Published in Biochemistry on December 26, 2000
Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility. Nucleic Acids Res (2001) 3.04
Mutations altering the cleavage specificity of a homing endonuclease. Nucleic Acids Res (2002) 1.60
Engineering variants of the I-SceI homing endonuclease with strand-specific and site-specific DNA-nicking activity. J Mol Biol (2008) 1.24
Evolution of divergent DNA recognition specificities in VDE homing endonucleases from two yeast species. Nucleic Acids Res (2004) 0.99
Homing endonucleases: from genetic anomalies to programmable genomic clippers. Methods Mol Biol (2014) 0.89
Degeneration of a homing endonuclease and its target sequence in a wild yeast strain. Nucleic Acids Res (2001) 0.86
The Ins(1,3,4)P3 5/6-kinase/Ins(3,4,5,6)P4 1-kinase is not a protein kinase. Biochem J (2005) 0.84
Type II restriction endonucleases: structure and mechanism. Cell Mol Life Sci (2005) 4.15
Activation of ternary complex factor Elk-1 by MAP kinases. EMBO J (1993) 3.47
Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site. Biochemistry (1984) 3.03
c-fos transcriptional activation and repression correlate temporally with the phosphorylation status of TCF. EMBO J (1993) 1.82
Horizontal gene transfer contributes to the wide distribution and evolution of type II restriction-modification systems. J Mol Evol (1996) 1.82
The interaction of the EcoRI restriction endonuclease with its substrate. A physico-chemical study employing natural and synthetic oligonucleotides and polynucleotides. Eur J Biochem (1980) 1.75
Influence of DNA target melting behavior on real-time PCR quantification. Clin Chem (2000) 1.66
A site-directed mutagenesis study to identify amino acid residues involved in the catalytic function of the restriction endonuclease EcoRV. Biochemistry (1992) 1.62
Linear diffusion of restriction endonucleases on DNA. J Biol Chem (1985) 1.54
Ternary complex formation between elongation factor Tu, GTP and aminoacyl-tRNA: an equilibrium study. Eur J Biochem (1977) 1.50
Pausing of the restriction endonuclease EcoRI during linear diffusion on DNA. Biochemistry (1994) 1.46
Activation of vascular smooth muscle of rat aorta by noradrenaline and depolarization: two different mechanisms. Pflugers Arch (1971) 1.44
Kinetic characterization of linear diffusion of the restriction endonuclease EcoRV on DNA. Biochemistry (1998) 1.39
Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases. Nucleic Acids Res (1986) 1.34
Linear diffusion of the restriction endonuclease EcoRV on DNA is essential for the in vivo function of the enzyme. EMBO J (1996) 1.34
The stereochemical course of the restriction endonuclease EcoRI-catalyzed reaction. J Biol Chem (1984) 1.33
Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis. Biochemistry (1991) 1.32
Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes. Proc Natl Acad Sci U S A (1993) 1.31
Accuracy of the EcoRI restriction endonuclease: binding and cleavage studies with oligodeoxynucleotide substrates containing degenerate recognition sequences. Biochemistry (1990) 1.31
The influence of sequences adjacent to the recognition site on the cleavage of oligodeoxynucleotides by the EcoRI endonuclease. Eur J Biochem (1984) 1.30
Equivalent and non-equivalent binding sites for tRNA on aminoacyl-tRNA synthetases. Eur J Biochem (1975) 1.29
Mg2+ confers DNA binding specificity to the EcoRV restriction endonuclease. Biochemistry (1992) 1.27
Inhibition of Eco RI action by polynucleotides. A characterization of the non-specific binding of the enzyme to DNA. Nucleic Acids Res (1980) 1.25
Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques. Anal Biochem (1991) 1.20
Evidence for an evolutionary relationship among type-II restriction endonucleases. Gene (1995) 1.19
Sau3AI, a monomeric type II restriction endonuclease that dimerizes on the DNA and thereby induces DNA loops. J Biol Chem (2001) 1.18
A fast and accurate enzyme-linked immunosorbent assay for the determination of the DNA cleavage activity of restriction endonucleases. Anal Biochem (1993) 1.16
Cross-linking of bromodeoxyuridine-substituted oligonucleotides to the EcoRI and EcoRV restriction endonucleases. Eur J Biochem (1986) 1.16
The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate. EMBO J (1999) 1.15
A similar active site for non-specific and specific endonucleases. Nat Struct Biol (1999) 1.15
A comparison of the structural requirements for DNA cleavage by the isoschizomers HaeIII, BspRI and BsuRI. Eur J Biochem (1985) 1.14
On the catalytic mechanism of EcoRI and EcoRV. A detailed proposal based on biochemical results, structural data and molecular modelling. FEBS Lett (1992) 1.14
Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis. Nucleic Acids Res (1996) 1.13
Distinct steps in the specific binding of tRNA to aminoacyl-tRNA synthetase. Temperature-jump studies on the serine-specific system from yeast and the tyrosine-specific system from Escherichia coli. Eur J Biochem (1976) 1.11
Introduction of asymmetry in the naturally symmetric restriction endonuclease EcoRV to investigate intersubunit communication in the homodimeric protein. Proc Natl Acad Sci U S A (1996) 1.10
Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI. Nucleic Acids Res (1986) 1.10
The elongation factor Tu binds aminoacyl-tRNA in the presence of GDP. J Biol Chem (1982) 1.09
Transient cleavage kinetics of the Eco RI restriction endonuclease measured in a pulsed quench-flow apparatus: enzyme concentration-dependent activity change. Nucleic Acids Res (1981) 1.09
Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides. Nucleic Acids Res (1988) 1.08
On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target. Protein Eng (2000) 1.06
The effect of several nucleic acid binding drugs on the cleavage of d(GGAATTCC) and pBR 322 by the Eco RI restriction endonuclease. Nucleic Acids Res (1981) 1.05
Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-SceI. Nucleic Acids Res (1996) 1.03
Probing the indirect readout of the restriction enzyme EcoRV. Mutational analysis of contacts to the DNA backbone. J Biol Chem (1996) 1.03
DNA binding specificity of the EcoRV restriction endonuclease is increased by Mg2+ binding to a metal ion binding site distinct from the catalytic center of the enzyme. Biochemistry (1995) 1.03
Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye. Anal Biochem (1991) 1.01
Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases. Biochemistry (1984) 1.00
Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis. Nucleic Acids Res (1994) 0.99
Does the restriction endonuclease EcoRV employ a two-metal-Ion mechanism for DNA cleavage? Biochemistry (1997) 0.99
Aminoacyl transfer ribonucleic acid binding site of the bacterial elongation factor Tu. Biochemistry (1980) 0.98
Polymerase chain reaction detection of a highly polymorphic VNTR segment in intron 1 of the human p53 gene. Clin Chem (1993) 0.98
Spermidine increases the accuracy of type II restriction endonucleases. Suppression of cleavage at degenerate, non-symmetrical sites. Eur J Biochem (1985) 0.97
The recognition of methylated DNA by the GTP-dependent restriction endonuclease McrBC resides in the N-terminal domain of McrB. Biol Chem (1997) 0.95
A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. Protein Expr Purif (1994) 0.95
Sequence preferences in cleavage of dsDNA and ssDNA by the extracellular Serratia marcescens endonuclease. Biochemistry (1995) 0.95
Engineering novel restriction endonucleases: principles and applications. Trends Biotechnol (1996) 0.94
Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease. Eur J Biochem (1996) 0.94
The role of translocation in ribosomal accuracy. Translocation rates for cognate and noncognate aminoacyl- and peptidyl-tRNAs on Escherichia coli ribosomes. J Biol Chem (1987) 0.94
Does the specific recognition of DNA by the restriction endonuclease EcoRI involve a linear diffusion step? Investigation of the processivity of the EcoRI endonuclease. Nucleic Acids Res (1983) 0.93
Fluorescence stopped-flow kinetics of the cleavage of synthetic oligodeoxynucleotides by the EcoRI restriction endonuclease. Biochemistry (1989) 0.93
Biochemical characterization of Anabaena sp. strain PCC 7120 non-specific nuclease NucA and its inhibitor NuiA. Eur J Biochem (1998) 0.93
Semiautomated quantitative detection of loss of heterozygosity in the tumor suppressor gene p53. Biotechniques (1995) 0.92
Characterization of the interaction between the restriction endonuclease McrBC from E. coli and its cofactor GTP. J Mol Biol (1997) 0.92
Two identical subunits of the EcoRI restriction endonuclease Co-operate in the binding and cleavage of the palindromic substrate. Eur J Biochem (1982) 0.92
On the advantage of being a dimer, a case study using the dimeric Serratia nuclease and the monomeric nuclease from Anabaena sp. strain PCC 7120. J Biol Chem (1999) 0.91
Protein engineering of the restriction endonuclease EcoRV: replacement of an amino acid residue in the DNA binding site leads to an altered selectivity towards unmodified and modified substrates. Biochim Biophys Acta (1994) 0.91
The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding. Nucleic Acids Res (1998) 0.91
The production and characterization of artificial heterodimers of the restriction endonuclease EcoRV. Biol Chem (1996) 0.91
Fluoresceinylthiocarbamyl-tRNATyr: a useful derivative of tRNATyr (E.coli) for physicochemical studies. Nucleic Acids Res (1977) 0.90
Adenylate kinases from thermosensitive Escherichia coli strains. J Mol Biol (1989) 0.90
Protein engineering of the restriction endonuclease EcoRV--structure-guided design of enzyme variants that recognize the base pairs flanking the recognition site. Eur J Biochem (1998) 0.90
Identification of functionally relevant histidine residues in the apoptotic nuclease CAD. Nucleic Acids Res (2001) 0.90
Plasmid DNA cleavage by MunI restriction enzyme: single-turnover and steady-state kinetic analysis. Biochemistry (1999) 0.90
Reduction of the size of acute, experimental myocardial infarction by Verapamil. Basic Res Cardiol (1975) 0.89
The pharmacokinetics, bioequivalence and bioavailability of different formulations of metoclopramide in man. Arzneimittelforschung (1981) 0.89
Determination of the DNA bend angle induced by the restriction endonuclease EcoRV in the presence of Mg2+. J Biol Chem (1993) 0.89
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis (2001) 0.88
How many EF-Tu molecules participate in aminoacyl-tRNA binding? Biochimie (1992) 0.88
Photocross-linking of the homing endonuclease PI-SceI to its recognition sequence. J Biol Chem (1999) 0.88
Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude. J Mol Biol (1998) 0.87
The binding of kirromycin to elongation factor Tu. Structural alterations are responsible for the inhibitory action. Eur J Biochem (1978) 0.87
Engineering of variants of the restriction endonuclease EcoRV that depend in their cleavage activity on the flexibility of sequences flanking the recognition site. Biochemistry (1998) 0.86
The antibiotics kirromycin and pulvomycin bind to different sites on the elongation factor Tu from Escherichia coli. Eur J Biochem (1982) 0.86