Published in Biotechnol Bioeng on July 01, 2013
Structure-based conversion of the coenzyme requirement of a short-chain dehydrogenase/reductase involved in bacterial alginate metabolism. J Biol Chem (2014) 0.86
High-Throughput Screening of Coenzyme Preference Change of Thermophilic 6-Phosphogluconate Dehydrogenase from NADP(+) to NAD(.). Sci Rep (2016) 0.77
Substitutions at the cofactor phosphate-binding site of a clostridial alcohol dehydrogenase lead to unexpected changes in substrate specificity. Protein Eng Des Sel (2015) 0.75
Coenzyme Engineering of a Hyperthermophilic 6-Phosphogluconate Dehydrogenase from NADP(+) to NAD(+) with Its Application to Biobatteries. Sci Rep (2016) 0.75
Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism. Science (2006) 4.21
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nat Protoc (2007) 3.23
Crucial role for human Toll-like receptor 4 in the development of contact allergy to nickel. Nat Immunol (2010) 3.15
Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome. Science (2009) 3.00
Engineered protein scaffolds as next-generation antibody therapeutics. Curr Opin Chem Biol (2009) 2.41
Interferon-gamma regulates intestinal epithelial homeostasis through converging beta-catenin signaling pathways. Immunity (2010) 2.39
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods (2007) 2.04
Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution. Proc Natl Acad Sci U S A (2010) 1.99
Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome. Proc Natl Acad Sci U S A (2010) 1.68
alpha-Helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel. Nat Struct Mol Biol (2010) 1.68
Structure of the no-go mRNA decay complex Dom34-Hbs1 bound to a stalled 80S ribosome. Nat Struct Mol Biol (2011) 1.57
Structure of antibody F425-B4e8 in complex with a V3 peptide reveals a new binding mode for HIV-1 neutralization. J Mol Biol (2007) 1.42
The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands. J Biol Chem (2004) 1.30
Structural insights into the antigenicity of myelin oligodendrocyte glycoprotein. Proc Natl Acad Sci U S A (2003) 1.27
The crystal structure of the bacteriophage PSA endolysin reveals a unique fold responsible for specific recognition of Listeria cell walls. J Mol Biol (2006) 1.26
Binding proteins from alternative scaffolds. J Immunol Methods (2004) 1.26
Temperature and pressure dependence of protein stability: the engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram. Proc Natl Acad Sci U S A (2008) 1.22
Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody. Proc Natl Acad Sci U S A (2010) 1.21
Comparative ligand-binding analysis of ten human lipocalins. Biochim Biophys Acta (2006) 1.21
Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site. Protein Sci (2002) 1.20
A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Protein Eng Des Sel (2006) 1.16
Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture. Nat Struct Mol Biol (2004) 1.13
Crystal structure of lumazine synthase from Mycobacterium tuberculosis as a target for rational drug design: binding mode of a new class of purinetrione inhibitors. Biochemistry (2005) 1.12
Structural insight into the dual ligand specificity and mode of high density lipoprotein association of apolipoprotein D. J Biol Chem (2007) 1.11
A new crystal form of human tear lipocalin reveals high flexibility in the loop region and induced fit in the ligand cavity. Acta Crystallogr D Biol Crystallogr (2009) 1.10
The AcrB efflux pump: conformational cycling and peristalsis lead to multidrug resistance. Curr Drug Targets (2008) 1.09
Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells. Mol Biol Cell (2009) 1.08
X-ray structures of the maltose-maltodextrin-binding protein of the thermoacidophilic bacterium Alicyclobacillus acidocaldarius provide insight into acid stability of proteins. J Mol Biol (2004) 1.06
The 1.8-A crystal structure of alpha1-acid glycoprotein (Orosomucoid) solved by UV RIP reveals the broad drug-binding activity of this human plasma lipocalin. J Mol Biol (2008) 1.04
The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site. Mol Immunol (2007) 1.02
A structure-based model of the reaction catalyzed by lumazine synthase from Aquifex aeolicus. J Mol Biol (2003) 1.02
Structural analysis of the L-alanoyl-D-glutamate endopeptidase domain of Listeria bacteriophage endolysin Ply500 reveals a new member of the LAS peptidase family. Acta Crystallogr D Biol Crystallogr (2008) 1.01
Lipocalins in drug discovery: from natural ligand-binding proteins to "anticalins". Drug Discov Today (2005) 0.99
Novel serial positive enrichment technology enables clinical multiparameter cell sorting. PLoS One (2012) 0.98
Neutrophil gelatinase-associated lipocalin expresses antimicrobial activity by interfering with L-norepinephrine-mediated bacterial iron acquisition. Antimicrob Agents Chemother (2010) 0.97
Structural mechanism of specific ligand recognition by a lipocalin tailored for the complexation of digoxigenin. J Mol Biol (2003) 0.97
PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins. Protein Eng Des Sel (2013) 0.97
Alternative binding proteins get mature: rivalling antibodies. FEBS J (2008) 0.97
Crystallographic analysis of an "anticalin" with tailored specificity for fluorescein reveals high structural plasticity of the lipocalin loop region. Proteins (2003) 0.96
Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: effects on biophysical properties and prolonged plasma half-life. Protein Eng Des Sel (2007) 0.96
Identification of potential substrate proteins for the periplasmic Escherichia coli chaperone Skp. Proteomics (2008) 0.95
Comparative analysis reveals selective recognition of glycans by the dendritic cell receptors DC-SIGN and Langerin. Protein Eng Des Sel (2011) 0.95
Enzyme catalysis via control of activation entropy: site-directed mutagenesis of 6,7-dimethyl-8-ribityllumazine synthase. J Mol Biol (2003) 0.94
Crystallographic analysis of AcrB. FEBS Lett (2004) 0.94
New insights into the signaling mechanism of the pH-responsive, membrane-integrated transcriptional activator CadC of Escherichia coli. J Biol Chem (2011) 0.93
Anticalins small engineered binding proteins based on the lipocalin scaffold. Methods Enzymol (2012) 0.92
The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops. Proteins (2008) 0.90
Construction of an artificial receptor protein ("anticalin") based on the human apolipoprotein D. Chembiochem (2004) 0.90
Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism. Acta Crystallogr Sect F Struct Biol Cryst Commun (2009) 0.89
Multiple assembly states of lumazine synthase: a model relating catalytic function and molecular assembly. J Mol Biol (2006) 0.88
A disulfide-free single-domain V(L) intrabody with blocking activity towards huntingtin reveals a novel mode of epitope recognition. J Mol Biol (2011) 0.88
Solubility engineering and crystallization of human apolipoprotein D. Protein Sci (2005) 0.88
Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach. Biophys Chem (2002) 0.87
Extra-domain B in oncofetal fibronectin structurally promotes fibrillar head-to-tail dimerization of extracellular matrix protein. J Biol Chem (2012) 0.87
Functional humanization of an anti-CD30 Fab fragment for the immunotherapy of Hodgkin's lymphoma using an in vitro evolution approach. Protein Eng Des Sel (2005) 0.86
Rational engineering of a fluorescein-binding anticalin for improved ligand affinity. Biol Chem (2005) 0.85
Biochemical analysis of recombinant AlkJ from Pseudomonas putida reveals a membrane-associated, flavin adenine dinucleotide-dependent dehydrogenase suitable for the biosynthetic production of aliphatic aldehydes. Appl Environ Microbiol (2014) 0.84
The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization. Biol Chem (2004) 0.84
Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family. Protein Sci (2011) 0.84
Crystal structure of the ω-aminotransferase from Paracoccus denitrificans and its phylogenetic relationship with other class III aminotransferases that have biotechnological potential. Proteins (2013) 0.84
Structure of lumazine protein, an optical transponder of luminescent bacteria. J Mol Biol (2008) 0.83
Endoplasmic reticulum stress response promotes cytotoxic phenotype of CD8αβ+ intraepithelial lymphocytes in a mouse model for Crohn's disease-like ileitis. J Immunol (2012) 0.83
Anticalins as an alternative to antibody technology. Expert Opin Biol Ther (2005) 0.82
Structural basis for the enzymatic formation of the key strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone. J Biol Chem (2013) 0.82
Biochemical characterization of the recombinant human Nogo-A ectodomain. FEBS J (2007) 0.82
High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface. J Mol Biol (2010) 0.82
A photoswitchable neurotransmitter analogue bound to its receptor. Biochemistry (2013) 0.82
Functional humanization of an anti-CD16 Fab fragment: obstacles of switching from murine {lambda} to human {lambda} or {kappa} light chains. Protein Eng Des Sel (2008) 0.81
A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of F(ab) fragments and leads to improved recombinant immunoglobulin stability. Biosens Bioelectron (2002) 0.81
High-affinity recognition of lanthanide(III) chelate complexes by a reprogrammed human lipocalin 2. J Am Chem Soc (2009) 0.81
Bacterial production and functional characterization of the Fab fragment of the murine IgG1/lambda monoclonal antibody cmHsp70.1, a reagent for tumour diagnostics. Protein Eng Des Sel (2010) 0.81
Combinatorial design of an Anticalin directed against the extra-domain b for the specific targeting of oncofetal fibronectin. J Mol Biol (2012) 0.80
Virtual screening, selection and development of a benzindolone structural scaffold for inhibition of lumazine synthase. Bioorg Med Chem (2010) 0.80
Deactivation of the E. coli pH stress sensor CadC by cadaverine. J Mol Biol (2012) 0.80
NMR structure and dynamics of the engineered fluorescein-binding lipocalin FluA reveal rigidification of beta-barrel and variable loops upon enthalpy-driven ligand binding. Biochemistry (2009) 0.79
Periplasmic chaperones used to enhance functional secretion of proteins in E. coli. Methods Mol Biol (2011) 0.79
Epitope mapping of the neuronal growth inhibitor Nogo-A for the Nogo receptor and the cognate monoclonal antibody IN-1 by means of the SPOT technique. J Mol Recognit (2007) 0.79
β-Barrel scaffolds for the grafting of extracellular loops from G-protein-coupled receptors. Biol Chem (2012) 0.78
Anticalins: the lipocalin family as a novel protein scaffold for the development of next-generation immunotherapies. Expert Rev Clin Immunol (2007) 0.78
Molecular insights into γδ T cell costimulation by an anti-JAML antibody. Structure (2011) 0.78
Resonance assignments for the 21 kDa engineered fluorescein-binding lipocalin FluA. J Biomol NMR (2003) 0.77
A novel method for the N-terminal modification of native proteins. Bioconjug Chem (2004) 0.77
Structural and biochemical analyses reveal a monomeric state of the bacterial lipocalin Blc. Acta Crystallogr D Biol Crystallogr (2010) 0.77
Structure of Methanocaldococcus jannaschii nucleoside kinase: an archaeal member of the ribokinase family. Acta Crystallogr D Biol Crystallogr (2006) 0.77
The crystal structure of human α(1)-microglobulin reveals a potential haem-binding site. Biochem J (2012) 0.77
An expression system for the E. coli fermentation of recombinant antibody Fab fragments from mice and rabbits. J AOAC Int (2010) 0.77
The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture. Methods Mol Biol (2015) 0.77
NMR structure of lipoprotein YxeF from Bacillus subtilis reveals a calycin fold and distant homology with the lipocalin Blc from Escherichia coli. PLoS One (2012) 0.77
Design and characterization of a modular membrane protein anchor to functionalize the moss Physcomitrella patens with extracellular catalytic and/or binding activities. ACS Synth Biol (2014) 0.77
Intravenous application of an anticalin dramatically lowers plasma digoxin levels and reduces its toxic effects in rats. Toxicol Appl Pharmacol (2012) 0.77
DNA vaccination efficiently induces antibodies to Nogo-A and does not exacerbate experimental autoimmune encephalomyelitis. Eur J Pharmacol (2008) 0.77
High contrast tumor imaging with radio-labeled antibody Fab fragments tailored for optimized pharmacokinetics via PASylation. MAbs (2015) 0.76
Crystallization and preliminary X-ray analysis of Mlc from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun (2005) 0.76
Detection of irradiation-induced, membrane heat shock protein 70 (Hsp70) in mouse tumors using Hsp70 Fab fragment. Radiother Oncol (2011) 0.76
High molecular mass radioimmunoconjugates are promising for intraperitoneal α-emitter immunotherapy due to prolonged retention in the peritoneum. Nucl Med Biol (2012) 0.76
Generation of anticalins with specificity for a nonsymmetric phthalic acid ester. Anal Biochem (2002) 0.76