Published in Mol Biol Cell on October 06, 2004
Bioorthogonal chemistry: fishing for selectivity in a sea of functionality. Angew Chem Int Ed Engl (2009) 8.15
Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells. Chem Rev (2011) 1.88
Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH. Nat Chem Biol (2007) 1.80
Tunable and reversible drug control of protein production via a self-excising degron. Nat Chem Biol (2015) 1.52
Fluorescent labeling of tetracysteine-tagged proteins in intact cells. Nat Protoc (2010) 1.28
Chemical tags for labeling proteins inside living cells. Acc Chem Res (2011) 1.26
Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae. Yeast (2009) 1.20
Filament formation by metabolic enzymes is a specific adaptation to an advanced state of cellular starvation. Elife (2014) 1.17
Tracking bio-molecules in live cells using quantum dots. J Biophotonics (2008) 1.08
Surveying protein structure and function using bis-arsenical small molecules. Acc Chem Res (2011) 1.04
Endothelin receptor dimers evaluated by FRET, ligand binding, and calcium mobilization. Biophys J (2008) 1.01
Imaging of membrane systems and membrane traffic in living cells. Cold Spring Harb Protoc (2011) 1.00
SNAP-, CLIP- and Halo-tag labelling of budding yeast cells. PLoS One (2013) 1.00
Recent advances in fluorescent labeling techniques for fluorescence microscopy. Acta Histochem Cytochem (2007) 0.95
Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast. Yeast (2012) 0.95
Site-specific orthogonal labeling of the carboxy terminus of alpha-tubulin. ACS Chem Biol (2010) 0.93
Accumulation of FlAsH/Lumio Green in active mitochondria can be reversed by beta-mercaptoethanol for specific staining of tetracysteine-tagged proteins. Histochem Cell Biol (2006) 0.92
The m-AAA protease processes cytochrome c peroxidase preferentially at the inner boundary membrane of mitochondria. Mol Biol Cell (2008) 0.88
Design and use of fluorescent fusion proteins in cell biology. Curr Protoc Cell Biol (2005) 0.86
Tracking the biogenesis and inheritance of subpellicular microtubule in Trypanosoma brucei with inducible YFP-α-tubulin. Biomed Res Int (2014) 0.81
Advances in chemical labeling of proteins in living cells. Cell Tissue Res (2015) 0.80
High-affinity fluorescent ligands for the 5-HT(3) receptor. Bioorg Med Chem Lett (2011) 0.79
Interactions of AsCy3 with cysteine-rich peptides. Org Lett (2014) 0.79
Fluorogenic Labeling Strategies for Biological Imaging. Int J Mol Sci (2017) 0.76
Imaging the boundaries-innovative tools for microscopy of living cells and real-time imaging. J Chem Biol (2008) 0.76
Selective Chemical Labeling of Proteins with Small Fluorescent Molecules Based on Metal-Chelation Methodology. Sensors (Basel) (2008) 0.76
Tracking single proteins in live cells using single-chain antibody fragment-fluorescent quantum dot affinity pair. Methods Enzymol (2010) 0.76
Synthesis of non-linear protein dimers through a genetically encoded Thiol-ene reaction. PLoS One (2014) 0.75
Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs). Proteome Sci (2017) 0.75
Characterization of Fluorescein Arsenical Hairpin (FlAsH) as a Probe for Single-Molecule Fluorescence Spectroscopy. Sci Rep (2017) 0.75
Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res (1992) 34.33
The green fluorescent protein. Annu Rev Biochem (1998) 34.17
Global analysis of protein localization in budding yeast. Nature (2003) 33.22
Specific covalent labeling of recombinant protein molecules inside live cells. Science (1998) 9.32
High-resolution model of the microtubule. Cell (1999) 9.04
Getting started with yeast. Methods Enzymol (2002) 6.60
New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications. J Am Chem Soc (2002) 5.83
Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast (1997) 5.61
Multicolor and electron microscopic imaging of connexin trafficking. Science (2002) 5.60
Mitosis in living budding yeast: anaphase A but no metaphase plate. Science (1997) 5.15
Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex. J Cell Biol (1997) 5.08
The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae. Nat Cell Biol (2000) 3.76
Mitochondria-targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae. Yeast (2000) 3.52
Mechanism of the single-headed processivity: diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin. Proc Natl Acad Sci U S A (2000) 2.73
The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. Nat Biotechnol (2004) 2.48
Fluorescent labeling of recombinant proteins in living cells with FlAsH. Methods Enzymol (2000) 2.24
Genetically targeted chromophore-assisted light inactivation. Nat Biotechnol (2003) 1.90
Mechanisms of protein import into mitochondria. Curr Biol (2003) 1.76
The protein-labeling reagent FLASH-EDT2 binds not only to CCXXCC motifs but also non-specifically to endogenous cysteine-rich proteins. Pflugers Arch (2001) 1.71
E-MAP-115 (ensconsin) associates dynamically with microtubules in vivo and is not a physiological modulator of microtubule dynamics. J Cell Sci (1999) 1.69
Transgenically encoded protein photoinactivation (FlAsH-FALI): acute inactivation of synaptotagmin I. Neuron (2002) 1.65
A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae. Gene (2000) 1.30
In vitro and in vivo nucleotide exchange directed by chimeric RNA/DNA oligonucleotides in Saccharomyces cerevisae. Mol Microbiol (2001) 1.12
The potential of nucleic acid repair in functional genomics. Nat Biotechnol (2001) 0.95
Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. Proc Natl Acad Sci U S A (2005) 6.65
Fluorescence nanoscopy by ground-state depletion and single-molecule return. Nat Methods (2008) 4.69
Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters. Biophys J (2007) 3.93
Spherical nanosized focal spot unravels the interior of cells. Nat Methods (2008) 3.43
Nanoscale resolution in GFP-based microscopy. Nat Methods (2006) 3.19
Diffraction-unlimited all-optical imaging and writing with a photochromic GFP. Nature (2011) 2.56
Two-color far-field fluorescence nanoscopy. Biophys J (2007) 2.44
1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants. Biochem J (2007) 2.33
Structure and mechanism of the reversible photoswitch of a fluorescent protein. Proc Natl Acad Sci U S A (2005) 2.21
Fast 100-nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast. Proc Natl Acad Sci U S A (2002) 2.19
Structural basis for reversible photoswitching in Dronpa. Proc Natl Acad Sci U S A (2007) 2.08
Super-resolution microscopy reveals that mammalian mitochondrial nucleoids have a uniform size and frequently contain a single copy of mtDNA. Proc Natl Acad Sci U S A (2011) 1.90
Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy. Nat Biotechnol (2008) 1.80
Concepts for nanoscale resolution in fluorescence microscopy. Curr Opin Neurobiol (2004) 1.75
Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength. Biophys J (2010) 1.74
A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching. Nat Biotechnol (2011) 1.74
Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy. Biophys J (2008) 1.67
rsEGFP2 enables fast RESOLFT nanoscopy of living cells. Elife (2012) 1.61
Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching. Microsc Res Tech (2007) 1.58
Stimulated emission depletion nanoscopy of living cells using SNAP-tag fusion proteins. Biophys J (2010) 1.52
Nanoscopy with more than 100,000 'doughnuts'. Nat Methods (2013) 1.48
Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex. Cell Metab (2012) 1.48
Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane. Mol Syst Biol (2011) 1.44
Immunofluorescence stimulated emission depletion microscopy. Nat Biotechnol (2003) 1.38
MINOS1 is a conserved component of mitofilin complexes and required for mitochondrial function and cristae organization. Mol Biol Cell (2011) 1.37
Nanoscopy of living brain slices with low light levels. Neuron (2012) 1.35
Uniform nomenclature for the mitochondrial contact site and cristae organizing system. J Cell Biol (2014) 1.34
Mdm31 and Mdm32 are inner membrane proteins required for maintenance of mitochondrial shape and stability of mitochondrial DNA nucleoids in yeast. J Cell Biol (2005) 1.33
Mitochondrial cristae revealed with focused light. Nano Lett (2009) 1.32
The class V myosin motor protein, Myo2, plays a major role in mitochondrial motility in Saccharomyces cerevisiae. J Cell Biol (2008) 1.31
STED super-resolution microscopy reveals an array of MINOS clusters along human mitochondria. Proc Natl Acad Sci U S A (2013) 1.29
Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient. Proc Natl Acad Sci U S A (2011) 1.27
The inner membrane protein Mdm33 controls mitochondrial morphology in yeast. J Cell Biol (2003) 1.22
Differential protein distributions define two sub-compartments of the mitochondrial inner membrane in yeast. FEBS Lett (2006) 1.18
Sample preparation for STED microscopy. Methods Mol Biol (2010) 1.14
Fis1p and Caf4p, but not Mdv1p, determine the polar localization of Dnm1p clusters on the mitochondrial surface. J Cell Sci (2006) 1.13
Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy. Biophys J (2004) 1.12
Rhodamines NN: a novel class of caged fluorescent dyes. Angew Chem Int Ed Engl (2010) 1.09
Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms. PMC Biophys (2010) 1.01
SNAP-, CLIP- and Halo-tag labelling of budding yeast cells. PLoS One (2013) 1.00
Dual-label STED nanoscopy of living cells using photochromism. Nano Lett (2011) 1.00
Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron. J Biol Chem (2010) 0.99
New fluorinated rhodamines for optical microscopy and nanoscopy. Chemistry (2010) 0.98
Nanoscale separation of molecular species based on their rotational mobility. Opt Express (2008) 0.95
Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins. Chemphyschem (2014) 0.88
The m-AAA protease processes cytochrome c peroxidase preferentially at the inner boundary membrane of mitochondria. Mol Biol Cell (2008) 0.88
Far-field autofluorescence nanoscopy. Nano Lett (2010) 0.86
Coordinate-targeted and coordinate-stochastic super-resolution microscopy with the reversibly switchable fluorescent protein Dreiklang. Chemphyschem (2014) 0.82
Red-emitting rhodamines with hydroxylated, sulfonated, and phosphorylated dye residues and their use in fluorescence nanoscopy. Chemistry (2012) 0.82
4Pi microscopy of quantum dot-labeled cellular structures. J Struct Biol (2006) 0.81
The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates. Mol Biol Cell (2012) 0.81
Mixed oxide coatings for optics. Appl Opt (2011) 0.75