Published in Biosens Bioelectron on April 01, 2002
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The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nat Protoc (2007) 3.23
Crucial role for human Toll-like receptor 4 in the development of contact allergy to nickel. Nat Immunol (2010) 3.15
Engineered protein scaffolds as next-generation antibody therapeutics. Curr Opin Chem Biol (2009) 2.41
Interferon-gamma regulates intestinal epithelial homeostasis through converging beta-catenin signaling pathways. Immunity (2010) 2.39
Fluorescent substrates for soluble epoxide hydrolase and application to inhibition studies. Anal Biochem (2005) 2.26
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The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands. J Biol Chem (2004) 1.30
Structural insights into the antigenicity of myelin oligodendrocyte glycoprotein. Proc Natl Acad Sci U S A (2003) 1.27
The crystal structure of the bacteriophage PSA endolysin reveals a unique fold responsible for specific recognition of Listeria cell walls. J Mol Biol (2006) 1.26
Binding proteins from alternative scaffolds. J Immunol Methods (2004) 1.26
Artificial, non-antibody binding proteins for pharmaceutical and industrial applications. Trends Biotechnol (2005) 1.24
Temperature and pressure dependence of protein stability: the engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram. Proc Natl Acad Sci U S A (2008) 1.22
Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody. Proc Natl Acad Sci U S A (2010) 1.21
Comparative ligand-binding analysis of ten human lipocalins. Biochim Biophys Acta (2006) 1.21
Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site. Protein Sci (2002) 1.20
Development of a high-throughput screen for soluble epoxide hydrolase inhibition. Anal Biochem (2006) 1.19
A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Protein Eng Des Sel (2006) 1.16
Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture. Nat Struct Mol Biol (2004) 1.13
Structural insight into the dual ligand specificity and mode of high density lipoprotein association of apolipoprotein D. J Biol Chem (2007) 1.11
A new crystal form of human tear lipocalin reveals high flexibility in the loop region and induced fit in the ligand cavity. Acta Crystallogr D Biol Crystallogr (2009) 1.10
Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells. Mol Biol Cell (2009) 1.08
The 1.8-A crystal structure of alpha1-acid glycoprotein (Orosomucoid) solved by UV RIP reveals the broad drug-binding activity of this human plasma lipocalin. J Mol Biol (2008) 1.04
The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site. Mol Immunol (2007) 1.02
Structural analysis of the L-alanoyl-D-glutamate endopeptidase domain of Listeria bacteriophage endolysin Ply500 reveals a new member of the LAS peptidase family. Acta Crystallogr D Biol Crystallogr (2008) 1.01
Juvenile hormone (JH) esterase: why are you so JH specific? Insect Biochem Mol Biol (2003) 0.99
Lipocalins in drug discovery: from natural ligand-binding proteins to "anticalins". Drug Discov Today (2005) 0.99
Novel serial positive enrichment technology enables clinical multiparameter cell sorting. PLoS One (2012) 0.98
Structural mechanism of specific ligand recognition by a lipocalin tailored for the complexation of digoxigenin. J Mol Biol (2003) 0.97
Neutrophil gelatinase-associated lipocalin expresses antimicrobial activity by interfering with L-norepinephrine-mediated bacterial iron acquisition. Antimicrob Agents Chemother (2010) 0.97
Alternative binding proteins get mature: rivalling antibodies. FEBS J (2008) 0.97
PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins. Protein Eng Des Sel (2013) 0.97
Crystallographic analysis of an "anticalin" with tailored specificity for fluorescein reveals high structural plasticity of the lipocalin loop region. Proteins (2003) 0.96
Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: effects on biophysical properties and prolonged plasma half-life. Protein Eng Des Sel (2007) 0.96
Comparative analysis reveals selective recognition of glycans by the dendritic cell receptors DC-SIGN and Langerin. Protein Eng Des Sel (2011) 0.95
Identification of potential substrate proteins for the periplasmic Escherichia coli chaperone Skp. Proteomics (2008) 0.95
New insights into the signaling mechanism of the pH-responsive, membrane-integrated transcriptional activator CadC of Escherichia coli. J Biol Chem (2011) 0.93
Anticalins small engineered binding proteins based on the lipocalin scaffold. Methods Enzymol (2012) 0.92
Construction of an artificial receptor protein ("anticalin") based on the human apolipoprotein D. Chembiochem (2004) 0.90
The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops. Proteins (2008) 0.90
Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism. Acta Crystallogr Sect F Struct Biol Cryst Commun (2009) 0.89
Enzyme-linked immunosorbent assay (ELISA) and sol-gel-based immunoaffinity purification (IAP) of the pyrethroid bioallethrin in food and environmental samples. J Agric Food Chem (2006) 0.88
A disulfide-free single-domain V(L) intrabody with blocking activity towards huntingtin reveals a novel mode of epitope recognition. J Mol Biol (2011) 0.88
Solubility engineering and crystallization of human apolipoprotein D. Protein Sci (2005) 0.88
Extra-domain B in oncofetal fibronectin structurally promotes fibrillar head-to-tail dimerization of extracellular matrix protein. J Biol Chem (2012) 0.87
Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach. Biophys Chem (2002) 0.87
Biomarkers for exposure to estrogenic compounds: gene expression analysis in zebrafish (Danio rerio). Environ Toxicol (2008) 0.86
Functional humanization of an anti-CD30 Fab fragment for the immunotherapy of Hodgkin's lymphoma using an in vitro evolution approach. Protein Eng Des Sel (2005) 0.86
Affilin-novel binding molecules based on human gamma-B-crystallin, an all beta-sheet protein. J Mol Biol (2007) 0.86
The Cell Factory Aspergillus Enters the Big Data Era: Opportunities and Challenges for Optimising Product Formation. Adv Biochem Eng Biotechnol (2015) 0.85
Rational engineering of a fluorescein-binding anticalin for improved ligand affinity. Biol Chem (2005) 0.85
Biochemical analysis of recombinant AlkJ from Pseudomonas putida reveals a membrane-associated, flavin adenine dinucleotide-dependent dehydrogenase suitable for the biosynthetic production of aliphatic aldehydes. Appl Environ Microbiol (2014) 0.84
The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization. Biol Chem (2004) 0.84
The oxidative burst reaction in mammalian cells depends on gravity. Cell Commun Signal (2013) 0.84
Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family. Protein Sci (2011) 0.84
Crystal structure of the ω-aminotransferase from Paracoccus denitrificans and its phylogenetic relationship with other class III aminotransferases that have biotechnological potential. Proteins (2013) 0.84
Endoplasmic reticulum stress response promotes cytotoxic phenotype of CD8αβ+ intraepithelial lymphocytes in a mouse model for Crohn's disease-like ileitis. J Immunol (2012) 0.83
Structure of lumazine protein, an optical transponder of luminescent bacteria. J Mol Biol (2008) 0.83
Molecular genetic analysis of vesicular transport in Aspergillus niger reveals partial conservation of the molecular mechanism of exocytosis in fungi. Microbiology (2013) 0.82
Biochemical characterization of the recombinant human Nogo-A ectodomain. FEBS J (2007) 0.82
A photoswitchable neurotransmitter analogue bound to its receptor. Biochemistry (2013) 0.82
Anticalins as an alternative to antibody technology. Expert Opin Biol Ther (2005) 0.82
Structural basis for the enzymatic formation of the key strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone. J Biol Chem (2013) 0.82
High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface. J Mol Biol (2010) 0.82
Crystallographic analysis and structure-guided engineering of NADPH-dependent Ralstonia sp. alcohol dehydrogenase toward NADH cosubstrate specificity. Biotechnol Bioeng (2013) 0.82
High-affinity recognition of lanthanide(III) chelate complexes by a reprogrammed human lipocalin 2. J Am Chem Soc (2009) 0.81
Functional humanization of an anti-CD16 Fab fragment: obstacles of switching from murine {lambda} to human {lambda} or {kappa} light chains. Protein Eng Des Sel (2008) 0.81
Placental transfer of DDT in mother-infant pairs from Northern Thailand. J Environ Sci Health B (2008) 0.81
Bacterial production and functional characterization of the Fab fragment of the murine IgG1/lambda monoclonal antibody cmHsp70.1, a reagent for tumour diagnostics. Protein Eng Des Sel (2010) 0.81
Deactivation of the E. coli pH stress sensor CadC by cadaverine. J Mol Biol (2012) 0.80
New binding mode to TNF-alpha revealed by ubiquitin-based artificial binding protein. PLoS One (2012) 0.80
Combinatorial design of an Anticalin directed against the extra-domain b for the specific targeting of oncofetal fibronectin. J Mol Biol (2012) 0.80
NMR structure and dynamics of the engineered fluorescein-binding lipocalin FluA reveal rigidification of beta-barrel and variable loops upon enthalpy-driven ligand binding. Biochemistry (2009) 0.79
Epitope mapping of the neuronal growth inhibitor Nogo-A for the Nogo receptor and the cognate monoclonal antibody IN-1 by means of the SPOT technique. J Mol Recognit (2007) 0.79
Single-chain Fv antibody-alkaline phosphatase fusion proteins produced by one-step cloning as rapid detection tools for ELISA. J Immunoassay Immunochem (2002) 0.79
Periplasmic chaperones used to enhance functional secretion of proteins in E. coli. Methods Mol Biol (2011) 0.79
Comparative study of estrogenic potencies of estradiol, tamoxifen, bisphenol-A and resveratrol with two in vitro bioassays. Environ Int (2004) 0.79
β-Barrel scaffolds for the grafting of extracellular loops from G-protein-coupled receptors. Biol Chem (2012) 0.78
Anticalins: the lipocalin family as a novel protein scaffold for the development of next-generation immunotherapies. Expert Rev Clin Immunol (2007) 0.78
Intravenous application of an anticalin dramatically lowers plasma digoxin levels and reduces its toxic effects in rats. Toxicol Appl Pharmacol (2012) 0.77
Resonance assignments for the 21 kDa engineered fluorescein-binding lipocalin FluA. J Biomol NMR (2003) 0.77
Structural and biochemical analyses reveal a monomeric state of the bacterial lipocalin Blc. Acta Crystallogr D Biol Crystallogr (2010) 0.77
A novel method for the N-terminal modification of native proteins. Bioconjug Chem (2004) 0.77
The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture. Methods Mol Biol (2015) 0.77
An expression system for the E. coli fermentation of recombinant antibody Fab fragments from mice and rabbits. J AOAC Int (2010) 0.77
The crystal structure of human α(1)-microglobulin reveals a potential haem-binding site. Biochem J (2012) 0.77
NMR structure of lipoprotein YxeF from Bacillus subtilis reveals a calycin fold and distant homology with the lipocalin Blc from Escherichia coli. PLoS One (2012) 0.77
Design and characterization of a modular membrane protein anchor to functionalize the moss Physcomitrella patens with extracellular catalytic and/or binding activities. ACS Synth Biol (2014) 0.77
DNA vaccination efficiently induces antibodies to Nogo-A and does not exacerbate experimental autoimmune encephalomyelitis. Eur J Pharmacol (2008) 0.77
Detection of irradiation-induced, membrane heat shock protein 70 (Hsp70) in mouse tumors using Hsp70 Fab fragment. Radiother Oncol (2011) 0.76
High molecular mass radioimmunoconjugates are promising for intraperitoneal α-emitter immunotherapy due to prolonged retention in the peritoneum. Nucl Med Biol (2012) 0.76
High contrast tumor imaging with radio-labeled antibody Fab fragments tailored for optimized pharmacokinetics via PASylation. MAbs (2015) 0.76
Novel ubiquitin-derived high affinity binding proteins with tumor targeting properties. J Biol Chem (2014) 0.76
Biosynthesis of a fluorescent protein with extreme pseudo-Stokes shift by introducing a genetically encoded non-natural amino acid outside the fluorophore. J Am Chem Soc (2011) 0.76
Generation of anticalins with specificity for a nonsymmetric phthalic acid ester. Anal Biochem (2002) 0.76
Determination of Acaulospora longula and Glomus subgroup Aa in plant roots from grassland using new primers against the large subunit ribosomal DNA. Mycol Res (2004) 0.75
A system for repertoire cloning and phage display of murine and leporid antibody fragments. J AOAC Int (2010) 0.75
Derivatization of antibody Fab fragments: a designer enzyme for native protein modification. Chembiochem (2014) 0.75
PAS-cal: a generic recombinant peptide calibration standard for mass spectrometry. J Am Soc Mass Spectrom (2014) 0.75