Published in Nat Methods on February 17, 2005
Bioorthogonal chemistry: fishing for selectivity in a sea of functionality. Angew Chem Int Ed Engl (2009) 8.15
G protein coupled receptor structure and activation. Biochim Biophys Acta (2006) 2.48
G protein-coupled receptors sense fluid shear stress in endothelial cells. Proc Natl Acad Sci U S A (2006) 2.21
Dynamics of receptor/G protein coupling in living cells. EMBO J (2005) 2.06
Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering. Protein Sci (2005) 1.94
Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells. Chem Rev (2011) 1.88
An uncoupling channel within the c-subunit ring of the F1FO ATP synthase is the mitochondrial permeability transition pore. Proc Natl Acad Sci U S A (2014) 1.85
Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH. Nat Chem Biol (2007) 1.80
Visualizing biochemical activities in living cells. Nat Chem Biol (2009) 1.49
Fluorescence changes reveal kinetic steps of muscarinic receptor-mediated modulation of phosphoinositides and Kv7.2/7.3 K+ channels. J Gen Physiol (2009) 1.45
Real-time optical recording of beta1-adrenergic receptor activation reveals supersensitivity of the Arg389 variant to carvedilol. J Clin Invest (2007) 1.44
G-protein-coupled receptors: past, present and future. Br J Pharmacol (2006) 1.41
Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins. Nat Protoc (2008) 1.31
Kinetics of M1 muscarinic receptor and G protein signaling to phospholipase C in living cells. J Gen Physiol (2010) 1.29
Cell-surface sensors for real-time probing of cellular environments. Nat Nanotechnol (2011) 1.28
Patch-clamp coordinated spectroscopy shows P2X2 receptor permeability dynamics require cytosolic domain rearrangements but not Panx-1 channels. Proc Natl Acad Sci U S A (2008) 1.28
Fluorescent labeling of tetracysteine-tagged proteins in intact cells. Nat Protoc (2010) 1.28
Shining light on signaling and metabolic networks by genetically encoded biosensors. Curr Opin Plant Biol (2005) 1.25
Conformational changes in G-protein-coupled receptors-the quest for functionally selective conformations is open. Br J Pharmacol (2007) 1.23
Visualizing flock house virus infection in Drosophila cells with correlated fluorescence and electron microscopy. J Struct Biol (2007) 1.21
Differential signaling of the endogenous agonists at the beta2-adrenergic receptor. J Biol Chem (2010) 1.20
Studying the dynamics of flagella in multicellular communities of Escherichia coli by using biarsenical dyes. Appl Environ Microbiol (2009) 1.17
Imaging proteins inside cells with fluorescent tags. Trends Biotechnol (2011) 1.17
Hairpin structure of a biarsenical-tetracysteine motif determined by NMR spectroscopy. J Am Chem Soc (2009) 1.16
Fluorescence applications in molecular neurobiology. Neuron (2010) 1.13
Kinetics of G-protein-coupled receptor signals in intact cells. Br J Pharmacol (2008) 1.12
The conformational signature of β-arrestin2 predicts its trafficking and signalling functions. Nature (2016) 1.11
Both ligand- and cell-specific parameters control ligand agonism in a kinetic model of g protein-coupled receptor signaling. PLoS Comput Biol (2007) 1.08
Tracking bio-molecules in live cells using quantum dots. J Biophotonics (2008) 1.08
The allosteric vestibule of a seven transmembrane helical receptor controls G-protein coupling. Nat Commun (2012) 1.05
Surveying protein structure and function using bis-arsenical small molecules. Acc Chem Res (2011) 1.04
Single-cell analysis of G-protein signal transduction. J Biol Chem (2015) 1.04
Quantitative imaging for discovery and assembly of the metabo-regulome. New Phytol (2008) 1.03
Endosomal generation of cAMP in GPCR signaling. Nat Chem Biol (2014) 1.03
Endothelin receptor dimers evaluated by FRET, ligand binding, and calcium mobilization. Biophys J (2008) 1.01
Trafficking and recycling of the connexin43 gap junction protein during mitosis. Traffic (2010) 1.01
Developing chemical genetic approaches to explore G protein-coupled receptor function: validation of the use of a receptor activated solely by synthetic ligand (RASSL). Mol Pharmacol (2011) 1.00
Picking faces out of a crowd: genetic labels for identification of proteins in correlated light and electron microscopy imaging. Methods Cell Biol (2012) 0.97
Systematic definition of protein constituents along the major polarization axis reveals an adaptive reuse of the polarization machinery in pheromone-treated budding yeast. J Proteome Res (2009) 0.96
Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells. J Biol Chem (2011) 0.95
Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast. Yeast (2012) 0.95
Deep brain optical measurements of cell type-specific neural activity in behaving mice. Nat Protoc (2014) 0.95
Structure of the Ca2+-dependent PP2A heterotrimer and insights into Cdc6 dephosphorylation. Cell Res (2013) 0.93
Optical measurement of mGluR1 conformational changes reveals fast activation, slow deactivation, and sensitization. Proc Natl Acad Sci U S A (2009) 0.92
Illuminating the life of GPCRs. Cell Commun Signal (2009) 0.92
A stilbene that binds selectively to transthyretin in cells and remains dark until it undergoes a chemoselective reaction to create a bright blue fluorescent conjugate. J Am Chem Soc (2010) 0.92
Hallucinogen actions on 5-HT receptors reveal distinct mechanisms of activation and signaling by G protein-coupled receptors. AAPS J (2006) 0.91
Molecular Biomechanics: The Molecular Basis of How Forces Regulate Cellular Function. Mol Cell Biomech (2010) 0.91
Analysis of membrane-localized binding kinetics with FRAP. Eur Biophys J (2008) 0.91
β-Arrestin biosensors reveal a rapid, receptor-dependent activation/deactivation cycle. Nature (2016) 0.91
Comparison between whole distribution- and average-based approaches to the determination of fluorescence resonance energy transfer efficiency in ensembles of proteins in living cells. Biophys J (2010) 0.90
A fluorescence resonance energy transfer-based M2 muscarinic receptor sensor reveals rapid kinetics of allosteric modulation. J Biol Chem (2010) 0.90
Green light to illuminate signal transduction events. Trends Cell Biol (2009) 0.89
Monitoring a coordinated exchange process in a four-component biological interaction system: development of a time-resolved terbium-based one-donor/three-acceptor multicolor FRET system. J Am Chem Soc (2010) 0.88
Brought to life: targeted activation of enzyme function with small molecules. J Chem Biol (2008) 0.88
Adenosine A(2A) receptor dynamics studied with the novel fluorescent agonist Alexa488-APEC. Eur J Pharmacol (2008) 0.87
Time-resolved fluorescence ligand binding for G protein-coupled receptors. Nat Protoc (2013) 0.87
Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors. Biochim Biophys Acta (2013) 0.86
Cell-based reporters reveal in vivo dynamics of dopamine and norepinephrine release in murine cortex. Nat Methods (2014) 0.85
PP2A as a master regulator of the cell cycle. Crit Rev Biochem Mol Biol (2016) 0.85
Cross-talk between carboxypeptidase M and the kinin B1 receptor mediates a new mode of G protein-coupled receptor signaling. J Biol Chem (2011) 0.85
Theme and variations on kinetics of GPCR activation/deactivation. J Recept Signal Transduct Res (2010) 0.84
FACS-based selection of tandem tetracysteine peptides with improved ReAsH brightness in live cells. Chembiochem (2010) 0.84
Ligand-induced rearrangements of the GABA(B) receptor revealed by fluorescence resonance energy transfer. J Biol Chem (2010) 0.84
Unraveling the structure and function of G protein-coupled receptors through NMR spectroscopy. Curr Pharm Des (2009) 0.84
Fluorescence dequenching makes haem-free soluble guanylate cyclase detectable in living cells. PLoS One (2011) 0.83
Utilization of extracellular information before ligand-receptor binding reaches equilibrium expands and shifts the input dynamic range. Proc Natl Acad Sci U S A (2014) 0.83
Rotational diffusion of the α(2a) adrenergic receptor revealed by FlAsH labeling in living cells. Biophys J (2011) 0.83
From PII signaling to metabolite sensing: a novel 2-oxoglutarate sensor that details PII-NAGK complex formation. PLoS One (2013) 0.83
D2-like dopamine and β-adrenergic receptors form a signaling complex that integrates Gs- and Gi-mediated regulation of adenylyl cyclase. Cell Signal (2012) 0.82
Mapping membrane protein structure with fluorescence. Curr Opin Struct Biol (2012) 0.82
Engineering genetically encoded FRET sensors. Sensors (Basel) (2014) 0.82
Purines as potential morphogens during embryonic development. Purinergic Signal (2012) 0.82
Biarsenical-tetracysteine motif as a fluorescent tag for detection in capillary electrophoresis. Anal Chem (2008) 0.82
Rhodopsin photointermediates in two-dimensional crystals at physiological temperatures. Biochemistry (2006) 0.81
Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics by guanine nucleotides. Anal Biochem (2007) 0.81
Studying ligand efficacy at G protein-coupled receptors using FRET. Methods Mol Biol (2011) 0.81
Bifunctional coumarin derivatives that inhibit transthyretin amyloidogenesis and serve as fluorescent transthyretin folding sensors. Chem Commun (Camb) (2013) 0.80
Conformational changes in the parathyroid hormone receptor associated with activation by agonist. Mol Endocrinol (2008) 0.80
Advances in chemical labeling of proteins in living cells. Cell Tissue Res (2015) 0.80
Dynamic fluorescence depolarization: a powerful tool to explore protein folding on the ribosome. Methods (2010) 0.80
Uncovering caffeine's adenosine A2A receptor inverse agonism in experimental parkinsonism. ACS Chem Biol (2014) 0.80
Optical probes based on G protein-coupled receptors - added work or added value? Br J Pharmacol (2015) 0.80
Carboxypeptidase M is a positive allosteric modulator of the kinin B1 receptor. J Biol Chem (2013) 0.79
FRET-based detection of M1 muscarinic acetylcholine receptor activation by orthosteric and allosteric agonists. PLoS One (2012) 0.79
Interactions of AsCy3 with cysteine-rich peptides. Org Lett (2014) 0.79
A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells. PLoS One (2016) 0.78
Activation biosensor for G protein-coupled receptors: a FRET-based m1 muscarinic activation sensor that regulates G(q). PLoS One (2012) 0.78
Conformational detection of p53's oligomeric state by FlAsH Fluorescence. Biochem Biophys Res Commun (2009) 0.78
Inntags: small self-structured epitopes for innocuous protein tagging. Nat Methods (2015) 0.78
Fluorescence resonance energy transfer-based intracellular assay for the conformation of hepatitis C virus drug target NS5A. J Virol (2012) 0.78
Micelle-Enhanced Bioorthogonal Labeling of Genetically Encoded Azido Groups on the Lipid-Embedded Surface of a GPCR. Chembiochem (2015) 0.78
Intramolecular fluorescence resonance energy transfer (FRET) sensors of the orexin OX1 and OX2 receptors identify slow kinetics of agonist activation. J Biol Chem (2012) 0.78
Mechanosensory Signaling in Enterochromaffin Cells and 5-HT Release: Potential Implications for Gut Inflammation. Front Neurosci (2016) 0.78
Carboxypeptidase M augments kinin B1 receptor signaling by conformational crosstalk and enhances endothelial nitric oxide output. Biol Chem (2013) 0.77
Fluorogenic Labeling Strategies for Biological Imaging. Int J Mol Sci (2017) 0.76
Visualization of Protein Interactions in Living Cells. Self Nonself (2011) 0.76
Imaging the boundaries-innovative tools for microscopy of living cells and real-time imaging. J Chem Biol (2008) 0.76
New eyes to see texture in ligand efficacy. Nat Methods (2005) 0.76
Analyses of the effects of Gq protein on the activated states of the muscarinic M3 receptor and the purinergic P2Y1 receptor. Physiol Rep (2013) 0.76
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol (2004) 36.25
A monomeric red fluorescent protein. Proc Natl Acad Sci U S A (2002) 21.56
Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science (2002) 18.89
A guide to choosing fluorescent proteins. Nat Methods (2005) 18.55
The fluorescent toolbox for assessing protein location and function. Science (2006) 14.02
Creating new fluorescent probes for cell biology. Nat Rev Mol Cell Biol (2002) 9.25
STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature (2005) 8.96
Improving the photostability of bright monomeric orange and red fluorescent proteins. Nat Methods (2008) 8.41
Bid, Bax, and lipids cooperate to form supramolecular openings in the outer mitochondrial membrane. Cell (2002) 8.38
New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications. J Am Chem Soc (2002) 5.83
Multicolor and electron microscopic imaging of connexin trafficking. Science (2002) 5.60
Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. J Biol Chem (2004) 5.26
Protoplasmic astrocytes in CA1 stratum radiatum occupy separate anatomical domains. J Neurosci (2002) 5.14
Novel single chain cAMP sensors for receptor-induced signal propagation. J Biol Chem (2004) 5.00
Tumor imaging by means of proteolytic activation of cell-penetrating peptides. Proc Natl Acad Sci U S A (2004) 4.95
Characterization of engineered channelrhodopsin variants with improved properties and kinetics. Biophys J (2009) 4.75
Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators. J Biol Chem (2004) 4.75
Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. Science (2009) 4.67
Novel chromophores and buried charges control color in mFruits. Biochemistry (2006) 4.64
Visualizing the mechanical activation of Src. Nature (2005) 4.63
Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol (2006) 4.52
Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A (2004) 4.46
Nitric oxide-induced mitochondrial fission is regulated by dynamin-related GTPases in neurons. EMBO J (2006) 4.43
A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol (2003) 4.38
Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc Natl Acad Sci U S A (2004) 4.37
Dedifferentiation of neurons and astrocytes by oncogenes can induce gliomas in mice. Science (2012) 4.14
E-neuroscience: challenges and triumphs in integrating distributed data from molecules to brains. Nat Neurosci (2004) 4.13
A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. PLoS Biol (2011) 4.07
Measuring calcium signaling using genetically targetable fluorescent indicators. Nat Protoc (2006) 3.96
Sestrin as a feedback inhibitor of TOR that prevents age-related pathologies. Science (2010) 3.86
Spatial mapping of integrin interactions and dynamics during cell migration by image correlation microscopy. J Cell Sci (2004) 3.70
Activity-dependent regulation of dendritic synthesis and trafficking of AMPA receptors. Nat Neurosci (2004) 3.64
Time-restricted feeding without reducing caloric intake prevents metabolic diseases in mice fed a high-fat diet. Cell Metab (2012) 3.64
Improving FRET dynamic range with bright green and red fluorescent proteins. Nat Methods (2012) 3.54
Disruption of mitochondrial function during apoptosis is mediated by caspase cleavage of the p75 subunit of complex I of the electron transport chain. Cell (2004) 3.49
Surgery with molecular fluorescence imaging using activatable cell-penetrating peptides decreases residual cancer and improves survival. Proc Natl Acad Sci U S A (2010) 3.41
Synapse formation on neurons born in the adult hippocampus. Nat Neurosci (2007) 3.33
Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity. Nat Biotechnol (2005) 3.28
Imaging tri-fusion multimodality reporter gene expression in living subjects. Cancer Res (2004) 3.16
Biomedical informatics research network: building a national collaboratory to hasten the derivation of new understanding and treatment of disease. Stud Health Technol Inform (2005) 3.14
Autofluorescent proteins with excitation in the optical window for intravital imaging in mammals. Chem Biol (2009) 3.11
Fluorescence-guided surgery with live molecular navigation--a new cutting edge. Nat Rev Cancer (2013) 3.08
Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases. Proc Natl Acad Sci U S A (2010) 3.03
Sustained cyclic AMP production by parathyroid hormone receptor endocytosis. Nat Chem Biol (2009) 3.03
The cell centered database project: an update on building community resources for managing and sharing 3D imaging data. J Struct Biol (2007) 3.00
Beta2-adrenergic receptor redistribution in heart failure changes cAMP compartmentation. Science (2010) 2.99
ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation. Nat Neurosci (2013) 2.98
Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Nat Biotechnol (2012) 2.98
Three-dimensional analysis of a viral RNA replication complex reveals a virus-induced mini-organelle. PLoS Biol (2007) 2.85
Measurement of the millisecond activation switch of G protein-coupled receptors in living cells. Nat Biotechnol (2003) 2.84
Evidence for ectopic neurotransmission at a neuronal synapse. Science (2005) 2.81
Widespread receptivity to neuropeptide PDF throughout the neuronal circadian clock network of Drosophila revealed by real-time cyclic AMP imaging. Neuron (2008) 2.73
Cyclic AMP imaging in adult cardiac myocytes reveals far-reaching beta1-adrenergic but locally confined beta2-adrenergic receptor-mediated signaling. Circ Res (2006) 2.66
Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots. Nat Methods (2005) 2.66
The Arg451Cys-neuroligin-3 mutation associated with autism reveals a defect in protein processing. J Neurosci (2004) 2.66
International Union of Basic and Clinical Pharmacology. LXVII. Recommendations for the recognition and nomenclature of G protein-coupled receptor heteromultimers. Pharmacol Rev (2007) 2.62
Persistent cAMP-signals triggered by internalized G-protein-coupled receptors. PLoS Biol (2009) 2.59
Fluorescence resonance energy transfer-based analysis of cAMP dynamics in live neonatal rat cardiac myocytes reveals distinct functions of compartmentalized phosphodiesterases. Circ Res (2004) 2.58
The cell-centered database: a database for multiscale structural and protein localization data from light and electron microscopy. Neuroinformatics (2003) 2.55
Insulin disrupts beta-adrenergic signalling to protein kinase A in adipocytes. Nature (2005) 2.54
Gi protein activation in intact cells involves subunit rearrangement rather than dissociation. Proc Natl Acad Sci U S A (2003) 2.52
Conformational cross-talk between alpha2A-adrenergic and mu-opioid receptors controls cell signaling. Nat Chem Biol (2008) 2.51
Three-dimensional electron microscopy reveals new details of membrane systems for Ca2+ signaling in the heart. J Cell Sci (2009) 2.49
Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator. Nat Chem Biol (2007) 2.47
JNK1 is required for maintenance of neuronal microtubules and controls phosphorylation of microtubule-associated proteins. Dev Cell (2003) 2.45
Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor. Nat Methods (2008) 2.44
Building a new conceptual framework for receptor heteromers. Nat Chem Biol (2009) 2.41
Protein kinase C switches the Raf kinase inhibitor from Raf-1 to GRK-2. Nature (2003) 2.38
Optically monitoring voltage in neurons by photo-induced electron transfer through molecular wires. Proc Natl Acad Sci U S A (2012) 2.35
Correlated three-dimensional light and electron microscopy reveals transformation of mitochondria during apoptosis. Nat Cell Biol (2007) 2.34
All-optical histology using ultrashort laser pulses. Neuron (2003) 2.33
BI-1 regulates an apoptosis pathway linked to endoplasmic reticulum stress. Mol Cell (2004) 2.32
In vivo characterization of activatable cell penetrating peptides for targeting protease activity in cancer. Integr Biol (Camb) (2009) 2.30
Opa1-mediated cristae opening is Bax/Bak and BH3 dependent, required for apoptosis, and independent of Bak oligomerization. Mol Cell (2008) 2.26
Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins. Biochemistry (2007) 2.23
Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control. PLoS Biol (2004) 2.20
Crosstalk between nitric oxide and zinc pathways to neuronal cell death involving mitochondrial dysfunction and p38-activated K+ channels. Neuron (2004) 2.17
Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Neuron (2013) 2.16
Overview of the Alliance for Cellular Signaling. Nature (2002) 2.15
Feedback inhibition of catecholamine release by two different alpha2-adrenoceptor subtypes prevents progression of heart failure. Circulation (2002) 2.10
Dynamics of receptor/G protein coupling in living cells. EMBO J (2005) 2.06
Analysis of receptor oligomerization by FRAP microscopy. Nat Methods (2009) 2.06
Aptamers switch on fluorescence of triphenylmethane dyes. J Am Chem Soc (2003) 2.05