Microbial production of plant benzylisoquinoline alkaloids.

Complete biosynthesis of opioids in yeast. Science (2015) 2.78

Production of benzylisoquinoline alkaloids in Saccharomyces cerevisiae. Nat Chem Biol (2008) 2.19

A microbial biomanufacturing platform for natural and semisynthetic opioids. Nat Chem Biol (2014) 1.57

An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose. Nat Chem Biol (2015) 1.56

A bacterial platform for fermentative production of plant alkaloids. Nat Commun (2011) 1.48

Opportunities in metabolic engineering to facilitate scalable alkaloid production. Nat Chem Biol (2009) 1.42

Dioxygenases catalyze the O-demethylation steps of morphine biosynthesis in opium poppy. Nat Chem Biol (2010) 1.39

Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol (2011) 1.31

CYP719B1 is salutaridine synthase, the C-C phenol-coupling enzyme of morphine biosynthesis in opium poppy. J Biol Chem (2009) 1.24

Metabolic engineering for the production of natural products. Annu Rev Chem Biomol Eng (2011) 1.22

Engineering microbial factories for synthesis of value-added products. J Ind Microbiol Biotechnol (2011) 1.13

Engineered biosynthesis of bacterial aromatic polyketides in Escherichia coli. Proc Natl Acad Sci U S A (2008) 1.12

De novo production of the plant-derived alkaloid strictosidine in yeast. Proc Natl Acad Sci U S A (2015) 1.10

Distributing a metabolic pathway among a microbial consortium enhances production of natural products. Nat Biotechnol (2015) 1.06

Integrating carbon-halogen bond formation into medicinal plant metabolism. Nature (2010) 1.06

Metabolic reprogramming of periwinkle plant culture. Nat Chem Biol (2009) 1.04

Norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family. Plant Cell (2010) 1.04

Distinct mechanisms for spiro-carbon formation reveal biosynthetic pathway crosstalk. Nat Chem Biol (2013) 1.02

Biocatalytic organic synthesis of optically pure (S)-scoulerine and berbine and benzylisoquinoline alkaloids. J Org Chem (2011) 0.97

A retrosynthetic biology approach to metabolic pathway design for therapeutic production. BMC Syst Biol (2011) 0.95

Engineered biosynthesis of natural products in heterologous hosts. Chem Soc Rev (2015) 0.95

Removal of substrate inhibition and increase in maximal velocity in the short chain dehydrogenase/reductase salutaridine reductase involved in morphine biosynthesis. J Biol Chem (2009) 0.94

Benzylisoquinoline alkaloid biosynthesis in opium poppy. Planta (2014) 0.92

De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast. Metab Eng (2015) 0.91

Engineering strategies for the fermentative production of plant alkaloids in yeast. Metab Eng (2015) 0.91

Recent progress in the metabolic engineering of alkaloids in plant systems. Curr Opin Biotechnol (2012) 0.90

(R,S)-tetrahydropapaveroline production by stepwise fermentation using engineered Escherichia coli. Sci Rep (2014) 0.90

'Dopamine-first' mechanism enables the rational engineering of the norcoclaurine synthase aldehyde activity profile. FEBS J (2015) 0.88

Metabolic engineering of strains: from industrial-scale to lab-scale chemical production. J Ind Microbiol Biotechnol (2014) 0.85

Finding metabolic pathways using atom tracking. Bioinformatics (2010) 0.85

Optimization of yeast-based production of medicinal protoberberine alkaloids. Microb Cell Fact (2015) 0.82

Genes encoding norcoclaurine synthase occur as tandem fusions in the Papaveraceae. Sci Rep (2016) 0.82

Engineering microbial cells for the biosynthesis of natural compounds of pharmaceutical significance. Biomed Res Int (2013) 0.80

Comparative analysis of transcription factor gene families from Papaver somniferum: identification of regulatory factors involved in benzylisoquinoline alkaloid biosynthesis. Protoplasma (2015) 0.80

Development of bio-based fine chemical production through synthetic bioengineering. Microb Cell Fact (2014) 0.80

Total biosynthesis of opiates by stepwise fermentation using engineered Escherichia coli. Nat Commun (2016) 0.79

BIAdb: a curated database of benzylisoquinoline alkaloids. BMC Pharmacol (2010) 0.79

Enzyme catalysed Pictet-Spengler formation of chiral 1,1'-disubstituted- and spiro-tetrahydroisoquinolines. Nat Commun (2017) 0.79

The role of biocatalysis in the asymmetric synthesis of alkaloids. RSC Adv (2013) 0.77

Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines. Metab Eng (2016) 0.76

Molecular characterization of O-methyltransferases involved in isoquinoline alkaloid biosynthesis in Coptis japonica. Proc Jpn Acad Ser B Phys Biol Sci (2010) 0.76

Production platforms for the molecular pharming of alkaloid diversity. Proc Natl Acad Sci U S A (2008) 0.76

Microbial production of isoquinoline alkaloids as plant secondary metabolites based on metabolic engineering research. Proc Jpn Acad Ser B Phys Biol Sci (2013) 0.75

Joining Forces: Fermentation and Organic Synthesis for the Production of Complex Heterocycles. J Org Chem (2016) 0.75

Recent advances in the elucidation of enzymatic function in natural product biosynthesis. F1000Res (2015) 0.75

Characterization of the Promoter Region of Biosynthetic Enzyme Genes Involved in Berberine Biosynthesis in Coptis japonica. Front Plant Sci (2016) 0.75

Evaluation of Brachypodium distachyon L-Tyrosine Decarboxylase Using L-Tyrosine Over-Producing Saccharomyces cerevisiae. PLoS One (2015) 0.75

Structural evidence for the dopamine-first mechanism of norcoclaurine synthase. Biochemistry (2017) 0.75

Yeast factories for the production of aromatic compounds: from building blocks to plant secondary metabolites. J Ind Microbiol Biotechnol (2016) 0.75

Transformation of intact yeast cells treated with alkali cations. J Bacteriol (1983) 100.26

Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature (2006) 13.86

Berberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states. Diabetes (2006) 4.14

Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins. Nat Med (2004) 3.92

RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy. Nat Biotechnol (2004) 2.00

Localization of enzymes and alkaloidal metabolites in Papaver latex. Arch Biochem Biophys (1983) 1.67

Molecular cloning and characterization of norcoclaurine synthase, an enzyme catalyzing the first committed step in benzylisoquinoline alkaloid biosynthesis. Plant J (2004) 1.37

Expression of rat liver cytochrome P-450MC cDNA in Saccharomyces cerevisiae. DNA (1985) 1.35

Molecular characterization of the S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase involved in isoquinoline alkaloid biosynthesis in Coptis japonica. J Biol Chem (2000) 1.22

Molecular cloning and characterization of CYP80G2, a cytochrome P450 that catalyzes an intramolecular C-C phenol coupling of (S)-reticuline in magnoflorine biosynthesis, from cultured Coptis japonica cells. J Biol Chem (2008) 1.21

Molecular cloning and characterization of CYP719, a methylenedioxy bridge-forming enzyme that belongs to a novel P450 family, from cultured Coptis japonica cells. J Biol Chem (2003) 1.21

Mechanistic studies on norcoclaurine synthase of benzylisoquinoline alkaloid biosynthesis: an enzymatic Pictet-Spengler reaction. Biochemistry (2007) 1.15

Functional analysis of norcoclaurine synthase in Coptis japonica. J Biol Chem (2007) 1.13

Molecular cloning and characterization of coclaurine N-methyltransferase from cultured cells of Coptis japonica. J Biol Chem (2001) 1.10

Microbial transformation of alkaloids. Curr Opin Microbiol (2002) 1.05

Subcellular Localization of Alkaloids and Dopamine in Different Vacuolar Compartments of Papaver bracteatum. Plant Physiol (1986) 1.01

Purification and characterization of norcoclaurine synthase. The first committed enzyme in benzylisoquinoline alkaloid biosynthesis in plants. J Biol Chem (2002) 0.99

Poppy alkaloid profiling by electrospray tandem mass spectrometry and electrospray FT-ICR mass spectrometry after [ring-13C6]-tyramine feeding. Phytochemistry (2006) 0.99

Metabolic engineering in isoquinoline alkaloid biosynthesis. Curr Pharm Biotechnol (2007) 0.97

Magnoflorine from Coptidis Rhizoma protects high density lipoprotein during oxidant stress. Biol Pharm Bull (2007) 0.97

Knockdown of berberine bridge enzyme by RNAi accumulates (S)-reticuline and activates a silent pathway in cultured California poppy cells. Transgenic Res (2006) 0.92

Protective effect of magnoflorine isolated from coptidis rhizoma on Cu2+-induced oxidation of human low density lipoprotein. Planta Med (2007) 0.91

Overexpression of Coptis japonica norcoclaurine 6-O-methyltransferase overcomes the rate-limiting step in Benzylisoquinoline alkaloid biosynthesis in cultured Eschscholzia californica. Plant Cell Physiol (2006) 0.90

High-yield expression and purification of isotopically labeled norcoclaurine synthase, a Bet v 1-homologous enzyme, from Thalictrum flavum for NMR studies. Protein Expr Purif (2007) 0.87

Organella-targeted expression of rat liver cytochrome P450c27 in yeast. Genetically engineered alteration of mitochondrial P450 into a microsomal form creates a novel functional electron transport chain. J Biol Chem (1992) 0.86

Purification, cloning, and three-dimensional structure prediction of Micrococcus luteus FAD-containing tyramine oxidase. Biochem Biophys Res Commun (2000) 0.83

In vivo bioconversion of tetrahydroisoquinoline by recombinant coclaurine N-methyltransferase. Biosci Biotechnol Biochem (2004) 0.82

Potential cancer chemopreventive activity of simple isoquinolines, 1-benzylisoquinolines, and protoberberines. Phytochemistry (2005) 0.80

Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana. Genome Res (2007) 3.13

PGP4, an ATP binding cassette P-glycoprotein, catalyzes auxin transport in Arabidopsis thaliana roots. Plant Cell (2005) 2.08

Effects of diet and exercise on muscle and liver intracellular lipid contents and insulin sensitivity in type 2 diabetic patients. J Clin Endocrinol Metab (2005) 1.90

Molecular cloning and characterization of Bifidobacterium bifidum 1,2-alpha-L-fucosidase (AfcA), a novel inverting glycosidase (glycoside hydrolase family 95). J Bacteriol (2004) 1.72

Bifidobacterium bifidum lacto-N-biosidase, a critical enzyme for the degradation of human milk oligosaccharides with a type 1 structure. Appl Environ Microbiol (2008) 1.52

Identification and molecular cloning of a novel glycoside hydrolase family of core 1 type O-glycan-specific endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum. J Biol Chem (2005) 1.51

A bacterial platform for fermentative production of plant alkaloids. Nat Commun (2011) 1.48

Involvement of CjMDR1, a plant multidrug-resistance-type ATP-binding cassette protein, in alkaloid transport in Coptis japonica. Proc Natl Acad Sci U S A (2003) 1.45

Nitrogen recycling and remobilization are differentially controlled by leaf senescence and development stage in Arabidopsis under low nitrogen nutrition. Plant Physiol (2008) 1.42

A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12. J Biol Chem (2004) 1.42

Gpr1, a putative G-protein-coupled receptor, regulates morphogenesis and hypha formation in the pathogenic fungus Candida albicans. Eukaryot Cell (2004) 1.40

Two distinct alpha-L-fucosidases from Bifidobacterium bifidum are essential for the utilization of fucosylated milk oligosaccharides and glycoconjugates. Glycobiology (2009) 1.37

Physiology of consumption of human milk oligosaccharides by infant gut-associated bifidobacteria. J Biol Chem (2011) 1.36

Repetitive fluctuations in blood glucose enhance monocyte adhesion to the endothelium of rat thoracic aorta. Arterioscler Thromb Vasc Biol (2006) 1.35

Characterization of heterotrimeric G protein complexes in rice plasma membrane. Plant J (2004) 1.29

Geranyl diphosphate:4-hydroxybenzoate geranyltransferase from Lithospermum erythrorhizon. Cloning and characterization of a ket enzyme in shikonin biosynthesis. J Biol Chem (2001) 1.26

PsbP protein, but not PsbQ protein, is essential for the regulation and stabilization of photosystem II in higher plants. Plant Physiol (2005) 1.23

Crystal structures of gamma-glutamyltranspeptidase from Escherichia coli, a key enzyme in glutathione metabolism, and its reaction intermediate. Proc Natl Acad Sci U S A (2006) 1.22

Molecular cloning and characterization of CYP80G2, a cytochrome P450 that catalyzes an intramolecular C-C phenol coupling of (S)-reticuline in magnoflorine biosynthesis, from cultured Coptis japonica cells. J Biol Chem (2008) 1.21

LPT1 encodes a membrane-bound O-acyltransferase involved in the acylation of lysophospholipids in the yeast Saccharomyces cerevisiae. J Biol Chem (2007) 1.21

Distinct functions for the two PsbP-like proteins PPL1 and PPL2 in the chloroplast thylakoid lumen of Arabidopsis. Plant Physiol (2007) 1.21

Autocatalytic processing of gamma-glutamyltranspeptidase. J Biol Chem (2002) 1.21

Molecular cloning and characterization of CYP719, a methylenedioxy bridge-forming enzyme that belongs to a novel P450 family, from cultured Coptis japonica cells. J Biol Chem (2003) 1.21

gamma-Glutamylputrescine synthetase in the putrescine utilization pathway of Escherichia coli K-12. J Biol Chem (2008) 1.20

Structural basis of the catalytic reaction mechanism of novel 1,2-alpha-L-fucosidase from Bifidobacterium bifidum. J Biol Chem (2007) 1.19

The yliA, -B, -C, and -D genes of Escherichia coli K-12 encode a novel glutathione importer with an ATP-binding cassette. J Bacteriol (2005) 1.18

Structural and thermodynamic analyses of solute-binding Protein from Bifidobacterium longum specific for core 1 disaccharide and lacto-N-biose I. J Biol Chem (2008) 1.17

Biochemical conservation and evolution of germacrene A oxidase in asteraceae. J Biol Chem (2010) 1.15

Effective production of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR. J Biotechnol (2005) 1.15

Characterization of an Arabidopsis thaliana mutant with impaired psbO, one of two genes encoding extrinsic 33-kDa proteins in photosystem II. FEBS Lett (2002) 1.14

Characterization of vacuolar transport of the endogenous alkaloid berberine in Coptis japonica. Plant Physiol (2005) 1.13

Functional analysis of norcoclaurine synthase in Coptis japonica. J Biol Chem (2007) 1.13

Crystal structure of the PsbP protein of photosystem II from Nicotiana tabacum. EMBO Rep (2004) 1.12

Autoprocessing of Helicobacter pylori gamma-glutamyltranspeptidase leads to the formation of a threonine-threonine catalytic dyad. J Biol Chem (2006) 1.12

Molecular cloning and characterization of coclaurine N-methyltransferase from cultured cells of Coptis japonica. J Biol Chem (2001) 1.10

Defucosylated anti-CCR4 monoclonal antibody exercises potent ADCC-mediated antitumor effect in the novel tumor-bearing humanized NOD/Shi-scid, IL-2Rgamma(null) mouse model. Cancer Immunol Immunother (2008) 1.10

Identification of a WRKY protein as a transcriptional regulator of benzylisoquinoline alkaloid biosynthesis in Coptis japonica. Plant Cell Physiol (2006) 1.10

Cooperation of β-galactosidase and β-N-acetylhexosaminidase from bifidobacteria in assimilation of human milk oligosaccharides with type 2 structure. Glycobiology (2010) 1.10

Role of a PA14 domain in determining substrate specificity of a glycoside hydrolase family 3 β-glucosidase from Kluyveromyces marxianus. Biochem J (2010) 1.09

Nateglinide reduces carotid intima-media thickening in type 2 diabetic patients under good glycemic control. Arterioscler Thromb Vasc Biol (2007) 1.09

Unusual P450 reactions in plant secondary metabolism. Arch Biochem Biophys (2010) 1.08

Functional analysis of Arabidopsis ethylene-responsive element binding protein conferring resistance to Bax and abiotic stress-induced plant cell death. Plant Physiol (2005) 1.08

The DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco. Planta (2004) 1.07

Differential use of two cyclic electron flows around photosystem I for driving CO2-concentration mechanism in C4 photosynthesis. Proc Natl Acad Sci U S A (2005) 1.07

Construction of thermotolerant yeast expressing thermostable cellulase genes. J Biotechnol (2007) 1.06

Identification of an endo-beta-N-acetylglucosaminidase gene in Caenorhabditis elegans and its expression in Escherichia coli. Glycobiology (2002) 1.05

Bifidobacterium longum subsp. infantis uses two different β-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharides. Glycobiology (2011) 1.05

Gamma-glutamyl-gamma-aminobutyrate hydrolase in the putrescine utilization pathway of Escherichia coli K-12. FEMS Microbiol Lett (2006) 1.04

Crystal structure of the gamma-glutamyltranspeptidase precursor protein from Escherichia coli. Structural changes upon autocatalytic processing and implications for the maturation mechanism. J Biol Chem (2006) 1.04

Functional dissection of two Arabidopsis PsbO proteins: PsbO1 and PsbO2. FEBS J (2005) 1.03

Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica. FEBS J (2007) 1.03

An exo-alpha-sialidase from bifidobacteria involved in the degradation of sialyloligosaccharides in human milk and intestinal glycoconjugates. Glycobiology (2010) 1.02

A putrescine-inducible pathway comprising PuuE-YneI in which gamma-aminobutyrate is degraded into succinate in Escherichia coli K-12. J Bacteriol (2010) 1.01

Identification of the LIV-I/LS system as the third phenylalanine transporter in Escherichia coli K-12. J Bacteriol (2004) 1.00

Listeria monocytogenes strain-specific impairment of the TetR regulator underlies the drastic increase in cyclic di-AMP secretion and beta interferon-inducing ability. Infect Immun (2012) 0.99

The putrescine Importer PuuP of Escherichia coli K-12. J Bacteriol (2009) 0.99

Cloning and functional expression of thermostable beta-glucosidase gene from Thermoascus aurantiacus. Appl Microbiol Biotechnol (2006) 0.99

Enzymatic synthesis of gamma-glutamylvaline to improve the bitter taste of valine. J Agric Food Chem (2004) 0.98

The PsbQ protein stabilizes the functional binding of the PsbP protein to photosystem II in higher plants. Biochim Biophys Acta (2012) 0.98

Three novel subunits of Arabidopsis chloroplastic NAD(P)H dehydrogenase identified by bioinformatic and reverse genetic approaches. Plant J (2008) 0.98

Improvement of the bitter taste of amino acids through the transpeptidation reaction of bacterial gamma-glutamyltranspeptidase. J Agric Food Chem (2002) 0.97

Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217. Acta Crystallogr Sect F Struct Biol Cryst Commun (2007) 0.97

Metabolic engineering in isoquinoline alkaloid biosynthesis. Curr Pharm Biotechnol (2007) 0.97

Ribosomal RNA processing and an RNase R family member in chloroplasts of Arabidopsis. Plant Mol Biol (2004) 0.96

Molecular functions of PsbP and PsbQ proteins in the photosystem II supercomplex. J Photochem Photobiol B (2011) 0.96

NDF6: a thylakoid protein specific to terrestrial plants is essential for activity of chloroplastic NAD(P)H dehydrogenase in Arabidopsis. Plant Cell Physiol (2008) 0.96

Transcriptional activation of phosphoenolpyruvate carboxylase by phosphorus deficiency in tobacco. J Exp Bot (2003) 0.96

A role of TCP1 in the longitudinal elongation of leaves in Arabidopsis. Biosci Biotechnol Biochem (2010) 0.96

A regulatory cascade involving class II ETHYLENE RESPONSE FACTOR transcriptional repressors operates in the progression of leaf senescence. Plant Physiol (2013) 0.95

Three PsbQ-like proteins are required for the function of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis. Plant Cell Physiol (2010) 0.94

Bench-top fermentative production of plant benzylisoquinoline alkaloids using a bacterial platform. Bioeng Bugs (2012) 0.94

Defucosylated anti-CCR4 monoclonal antibody exerts potent ADCC against primary ATLL cells mediated by autologous human immune cells in NOD/Shi-scid, IL-2R gamma(null) mice in vivo. J Immunol (2009) 0.94

Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction. J Exp Bot (2003) 0.93

CYP719A subfamily of cytochrome P450 oxygenases and isoquinoline alkaloid biosynthesis in Eschscholzia californica. Plant Cell Rep (2008) 0.93

Characterization of berberine transport into Coptis japonica cells and the involvement of ABC protein. J Exp Bot (2002) 0.93

Geranylgeranylacetone ameliorates ischemic acute renal failure via induction of Hsp70. Kidney Int (2005) 0.93

Post-translational regulation of CND41 protease activity in senescent tobacco leaves. Planta (2005) 0.92

Crystal structures of Escherichia coli gamma-glutamyltranspeptidase in complex with azaserine and acivicin: novel mechanistic implication for inhibition by glutamine antagonists. J Mol Biol (2008) 0.92

Knockdown of berberine bridge enzyme by RNAi accumulates (S)-reticuline and activates a silent pathway in cultured California poppy cells. Transgenic Res (2006) 0.92

Isoquinoline alkaloid biosynthesis is regulated by a unique bHLH-type transcription factor in Coptis japonica. Plant Cell Physiol (2011) 0.91

Molecular cloning and characterization of a cytochrome P450 in sanguinarine biosynthesis from Eschscholzia californica cells. Phytochemistry (2012) 0.91

Free oligosaccharides in the cytosol of Caenorhabditis elegans are generated through endoplasmic reticulum-golgi trafficking. J Biol Chem (2007) 0.91

Changes in microbiota population during fermentation of narezushi as revealed by pyrosequencing analysis. Biosci Biotechnol Biochem (2012) 0.91