Published in Proc Natl Acad Sci U S A on June 24, 2004
Structure and mechanism of the rebeccamycin sugar 4'-O-methyltransferase RebM. J Biol Chem (2008) 1.12
The crystal structure of the novobiocin biosynthetic enzyme NovP: the first representative structure for the TylF O-methyltransferase superfamily. J Mol Biol (2009) 0.88
Discovery, characterization and comparison of inhibitors of Bacillus anthracis and Staphylococcus aureus replicative DNA helicases. Bioorg Med Chem (2009) 0.87
Monitoring enzyme catalysis in the multimeric state: direct observation of Arthrobacter 4-hydroxybenzoyl-coenzyme A thioesterase catalytic complexes using time-resolved electrospray ionization mass spectrometry. Anal Biochem (2009) 0.80
Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc Natl Acad Sci U S A (1976) 12.30
Crystal structure of an N-terminal fragment of the DNA gyrase B protein. Nature (1991) 3.93
Effects of novobiocin, coumermycin A1, clorobiocin, and their analogs on Escherichia coli DNA gyrase and bacterial growth. Antimicrob Agents Chemother (1982) 3.15
The nature of inhibition of DNA gyrase by the coumarins and the cyclothialidines revealed by X-ray crystallography. EMBO J (1996) 2.60
DNA gyrase as a drug target. Trends Microbiol (1997) 2.55
The high-resolution crystal structure of a 24-kDa gyrase B fragment from E. coli complexed with one of the most potent coumarin inhibitors, clorobiocin. Proteins (1997) 1.40
Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891. Antimicrob Agents Chemother (2000) 1.40
Probing the binding of coumarins and cyclothialidines to DNA gyrase. Biochemistry (1999) 1.32
The interaction of coumarin antibiotics with fragments of DNA gyrase B protein. Biochemistry (1996) 1.27
Methicillin-resistant Staphylococcus aureus: the persistent resistant nosocomial pathogen. Curr Clin Top Infect Dis (1994) 1.14
The effect of bacterial DNA gyrase inhibitors on DNA synthesis in mammalian mitochondria. Biochim Biophys Acta (1983) 1.09
Cloning, overexpression, and purification of novobiocic acid synthetase from Streptomyces spheroides NCIMB 11891. J Biol Chem (2000) 1.06
Multiplex inhibitor screening and kinetic constant determinations for yeast hexokinase using mass spectrometry based assays. J Am Soc Mass Spectrom (2003) 1.06
Novobiocin inhibition of DNA excision repair may occur through effects on mitochondrial structure and ATP metabolism, not on repair topoisomerases. Carcinogenesis (1985) 1.02
Three-dimensional model of Escherichia coli gyrase B subunit crystallized in two-dimensions on novobiocin-linked phospholipid films. J Mol Biol (1994) 1.01
Effect of novobiocin on DNA-dependent DNA polymerases from developing rat brain. Biochim Biophys Acta (1974) 1.00
Synthesis and in vitro evaluation of novel highly potent coumarin inhibitors of gyrase B. Bioorg Med Chem Lett (1999) 1.00
Characterization of NovP and NovN: completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring. Angew Chem Int Ed Engl (2004) 1.00
Kinetic analysis of NodST sulfotransferase using an electrospray ionization mass spectrometry assay. Biochemistry (2002) 0.98
The additional 165 amino acids in the B protein of Escherichia coli DNA gyrase have an important role in DNA binding. J Biol Chem (2000) 0.97
Investigation of enzyme kinetics using quench-flow techniques with MALDI-TOF mass spectrometry. Anal Chem (2000) 0.97
Observation of a hybrid random ping-pong mechanism of catalysis for NodST: a mass spectrometry approach. Protein Sci (2004) 0.94
Structure-activity relationship in two series of aminoalkyl substituted coumarin inhibitors of gyrase B. Bioorg Med Chem Lett (1999) 0.93
Use of a multiple-enzyme/multiple-reagent assay system to quantify activity levels in samples containing mixtures of matrix metalloproteinases. Biochemistry (2004) 0.92
Initial characterization of novobiocic acid noviosyl transferase activity of NovM in biosynthesis of the antibiotic novobiocin. Biochemistry (2003) 0.90
Determination of enzyme/substrate specificity constants using a multiple substrate ESI-MS assay. J Am Soc Mass Spectrom (2004) 0.89
Coumarins as inhibitors of bacterial DNA gyrase. J Antibiot (Tokyo) (1988) 0.85
A strategy for the determination of enzyme kinetics using electrospray ionization with an ion trap mass spectrometer. Anal Chem (2001) 0.85
Kinetic measurements of phosphoglucomutase by direct analysis of glucose-1-phosphate and glucose-6-phosphate using ion/molecule reactions and Fourier transform ion cyclotron resonance mass spectrometry. Anal Biochem (2004) 0.83
Synthesis and biological evaluation of coumarincarboxylic acids as inhibitors of gyrase B. L-rhamnose as an effective substitute for L-noviose. Bioorg Med Chem Lett (1999) 0.83
Kinetic monitoring of enzymatic reactions in real time by quantitative high-performance liquid chromatography-mass spectrometry. Anal Biochem (1995) 0.79
Assembly-line enzymology for polyketide and nonribosomal Peptide antibiotics: logic, machinery, and mechanisms. Chem Rev (2006) 7.69
Antibiotics for emerging pathogens. Science (2009) 7.39
Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature. Nat Prod Rep (2013) 5.38
Genetics and assembly line enzymology of siderophore biosynthesis in bacteria. Microbiol Mol Biol Rev (2002) 5.12
Non-heme Fe(IV)-oxo intermediates. Acc Chem Res (2007) 3.55
Two interconverting Fe(IV) intermediates in aliphatic chlorination by the halogenase CytC3. Nat Chem Biol (2007) 3.33
Cryptic chlorination by a non-haem iron enzyme during cyclopropyl amino acid biosynthesis. Nature (2005) 2.91
New antibiotics from bacterial natural products. Nat Biotechnol (2006) 2.72
The evolution of gene collectives: How natural selection drives chemical innovation. Proc Natl Acad Sci U S A (2008) 2.71
The pathogen-associated iroA gene cluster mediates bacterial evasion of lipocalin 2. Proc Natl Acad Sci U S A (2006) 2.63
MmpL8 is required for sulfolipid-1 biosynthesis and Mycobacterium tuberculosis virulence. Proc Natl Acad Sci U S A (2003) 2.62
BluB cannibalizes flavin to form the lower ligand of vitamin B12. Nature (2007) 2.53
Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis. Nature (2006) 2.52
Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3. Proc Natl Acad Sci U S A (2008) 2.50
How pathogenic bacteria evade mammalian sabotage in the battle for iron. Nat Chem Biol (2006) 2.45
Dynamic thiolation-thioesterase structure of a non-ribosomal peptide synthetase. Nature (2008) 2.39
Identification, function and structure of the mycobacterial sulfotransferase that initiates sulfolipid-1 biosynthesis. Nat Struct Mol Biol (2004) 2.25
Nature's inventory of halogenation catalysts: oxidative strategies predominate. Chem Rev (2006) 2.24
Thirteen posttranslational modifications convert a 14-residue peptide into the antibiotic thiocillin. Proc Natl Acad Sci U S A (2009) 2.22
Facile detection of acyl and peptidyl intermediates on thiotemplate carrier domains via phosphopantetheinyl elimination reactions during tandem mass spectrometry. Biochemistry (2006) 2.22
The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis. Proc Natl Acad Sci U S A (2007) 2.22
SyrB2 in syringomycin E biosynthesis is a nonheme FeII alpha-ketoglutarate- and O2-dependent halogenase. Proc Natl Acad Sci U S A (2005) 2.12
Halogenation strategies in natural product biosynthesis. Chem Biol (2008) 2.08
Selective translocation of intracellular Smoothened to the primary cilium in response to Hedgehog pathway modulation. Proc Natl Acad Sci U S A (2009) 2.04
Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase. Proc Natl Acad Sci U S A (2005) 2.03
Vancomycin assembly: nature's way. Angew Chem Int Ed Engl (2003) 1.99
Lipidomics reveals control of Mycobacterium tuberculosis virulence lipids via metabolic coupling. Proc Natl Acad Sci U S A (2007) 1.98
Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases. ACS Chem Biol (2007) 1.94
Incorporation of glucose analogs by GtfE and GtfD from the vancomycin biosynthetic pathway to generate variant glycopeptides. Chem Biol (2002) 1.90
A singular enzymatic megacomplex from Bacillus subtilis. Proc Natl Acad Sci U S A (2006) 1.89
Labeling proteins with small molecules by site-specific posttranslational modification. J Am Chem Soc (2004) 1.84
The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains. Nat Struct Biol (2002) 1.82
Robust in vitro activity of RebF and RebH, a two-component reductase/halogenase, generating 7-chlorotryptophan during rebeccamycin biosynthesis. Proc Natl Acad Sci U S A (2005) 1.82
Site-specific protein labeling by Sfp phosphopantetheinyl transferase. Nat Protoc (2006) 1.82
Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis. Proc Natl Acad Sci U S A (2006) 1.80
Yersiniabactin synthetase: a four-protein assembly line producing the nonribosomal peptide/polyketide hybrid siderophore of Yersinia pestis. Chem Biol (2002) 1.75
A transglutaminase homologue as a condensation catalyst in antibiotic assembly lines. Nature (2007) 1.74
Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry. Mol Cell Proteomics (2007) 1.71
Chlorination by a long-lived intermediate in the mechanism of flavin-dependent halogenases. Biochemistry (2007) 1.69
Substrate-triggered formation and remarkable stability of the C-H bond-cleaving chloroferryl intermediate in the aliphatic halogenase, SyrB2. Biochemistry (2009) 1.69
Terrequinone A biosynthesis through L-tryptophan oxidation, dimerization and bisprenylation. Nat Chem Biol (2007) 1.68
Structural basis for the selectivity of the external thioesterase of the surfactin synthetase. Nature (2008) 1.66
Mass spectrometric interrogation of thioester-bound intermediates in the initial stages of epothilone biosynthesis. Chem Biol (2004) 1.61
The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Science (2010) 1.59
Compositional analysis and quantification of heparin and heparan sulfate by electrospray ionization ion trap mass spectrometry. Anal Chem (2003) 1.59
Dichlorination of a pyrrolyl-S-carrier protein by FADH2-dependent halogenase PltA during pyoluteorin biosynthesis. Proc Natl Acad Sci U S A (2005) 1.58
Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009. Proc Natl Acad Sci U S A (2002) 1.57
Total biosynthesis: in vitro reconstitution of polyketide and nonribosomal peptide pathways. Nat Prod Rep (2008) 1.56
An unusual role for a mobile flavin in StaC-like indolocarbazole biosynthetic enzymes. Chem Biol (2012) 1.56
Codon randomization for rapid exploration of chemical space in thiopeptide antibiotic variants. Chem Biol (2012) 1.55
Halogenation of unactivated carbon centers in natural product biosynthesis: trichlorination of leucine during barbamide biosynthesis. J Am Chem Soc (2006) 1.53
In vitro characterization of IroB, a pathogen-associated C-glycosyltransferase. Proc Natl Acad Sci U S A (2004) 1.52
Heparin sequencing using enzymatic digestion and ESI-MSn with HOST: a heparin/HS oligosaccharide sequencing tool. Anal Chem (2005) 1.52
Manipulation of thiocillin variants by prepeptide gene replacement: structure, conformation, and activity of heterocycle substitution mutants. J Am Chem Soc (2010) 1.51
Delineating mechanisms of dissociation for isomeric heparin disaccharides using isotope labeling and ion trap tandem mass spectrometry. J Am Soc Mass Spectrom (2004) 1.50
CloQ, a prenyltransferase involved in clorobiocin biosynthesis. Proc Natl Acad Sci U S A (2003) 1.50
Evidence for a monomeric structure of nonribosomal Peptide synthetases. Chem Biol (2002) 1.49
Structural basis for the cyclization of the lipopeptide antibiotic surfactin by the thioesterase domain SrfTE. Structure (2002) 1.49
Cyclopiazonic acid biosynthesis in Aspergillus sp.: characterization of a reductase-like R* domain in cyclopiazonate synthetase that forms and releases cyclo-acetoacetyl-L-tryptophan. Biochemistry (2009) 1.48
Substrate positioning controls the partition between halogenation and hydroxylation in the aliphatic halogenase, SyrB2. Proc Natl Acad Sci U S A (2009) 1.47
In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes. J Am Chem Soc (2005) 1.47
Crystal structure of vancosaminyltransferase GtfD from the vancomycin biosynthetic pathway: interactions with acceptor and nucleotide ligands. Biochemistry (2004) 1.45
Type II thioesterase restores activity of a NRPS module stalled with an aminoacyl-S-enzyme that cannot be elongated. Chembiochem (2004) 1.45
Structure of the TDP-epi-vancosaminyltransferase GtfA from the chloroeremomycin biosynthetic pathway. Proc Natl Acad Sci U S A (2003) 1.45
Non-uniformly sampled double-TROSY hNcaNH experiments for NMR sequential assignments of large proteins. J Am Chem Soc (2006) 1.45
Characterization of the cereulide NRPS alpha-hydroxy acid specifying modules: activation of alpha-keto acids and chiral reduction on the assembly line. J Am Chem Soc (2006) 1.44
Macrolactamization of glycosylated peptide thioesters by the thioesterase domain of tyrocidine synthetase. Chem Biol (2004) 1.44
Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis. Chem Biol (2002) 1.44
GliP, a multimodular nonribosomal peptide synthetase in Aspergillus fumigatus, makes the diketopiperazine scaffold of gliotoxin. Biochemistry (2006) 1.42
Identification of the biosynthetic gene cluster for the pacidamycin group of peptidyl nucleoside antibiotics. Proc Natl Acad Sci U S A (2010) 1.42
Substrate recognition, protein dynamics, and iron-sulfur cluster in Pseudomonas aeruginosa adenosine 5'-phosphosulfate reductase. J Mol Biol (2006) 1.42
Maturation of an Escherichia coli ribosomal peptide antibiotic by ATP-consuming N-P bond formation in microcin C7. J Am Chem Soc (2008) 1.41
Fungal indole alkaloid biosynthesis: genetic and biochemical investigation of the tryptoquialanine pathway in Penicillium aethiopicum. J Am Chem Soc (2011) 1.41
Investigating the initial steps in the biosynthesis of cyanobacterial sunscreen scytonemin. J Am Chem Soc (2008) 1.41
Formation of beta-hydroxy histidine in the biosynthesis of nikkomycin antibiotics. Chem Biol (2002) 1.41
CD and MCD of CytC3 and taurine dioxygenase: role of the facial triad in alpha-KG-dependent oxygenases. J Am Chem Soc (2007) 1.41
Spectroscopic evidence for a high-spin Br-Fe(IV)-oxo intermediate in the alpha-ketoglutarate-dependent halogenase CytC3 from Streptomyces. J Am Chem Soc (2007) 1.40
Structural insights into nonribosomal peptide enzymatic assembly lines. Nat Prod Rep (2009) 1.40
Biomimetic synthesis and optimization of cyclic peptide antibiotics. Nature (2002) 1.39
Staurosporine and rebeccamycin aglycones are assembled by the oxidative action of StaP, StaC, and RebC on chromopyrrolic acid. J Am Chem Soc (2006) 1.39
How the MccB bacterial ancestor of ubiquitin E1 initiates biosynthesis of the microcin C7 antibiotic. EMBO J (2009) 1.38
Compositional profiling of heparin/heparan sulfate using mass spectrometry: assay for specificity of a novel extracellular human endosulfatase. Glycobiology (2005) 1.35
A conserved mechanism for sulfonucleotide reduction. PLoS Biol (2005) 1.34
Enzymatic processing of fumiquinazoline F: a tandem oxidative-acylation strategy for the generation of multicyclic scaffolds in fungal indole alkaloid biosynthesis. Biochemistry (2010) 1.33
In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum. Biochemistry (2006) 1.32
Genetic interception and structural characterization of thiopeptide cyclization precursors from Bacillus cereus. J Am Chem Soc (2010) 1.31
Drugs as materials: valuing physical form in drug discovery. Nat Rev Drug Discov (2004) 1.31
Cloning and characterization of the biosynthetic gene cluster for kutznerides. Proc Natl Acad Sci U S A (2007) 1.31
Carrier protein structure and recognition in polyketide and nonribosomal peptide biosynthesis. Biochemistry (2006) 1.29
Generation of thiocillin variants by prepeptide gene replacement and in vivo processing by Bacillus cereus. J Am Chem Soc (2009) 1.28
A protein interaction surface in nonribosomal peptide synthesis mapped by combinatorial mutagenesis and selection. Proc Natl Acad Sci U S A (2006) 1.28
Flavin redox chemistry precedes substrate chlorination during the reaction of the flavin-dependent halogenase RebH. Biochemistry (2006) 1.28
Identification of novel protein kinase A phosphorylation sites in the M-domain of human and murine cardiac myosin binding protein-C using mass spectrometry analysis. J Proteome Res (2010) 1.28
Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach. Proc Natl Acad Sci U S A (2002) 1.26
PapA1 and PapA2 are acyltransferases essential for the biosynthesis of the Mycobacterium tuberculosis virulence factor sulfolipid-1. Proc Natl Acad Sci U S A (2007) 1.25
Enzymatic generation of the antimetabolite gamma,gamma-dichloroaminobutyrate by NRPS and mononuclear iron halogenase action in a streptomycete. Chem Biol (2006) 1.25
Anthranilate-activating modules from fungal nonribosomal peptide assembly lines. Biochemistry (2010) 1.25
Activity screening of carrier domains within nonribosomal peptide synthetases using complex substrate mixtures and large molecule mass spectrometry. Biochemistry (2006) 1.24
Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES-bound complexes. Protein Sci (2005) 1.23
GDE2 promotes neurogenesis by glycosylphosphatidylinositol-anchor cleavage of RECK. Science (2013) 1.23