Molecular cloning and characterization of Bifidobacterium bifidum 1,2-alpha-L-fucosidase (AfcA), a novel inverting glycosidase (glycoside hydrolase family 95).

PubWeight™: 1.72‹?› | Rank: Top 3%

🔗 View Article (PMC 451662)

Published in J Bacteriol on August 01, 2004

Authors

Takane Katayama1, Akiko Sakuma, Takatoshi Kimura, Yutaka Makimura, Jun Hiratake, Kanzo Sakata, Takashi Yamanoi, Hidehiko Kumagai, Kenji Yamamoto

Author Affiliations

1: Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. takane@kais.kyoto-u.ac.jp

Articles citing this

Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum. Microbiol Mol Biol Rev (2007) 4.13

Genome-scale analyses of health-promoting bacteria: probiogenomics. Nat Rev Microbiol (2008) 3.25

Human milk glycobiome and its impact on the infant gastrointestinal microbiota. Proc Natl Acad Sci U S A (2010) 2.34

Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging. Proc Natl Acad Sci U S A (2010) 2.14

Nursing our microbiota: molecular linkages between bifidobacteria and milk oligosaccharides. Trends Microbiol (2010) 2.10

Identification of N-acetylhexosamine 1-kinase in the complete lacto-N-biose I/galacto-N-biose metabolic pathway in Bifidobacterium longum. Appl Environ Microbiol (2007) 1.94

Bifidobacterium bifidum lacto-N-biosidase, a critical enzyme for the degradation of human milk oligosaccharides with a type 1 structure. Appl Environ Microbiol (2008) 1.52

Secretor genotype (FUT2 gene) is strongly associated with the composition of Bifidobacteria in the human intestine. PLoS One (2011) 1.49

Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol (2013) 1.45

Physiology of consumption of human milk oligosaccharides by infant gut-associated bifidobacteria. J Biol Chem (2011) 1.36

Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent. PLoS One (2013) 1.25

Mucin degradation by Bifidobacterium strains isolated from the human intestinal microbiota. Appl Environ Microbiol (2008) 1.24

Reprograming of gut microbiome energy metabolism by the FUT2 Crohn's disease risk polymorphism. ISME J (2014) 1.23

Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera. Nat Commun (2015) 1.22

Glycan complexity dictates microbial resource allocation in the large intestine. Nat Commun (2015) 1.21

Bifidobacterium longum subsp. infantis ATCC 15697 α-fucosidases are active on fucosylated human milk oligosaccharides. Appl Environ Microbiol (2011) 1.20

Faecal microbiota composition in adults is associated with the FUT2 gene determining the secretor status. PLoS One (2014) 1.13

Biochemical analysis of a beta-D-xylosidase and a bifunctional xylanase-ferulic acid esterase from a xylanolytic gene cluster in Prevotella ruminicola 23. J Bacteriol (2009) 1.08

Bifidobacteria isolated from infants and cultured on human milk oligosaccharides affect intestinal epithelial function. J Pediatr Gastroenterol Nutr (2012) 1.08

Identification of a GH110 subfamily of alpha 1,3-galactosidases: novel enzymes for removal of the alpha 3Gal xenotransplantation antigen. J Biol Chem (2008) 1.07

The impact of the milk glycobiome on the neonate gut microbiota. Annu Rev Anim Biosci (2014) 1.05

Mucin-type O-glycans and their roles in intestinal homeostasis. Glycobiology (2013) 0.98

Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217. Acta Crystallogr Sect F Struct Biol Cryst Commun (2007) 0.97

Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium. BMC Microbiol (2014) 0.95

Utilization of natural fucosylated oligosaccharides by three novel alpha-L-fucosidases from a probiotic Lactobacillus casei strain. Appl Environ Microbiol (2010) 0.94

The predominance of type I oligosaccharides is a feature specific to human breast milk. Adv Nutr (2012) 0.94

Bifidobacterial enzymes involved in the metabolism of human milk oligosaccharides. Adv Nutr (2012) 0.90

Enzymatic ability of Bifidobacterium animalis subsp. lactis to hydrolyze milk proteins: identification and characterization of endopeptidase O. Appl Environ Microbiol (2005) 0.89

Characterization of three beta-galactoside phosphorylases from Clostridium phytofermentans: discovery of d-galactosyl-beta1->4-l-rhamnose phosphorylase. J Biol Chem (2009) 0.88

Lacto-N-biosidase encoded by a novel gene of Bifidobacterium longum subspecies longum shows unique substrate specificity and requires a designated chaperone for its active expression. J Biol Chem (2013) 0.87

The crystal structure of galacto-N-biose/lacto-N-biose I phosphorylase: a large deformation of a TIM barrel scaffold. J Biol Chem (2009) 0.87

Genogroup IV and VI canine noroviruses interact with histo-blood group antigens. J Virol (2014) 0.87

α-N-acetylgalactosaminidase from infant-associated bifidobacteria belonging to novel glycoside hydrolase family 129 is implicated in alternative mucin degradation pathway. J Biol Chem (2011) 0.85

Chemical characterization of oligosaccharides in the milk of six species of New and Old World monkeys. Glycoconj J (2010) 0.83

Crystal structures of a glycoside hydrolase family 20 lacto-N-biosidase from Bifidobacterium bifidum. J Biol Chem (2013) 0.82

1,3-1,4-α-L-fucosynthase that specifically introduces Lewis a/x antigens into type-1/2 chains. J Biol Chem (2012) 0.80

Epithelial glycosylation in gut homeostasis and inflammation. Nat Immunol (2016) 0.79

Mucin-Microbiota Interaction During Postnatal Maturation of the Intestinal Ecosystem: Clinical Implications. Dig Dis Sci (2016) 0.78

Pyridylamination as a means of analyzing complex sugar chains. Proc Jpn Acad Ser B Phys Biol Sci (2010) 0.78

Characterization of an α-l-fucosidase from the periodontal pathogen Tannerella forsythia. Virulence (2015) 0.78

Structure of the fucose mutarotase from Streptococcus pneumoniae in complex with L-fucose. Acta Crystallogr Sect F Struct Biol Cryst Commun (2011) 0.77

Bifidobacterium longum subsp. longum Exo-β-1,3-Galactanase, an enzyme for the degradation of type II arabinogalactan. Appl Environ Microbiol (2014) 0.77

Proteinaceous Molecules Mediating Bifidobacterium-Host Interactions. Front Microbiol (2016) 0.75

The First Crystal Structure of a Family 129 Glycoside Hydrolase from a Probiotic Bacterium Reveals Critical Residues and Metal Co-factors. J Biol Chem (2017) 0.75

Bifidobacterium bifidum Extracellular Sialidase Enhances Adhesion to the Mucosal Surface and Supports Carbohydrate Assimilation. MBio (2017) 0.75

Articles cited by this

Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (1970) 1528.65

DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A (1977) 790.54

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res (1994) 392.47

Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng (1997) 38.38

Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A (1978) 19.34

A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J (1991) 14.56

PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biochem Sci (1999) 14.36

A genomic view of the human-Bacteroides thetaiotaomicron symbiosis. Science (2003) 12.57

Comparison of the genomes of two Xanthomonas pathogens with differing host specificities. Nature (2002) 8.42

Genome of the bacterium Streptococcus pneumoniae strain R6. J Bacteriol (2001) 7.97

Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res (1989) 7.78

Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope. Microbiol Mol Biol Rev (1999) 7.76

The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract. Proc Natl Acad Sci U S A (2002) 7.21

Helicobacter pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging. Science (1998) 6.99

Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater. Proc Natl Acad Sci U S A (2002) 5.40

Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis. Nucleic Acids Res (2000) 4.11

Campylobacter jejuni binds intestinal H(O) antigen (Fuc alpha 1, 2Gal beta 1, 4GlcNAc), and fucosyloligosaccharides of human milk inhibit its binding and infection. J Biol Chem (2003) 2.85

Molecular cloning of a cDNA for human alpha-L-fucosidase. Proc Natl Acad Sci U S A (1985) 2.14

Oligosaccharide structures of human colonic mucin. J Biol Chem (1985) 2.06

Structure analyses of oligosaccharides by tagging of the reducing end sugars with a fluorescent compound. Biochem Biophys Res Commun (1978) 1.25

Purification and characterization of novel glycosidases from the bacterial genus Xanthomonas. Glycobiology (1995) 1.17

P- and E-selectin use common sites for carbohydrate ligand recognition and cell adhesion. J Cell Biol (1993) 1.14

Enzymes that destroy blood group specificity. I. Purification and properties of alpha-L-fucosidase from Clostridium perfringens. J Biol Chem (1970) 1.10

Separation of glycoprotein-derived oligosaccharides by thin-layer chromatography. Anal Biochem (1979) 1.08

Identification of an endo-beta-N-acetylglucosaminidase gene in Caenorhabditis elegans and its expression in Escherichia coli. Glycobiology (2002) 1.05

Degradation of human intestinal glycosphingolipids by extracellular glycosidases from mucin-degrading bacteria of the human fecal flora. J Biol Chem (1988) 1.01

Cloning and random mutagenesis of the Erwinia herbicola tyrR gene for high-level expression of tyrosine phenol-lyase. Appl Environ Microbiol (2000) 0.97

A developmentally regulated gene product from Dictyostelium discoideum shows high homology to human alpha-L-fucosidase. FEBS Lett (1989) 0.95

Glycosidases of aspergillus niger. II. Purification and general properties of 1,2-alpha-L-fucosidase. J Biol Chem (1970) 0.94

alpha-L-fucosidases from almond emulsin: characterization of the two enzymes with different specificities. Arch Biochem Biophys (1977) 0.93

Transcriptional regulation of tyrosine phenol-lyase gene of Erwinia herbicola AJ2985. Biosci Biotechnol Biochem (1995) 0.92

Isolation and sequence analysis of a cDNA encoding rat liver alpha-L-fucosidase. Biochem J (1989) 0.92

Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Sulfolobus solfataricus via chemical rescue of an inactive mutant. Biochemistry (2003) 0.90

Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis. J Biol Chem (2003) 0.87

Cloning and nucleotide sequence of the beta-D-glucosidase gene from Bifidobacterium breve clb, and expression of beta-D-glucosidase activity in Escherichia coli. Biosci Biotechnol Biochem (1996) 0.87

Purification and characterization of alpha-L-fucosidase from Streptomyces species. J Biol Chem (1992) 0.87

Colorimetric assay for free and bound L-fucose. Anal Biochem (1989) 0.83

Analysis of neutral saccharides in human milk derivatized with 2-aminoacridone by capillary electrophoresis with laser-induced fluorescence detection. Anal Biochem (2002) 0.83

Abnormally fucosylated haptoglobin as a marker for alcoholic liver disease but not excessive alcohol consumption or non-alcoholic liver disease. Clin Chim Acta (1993) 0.82

An alpha-L-fucosidase from Thermus sp. with unusually broad specificity. Glycoconj J (2001) 0.81

Efficient production and purification of extracellular 1,2-alpha-L-fucosidase of Bacillus sp. K40T. Biosci Biotechnol Biochem (1995) 0.79

Purification and characterization of alpha-L-fucosidase from Bacillus circulans grown on porcine gastric mucin. J Biochem (1990) 0.79

Isolation of the canine alpha-L-fucosidase cDNA and definition of the fucosidosis mutation in English Springer Spaniels. Mamm Genome (1996) 0.78

Articles by these authors

Genome and virulence determinants of high virulence community-acquired MRSA. Lancet (2002) 14.64

Structural basis for the spectral difference in luciferase bioluminescence. Nature (2006) 3.60

Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat Biotechnol (2005) 2.89

Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid. Plant Physiol (2004) 2.08

Effect of a Japanese energy healing method known as Johrei on viability and proliferation of cultured cancer cells in vitro. J Altern Complement Med (2012) 1.96

On the cyto-toxicity caused by quantum dots. Microbiol Immunol (2004) 1.90

Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker. Biochem Biophys Res Commun (2003) 1.90

Microbial production of plant benzylisoquinoline alkaloids. Proc Natl Acad Sci U S A (2008) 1.89

Inhibition of dendritic spine morphogenesis and synaptic transmission by activity-inducible protein Homer1a. J Neurosci (2003) 1.77

Mutants of Mucor hiemalis endo-beta-N-acetylglucosaminidase show enhanced transglycosylation and glycosynthase-like activities. J Biol Chem (2007) 1.62

Purification, gene cloning, gene expression, and mutants of Dps from the obligate anaerobe Porphyromonas gingivalis. Infect Immun (2003) 1.57

Bifidobacterium bifidum lacto-N-biosidase, a critical enzyme for the degradation of human milk oligosaccharides with a type 1 structure. Appl Environ Microbiol (2008) 1.52

Identification and molecular cloning of a novel glycoside hydrolase family of core 1 type O-glycan-specific endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum. J Biol Chem (2005) 1.51

A bacterial platform for fermentative production of plant alkaloids. Nat Commun (2011) 1.48

Water-soluble photoluminescent silicon quantum dots. Angew Chem Int Ed Engl (2005) 1.45

C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis. Plant Cell (2006) 1.44

MPO-ANCA induces IL-17 production by activated neutrophils in vitro via classical complement pathway-dependent manner. J Autoimmun (2008) 1.42

A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12. J Biol Chem (2004) 1.42

Applications of T-lymphoma labeled with fluorescent quantum dots to cell tracing markers in mouse body. Biochem Biophys Res Commun (2004) 1.41

Gpr1, a putative G-protein-coupled receptor, regulates morphogenesis and hypha formation in the pathogenic fungus Candida albicans. Eukaryot Cell (2004) 1.40

Two distinct alpha-L-fucosidases from Bifidobacterium bifidum are essential for the utilization of fucosylated milk oligosaccharides and glycoconjugates. Glycobiology (2009) 1.37

Physiology of consumption of human milk oligosaccharides by infant gut-associated bifidobacteria. J Biol Chem (2011) 1.36

Injection of PEGylated liposomes in rats elicits PEG-specific IgM, which is responsible for rapid elimination of a second dose of PEGylated liposomes. J Control Release (2006) 1.36

Construction and characterization of a nonpigmented mutant of Porphyromonas gingivalis: cell surface polysaccharide as an anchorage for gingipains. Microbiology (2002) 1.34

Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae: a novel probe for core fucose. J Biol Chem (2007) 1.33

Characterization of heterotrimeric G protein complexes in rice plasma membrane. Plant J (2004) 1.29

Chemoenzymatic synthesis and application of glycopolymers containing multivalent sialyloligosaccharides with a poly(L-glutamic acid) backbone for inhibition of infection by influenza viruses. Glycobiology (2002) 1.24

Acquisition and contextual switching of multiple internal models for different viscous force fields. Neurosci Res (2003) 1.24

Crystal structures of gamma-glutamyltranspeptidase from Escherichia coli, a key enzyme in glutathione metabolism, and its reaction intermediate. Proc Natl Acad Sci U S A (2006) 1.22

Scopoletin is biosynthesized via ortho-hydroxylation of feruloyl CoA by a 2-oxoglutarate-dependent dioxygenase in Arabidopsis thaliana. Plant J (2008) 1.22

Glycosynthases enable a highly efficient chemoenzymatic synthesis of N-glycoproteins carrying intact natural N-glycans. J Am Chem Soc (2009) 1.21

LPT1 encodes a membrane-bound O-acyltransferase involved in the acylation of lysophospholipids in the yeast Saccharomyces cerevisiae. J Biol Chem (2007) 1.21

Autocatalytic processing of gamma-glutamyltranspeptidase. J Biol Chem (2002) 1.21

gamma-Glutamylputrescine synthetase in the putrescine utilization pathway of Escherichia coli K-12. J Biol Chem (2008) 1.20

Accumulation of coumarins in Arabidopsis thaliana. Phytochemistry (2006) 1.19

Structural basis of the catalytic reaction mechanism of novel 1,2-alpha-L-fucosidase from Bifidobacterium bifidum. J Biol Chem (2007) 1.19

Chemical structure and immunobiological activity of Porphyromonas gingivalis lipid A. Front Biosci (2007) 1.18

The yliA, -B, -C, and -D genes of Escherichia coli K-12 encode a novel glutathione importer with an ATP-binding cassette. J Bacteriol (2005) 1.18

Structural and thermodynamic analyses of solute-binding Protein from Bifidobacterium longum specific for core 1 disaccharide and lacto-N-biose I. J Biol Chem (2008) 1.17

Arthrobacter endo-beta-N-acetylglucosaminidase shows transglycosylation activity on complex-type N-glycan oxazolines: one-pot conversion of ribonuclease B to sialylated ribonuclease C. Chembiochem (2010) 1.17

Effective production of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR. J Biotechnol (2005) 1.15

Structural basis for gating mechanisms of a eukaryotic P-glycoprotein homolog. Proc Natl Acad Sci U S A (2014) 1.14

Quantum dots targeted to the assigned organelle in living cells. Microbiol Immunol (2004) 1.13

Autoprocessing of Helicobacter pylori gamma-glutamyltranspeptidase leads to the formation of a threonine-threonine catalytic dyad. J Biol Chem (2006) 1.12

Ranavirus outbreak in North American bullfrogs (Rana catesbeiana), Japan, 2008. Emerg Infect Dis (2009) 1.12

Crystal structure of gamma-glutamylcysteine synthetase: insights into the mechanism of catalysis by a key enzyme for glutathione homeostasis. Proc Natl Acad Sci U S A (2004) 1.11

Inhibition of CCL1-CCR8 interaction prevents aggregation of macrophages and development of peritoneal adhesions. J Immunol (2007) 1.11

Arabidopsis CYP90B1 catalyses the early C-22 hydroxylation of C27, C28 and C29 sterols. Plant J (2006) 1.10

Chemical reactions on surface molecules attached to silicon quantum dots. J Am Chem Soc (2010) 1.10

Conjugation of quantum dots and JT95 IgM monoclonal antibody for thyroid carcinoma without abolishing the specificity and activity of the antibody. IEEE Trans Nanobioscience (2011) 1.10

Cooperation of β-galactosidase and β-N-acetylhexosaminidase from bifidobacteria in assimilation of human milk oligosaccharides with type 2 structure. Glycobiology (2010) 1.10

Role of a PA14 domain in determining substrate specificity of a glycoside hydrolase family 3 β-glucosidase from Kluyveromyces marxianus. Biochem J (2010) 1.09

Association of the neuronal cell adhesion molecule (NRCAM) gene variants with autism. Int J Neuropsychopharmacol (2008) 1.09

Use of fluorescent quantum dot bioconjugates for cellular imaging of immune cells, cell organelle labeling, and nanomedicine: surface modification regulates biological function, including cytotoxicity. J Artif Organs (2007) 1.08

Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato. Plant Cell (2006) 1.07

A novel type of two-component regulatory system affecting gingipains in Porphyromonas gingivalis. Microbiol Immunol (2003) 1.07

Cathepsin E prevents tumor growth and metastasis by catalyzing the proteolytic release of soluble TRAIL from tumor cell surface. Cancer Res (2007) 1.07

Analysis of the conformational energy landscape of human snRNA with a metric based on tree representation of RNA structures. Nucleic Acids Res (2003) 1.06

Construction of thermotolerant yeast expressing thermostable cellulase genes. J Biotechnol (2007) 1.06

Chemical profiling and gene expression profiling during the manufacturing process of Taiwan oolong tea "Oriental Beauty". Biosci Biotechnol Biochem (2007) 1.06

Identification of an endo-beta-N-acetylglucosaminidase gene in Caenorhabditis elegans and its expression in Escherichia coli. Glycobiology (2002) 1.05

Bifidobacterium longum subsp. infantis uses two different β-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharides. Glycobiology (2011) 1.05

Gamma-glutamyl-gamma-aminobutyrate hydrolase in the putrescine utilization pathway of Escherichia coli K-12. FEMS Microbiol Lett (2006) 1.04

Efficient glycosynthase mutant derived from Mucor hiemalis endo-beta-N-acetylglucosaminidase capable of transferring oligosaccharide from both sugar oxazoline and natural N-glycan. J Biol Chem (2009) 1.04

Suppression of pathogenicity of Porphyromonas gingivalis by newly developed gingipain inhibitors. Mol Pharmacol (2004) 1.04

Crystal structure of the gamma-glutamyltranspeptidase precursor protein from Escherichia coli. Structural changes upon autocatalytic processing and implications for the maturation mechanism. J Biol Chem (2006) 1.04

Fragile X carrier screening and FMR1 allele distribution in the Japanese population. Brain Dev (2009) 1.02

An exo-alpha-sialidase from bifidobacteria involved in the degradation of sialyloligosaccharides in human milk and intestinal glycoconjugates. Glycobiology (2010) 1.02

Toxicity of nanocrystal quantum dots: the relevance of surface modifications. Arch Toxicol (2011) 1.02

Novel bifidobacterial glycosidases acting on sugar chains of mucin glycoproteins. J Biosci Bioeng (2005) 1.01

A putrescine-inducible pathway comprising PuuE-YneI in which gamma-aminobutyrate is degraded into succinate in Escherichia coli K-12. J Bacteriol (2010) 1.01

Characterization of two different endo-alpha-N-acetylgalactosaminidases from probiotic and pathogenic enterobacteria, Bifidobacterium longum and Clostridium perfringens. Glycobiology (2008) 1.01

Identification of the LIV-I/LS system as the third phenylalanine transporter in Escherichia coli K-12. J Bacteriol (2004) 1.00

A role for gingipains in cellular responses and bacterial survival in Porphyromonas gingivalis-infected cells. Front Biosci (2007) 0.99

Cloning and functional expression of thermostable beta-glucosidase gene from Thermoascus aurantiacus. Appl Microbiol Biotechnol (2006) 0.99

Design, synthesis, and evaluation of gamma-phosphono diester analogues of glutamate as highly potent inhibitors and active site probes of gamma-glutamyl transpeptidase. Biochemistry (2007) 0.99

The putrescine Importer PuuP of Escherichia coli K-12. J Bacteriol (2009) 0.99

Enzymatic synthesis of gamma-glutamylvaline to improve the bitter taste of valine. J Agric Food Chem (2004) 0.98

Porphyromonas gingivalis-induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase. Mol Microbiol (2006) 0.97

Improvement of the bitter taste of amino acids through the transpeptidation reaction of bacterial gamma-glutamyltranspeptidase. J Agric Food Chem (2002) 0.97

Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217. Acta Crystallogr Sect F Struct Biol Cryst Commun (2007) 0.97

Efficient transfer of sialo-oligosaccharide onto proteins by combined use of a glycosynthase-like mutant of Mucor hiemalis endoglycosidase and synthetic sialo-complex-type sugar oxazoline. Biochim Biophys Acta (2010) 0.96

Luminescent passive-oxidized silicon quantum dots as biological staining labels and their cytotoxicity effects at high concentration. Nanotechnology (2008) 0.96

A functional virulence complex composed of gingipains, adhesins, and lipopolysaccharide shows high affinity to host cells and matrix proteins and escapes recognition by host immune systems. Infect Immun (2005) 0.96

Glucosamine induces autophagy via an mTOR-independent pathway. Biochem Biophys Res Commun (2010) 0.96

Cathepsin E deficiency induces a novel form of lysosomal storage disorder showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH in macrophages. J Biol Chem (2006) 0.96

Combination of real-value smell and metaphor expression aids yeast detection. PLoS One (2009) 0.95

A plant growth retardant, uniconazole, is a potent inhibitor of ABA catabolism in Arabidopsis. Biosci Biotechnol Biochem (2006) 0.95