Published in Proteomics on December 01, 2008
SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm. Microb Cell Fact (2012) 2.10
The Bam machine: a molecular cooper. Biochim Biophys Acta (2011) 1.92
Dissecting the Escherichia coli periplasmic chaperone network using differential proteomics. Proteomics (2012) 1.07
Conformation and dynamics of the periplasmic membrane-protein-chaperone complexes OmpX-Skp and tOmpA-Skp. Nat Struct Mol Biol (2013) 1.00
Role for Skp in LptD assembly in Escherichia coli. J Bacteriol (2013) 0.97
Impact of holdase chaperones Skp and SurA on the folding of β-barrel outer-membrane proteins. Nat Struct Mol Biol (2015) 0.90
Broad host range vectors for expression of proteins with (Twin-) Strep-tag, His-tag and engineered, export optimized yellow fluorescent protein. Microb Cell Fact (2013) 0.86
The Skp chaperone helps fold soluble proteins in vitro by inhibiting aggregation. Biochemistry (2012) 0.86
Protein folding in the cell envelope of Escherichia coli. Nat Microbiol (2016) 0.83
Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli. Microb Cell Fact (2009) 0.82
Protective Enterotoxigenic Escherichia coli Antigens in a Murine Intranasal Challenge Model. PLoS Negl Trop Dis (2015) 0.81
Revisiting the interaction between the chaperone Skp and lipopolysaccharide. Biophys J (2015) 0.79
From Chaperones to the Membrane with a BAM! Trends Biochem Sci (2016) 0.78
Deuterium Labeling Together with Contrast Variation Small-Angle Neutron Scattering Suggests How Skp Captures and Releases Unfolded Outer Membrane Proteins. Methods Enzymol (2015) 0.77
Disentangling the influence of earthworms in sugarcane rhizosphere. Sci Rep (2016) 0.75
Skp is a multivalent chaperone of outer-membrane proteins. Nat Struct Mol Biol (2016) 0.75
Assembly of Outer Membrane β-Barrel Proteins: the Bam Complex. Ecosal Plus (2011) 0.75
Guidelines for the next 10 years of proteomics. Proteomics (2006) 3.61
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nat Protoc (2007) 3.23
Crucial role for human Toll-like receptor 4 in the development of contact allergy to nickel. Nat Immunol (2010) 3.15
Engineered protein scaffolds as next-generation antibody therapeutics. Curr Opin Chem Biol (2009) 2.41
Interferon-gamma regulates intestinal epithelial homeostasis through converging beta-catenin signaling pathways. Immunity (2010) 2.39
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods (2007) 2.04
Identification of quorum-sensing regulated proteins in the opportunistic pathogen Pseudomonas aeruginosa by proteomics. Environ Microbiol (2003) 1.33
The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands. J Biol Chem (2004) 1.30
Structural insights into the antigenicity of myelin oligodendrocyte glycoprotein. Proc Natl Acad Sci U S A (2003) 1.27
The crystal structure of the bacteriophage PSA endolysin reveals a unique fold responsible for specific recognition of Listeria cell walls. J Mol Biol (2006) 1.26
Binding proteins from alternative scaffolds. J Immunol Methods (2004) 1.26
Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants. Proc Natl Acad Sci U S A (2007) 1.25
Temperature and pressure dependence of protein stability: the engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram. Proc Natl Acad Sci U S A (2008) 1.22
Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody. Proc Natl Acad Sci U S A (2010) 1.21
Comparative ligand-binding analysis of ten human lipocalins. Biochim Biophys Acta (2006) 1.21
Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site. Protein Sci (2002) 1.20
2-DE with IPGs. Electrophoresis (2009) 1.20
A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Protein Eng Des Sel (2006) 1.16
Analysis of the quorum-sensing regulon of the opportunistic pathogen Burkholderia cepacia H111 by proteomics. Electrophoresis (2003) 1.16
Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture. Nat Struct Mol Biol (2004) 1.13
Structural insight into the dual ligand specificity and mode of high density lipoprotein association of apolipoprotein D. J Biol Chem (2007) 1.11
A new crystal form of human tear lipocalin reveals high flexibility in the loop region and induced fit in the ligand cavity. Acta Crystallogr D Biol Crystallogr (2009) 1.10
Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells. Mol Biol Cell (2009) 1.08
The 1.8-A crystal structure of alpha1-acid glycoprotein (Orosomucoid) solved by UV RIP reveals the broad drug-binding activity of this human plasma lipocalin. J Mol Biol (2008) 1.04
Protein detection and quantitation technologies for gel-based proteome analysis. Methods Mol Biol (2009) 1.02
The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site. Mol Immunol (2007) 1.02
Structural analysis of the L-alanoyl-D-glutamate endopeptidase domain of Listeria bacteriophage endolysin Ply500 reveals a new member of the LAS peptidase family. Acta Crystallogr D Biol Crystallogr (2008) 1.01
Lipocalins in drug discovery: from natural ligand-binding proteins to "anticalins". Drug Discov Today (2005) 0.99
Novel serial positive enrichment technology enables clinical multiparameter cell sorting. PLoS One (2012) 0.98
Neutrophil gelatinase-associated lipocalin expresses antimicrobial activity by interfering with L-norepinephrine-mediated bacterial iron acquisition. Antimicrob Agents Chemother (2010) 0.97
PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins. Protein Eng Des Sel (2013) 0.97
Alternative binding proteins get mature: rivalling antibodies. FEBS J (2008) 0.97
Structural mechanism of specific ligand recognition by a lipocalin tailored for the complexation of digoxigenin. J Mol Biol (2003) 0.97
Crystallographic analysis of an "anticalin" with tailored specificity for fluorescein reveals high structural plasticity of the lipocalin loop region. Proteins (2003) 0.96
Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: effects on biophysical properties and prolonged plasma half-life. Protein Eng Des Sel (2007) 0.96
Comparative analysis reveals selective recognition of glycans by the dendritic cell receptors DC-SIGN and Langerin. Protein Eng Des Sel (2011) 0.95
New insights into the signaling mechanism of the pH-responsive, membrane-integrated transcriptional activator CadC of Escherichia coli. J Biol Chem (2011) 0.93
Anticalins small engineered binding proteins based on the lipocalin scaffold. Methods Enzymol (2012) 0.92
The UV-B stimulon of the terrestrial cyanobacterium Nostoc commune comprises early shock proteins and late acclimation proteins. Mol Microbiol (2002) 0.92
The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops. Proteins (2008) 0.90
Construction of an artificial receptor protein ("anticalin") based on the human apolipoprotein D. Chembiochem (2004) 0.90
Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism. Acta Crystallogr Sect F Struct Biol Cryst Commun (2009) 0.89
Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis. Proteomics (2008) 0.89
Solubility engineering and crystallization of human apolipoprotein D. Protein Sci (2005) 0.88
A disulfide-free single-domain V(L) intrabody with blocking activity towards huntingtin reveals a novel mode of epitope recognition. J Mol Biol (2011) 0.88
Biofilm formation of Pseudomonas putida IsoF: the role of quorum sensing as assessed by proteomics. Syst Appl Microbiol (2005) 0.87
Extra-domain B in oncofetal fibronectin structurally promotes fibrillar head-to-tail dimerization of extracellular matrix protein. J Biol Chem (2012) 0.87
Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach. Biophys Chem (2002) 0.87
High-resolution two-dimensional electrophoresis. Methods Mol Biol (2009) 0.86
Setting up standards and a reference map for the alkaline proteome of the Gram-positive bacterium Lactococcus lactis. Proteomics (2004) 0.86
Web-based two-dimensional database of Saccharomyces cerevisiae proteins using immobilized pH gradients from pH 6 to pH 12 and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Proteomics (2002) 0.86
Functional humanization of an anti-CD30 Fab fragment for the immunotherapy of Hodgkin's lymphoma using an in vitro evolution approach. Protein Eng Des Sel (2005) 0.86
Rational engineering of a fluorescein-binding anticalin for improved ligand affinity. Biol Chem (2005) 0.85
Two-dimensional electrophoresis in proteomics. Methods Biochem Anal (2011) 0.84
Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family. Protein Sci (2011) 0.84
The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization. Biol Chem (2004) 0.84
Crystal structure of the ω-aminotransferase from Paracoccus denitrificans and its phylogenetic relationship with other class III aminotransferases that have biotechnological potential. Proteins (2013) 0.84
Biochemical analysis of recombinant AlkJ from Pseudomonas putida reveals a membrane-associated, flavin adenine dinucleotide-dependent dehydrogenase suitable for the biosynthetic production of aliphatic aldehydes. Appl Environ Microbiol (2014) 0.84
Endoplasmic reticulum stress response promotes cytotoxic phenotype of CD8αβ+ intraepithelial lymphocytes in a mouse model for Crohn's disease-like ileitis. J Immunol (2012) 0.83
Sample solublization buffers for two-dimensional electrophoresis. Methods Mol Biol (2008) 0.83
Structure of lumazine protein, an optical transponder of luminescent bacteria. J Mol Biol (2008) 0.83
High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface. J Mol Biol (2010) 0.82
Crystallographic analysis and structure-guided engineering of NADPH-dependent Ralstonia sp. alcohol dehydrogenase toward NADH cosubstrate specificity. Biotechnol Bioeng (2013) 0.82
Anticalins as an alternative to antibody technology. Expert Opin Biol Ther (2005) 0.82
Biochemical characterization of the recombinant human Nogo-A ectodomain. FEBS J (2007) 0.82
A photoswitchable neurotransmitter analogue bound to its receptor. Biochemistry (2013) 0.82
Structural basis for the enzymatic formation of the key strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone. J Biol Chem (2013) 0.82
Two-dimensional electrophoresis for plant proteomics. Methods Mol Biol (2007) 0.81
High-affinity recognition of lanthanide(III) chelate complexes by a reprogrammed human lipocalin 2. J Am Chem Soc (2009) 0.81
A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of F(ab) fragments and leads to improved recombinant immunoglobulin stability. Biosens Bioelectron (2002) 0.81
Functional humanization of an anti-CD16 Fab fragment: obstacles of switching from murine {lambda} to human {lambda} or {kappa} light chains. Protein Eng Des Sel (2008) 0.81
Bacterial production and functional characterization of the Fab fragment of the murine IgG1/lambda monoclonal antibody cmHsp70.1, a reagent for tumour diagnostics. Protein Eng Des Sel (2010) 0.81
A proteome analysis of Corynebacterium glutamicum after exposure to the herbicide 2,4-dichlorophenoxy acetic acid (2,4-D). Chemosphere (2007) 0.80
Deactivation of the E. coli pH stress sensor CadC by cadaverine. J Mol Biol (2012) 0.80
Proteome analysis of intraclonal diversity of two Pseudomonas aeruginosa TB clone isolates. Proteomics (2004) 0.80
Combinatorial design of an Anticalin directed against the extra-domain b for the specific targeting of oncofetal fibronectin. J Mol Biol (2012) 0.80
NMR structure and dynamics of the engineered fluorescein-binding lipocalin FluA reveal rigidification of beta-barrel and variable loops upon enthalpy-driven ligand binding. Biochemistry (2009) 0.79
A proteome analysis of the cadmium and mercury response in Corynebacterium glutamicum. Proteomics (2008) 0.79
Periplasmic chaperones used to enhance functional secretion of proteins in E. coli. Methods Mol Biol (2011) 0.79
Epitope mapping of the neuronal growth inhibitor Nogo-A for the Nogo receptor and the cognate monoclonal antibody IN-1 by means of the SPOT technique. J Mol Recognit (2007) 0.79
β-Barrel scaffolds for the grafting of extracellular loops from G-protein-coupled receptors. Biol Chem (2012) 0.78
Anticalins: the lipocalin family as a novel protein scaffold for the development of next-generation immunotherapies. Expert Rev Clin Immunol (2007) 0.78
A novel method for the N-terminal modification of native proteins. Bioconjug Chem (2004) 0.77
Design and characterization of a modular membrane protein anchor to functionalize the moss Physcomitrella patens with extracellular catalytic and/or binding activities. ACS Synth Biol (2014) 0.77
Resonance assignments for the 21 kDa engineered fluorescein-binding lipocalin FluA. J Biomol NMR (2003) 0.77
An expression system for the E. coli fermentation of recombinant antibody Fab fragments from mice and rabbits. J AOAC Int (2010) 0.77
The crystal structure of human α(1)-microglobulin reveals a potential haem-binding site. Biochem J (2012) 0.77
Structural and biochemical analyses reveal a monomeric state of the bacterial lipocalin Blc. Acta Crystallogr D Biol Crystallogr (2010) 0.77
Intravenous application of an anticalin dramatically lowers plasma digoxin levels and reduces its toxic effects in rats. Toxicol Appl Pharmacol (2012) 0.77
The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture. Methods Mol Biol (2015) 0.77
NMR structure of lipoprotein YxeF from Bacillus subtilis reveals a calycin fold and distant homology with the lipocalin Blc from Escherichia coli. PLoS One (2012) 0.77
DNA vaccination efficiently induces antibodies to Nogo-A and does not exacerbate experimental autoimmune encephalomyelitis. Eur J Pharmacol (2008) 0.77
Corynebacterium glutamicum as an indicator for environmental cobalt and silver stress--a proteome analysis. J Environ Sci Health B (2010) 0.76
High pressure effects step-wise altered protein expression in Lactobacillus sanfranciscensis. Proteomics (2002) 0.76
Detection of irradiation-induced, membrane heat shock protein 70 (Hsp70) in mouse tumors using Hsp70 Fab fragment. Radiother Oncol (2011) 0.76
Biosynthesis of a fluorescent protein with extreme pseudo-Stokes shift by introducing a genetically encoded non-natural amino acid outside the fluorophore. J Am Chem Soc (2011) 0.76
High contrast tumor imaging with radio-labeled antibody Fab fragments tailored for optimized pharmacokinetics via PASylation. MAbs (2015) 0.76