Published in J Bacteriol on September 01, 1979
Uses of lac fusions for the study of biological problems. Microbiol Rev (1985) 4.38
In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage. Proc Natl Acad Sci U S A (1984) 3.06
A mechanism of protein localization: the signal hypothesis and bacteria. J Cell Biol (1980) 2.91
Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli. Genetics (1980) 2.63
Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA. J Bacteriol (1981) 2.38
The nucleotide sequence of the promoter and the amino-terminal region of alkaline phosphatase structural gene (phoA) of Escherichia coli. Nucleic Acids Res (1981) 2.32
Signal sequence of alkaline phosphatase of Escherichia coli. J Bacteriol (1982) 2.31
Effects of signal sequence mutations on the kinetics of alkaline phosphatase export to the periplasm in Escherichia coli. J Bacteriol (1986) 2.17
In vitro construction and characterization of phoA-lacZ gene fusions in Escherichia coli. J Bacteriol (1983) 1.82
Use of gene fusions to determine the orientation of gene phoA on the Escherichia coli chromosome. J Bacteriol (1981) 1.74
Positions of early nonsense and deletion mutations in lacZ. J Bacteriol (1980) 1.39
Cloning and characterization of the Escherichia coli gene coding for alkaline phosphatase. J Bacteriol (1981) 1.29
Retroviral gag gene amber codon suppression is caused by an intrinsic cis-acting component of the viral mRNA. J Virol (1988) 1.12
Kinetics and regulation of cell-free alkaline phosphatase synthesis. J Bacteriol (1980) 1.02
Effect of glpT and glpD mutations on expression of the phoA gene in Escherichia coli. J Bacteriol (1993) 0.96
Expression of a copy number control gene (copB) of plasmid R1 is constitutive and growth rate dependent. J Bacteriol (1982) 0.92
Alkaline phosphatase secretion-negative mutant of Bacillus licheniformis 749/C. J Bacteriol (1983) 0.84
Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (1970) 1528.65
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol (1975) 36.95
Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev (1976) 35.30
Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol (1976) 33.01
Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta (1960) 14.35
Intracellular protein degradation in mammalian and bacterial cells: Part 2. Annu Rev Biochem (1976) 12.05
Phenanthrenequinone as an analytical reagent for arginine and other monosubstituted guanidines. Biochim Biophys Acta (1966) 7.11
Amino acid sequence for the peptide extension on the prolipoprotein of the Escherichia coli outer membrane. Proc Natl Acad Sci U S A (1977) 6.22
Studies on bacteriophage fd DNA. IV. The sequence of messenger RNA for the major coat protein gene. J Mol Biol (1977) 5.34
Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro. Proc Natl Acad Sci U S A (1977) 5.29
Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K. Proc Natl Acad Sci U S A (1978) 5.11
High-level production of -galactosidase by Escherichia coli merodiploids. J Bacteriol (1972) 4.83
Isolation and properties of bacteria capable of high rates of beta-galactosidase synthesis. Biochim Biophys Acta (1962) 3.93
Use of gene fusions to study outer membrane protein localization in Escherichia coli. Proc Natl Acad Sci U S A (1977) 3.78
Synthesis of exported proteins by membrane-bound polysomes from Escherichia coli. Eur J Biochem (1977) 3.74
Extracellular labeling of nascent polypeptides traversing the membrane of Escherichia coli. Proc Natl Acad Sci U S A (1977) 3.16
Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm. Nature (1979) 3.10
Beta-galactosidase. Rates of synthesis and degradation of incomplete chains. J Biol Chem (1972) 3.06
Amino acid sequence of beta-galactosidase. XI. Peptide ordering procedures and the complete sequence. J Biol Chem (1978) 2.51
Alkaline phosphatase of Escherichia coli: a zinc metalloenzyme. Biochemistry (1962) 2.11
beta-Galactosidase chimeras: primary structure of a lac repressor-beta-galactosidase protein. Proc Natl Acad Sci U S A (1978) 1.60
Amino acid sequence of beta-galactosidase. IV. Sequence of an alpha-complementing cyanogen bromide peptide, residues 3 to 92. J Biol Chem (1975) 1.54
Amino acid sequence of Escherichia coli alkaline phosphatase. Amino- and carboxyl-terminal sequences and variations between two isozymes. Biochemistry (1973) 1.51
A dominant constitutive phoR mutation in Escherichia coli. Genetics (1972) 1.34
Studies on the role of bacteriophage T7 lysozyme during phage infection. J Mol Biol (1975) 1.24
Position of the mutation in beta-galactosidase ochre mutant U118. J Bacteriol (1978) 1.23
Amino acid sequence of beta-galactosidase. VII. Isolation of the 24 cyanogen bromide peptides. J Biol Chem (1978) 1.12
Mechanistic aspects of the transfer of nascent periplasmic proteins across the cytoplasmic membrane in Escherichia coli. Eur J Biochem (1978) 1.10
An immunochemical aid to sequence determination of proteins. Anal Biochem (1977) 0.96
Position of the extra amino acid sequence in the precursor arabinose-binding protein of Escherichia coli. J Bacteriol (1978) 0.81
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol (1995) 46.37
TnphoA: a transposon probe for protein export signals. Proc Natl Acad Sci U S A (1985) 21.06
Discrimination as a consequence of genetic testing. Am J Hum Genet (1992) 10.40
Mechanism of activation of catabolite-sensitive genes: a positive control system. Proc Natl Acad Sci U S A (1970) 9.37
A genetic approach to analyzing membrane protein topology. Science (1986) 9.01
Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages. J Mol Biol (1975) 8.79
Identification of a protein required for disulfide bond formation in vivo. Cell (1991) 8.56
Protein localization in E. coli: is there a common step in the secretion of periplasmic and outer-membrane proteins? Cell (1981) 8.24
Mechanism of incorporation of cell envelope proteins in Escherichia coli. Annu Rev Microbiol (1982) 8.18
E. coli mutant pleiotropically defective in the export of secreted proteins. Cell (1981) 7.72
An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins. Proc Natl Acad Sci U S A (1988) 6.31
Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli. J Bacteriol (1983) 6.27
Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives. Mol Gen Genet (1986) 5.87
Determinants of membrane protein topology. Proc Natl Acad Sci U S A (1987) 5.76
Regulation of a membrane component required for protein secretion in Escherichia coli. Cell (1982) 5.70
Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro. Proc Natl Acad Sci U S A (1977) 5.29
Regulation of metallothionein--thymidine kinase fusion plasmids injected into mouse eggs. Nature (1982) 5.14
The use of transposon TnphoA to detect genes for cell envelope proteins subject to a common regulatory stimulus. Analysis of osmotically regulated genes in Escherichia coli. J Mol Biol (1987) 4.98
Localization of FtsI (PBP3) to the septal ring requires its membrane anchor, the Z ring, FtsA, FtsQ, and FtsL. J Bacteriol (1999) 4.87
Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli. Nature (1980) 4.77
Escherichia coli pleiotropic mutant that reduces amounts of several periplasmic and outer membrane proteins. J Bacteriol (1979) 4.69
New controlling element in the Lac operon of E. coli. Nature (1968) 4.63
Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature. J Bacteriol (1989) 4.56
Molecular basis of beta-galactosidase alpha-complementation. Proc Natl Acad Sci U S A (1975) 4.38
Mutations in a new gene, secB, cause defective protein localization in Escherichia coli. J Bacteriol (1983) 4.35
Structure of the cell wall anchor of surface proteins in Staphylococcus aureus. Science (1995) 4.27
A phase I evaluation of the safety and immunogenicity of vaccination with recombinant gp160 in patients with early human immunodeficiency virus infection. Military Medical Consortium for Applied Retroviral Research. N Engl J Med (1991) 4.23
Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase. J Bacteriol (1973) 4.03
Evidence for specificity at an early step in protein export in Escherichia coli. J Bacteriol (1985) 3.93
A pathway for disulfide bond formation in vivo. Proc Natl Acad Sci U S A (1993) 3.86
Isolation of pure lac operon DNA. Nature (1969) 3.79
Use of gene fusions to study outer membrane protein localization in Escherichia coli. Proc Natl Acad Sci U S A (1977) 3.78
Genetic analysis of protein export in Escherichia coli. Annu Rev Genet (1990) 3.73
The amino acid sequence of beta-galactosidase of Escherichia coli. Proc Natl Acad Sci U S A (1977) 3.63
Who should own scientific papers? Science (1998) 3.62
Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proc Natl Acad Sci U S A (1999) 3.60
Regulation of the OxyR transcription factor by hydrogen peroxide and the cellular thiol-disulfide status. Proc Natl Acad Sci U S A (1999) 3.60
Alkaline phosphatase fusions: sensors of subcellular location. J Bacteriol (1990) 3.59
FtsL, an essential cytoplasmic membrane protein involved in cell division in Escherichia coli. J Bacteriol (1992) 3.50
Evidence for two sites in the lac promoter region. J Mol Biol (1972) 3.44
Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli. Nature (1984) 3.42
The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm. J Biol Chem (1997) 3.42
Inactivation of FtsI inhibits constriction of the FtsZ cytokinetic ring and delays the assembly of FtsZ rings at potential division sites. Proc Natl Acad Sci U S A (1997) 3.25
The bonds that tie: catalyzed disulfide bond formation. Cell (1993) 3.20
The FtsQ protein of Escherichia coli: membrane topology, abundance, and cell division phenotypes due to overproduction and insertion mutations. J Bacteriol (1991) 3.15
Towards single-copy gene expression systems making gene cloning physiologically relevant: lambda InCh, a simple Escherichia coli plasmid-chromosome shuttle system. J Bacteriol (2000) 3.14
Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12. J Biol Chem (1984) 3.10
Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm. Nature (1979) 3.10
Deletion of the Escherichia coli crp gene. J Bacteriol (1975) 3.08
Beta-galactosidase. Rates of synthesis and degradation of incomplete chains. J Biol Chem (1972) 3.06
SecD and SecF facilitate protein export in Escherichia coli. EMBO J (1994) 3.04
A bacterial virulence determinant encoded by lysogenic coliphage lambda. Nature (1990) 3.02
An in vivo pathway for disulfide bond isomerization in Escherichia coli. Proc Natl Acad Sci U S A (1996) 2.98
Regulation of Escherichia coli cell envelope proteins involved in protein folding and degradation by the Cpx two-component system. Genes Dev (1997) 2.96
Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro. J Bacteriol (1981) 2.88
secD, a new gene involved in protein export in Escherichia coli. J Bacteriol (1987) 2.84
The nucleotide sequence of the gene for malF protein, an inner membrane component of the maltose transport system of Escherichia coli. Repeated DNA sequences are found in the malE-malF intercistronic region. J Biol Chem (1984) 2.79
Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli. Science (1993) 2.73
Co-linearity of beta-galactosidase with its gene by immunological detection of incomplete polypeptide chains. Science (1966) 2.71
A signal sequence is not required for protein export in prlA mutants of Escherichia coli. EMBO J (1993) 2.70
Roles of thiol-redox pathways in bacteria. Annu Rev Microbiol (2001) 2.60
SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF. Cell (1995) 2.58
Disulfide bond formation in the Escherichia coli cytoplasm: an in vivo role reversal for the thioredoxins. EMBO J (1998) 2.56
Amino acid sequence of beta-galactosidase. XI. Peptide ordering procedures and the complete sequence. J Biol Chem (1978) 2.51
Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm. J Bacteriol (1991) 2.42
The secD locus of E.coli codes for two membrane proteins required for protein export. EMBO J (1990) 2.40
Use of phoA fusions to study the topology of the Escherichia coli inner membrane protein leader peptidase. J Bacteriol (1989) 2.38
Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA. J Bacteriol (1981) 2.38
Genetic analysis of the membrane insertion and topology of MalF, a cytoplasmic membrane protein of Escherichia coli. J Mol Biol (1988) 2.35
A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene. Genetics (1988) 2.34
FtsQ, FtsL and FtsI require FtsK, but not FtsN, for co-localization with FtsZ during Escherichia coli cell division. Mol Microbiol (2001) 2.32
Signal sequence of alkaline phosphatase of Escherichia coli. J Bacteriol (1982) 2.31
The secE gene encodes an integral membrane protein required for protein export in Escherichia coli. Genes Dev (1989) 2.27
Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli. J Bacteriol (1982) 2.27
Septal localization of FtsQ, an essential cell division protein in Escherichia coli. J Bacteriol (1999) 2.22
Intracellular trehalose improves the survival of cryopreserved mammalian cells. Nat Biotechnol (2000) 2.20
The role of charged amino acids in the localization of secreted and membrane proteins. Cell (1990) 2.19
Localization of the Escherichia coli cell division protein Ftsl (PBP3) to the division site and cell pole. Mol Microbiol (1997) 2.19
Effects of signal sequence mutations on the kinetics of alkaline phosphatase export to the periplasm in Escherichia coli. J Bacteriol (1986) 2.17
The genetics of disulfide bond metabolism. Annu Rev Genet (1998) 2.17
The organization of the araBAD operon of Escherichia coli. Gene (1986) 2.16
Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin. J Bacteriol (1997) 2.10
Domain-swapping analysis of FtsI, FtsL, and FtsQ, bitopic membrane proteins essential for cell division in Escherichia coli. J Bacteriol (1997) 2.07
DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region. Proc Natl Acad Sci U S A (1985) 2.05
Beta-galactosidase from termination and deletion mutant strains. J Bacteriol (1974) 2.05
Fusions of the lac operon to the transfer RNA gene tyrT of Escherichia coli. J Mol Biol (1979) 2.03
Protein complementation. Annu Rev Biochem (1975) 2.00
A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm. Nature (1980) 2.00
Alteration of the amino terminus of the mature sequence of a periplasmic protein can severely affect protein export in Escherichia coli. Proc Natl Acad Sci U S A (1988) 1.96
Molecular genetic analysis of membrane protein topology. Trends Genet (1988) 1.95
The amino acid sequence of -galactosidase. 3. The sequences of NH 2 - and COOH-terminal tryptic peptides. J Biol Chem (1972) 1.94
Direction of transcription of a regulatory gene in E. coli. Nature (1968) 1.94
Molecular basis of DNA sequence recognition by the catabolite gene activator protein: detailed inferences from three mutations that alter DNA sequence specificity. Proc Natl Acad Sci U S A (1984) 1.94
Evidence that the pathway of disulfide bond formation in Escherichia coli involves interactions between the cysteines of DsbB and DsbA. Proc Natl Acad Sci U S A (1995) 1.92
Residues essential for the function of SecE, a membrane component of the Escherichia coli secretion apparatus, are located in a conserved cytoplasmic region. Proc Natl Acad Sci U S A (1994) 1.92
Genetic analysis of membrane protein topology by a sandwich gene fusion approach. Proc Natl Acad Sci U S A (1990) 1.89