Published in Proc Natl Acad Sci U S A on May 13, 2005
Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand binding. Chem Rev (2008) 2.27
Structural basis for the aminoacid composition of proteins from halophilic archea. PLoS Biol (2009) 1.27
Function and biotechnology of extremophilic enzymes in low water activity. Aquat Biosyst (2012) 1.03
An antibiotic-resistance enzyme from a deep-sea bacterium. J Am Chem Soc (2010) 0.91
Salinity tolerance in plants. Quantitative approach to ion transport starting from halophytes and stepping to genetic and protein engineering for manipulating ion fluxes. Front Plant Sci (2015) 0.90
Halophilic enzyme activation induced by salts. Sci Rep (2011) 0.89
Intrinsic halotolerance of the psychrophilic alpha-amylase from Pseudoalteromonas haloplanktis. Extremophiles (2007) 0.85
Structural features that govern enzymatic activity in carbonic anhydrase from a low-temperature adapted fish, Chionodraco hamatus. Biophys J (2007) 0.84
Molecular Mechanisms of Enzyme Activation by Monovalent Cations. J Biol Chem (2016) 0.79
The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis. J Appl Phycol (2014) 0.77
The most recently discovered carbonic anhydrase, CA XV, is expressed in the thick ascending limb of Henle and in the collecting ducts of mouse kidney. PLoS One (2010) 0.76
Characterization of a NaCl-tolerant β-N-acetylglucosaminidase from Sphingobacterium sp. HWLB1. Extremophiles (2016) 0.76
Rational engineering of a mesohalophilic carbonic anhydrase to an extreme halotolerant biocatalyst. Nat Commun (2015) 0.76
Distinctive molecular and biochemical characteristics of a glycoside hydrolase family 20 β-N-acetylglucosaminidase and salt tolerance. BMC Biotechnol (2017) 0.75
A Shinella β-N-acetylglucosaminidase of glycoside hydrolase family 20 displays novel biochemical and molecular characteristics. Extremophiles (2017) 0.75
Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr (1998) 169.28
Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features. Biopolymers (1983) 99.69
SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis (1997) 57.27
Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins (1991) 36.69
XtalView/Xfit--A versatile program for manipulating atomic coordinates and electron density. J Struct Biol (1999) 17.84
Classical electrostatics in biology and chemistry. Science (1995) 17.26
Electrostatic recognition between superoxide and copper, zinc superoxide dismutase. Nature (1984) 2.36
Halophilic adaptation of enzymes. Extremophiles (2000) 2.16
An electrostatic mechanism for substrate guidance down the aromatic gorge of acetylcholinesterase. Proc Natl Acad Sci U S A (1993) 2.14
Halophilic enzymes: proteins with a grain of salt. Biophys Chem (2000) 1.96
Structural features that stabilize halophilic malate dehydrogenase from an archaebacterium. Science (1995) 1.95
Elucidation of factors responsible for enhanced thermal stability of proteins: a structural genomics based study. Biochemistry (2002) 1.80
Molecular dissection of Na+ binding to thrombin. J Biol Chem (2004) 1.72
Insights into protein adaptation to a saturated salt environment from the crystal structure of a halophilic 2Fe-2S ferredoxin. Nat Struct Biol (1996) 1.58
Electrostatic contributions to the stability of halophilic proteins. J Mol Biol (1998) 1.42
Crystal structure of the dimeric extracellular domain of human carbonic anhydrase XII, a bitopic membrane protein overexpressed in certain cancer tumor cells. Proc Natl Acad Sci U S A (2001) 1.39
Halophilic adaptation: novel solvent protein interactions observed in the 2.9 and 2.6 A resolution structures of the wild type and a mutant of malate dehydrogenase from Haloarcula marismortui. Biochemistry (2000) 1.26
Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. Proc Natl Acad Sci U S A (2001) 1.22
Crystal structure of the secretory form of membrane-associated human carbonic anhydrase IV at 2.8-A resolution. Proc Natl Acad Sci U S A (1996) 1.16
Inhibition and catalysis of carbonic anhydrase. Recent crystallographic analyses. Eur J Biochem (1994) 1.13
A salt-resistant plasma membrane carbonic anhydrase is induced by salt in Dunaliella salina. J Biol Chem (1996) 1.10
Superefficient enzymes. Cell Mol Life Sci (2001) 1.07
Crystal structure of halophilic dodecin: a novel, dodecameric flavin binding protein from Halobacterium salinarum. Structure (2003) 1.06
Expression, assay, and structure of the extracellular domain of murine carbonic anhydrase XIV: implications for selective inhibition of membrane-associated isozymes. J Biol Chem (2003) 1.04
Catalysis and inhibition of human carbonic anhydrase IV. Biochemistry (1997) 1.04
Contribution of long-range electrostatic interactions to the stabilization of the catalytic transition state of the serine protease subtilisin BPN'. Biochemistry (1993) 1.03
Monovalent cation-induced conformational change in glucose oxidase leading to stabilization of the enzyme. Biochemistry (2001) 0.95
Carbonic anhydrase XIV: luminal expression suggests key role in renal acidification. Kidney Int (2002) 0.95
The Oligomeric states of Haloarcula marismortui malate dehydrogenase are modulated by solvent components as shown by crystallographic and biochemical studies. J Mol Biol (2003) 0.93
The structure of human carbonic anhydrase II in complex with bromide and azide. FEBS Lett (1993) 0.93
Solvent interactions of halophilic malate dehydrogenase. Biochemistry (2002) 0.92
Insights into the molecular basis of salt tolerance from the study of glutamate dehydrogenase from Halobacterium salinarum. J Biol Chem (1998) 0.91
A Salt-Induced 60-Kilodalton Plasma Membrane Protein Plays a Potential Role in the Extreme Halotolerance of the Alga Dunaliella. Plant Physiol (1994) 0.90
Wild-type and E106Q mutant carbonic anhydrase complexed with acetate. Acta Crystallogr D Biol Crystallogr (1994) 0.88
Characterization of a salt-tolerant extracellular a-amylase from Bacillus dipsosauri. Lett Appl Microbiol (2002) 0.88
An unusual halotolerant alpha-type carbonic anhydrase from the alga Dunaliella salina functionally expressed in Escherichia coli. Protein Expr Purif (2003) 0.84
Natural protein engineering: a uniquely salt-tolerant, but not halophilic, alpha-type carbonic anhydrase from algae proliferating in low- to hyper-saline environments. Protein Eng Des Sel (2004) 0.82
Link between protein-solvent and weak protein-protein interactions gives insight into halophilic adaptation. Biochemistry (2002) 0.82
Molecular adaptation: the malate dehydrogenase from the extreme halophilic bacterium Salinibacter ruber behaves like a non-halophilic protein. Biochimie (2004) 0.81
Stability of Natrialba magadii NDP kinase: comparisons with other halophilic proteins. Extremophiles (2002) 0.80
Purification and characterisation of two extremely halotolerant xylanases from a novel halophilic bacterium. Extremophiles (2003) 0.79
Digestion by serine proteases enhances salt tolerance of glutaminase in the marine bacterium Micrococcus luteus K-3. Extremophiles (2004) 0.77
Identification, cDNA cloning, expression, crystallization and preliminary X-ray analysis of an exceptionally halotolerant carbonic anhydrase from Dunaliella salina. Acta Crystallogr D Biol Crystallogr (2003) 0.76
FoldIndex: a simple tool to predict whether a given protein sequence is intrinsically unfolded. Bioinformatics (2005) 5.44
Function and structure of inherently disordered proteins. Curr Opin Struct Biol (2008) 4.19
Protein production and purification. Nat Methods (2008) 3.97
Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Crystallogr D Biol Crystallogr (2005) 3.13
Proteopedia - a scientific 'wiki' bridging the rift between three-dimensional structure and function of biomacromolecules. Genome Biol (2008) 2.68
Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes. Nat Struct Mol Biol (2004) 2.46
Assessment of CASP8 structure predictions for template free targets. Proteins (2009) 1.96
Retrograde signaling by the plastidial metabolite MEcPP regulates expression of nuclear stress-response genes. Cell (2012) 1.87
Production of glucocerebrosidase with terminal mannose glycans for enzyme replacement therapy of Gaucher's disease using a plant cell system. Plant Biotechnol J (2007) 1.82
Acetylcholinesterase: 'classical' and 'non-classical' functions and pharmacology. Curr Opin Pharmacol (2005) 1.71
X-ray structure of human acid-beta-glucosidase, the defective enzyme in Gaucher disease. EMBO Rep (2003) 1.70
Evolved stereoselective hydrolases for broad-spectrum G-type nerve agent detoxification. Chem Biol (2012) 1.60
Directed evolution of hydrolases for prevention of G-type nerve agent intoxication. Nat Chem Biol (2011) 1.57
A server and database for dipole moments of proteins. Nucleic Acids Res (2007) 1.53
Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis. Nat Chem Biol (2012) 1.50
Proteomic signatures: amino acid and oligopeptide compositions differentiate among phyla. Proteins (2004) 1.45
Assessment of disorder predictions in CASP8. Proteins (2009) 1.44
Structural insights into substrate traffic and inhibition in acetylcholinesterase. EMBO J (2006) 1.41
Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies. Acta Crystallogr D Biol Crystallogr (2006) 1.39
Distinct roles of jasmonates and aldehydes in plant-defense responses. PLoS One (2008) 1.36
eMovie: a storyboard-based tool for making molecular movies. Trends Biochem Sci (2007) 1.29
The synaptic acetylcholinesterase tetramer assembles around a polyproline II helix. EMBO J (2004) 1.27
Acid beta-glucosidase: insights from structural analysis and relevance to Gaucher disease therapy. Biol Chem (2008) 1.25
Characterization of gene-activated human acid-beta-glucosidase: crystal structure, glycan composition, and internalization into macrophages. Glycobiology (2009) 1.25
Shoot-and-Trap: use of specific x-ray damage to study structural protein dynamics by temperature-controlled cryo-crystallography. Proc Natl Acad Sci U S A (2008) 1.24
Intronic T-DNA insertion renders Arabidopsis opr3 a conditional jasmonic acid-producing mutant. Plant Physiol (2011) 1.23
Acetylcholinesterase: from 3D structure to function. Chem Biol Interact (2010) 1.22
Operational definition of intrinsically unstructured protein sequences based on susceptibility to the 20S proteasome. Proteins (2008) 1.20
HalX: an open-source LIMS (Laboratory Information Management System) for small- to large-scale laboratories. Acta Crystallogr D Biol Crystallogr (2005) 1.19
X-ray structure of human acid-beta-glucosidase covalently bound to conduritol-B-epoxide. Implications for Gaucher disease. J Biol Chem (2005) 1.18
Crystal structure of thioflavin T bound to the peripheral site of Torpedo californica acetylcholinesterase reveals how thioflavin T acts as a sensitive fluorescent reporter of ligand binding to the acylation site. J Am Chem Soc (2008) 1.15
G protein-coupled receptors show unusual patterns of intrinsic unfolding. Protein Eng Des Sel (2005) 1.12
Evidence for the formation of disulfide radicals in protein crystals upon X-ray irradiation. J Synchrotron Radiat (2002) 1.12
Crystal structures of complexes of N-butyl- and N-nonyl-deoxynojirimycin bound to acid beta-glucosidase: insights into the mechanism of chemical chaperone action in Gaucher disease. J Biol Chem (2007) 1.11
Catalytic versatility and backups in enzyme active sites: the case of serum paraoxonase 1. J Mol Biol (2012) 1.08
New directions in the treatment of Gaucher disease. Trends Pharmacol Sci (2004) 1.07
Crystallographic snapshots of nonaged and aged conjugates of soman with acetylcholinesterase, and of a ternary complex of the aged conjugate with pralidoxime. J Med Chem (2009) 1.07
Complexes of alkylene-linked tacrine dimers with Torpedo californica acetylcholinesterase: Binding of Bis5-tacrine produces a dramatic rearrangement in the active-site gorge. J Med Chem (2006) 1.07
Induced-fit or preexisting equilibrium dynamics? Lessons from protein crystallography and MD simulations on acetylcholinesterase and implications for structure-based drug design. Protein Sci (2008) 1.05
Cis interaction between Semaphorin6A and Plexin-A4 modulates the repulsive response to Sema6A. EMBO J (2010) 1.05
Biophysical characterization of the unstructured cytoplasmic domain of the human neuronal adhesion protein neuroligin 3. Biophys J (2008) 1.03
Structural comparison of differently glycosylated forms of acid-beta-glucosidase, the defective enzyme in Gaucher disease. Acta Crystallogr D Biol Crystallogr (2006) 1.02
Proteopedia: a status report on the collaborative, 3D web-encyclopedia of proteins and other biomolecules. J Struct Biol (2011) 1.02
Genome-wide expression profiling Arabidopsis at the stage of Golovinomyces cichoracearum haustorium formation. Plant Physiol (2008) 1.01
Genome-wide comparative analysis of the IQD gene families in Arabidopsis thaliana and Oryza sativa. BMC Evol Biol (2005) 1.00
Acetylcholinesterase: how is structure related to function? Chem Biol Interact (2008) 1.00
Flexibility of metal binding sites in proteins on a database scale. Proteins (2005) 0.99
Use of a 'caged' analogue to study the traffic of choline within acetylcholinesterase by kinetic crystallography. Acta Crystallogr D Biol Crystallogr (2007) 0.99
The intracellular domain of the Drosophila cholinesterase-like neural adhesion protein, gliotactin, is natively unfolded. Proteins (2003) 0.98
Acetylcholinesterase: a multifaceted target for structure-based drug design of anticholinesterase agents for the treatment of Alzheimer's disease. J Mol Neurosci (2003) 0.97
How does huperzine A enter and leave the binding gorge of acetylcholinesterase? Steered molecular dynamics simulations. J Am Chem Soc (2003) 0.96
Flexibility of aromatic residues in the active-site gorge of acetylcholinesterase: X-ray versus molecular dynamics. Biophys J (2008) 0.95
The complex of a bivalent derivative of galanthamine with torpedo acetylcholinesterase displays drastic deformation of the active-site gorge: implications for structure-based drug design. J Am Chem Soc (2004) 0.94
6-Amino-6-deoxy-5,6-di-N-(N'-octyliminomethylidene)nojirimycin: synthesis, biological evaluation, and crystal structure in complex with acid beta-glucosidase. Chembiochem (2009) 0.92
Kinetics of precursor interactions with the bacterial Tat translocase detected by real-time FRET. J Biol Chem (2012) 0.92
Acetylcholinesterase complexed with bivalent ligands related to huperzine a: experimental evidence for species-dependent protein-ligand complementarity. J Am Chem Soc (2003) 0.91
Dynamic mechanism of E2020 binding to acetylcholinesterase: a steered molecular dynamics simulation. J Phys Chem B (2005) 0.91
Catalytic metal ion rearrangements underline promiscuity and evolvability of a metalloenzyme. J Mol Biol (2013) 0.91
Crystal packing mediates enantioselective ligand recognition at the peripheral site of acetylcholinesterase. J Am Chem Soc (2005) 0.90
Refined structure of bovine carboxypeptidase A at 1.25 A resolution. Acta Crystallogr D Biol Crystallogr (2003) 0.90
Flexibility of the flap in the active site of BACE1 as revealed by crystal structures and molecular dynamics simulations. Acta Crystallogr D Biol Crystallogr (2011) 0.89
Origin of β-carotene-rich plastoglobuli in Dunaliella bardawil. Plant Physiol (2014) 0.88
The binding site of acetylcholine receptor: from synthetic peptides to solution and crystal structure. Ann N Y Acad Sci (2003) 0.88
Bacterial Sec protein transport is rate-limited by precursor length: a single turnover study. Mol Biol Cell (2009) 0.88
Long route or shortcut? A molecular dynamics study of traffic of thiocholine within the active-site gorge of acetylcholinesterase. Biophys J (2010) 0.88
Expression of protein complexes using multiple Escherichia coli protein co-expression systems: a benchmarking study. J Struct Biol (2011) 0.87
The 3D structure of the anticancer prodrug CPT-11 with Torpedo californica acetylcholinesterase rationalizes its inhibitory action on AChE and its hydrolysis by butyrylcholinesterase and carboxylesterase. Chem Biol Interact (2005) 0.87
Proteopedia: A collaborative, virtual 3D web-resource for protein and biomolecule structure and function. Biochem Mol Biol Educ (2010) 0.86
Structure of estradiol metal chelate and estrogen receptor complex: the basis for designing a new class of selective estrogen receptor modulators. J Med Chem (2011) 0.86
Analysis of genetic polymorphisms in acetylcholinesterase as reflected in different populations. Curr Alzheimer Res (2005) 0.86
RIKEN aids international structural genomics efforts. Nature (2007) 0.86
Design and synthesis of peptides that bind alpha-bungarotoxin with high affinity and mimic the three-dimensional structure of the binding-site of acetylcholine receptor. Biophys Chem (2003) 0.85
Backdoor opening mechanism in acetylcholinesterase based on X-ray crystallography and molecular dynamics simulations. Protein Sci (2011) 0.85
3-D structure of serum paraoxonase 1 sheds light on its activity, stability, solubility and crystallizability. Arh Hig Rada Toksikol (2007) 0.85
Structure of a complex of the potent and specific inhibitor BW284C51 with Torpedo californica acetylcholinesterase. Acta Crystallogr D Biol Crystallogr (2002) 0.85
Salt induction of fatty acid elongase and membrane lipid modifications in the extreme halotolerant alga Dunaliella salina. Plant Physiol (2002) 0.84
Molecular basis of reduced glucosylceramidase activity in the most common Gaucher disease mutant, N370S. J Biol Chem (2010) 0.84
Isolation and characterization of a xanthophyll-rich fraction from the thylakoid membrane of Dunaliella salina(green algae). Photochem Photobiol Sci (2005) 0.84
Structural proteomics: from the molecule to the system. Nat Struct Mol Biol (2007) 0.84
An unusual halotolerant alpha-type carbonic anhydrase from the alga Dunaliella salina functionally expressed in Escherichia coli. Protein Expr Purif (2003) 0.84
Context-dependent resistance to proteolysis of intrinsically disordered proteins. Protein Sci (2011) 0.84
Directed evolution of serum paraoxonase PON3 by family shuffling and ancestor/consensus mutagenesis, and its biochemical characterization. Biochemistry (2009) 0.83
Criteria for selecting PEGylation sites on proteins for higher thermodynamic and proteolytic stability. J Am Chem Soc (2014) 0.83
Arabidopsis calmodulin-binding protein IQ67-domain 1 localizes to microtubules and interacts with kinesin light chain-related protein-1. J Biol Chem (2012) 0.83
The 0.93A crystal structure of sphericase: a calcium-loaded serine protease from Bacillus sphaericus. J Mol Biol (2003) 0.83
Control of the rate of evaporation in protein crystallization by the 'microbatch under oil' method. J Appl Crystallogr (2008) 0.82
A neutral molecule in a cation-binding site: specific binding of a PEG-SH to acetylcholinesterase from Torpedo californica. J Mol Biol (2002) 0.82