Published in Proc Natl Acad Sci U S A on April 07, 2008
Computing the stability diagram of the Trp-cage miniprotein. Proc Natl Acad Sci U S A (2008) 1.45
Unique features of the folding landscape of a repeat protein revealed by pressure perturbation. Biophys J (2010) 0.93
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Why elliptical stability diagrams are not related to protein conformational disorder. Proc Natl Acad Sci U S A (2008) 0.75
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Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold. Proc Natl Acad Sci U S A (1999) 1.34
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Mosaic energy landscapes of liquids and the control of protein conformational dynamics by glass-forming solvents. J Phys Chem B (2005) 1.24
Simulations of the pressure and temperature unfolding of an alpha-helical peptide. Proc Natl Acad Sci U S A (2005) 1.21
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Ultrafast electron transfer in the complex between fluorescein and a cognate engineered lipocalin protein, a so-called anticalin. Biochemistry (2002) 1.12
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Exploring the temperature-pressure configurational landscape of biomolecules: from lipid membranes to proteins. Philos Trans A Math Phys Eng Sci (2005) 0.99
Crystallographic analysis of an "anticalin" with tailored specificity for fluorescein reveals high structural plasticity of the lipocalin loop region. Proteins (2003) 0.96
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Stability diagram and unfolding of a modified cytochrome c: what happens in the transformation regime? Biophys J (2002) 0.86
Rational engineering of a fluorescein-binding anticalin for improved ligand affinity. Biol Chem (2005) 0.85
Heteropolymer collapse theory for protein folding in the pressure-temperature plane. Biophys J (2006) 0.84
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nat Protoc (2007) 3.23
Crucial role for human Toll-like receptor 4 in the development of contact allergy to nickel. Nat Immunol (2010) 3.15
Engineered protein scaffolds as next-generation antibody therapeutics. Curr Opin Chem Biol (2009) 2.41
Interferon-gamma regulates intestinal epithelial homeostasis through converging beta-catenin signaling pathways. Immunity (2010) 2.39
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods (2007) 2.04
Protein phase diagrams: the physics behind their elliptic shape. J Chem Phys (2004) 1.77
The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands. J Biol Chem (2004) 1.30
Structural insights into the antigenicity of myelin oligodendrocyte glycoprotein. Proc Natl Acad Sci U S A (2003) 1.27
The crystal structure of the bacteriophage PSA endolysin reveals a unique fold responsible for specific recognition of Listeria cell walls. J Mol Biol (2006) 1.26
Binding proteins from alternative scaffolds. J Immunol Methods (2004) 1.26
Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody. Proc Natl Acad Sci U S A (2010) 1.21
Comparative ligand-binding analysis of ten human lipocalins. Biochim Biophys Acta (2006) 1.21
Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site. Protein Sci (2002) 1.20
A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Protein Eng Des Sel (2006) 1.16
Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture. Nat Struct Mol Biol (2004) 1.13
Structural insight into the dual ligand specificity and mode of high density lipoprotein association of apolipoprotein D. J Biol Chem (2007) 1.11
A new crystal form of human tear lipocalin reveals high flexibility in the loop region and induced fit in the ligand cavity. Acta Crystallogr D Biol Crystallogr (2009) 1.10
Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells. Mol Biol Cell (2009) 1.08
The 1.8-A crystal structure of alpha1-acid glycoprotein (Orosomucoid) solved by UV RIP reveals the broad drug-binding activity of this human plasma lipocalin. J Mol Biol (2008) 1.04
The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site. Mol Immunol (2007) 1.02
Structural analysis of the L-alanoyl-D-glutamate endopeptidase domain of Listeria bacteriophage endolysin Ply500 reveals a new member of the LAS peptidase family. Acta Crystallogr D Biol Crystallogr (2008) 1.01
Lipocalins in drug discovery: from natural ligand-binding proteins to "anticalins". Drug Discov Today (2005) 0.99
Novel serial positive enrichment technology enables clinical multiparameter cell sorting. PLoS One (2012) 0.98
Neutrophil gelatinase-associated lipocalin expresses antimicrobial activity by interfering with L-norepinephrine-mediated bacterial iron acquisition. Antimicrob Agents Chemother (2010) 0.97
Alternative binding proteins get mature: rivalling antibodies. FEBS J (2008) 0.97
PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins. Protein Eng Des Sel (2013) 0.97
Structural mechanism of specific ligand recognition by a lipocalin tailored for the complexation of digoxigenin. J Mol Biol (2003) 0.97
Crystallographic analysis of an "anticalin" with tailored specificity for fluorescein reveals high structural plasticity of the lipocalin loop region. Proteins (2003) 0.96
Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: effects on biophysical properties and prolonged plasma half-life. Protein Eng Des Sel (2007) 0.96
Labeling proteins via hole burning of their aromatic amino acids: pressure tuning spectroscopy of BPTI. Biophys J (2002) 0.95
Comparative analysis reveals selective recognition of glycans by the dendritic cell receptors DC-SIGN and Langerin. Protein Eng Des Sel (2011) 0.95
Identification of potential substrate proteins for the periplasmic Escherichia coli chaperone Skp. Proteomics (2008) 0.95
New insights into the signaling mechanism of the pH-responsive, membrane-integrated transcriptional activator CadC of Escherichia coli. J Biol Chem (2011) 0.93
Anticalins small engineered binding proteins based on the lipocalin scaffold. Methods Enzymol (2012) 0.92
The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops. Proteins (2008) 0.90
Construction of an artificial receptor protein ("anticalin") based on the human apolipoprotein D. Chembiochem (2004) 0.90
Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism. Acta Crystallogr Sect F Struct Biol Cryst Commun (2009) 0.89
Stability of proteins: temperature, pressure and the role of the solvent. Biochim Biophys Acta (2005) 0.89
Solubility engineering and crystallization of human apolipoprotein D. Protein Sci (2005) 0.88
A disulfide-free single-domain V(L) intrabody with blocking activity towards huntingtin reveals a novel mode of epitope recognition. J Mol Biol (2011) 0.88
Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach. Biophys Chem (2002) 0.87
Extra-domain B in oncofetal fibronectin structurally promotes fibrillar head-to-tail dimerization of extracellular matrix protein. J Biol Chem (2012) 0.87
Functional humanization of an anti-CD30 Fab fragment for the immunotherapy of Hodgkin's lymphoma using an in vitro evolution approach. Protein Eng Des Sel (2005) 0.86
Stability diagram and unfolding of a modified cytochrome c: what happens in the transformation regime? Biophys J (2002) 0.86
Rational engineering of a fluorescein-binding anticalin for improved ligand affinity. Biol Chem (2005) 0.85
Biochemical analysis of recombinant AlkJ from Pseudomonas putida reveals a membrane-associated, flavin adenine dinucleotide-dependent dehydrogenase suitable for the biosynthetic production of aliphatic aldehydes. Appl Environ Microbiol (2014) 0.84
Crystal structure of the ω-aminotransferase from Paracoccus denitrificans and its phylogenetic relationship with other class III aminotransferases that have biotechnological potential. Proteins (2013) 0.84
Size distribution of pressure-decomposed casein micelles studied by dynamic light scattering and AFM. Eur Biophys J (2006) 0.84
The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization. Biol Chem (2004) 0.84
Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family. Protein Sci (2011) 0.84
Endoplasmic reticulum stress response promotes cytotoxic phenotype of CD8αβ+ intraepithelial lymphocytes in a mouse model for Crohn's disease-like ileitis. J Immunol (2012) 0.83
Structure of lumazine protein, an optical transponder of luminescent bacteria. J Mol Biol (2008) 0.83
Crystallographic analysis and structure-guided engineering of NADPH-dependent Ralstonia sp. alcohol dehydrogenase toward NADH cosubstrate specificity. Biotechnol Bioeng (2013) 0.82
A photoswitchable neurotransmitter analogue bound to its receptor. Biochemistry (2013) 0.82
Anticalins as an alternative to antibody technology. Expert Opin Biol Ther (2005) 0.82
Structural basis for the enzymatic formation of the key strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone. J Biol Chem (2013) 0.82
Biochemical characterization of the recombinant human Nogo-A ectodomain. FEBS J (2007) 0.82
High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface. J Mol Biol (2010) 0.82
A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of F(ab) fragments and leads to improved recombinant immunoglobulin stability. Biosens Bioelectron (2002) 0.81
Functional humanization of an anti-CD16 Fab fragment: obstacles of switching from murine {lambda} to human {lambda} or {kappa} light chains. Protein Eng Des Sel (2008) 0.81
High-affinity recognition of lanthanide(III) chelate complexes by a reprogrammed human lipocalin 2. J Am Chem Soc (2009) 0.81
Bacterial production and functional characterization of the Fab fragment of the murine IgG1/lambda monoclonal antibody cmHsp70.1, a reagent for tumour diagnostics. Protein Eng Des Sel (2010) 0.81
Combinatorial design of an Anticalin directed against the extra-domain b for the specific targeting of oncofetal fibronectin. J Mol Biol (2012) 0.80
Deactivation of the E. coli pH stress sensor CadC by cadaverine. J Mol Biol (2012) 0.80
NMR structure and dynamics of the engineered fluorescein-binding lipocalin FluA reveal rigidification of beta-barrel and variable loops upon enthalpy-driven ligand binding. Biochemistry (2009) 0.79
Periplasmic chaperones used to enhance functional secretion of proteins in E. coli. Methods Mol Biol (2011) 0.79
Protein elasticity determined by pressure tuning of the tyrosine residue of ubiquitin. J Chem Phys (2007) 0.79
Epitope mapping of the neuronal growth inhibitor Nogo-A for the Nogo receptor and the cognate monoclonal antibody IN-1 by means of the SPOT technique. J Mol Recognit (2007) 0.79
β-Barrel scaffolds for the grafting of extracellular loops from G-protein-coupled receptors. Biol Chem (2012) 0.78
Anticalins: the lipocalin family as a novel protein scaffold for the development of next-generation immunotherapies. Expert Rev Clin Immunol (2007) 0.78
An expression system for the E. coli fermentation of recombinant antibody Fab fragments from mice and rabbits. J AOAC Int (2010) 0.77
A novel method for the N-terminal modification of native proteins. Bioconjug Chem (2004) 0.77
Resonance assignments for the 21 kDa engineered fluorescein-binding lipocalin FluA. J Biomol NMR (2003) 0.77
The crystal structure of human α(1)-microglobulin reveals a potential haem-binding site. Biochem J (2012) 0.77
Structural and biochemical analyses reveal a monomeric state of the bacterial lipocalin Blc. Acta Crystallogr D Biol Crystallogr (2010) 0.77
Intravenous application of an anticalin dramatically lowers plasma digoxin levels and reduces its toxic effects in rats. Toxicol Appl Pharmacol (2012) 0.77
The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture. Methods Mol Biol (2015) 0.77
Local compressibilities of proteins: comparison of optical experiments and simulations for horse heart cytochrome-c. Biophys J (2005) 0.77
NMR structure of lipoprotein YxeF from Bacillus subtilis reveals a calycin fold and distant homology with the lipocalin Blc from Escherichia coli. PLoS One (2012) 0.77
Design and characterization of a modular membrane protein anchor to functionalize the moss Physcomitrella patens with extracellular catalytic and/or binding activities. ACS Synth Biol (2014) 0.77
DNA vaccination efficiently induces antibodies to Nogo-A and does not exacerbate experimental autoimmune encephalomyelitis. Eur J Pharmacol (2008) 0.77
Generation of anticalins with specificity for a nonsymmetric phthalic acid ester. Anal Biochem (2002) 0.76
Biosynthesis of a fluorescent protein with extreme pseudo-Stokes shift by introducing a genetically encoded non-natural amino acid outside the fluorophore. J Am Chem Soc (2011) 0.76
High contrast tumor imaging with radio-labeled antibody Fab fragments tailored for optimized pharmacokinetics via PASylation. MAbs (2015) 0.76
Detection of irradiation-induced, membrane heat shock protein 70 (Hsp70) in mouse tumors using Hsp70 Fab fragment. Radiother Oncol (2011) 0.76
High molecular mass radioimmunoconjugates are promising for intraperitoneal α-emitter immunotherapy due to prolonged retention in the peritoneum. Nucl Med Biol (2012) 0.76
Derivatization of antibody Fab fragments: a designer enzyme for native protein modification. Chembiochem (2014) 0.75
A system for repertoire cloning and phage display of murine and leporid antibody fragments. J AOAC Int (2010) 0.75
PAS-cal: a generic recombinant peptide calibration standard for mass spectrometry. J Am Soc Mass Spectrom (2014) 0.75
Engineering of an orthogonal aminoacyl-tRNA synthetase for efficient incorporation of the non-natural amino acid O-methyl-L-tyrosine using fluorescence-based bacterial cell sorting. J Mol Biol (2010) 0.75
A HLA-Cw6 specific single-chain antibody fragment (scFv) recognizing a natural killer cell receptor epitope. Mol Immunol (2005) 0.75
Terrylene in hexadecane revisited: a hole burning study. J Chem Phys (2007) 0.75
Anticalins directed against vascular endothelial growth factor receptor 3 (VEGFR-3) with picomolar affinities show potential for medical therapy and in vivo imaging. Biol Chem (2017) 0.75
Local compressibilities in insulin as determined from pressure tuning hole burning experiments and MD simulations. Phys Chem Chem Phys (2005) 0.75
Investigation of spectral diffusion in ribonuclease by photolabeling of intrinsic aromatic amino acids. J Chem Phys (2006) 0.75
Hole burning spectroscopy of ribonuclease A. Phys Chem Chem Phys (2006) 0.75
Aging dynamics in globular proteins: summary and analysis of experimental results and simulation by a modified trap model. Eur Biophys J (2003) 0.75
PAS-cal: A repetitive peptide sequence calibration standard for MALDI mass spectrometry. Proteomics (2014) 0.75
Thermal-cycling-induced spectral diffusion and thermal barriers in anisole-doped cyclohexane, an unusual multiphase host-guest system. J Phys Chem B (2006) 0.75