoprecipitation to the analysis of real-time gene transcription. Nucleic Acids Res (2004) 1.86
Erk1/2 activity promotes chromatin features and RNAPII phosphorylation at developmental promoters in mouse ESCs. Cell (2014) 1.85
Global impact of RNA polymerase II elongation inhibition on alternative splicing regulation. Genome Res (2010) 1.80
Functional interactions between the transcription and mRNA 3' end processing machineries mediated by Ssu72 and Sub1. Genes Dev (2003) 1.79
The transition from transcriptional initiation to elongation. Curr Opin Genet Dev (2008) 1.78
The Drosophila BRM complex facilitates global transcription by RNA polymerase II. EMBO J (2002) 1.78
Modifications of RNA polymerase II are pivotal in regulating gene expression states. EMBO Rep (2009) 1.78
Splicing speckles are not reservoirs of RNA polymerase II, but contain an inactive form, phosphorylated on serine2 residues of the C-terminal domain. Mol Biol Cell (2006) 1.77
Genetic interactions of Spt4-Spt5 and TFIIS with the RNA polymerase II CTD and CTD modifying enzymes in Saccharomyces cerevisiae. Genetics (2001) 1.77
Chemical-genomic dissection of the CTD code. Nat Struct Mol Biol (2010) 1.76
The Myc transactivation domain promotes global phosphorylation of the RNA polymerase II carboxy-terminal domain independently of direct DNA binding. Mol Cell Biol (2007) 1.75
Global transcriptional repression in C. elegans germline precursors by regulated sequestration of TAF-4. Cell (2008) 1.74
Sustained induction of NF-kappa B is required for efficient expression of latent human immunodeficiency virus type 1. J Virol (2007) 1.74
A physiological role for gene loops in yeast. Genes Dev (2009) 1.73
Control of transcriptional elongation and cotranscriptional histone modification by the yeast BUR kinase substrate Spt5. Proc Natl Acad Sci U S A (2009) 1.73
Regulation of histone modification and cryptic transcription by the Bur1 and Paf1 complexes. EMBO J (2007) 1.73
A splicing-dependent transcriptional checkpoint associated with prespliceosome formation. Mol Cell (2014) 1.73
Transcription-independent functions of MYC: regulation of translation and DNA replication. Nat Rev Mol Cell Biol (2008) 1.72
Independent functions of yeast Pcf11p in pre-mRNA 3' end processing and in transcription termination. EMBO J (2003) 1.72
Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1. Mol Cell (2006) 1.71
Splicing and transcription-associated proteins PSF and p54nrb/nonO bind to the RNA polymerase II CTD. RNA (2002) 1.71
Chemical inhibition of the TFIIH-associated kinase Cdk7/Kin28 does not impair global mRNA synthesis. Proc Natl Acad Sci U S A (2007) 1.71
A genome-wide analysis indicates that yeast pre-mRNA splicing is predominantly posttranscriptional. Mol Cell (2006) 1.71
Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex. Proc Natl Acad Sci U S A (2004) 1.70
Biogenesis of mRNPs: integrating different processes in the eukaryotic nucleus. Chromosoma (2008) 1.69
Expanding the functional repertoire of CTD kinase I and RNA polymerase II: novel phosphoCTD-associating proteins in the yeast proteome. Biochemistry (2004) 1.68
Rtr1 is a CTD phosphatase that regulates RNA polymerase II during the transition from serine 5 to serine 2 phosphorylation. Mol Cell (2009) 1.68
TFIIH and P-TEFb coordinate transcription with capping enzyme recruitment at specific genes in fission yeast. Mol Cell (2009) 1.66
Ssu72 protein mediates both poly(A)-coupled and poly(A)-independent termination of RNA polymerase II transcription. Mol Cell Biol (2003) 1.64
TFIIH: when transcription met DNA repair. Nat Rev Mol Cell Biol (2012) 1.63
Activation of cardiac Cdk9 represses PGC-1 and confers a predisposition to heart failure. EMBO J (2004) 1.62
Phosphorylation of the RNA polymerase II carboxy-terminal domain by the Bur1 cyclin-dependent kinase. Mol Cell Biol (2001) 1.61
Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation. Mol Cell Biol (2002) 1.61
A Smad action turnover switch operated by WW domain readers of a phosphoserine code. Genes Dev (2011) 1.60
Dissecting the regulatory circuitry of a eukaryotic genome. Cell (1998) 23.62
The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. Nature (1997) 8.86
Analysis of chromatin structure by in vivo formaldehyde cross-linking. Methods (1997) 7.08
5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II. Genes Dev (1997) 6.68
Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme. Nature (1999) 6.20
RNA polymerase II and the integration of nuclear events. Genes Dev (2000) 6.16
mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain. Genes Dev (1997) 5.77
RNA polymerase II is an essential mRNA polyadenylation factor. Nature (1998) 4.87
In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes. Proc Natl Acad Sci U S A (1993) 4.82
Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains. J Cell Biol (1995) 4.13
Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases. Mol Cell (1998) 4.09
Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J Biol Chem (1989) 3.90
Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation. Trends Biochem Sci (2000) 3.77
Distinct roles for CTD Ser-2 and Ser-5 phosphorylation in the recruitment and allosteric activation of mammalian mRNA capping enzyme. Mol Cell (1999) 3.73
Nuclear bodies: multifaceted subdomains of the interchromatin space. Trends Cell Biol (1999) 3.49
Hrp1, a sequence-specific RNA-binding protein that shuttles between the nucleus and the cytoplasm, is required for mRNA 3'-end formation in yeast. Genes Dev (1997) 3.47
Messenger RNA synthesis in mammalian cells is catalyzed by the phosphorylated form of RNA polymerase II. J Biol Chem (1987) 3.29
Growth-related changes in phosphorylation of yeast RNA polymerase II. J Biol Chem (1998) 3.24
Mammalian capping enzyme complements mutant Saccharomyces cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA polymerase II. Proc Natl Acad Sci U S A (1997) 3.14
Phosphorylated RNA polymerase II stimulates pre-mRNA splicing. Genes Dev (1999) 3.00
Locus-specific variation in phosphorylation state of RNA polymerase II in vivo: correlations with gene activity and transcript processing. Genes Dev (1993) 2.82
Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes. Mol Biol Cell (1999) 2.80
Reconstitution of the transcription factor TFIIH: assignment of functions for the three enzymatic subunits, XPB, XPD, and cdk7. Mol Cell (1999) 2.67
Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol (2000) 2.64
In vitro transcription from the adenovirus 2 major late promoter utilizing templates truncated at promoter-proximal sites. J Biol Chem (1984) 2.58
The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay. Mol Cell (2000) 2.50
Phosphorylation of C-terminal domain of RNA polymerase II is not required in basal transcription. Nature (1993) 2.43
Capping enzyme in eukaryotic mRNA synthesis. Prog Nucleic Acid Res Mol Biol (1995) 2.42
Control of cleavage site selection during mRNA 3' end formation by a yeast hnRNP. EMBO J (1998) 2.21
The role of multisite phosphorylation in the regulation of RNA polymerase II activity. Prog Nucleic Acid Res Mol Biol (1994) 2.21
A kinase-deficient transcription factor TFIIH is functional in basal and activated transcription. Proc Natl Acad Sci U S A (1995) 2.20
Transcription units as RNA processing units. Genes Dev (1997) 2.14
Messenger RNA capping enzymes from eukaryotic cells. Prog Nucleic Acid Res Mol Biol (1987) 1.98
Phospho-carboxyl-terminal domain binding and the role of a prolyl isomerase in pre-mRNA 3'-End formation. J Biol Chem (1999) 1.95
The Ess1 prolyl isomerase is linked to chromatin remodeling complexes and the general transcription machinery. EMBO J (2000) 1.88
Evidence for a mediator cycle at the initiation of transcription. Proc Natl Acad Sci U S A (1997) 1.60
Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes. J Biol Chem (1998) 1.44
A specific RNA-protein interaction at yeast polyadenylation efficiency elements. Nucleic Acids Res (1998) 1.34
DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs. Genes Dev (1998) 6.96
mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain. Genes Dev (1997) 5.77
Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain. Genes Dev (2001) 4.64
Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA sequences. Proc Natl Acad Sci U S A (1989) 3.18
Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions. Cell (1989) 3.18
Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair. Cell (1993) 2.92
Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol (2000) 2.64
Bromodomain factor 1 corresponds to a missing piece of yeast TFIID. Genes Dev (2000) 2.47
A novel transcription factor reveals a functional link between the RNA polymerase II CTD and TFIID. Cell (1992) 1.98
An RNA 5'-triphosphatase related to the protein tyrosine phosphatases. Cell (1997) 1.91
Active site of the mRNA-capping enzyme guanylyltransferase from Saccharomyces cerevisiae: similarity to the nucleotidyl attachment motif of DNA and RNA ligases. Proc Natl Acad Sci U S A (1994) 1.68
Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain. Genes Dev (1998) 1.49
Conditional mutants of the yeast mRNA capping enzyme show that the cap enhances, but is not required for, mRNA splicing. RNA (1996) 1.46
Histone-like TAFs are essential for transcription in vivo. Mol Cell (1998) 1.43
The yeast TFB1 and SSL1 genes, which encode subunits of transcription factor IIH, are required for nucleotide excision repair and RNA polymerase II transcription. Mol Cell Biol (1995) 1.38
Evidence that transcription factor IIB is required for a post-assembly step in transcription initiation. J Biol Chem (1999) 1.33
A histone fold TAF octamer within the yeast TFIID transcriptional coactivator. Nat Struct Biol (2001) 1.33
Yeast homologues of higher eukaryotic TFIID subunits. Proc Natl Acad Sci U S A (1996) 1.32
Specific manipulative therapy treatment for chronic lateral epicondylalgia produces uniquely characteristic hypoalgesia. Man Ther (2001) 1.32
Identification of a yeast protein homologous in function to the mammalian general transcription factor, TFIIA. EMBO J (1989) 1.25
Genetic analysis of the large subunit of yeast transcription factor IIE reveals two regions with distinct functions. Mol Cell Biol (1997) 1.23
A yeast protein possesses the DNA-binding properties of the adenovirus major late transcription factor. Mol Cell Biol (1989) 1.20
Tandem high-dose chemotherapy and autologous stem cell rescue in patients over 1 year of age with stage 4 neuroblastoma. Bone Marrow Transplant (2007) 1.19
The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein. Proc Natl Acad Sci U S A (1999) 1.16
The impact of post-thaw colony-forming units-granulocyte/macrophage on engraftment following unrelated cord blood transplantation in pediatric recipients. Bone Marrow Transplant (2007) 1.13
TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo. Genes Dev (1999) 1.10
A protein tyrosine phosphatase-like protein from baculovirus has RNA 5'-triphosphatase and diphosphatase activities. Proc Natl Acad Sci U S A (1998) 1.08
A Saccharomyces cerevisiae RNA 5'-triphosphatase related to mRNA capping enzyme. Nucleic Acids Res (1999) 1.08
A randomized, double-blind trial of palonosetron compared with ondansetron in preventing postoperative nausea and vomiting after gynaecological laparoscopic surgery. J Int Med Res (2011) 1.07
A nucleocytoplasmic malate dehydrogenase regulates p53 transcriptional activity in response to metabolic stress. Cell Death Differ (2009) 1.03
Antioxidative activity of green tea treated with radical initiator 2, 2'-azobis(2-amidinopropane) dihydrochloride. J Agric Food Chem (2000) 1.02
Human PIR1 of the protein-tyrosine phosphatase superfamily has RNA 5'-triphosphatase and diphosphatase activities. J Biol Chem (1999) 0.95
The essential interaction between yeast mRNA capping enzyme subunits is not required for triphosphatase function in vivo. Mol Cell Biol (2000) 0.94
The effects of Coptidis Rhizoma extract on a hypercholesterolemic animal model. Phytomedicine (2003) 0.94
An interaction between the Tfb1 and Ssl1 subunits of yeast TFIIH correlates with DNA repair activity. Nucleic Acids Res (1995) 0.93
Common themes in translational and transcriptional regulation. Trends Biochem Sci (1997) 0.91
Tandem high-dose chemotherapy and autologous stem cell transplantation in patients with high-risk neuroblastoma: results of SMC NB-2004 study. Bone Marrow Transplant (2012) 0.90
Tandem high-dose chemotherapy and autologous stem cell rescue in children with bilateral advanced retinoblastoma. Bone Marrow Transplant (2008) 0.89
Anti-aging effects of oligomeric proanthocyanidins isolated from persimmon fruits. Drug Discov Ther (2011) 0.82
A Plasmodium falciparum protein related to fungal RNA 5'-triphosphatases. Mol Biochem Parasitol (2001) 0.82
Effects of green tea tannin on cisplatin-induced nephropathy in LLC-PK1 cells and rats. J Pharm Pharmacol (1999) 0.79
Effect of Wen-Pi-Tang extract on lung damage by influenza virus infection. Phytomedicine (2004) 0.79
Successive double high-dose chemotherapy with peripheral blood stem cell rescue collected during a single leukapheresis round in patients with high-risk pediatric solid tumors: a pilot study in a single center. Bone Marrow Transplant (2003) 0.77
Evaluation of the peroxynitrite scavenging activity of heat-processed ginseng. J Med Food (2009) 0.76
Diagnosis of bovine freemartinism by fluorescence in situ hybridization on interphase nuclei using a bovine Y chromosome-specific DNA probe. Theriogenology (2007) 0.76
A study on setting of the fatigue limit of temporary dental implants. Conf Proc IEEE Eng Med Biol Soc (2017) 0.75
Purification of DNA-binding proteins using biotin/streptavidin affinity systems. Curr Protoc Protein Sci (2001) 0.75