Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach.

PubWeight™: 0.85‹?›

🔗 View Article (PMID 15357868)

Published in Proteome Sci on September 09, 2004

Authors

Guennadi A Khoudoli1, Iain M Porter, J Julian Blow, Jason R Swedlow

Author Affiliations

1: Division of Gene Regulation and Expression, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK. g.a.khoudoli@dundee.ac.uk

Articles cited by this

High resolution two-dimensional electrophoresis of proteins. J Biol Chem (1975) 133.45

Geminin, an inhibitor of DNA replication, is degraded during mitosis. Cell (1998) 6.69

Inhibition of eukaryotic DNA replication by geminin binding to Cdt1. Science (2000) 6.48

Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein. Cell (1997) 5.86

A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro. Cell (1994) 5.43

Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin. Nat Cell Biol (2001) 5.37

The making of the mitotic chromosome: modern insights into classical questions. Mol Cell (2003) 2.66

Proteomic analysis of the Escherichia coli outer membrane. Eur J Biochem (2000) 2.59

Chromatin-associated protein phosphatase 1 regulates aurora-B and histone H3 phosphorylation. J Biol Chem (2001) 2.54

Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor. J Cell Biol (1993) 2.44

The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides. EMBO J (1997) 2.27

Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains. Proteomics (2002) 2.24

Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis (1998) 1.88

Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs. EMBO J (1997) 1.83

A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods. Nucleic Acids Res (1994) 1.81

Control of chromosomal DNA replication in the early Xenopus embryo. EMBO J (2001) 1.57

New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis. Electrophoresis (1998) 1.49

The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes. Anal Biochem (1983) 1.28

Reduction and alkylation of proteins in preparation of two-dimensional map analysis: why, when, and how? Electrophoresis (2001) 1.23

Two-dimensional electrophoresis of membrane proteins using immobilized pH gradients. Anal Biochem (2000) 1.13

Sample preparation for two-dimensional gel electrophoresis. Proteomics (2003) 1.06

Application of Taguchi experimental design to the optimisation of a baculovirus expression system. Biochem Soc Trans (1995) 0.94

Taguchi optimisation of ELISA procedures. J Immunol Methods (1999) 0.93

DAF optimization using Taguchi methods and the effect of thermal cycling parameters on DNA amplification. Biotechniques (1998) 0.89

Alkylation kinetics of proteins in preparation for two-dimensional maps: a matrix assisted laser desorption/ionization-mass spectrometry investigation. Electrophoresis (2001) 0.89

Protein alkylation in the presence/absence of thiourea in proteome analysis: a matrix assisted laser desorption/ionization-time of flight-mass spectrometry investigation. Electrophoresis (2001) 0.88

Structure-efficiency relationships of zwitterionic detergents as protein solubilizers in two-dimensional electrophoresis. Proteomics (2003) 0.83

Articles by these authors

Metadata matters: access to image data in the real world. J Cell Biol (2010) 12.49

The Open Microscopy Environment (OME) Data Model and XML file: open tools for informatics and quantitative analysis in biological imaging. Genome Biol (2005) 5.16

Aurora B regulates MCAK at the mitotic centromere. Dev Cell (2004) 4.94

Dormant origins licensed by excess Mcm2-7 are required for human cells to survive replicative stress. Genes Dev (2007) 4.60

OMERO: flexible, model-driven data management for experimental biology. Nat Methods (2012) 3.72

Live-cell imaging reveals replication of individual replicons in eukaryotic replication factories. Cell (2006) 3.56

Cajal body dynamics and association with chromatin are ATP-dependent. Nat Cell Biol (2002) 3.28

Excess Mcm2-7 license dormant origins of replication that can be used under conditions of replicative stress. J Cell Biol (2006) 3.27

Biological imaging software tools. Nat Methods (2012) 2.91

Mitotic mechanics: the auroras come into view. Curr Opin Cell Biol (2003) 2.31

Human CLASP1 is an outer kinetochore component that regulates spindle microtubule dynamics. Cell (2003) 2.20

Regulating the licensing of DNA replication origins in metazoa. Curr Opin Cell Biol (2006) 2.08

To 5D and beyond: quantitative fluorescence microscopy in the postgenomic era. Traffic (2002) 2.04

Cell type-specific responses of human cells to inhibition of replication licensing. Oncogene (2002) 1.91

Evaluating performance in three-dimensional fluorescence microscopy. J Microsc (2007) 1.89

The role of Cdc6 in ensuring complete genome licensing and S phase checkpoint activation. J Cell Biol (2004) 1.89

Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases. J Cell Biol (2010) 1.89

Cdt1 downregulation by proteolysis and geminin inhibition prevents DNA re-replication in Xenopus. EMBO J (2004) 1.84

Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol (2013) 1.81

Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle. Mol Biol Cell (2003) 1.78

Deregulated replication licensing causes DNA fragmentation consistent with head-to-tail fork collision. Mol Cell (2006) 1.73

Characterization of a novel ATR-dependent, Chk1-independent, intra-S-phase checkpoint that suppresses initiation of replication in Xenopus. J Cell Sci (2004) 1.72

Geminin becomes activated as an inhibitor of Cdt1/RLF-B following nuclear import. Curr Biol (2002) 1.63

Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories. J Cell Biol (2010) 1.63

ELYS/MEL-28 chromatin association coordinates nuclear pore complex assembly and replication licensing. Curr Biol (2007) 1.61

MCM2-7 form double hexamers at licensed origins in Xenopus egg extract. J Biol Chem (2011) 1.55

Macromolecular mobility inside the cell nucleus. Trends Cell Biol (2002) 1.53

Mammalian nuclei become licensed for DNA replication during late telophase. J Cell Sci (2002) 1.51

Non-proteolytic inactivation of geminin requires CDK-dependent ubiquitination. Nat Cell Biol (2004) 1.45

Mitotic spindle orientation distinguishes stem cell and terminal modes of neuron production in the early spinal cord. Development (2007) 1.42

Open tools for storage and management of quantitative image data. Methods Cell Biol (2008) 1.39

Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis. J Cell Biol (2010) 1.30

Phosphorylation regulates the dynamic interaction of RCC1 with chromosomes during mitosis. Curr Biol (2004) 1.30

CDC-48/p97 coordinates CDT-1 degradation with GINS chromatin dissociation to ensure faithful DNA replication. Mol Cell (2011) 1.24

The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription. Nucleic Acids Res (2005) 1.22

Bod1, a novel kinetochore protein required for chromosome biorientation. J Cell Biol (2007) 1.22

DNA replication licensing in somatic and germ cells. J Cell Sci (2004) 1.21

Replication factory activation can be decoupled from the replication timing program by modulating Cdk levels. J Cell Biol (2010) 1.20

Data management challenges in three-dimensional EM. Nat Struct Mol Biol (2012) 1.19

Functional domains of the Xenopus replication licensing factor Cdt1. Nucleic Acids Res (2005) 1.16

The Nup107-160 nucleoporin complex promotes mitotic events via control of the localization state of the chromosome passenger complex. Mol Biol Cell (2009) 1.15

Clusters, factories and domains: The complex structure of S-phase comes into focus. Cell Cycle (2010) 1.15

Kinetochores coordinate pericentromeric cohesion and early DNA replication by Cdc7-Dbf4 kinase recruitment. Mol Cell (2013) 1.14

A novel reporter of notch signalling indicates regulated and random Notch activation during vertebrate neurogenesis. BMC Biol (2011) 1.14

PHD1 links cell-cycle progression to oxygen sensing through hydroxylation of the centrosomal protein Cep192. Dev Cell (2013) 1.12

The dynamics of replication licensing in live Caenorhabditis elegans embryos. J Cell Biol (2012) 1.11

A Xenopus Dbf4 homolog is required for Cdc7 chromatin binding and DNA replication. BMC Mol Biol (2004) 1.06

Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition. Curr Biol (2008) 1.04

Rapid induction of pluripotency genes after exposure of human somatic cells to mouse ES cell extracts. Exp Cell Res (2008) 1.03

Phosphorylation by aurora-B negatively regulates survivin function during mitosis. Cell Cycle (2007) 1.02

Re-replication induced by geminin depletion occurs from G2 and is enhanced by checkpoint activation. J Cell Sci (2012) 1.02

The elusive determinants of replication origins. EMBO Rep (2007) 1.01

Deregulated origin licensing leads to chromosomal breaks by rereplication of a gapped DNA template. Genes Dev (2013) 1.00

Quantitative analysis of digital microscope images. Methods Cell Biol (2007) 1.00

Dormant origins, the licensing checkpoint, and the response to replicative stresses. Cold Spring Harb Perspect Biol (2012) 0.99

Replisome stall events have shaped the distribution of replication origins in the genomes of yeasts. Nucleic Acids Res (2013) 0.99

Bod1 regulates protein phosphatase 2A at mitotic kinetochores. Nat Commun (2013) 0.97

PTIP/Swift is required for efficient PCNA ubiquitination in response to DNA damage. DNA Repair (Amst) (2008) 0.95

Web-based visualisation and analysis of 3D electron-microscopy data from EMDB and PDB. J Struct Biol (2013) 0.93

Dynamic interactions of high Cdt1 and geminin levels regulate S phase in early Xenopus embryos. Development (2011) 0.92

Preparation and use of Xenopus egg extracts to study DNA replication and chromatin associated proteins. Methods (2012) 0.92

OMX: a new platform for multimodal, multichannel wide-field imaging. Cold Spring Harb Protoc (2011) 0.91

How to be a mitotic chromosome. Chromosome Res (2011) 0.87

High-resolution live imaging of cell behavior in the developing neuroepithelium. J Vis Exp (2012) 0.87

The licensing checkpoint opens up. Cell Cycle (2009) 0.86

Stochastic association of neighboring replicons creates replication factories in budding yeast. J Cell Biol (2013) 0.85

Histone acetylation by HBO1 tightens replication licensing. Mol Cell (2010) 0.85

Xenopus Cdc7 executes its essential function early in S phase and is counteracted by checkpoint-regulated protein phosphatase 1. Open Biol (2014) 0.85

Phosphorylated DegU manipulates cell fate differentiation in the Bacillus subtilis biofilm. J Bacteriol (2013) 0.84

Mcm8 and Mcm9 form a dimeric complex in Xenopus laevis egg extract that is not essential for DNA replication initiation. Cell Cycle (2013) 0.83

RNA polymerase II transcription in living color. Nat Struct Mol Biol (2007) 0.83

Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication. Biochim Biophys Acta (2011) 0.81

The role of the replication licensing system in cell proliferation and cancer. Prog Cell Cycle Res (2003) 0.81

Negative regulation of geminin by CDK-dependent ubiquitination controls replication licensing. Cell Cycle (2004) 0.81

Software tools, data structures, and interfaces for microscope imaging. Cold Spring Harb Protoc (2012) 0.81

The Geminin and Idas coiled coils preferentially form a heterodimer that inhibits Geminin function in DNA replication licensing. J Biol Chem (2013) 0.81

Optimal placement of origins for DNA replication. Phys Rev Lett (2012) 0.81

The adenomatous polyposis coli protein contributes to normal compaction of mitotic chromatin. PLoS One (2012) 0.80

A role for dormant origins in tumor suppression. Mol Cell (2011) 0.79

Evidence for a mammalian late-G1 phase inhibitor of replication licensing distinct from geminin or Cdk activity. Nucleus (2011) 0.77

Cell Cycle Synchronization in Xenopus Egg Extracts. Methods Mol Biol (2016) 0.77

Quantitative analysis of digital microscope images. Methods Cell Biol (2013) 0.76

Degradation ensures integrity. Nature (2003) 0.76

Cell biology forum: Genome-wide view of mitosis. Nature (2010) 0.75

In vivo imaging of mammalian cells: cell engineering and viability. Cold Spring Harb Protoc (2009) 0.75

In vivo imaging of mammalian cells: image acquisition and analysis. Cold Spring Harb Protoc (2009) 0.75

Chromosome condensation: DNA compaction in real time. Curr Biol (2004) 0.75

Erratum: Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans. Nat Commun (2015) 0.75

Building a scientific data grid with DiGS. Philos Trans A Math Phys Eng Sci (2009) 0.75