Published in Nat Methods on December 01, 2004
The rough energy landscape of superfolder GFP is linked to the chromophore. J Mol Biol (2007) 1.23
An ER-peroxisome tether exerts peroxisome population control in yeast. EMBO J (2013) 1.15
Fluorescence applications in molecular neurobiology. Neuron (2010) 1.13
Formation and toxicity of soluble polyglutamine oligomers in living cells. PLoS One (2010) 1.12
The catalytic activity of protein-disulfide isomerase requires a conformationally flexible molecule. J Biol Chem (2008) 1.12
Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells. Mol Cell Proteomics (2010) 1.12
A dual function for Pex3p in peroxisome formation and inheritance. J Cell Biol (2009) 1.11
Bimolecular fluorescence complementation (BiFC) analysis of protein interactions in Caenorhabditis elegans. Methods (2008) 1.06
Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells. J Neurosci Methods (2011) 1.03
Histidine-containing phosphotransfer protein-B (HptB) regulates swarming motility through partner-switching system in Pseudomonas aeruginosa PAO1 strain. J Biol Chem (2011) 1.00
Visualizing interactions along the Escherichia coli twin-arginine translocation pathway using protein fragment complementation. PLoS One (2010) 0.99
Metallo-GTPase HypB from Helicobacter pylori and its interaction with nickel chaperone protein HypA. J Biol Chem (2011) 0.95
Intermolecular interactions between retroviral Gag proteins in the nucleus. J Virol (2007) 0.92
Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). J Bacteriol (2010) 0.90
Interactions between the influenza A virus RNA polymerase components and retinoic acid-inducible gene I. J Virol (2014) 0.90
Designed proteins to modulate cellular networks. ACS Chem Biol (2010) 0.89
Akt regulates TPP1 homodimerization and telomere protection. Aging Cell (2013) 0.88
Hysteresis as a Marker for Complex, Overlapping Landscapes in Proteins. J Phys Chem Lett (2012) 0.88
Resurfaced shape complementary proteins that selectively bind the oncoprotein gankyrin. ACS Chem Biol (2014) 0.86
Modular extracellular sensor architecture for engineering mammalian cell-based devices. ACS Synth Biol (2014) 0.86
Balboa binds to pickpocket in vivo and is required for mechanical nociception in Drosophila larvae. Curr Biol (2014) 0.84
Green fluorescence induced by EF-hand assembly in a split GFP system. Protein Sci (2009) 0.83
Fluorescence complementation via EF-hand interactions. J Biotechnol (2009) 0.82
Cre reconstitution allows for DNA recombination selectively in dual-marker-expressing cells in transgenic mice. Nucleic Acids Res (2007) 0.82
Protein fragment bimolecular fluorescence complementation analyses for the in vivo study of protein-protein interactions and cellular protein complex localizations. Methods Mol Biol (2014) 0.82
Red fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications. Neurophotonics (2015) 0.81
Fluorescent labeling of antibody fragments using split GFP. PLoS One (2011) 0.81
A split luciferase complementation assay for studying in vivo protein-protein interactions in filamentous ascomycetes. Curr Genet (2012) 0.80
Comprehensive analysis of LANA interacting proteins essential for viral genome tethering and persistence. PLoS One (2013) 0.80
In vivo protein stabilization based on fragment complementation and a split GFP system. Proc Natl Acad Sci U S A (2010) 0.79
Combining random gene fission and rational gene fusion to discover near-infrared fluorescent protein fragments that report on protein-protein interactions. ACS Synth Biol (2014) 0.79
In silico protein fragmentation reveals the importance of critical nuclei on domain reassembly. Biophys J (2007) 0.78
Plasmodium falciparum Hop (PfHop) Interacts with the Hsp70 Chaperone in a Nucleotide-Dependent Fashion and Exhibits Ligand Selectivity. PLoS One (2015) 0.78
Pseudomonas aeruginosa PA1006 is a persulfide-modified protein that is critical for molybdenum homeostasis. PLoS One (2013) 0.78
One-step generation of error-prone PCR libraries using Gateway® technology. Microb Cell Fact (2012) 0.77
Structural Disorder within Paramyxoviral Nucleoproteins and Phosphoproteins in Their Free and Bound Forms: From Predictions to Experimental Assessment. Int J Mol Sci (2015) 0.77
RhoGDI3 and RhoG: Vesicular trafficking and interactions with the Sec3 Exocyst subunit. Small GTPases (2010) 0.76
Heterologous protein-DNA interactions lead to biased allelic expression of circadian clock genes in interspecific hybrids. Sci Rep (2017) 0.75
Kinetics and reaction coordinates of the reassembly of protein fragments via forward flux sampling. Biophys J (2010) 0.75
Characterization of the Interaction Between the Small Regulatory Peptide SgrT and the EIICBGlc of the Glucose-Phosphotransferase System of E. coli K-12. Metabolites (2012) 0.75
Specific cell surface labeling of GPCRs using split GFP. Sci Rep (2016) 0.75
Detecting soluble polyQ oligomers and investigating their impact on living cells using split-GFP. Methods Mol Biol (2013) 0.75
New cell line development for antibody-producing Chinese hamster ovary cells using split green fluorescent protein. BMC Biotechnol (2012) 0.75
Attenuating Staphylococcus aureus Virulence by Targeting Flotillin Protein Scaffold Activity. Cell Chem Biol (2017) 0.75
TPR proteins: the versatile helix. Trends Biochem Sci (2003) 7.37
Design of stable alpha-helical arrays from an idealized TPR motif. Structure (2003) 3.17
Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism. J Am Chem Soc (2005) 2.95
Structure and function of KH domains. FEBS J (2008) 2.48
Exploring the limits of codon and anticodon size. Chem Biol (2002) 1.89
High-throughput thermal scanning: a general, rapid dye-binding thermal shift screen for protein engineering. J Am Chem Soc (2009) 1.85
A recurring theme in protein engineering: the design, stability and folding of repeat proteins. Curr Opin Struct Biol (2005) 1.58
A new folding paradigm for repeat proteins. J Am Chem Soc (2005) 1.57
The interface of protein structure, protein biophysics, and molecular evolution. Protein Sci (2012) 1.54
The folding and design of repeat proteins: reaching a consensus. Curr Opin Struct Biol (2003) 1.43
Sequence variation in ligand binding sites in proteins. BMC Bioinformatics (2005) 1.37
Structure and stability of designed TPR protein superhelices: unusual crystal packing and implications for natural TPR proteins. Acta Crystallogr D Biol Crystallogr (2007) 1.33
A direct interaction between the Utp6 half-a-tetratricopeptide repeat domain and a specific peptide in Utp21 is essential for efficient pre-rRNA processing. Mol Cell Biol (2008) 1.30
Protein design to understand peptide ligand recognition by tetratricopeptide repeat proteins. Protein Eng Des Sel (2004) 1.28
Designed TPR modules as novel anticancer agents. ACS Chem Biol (2008) 1.28
Local and long-range stability in tandemly arrayed tetratricopeptide repeats. Proc Natl Acad Sci U S A (2005) 1.28
Ligand binding by TPR domains. Protein Sci (2006) 1.25
Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation. Proc Natl Acad Sci U S A (2013) 1.21
Protein-protein interactions: general trends in the relationship between binding affinity and interfacial buried surface area. Protein Sci (2013) 1.20
Ligand binding by repeat proteins: natural and designed. Curr Opin Struct Biol (2008) 1.19
A novel class of small molecule inhibitors of Hsp90. ACS Chem Biol (2008) 1.19
Fragile X mental retardation syndrome: structure of the KH1-KH2 domains of fragile X mental retardation protein. Structure (2007) 1.18
Balance between folding and degradation for Hsp90-dependent client proteins: a key role for CHIP. Biochemistry (2010) 1.17
Beyond consensus: statistical free energies reveal hidden interactions in the design of a TPR motif. J Mol Biol (2004) 1.14
Identification of residues on Hsp70 and Hsp90 ubiquitinated by the cochaperone CHIP. J Mol Biol (2009) 1.11
Direct single-molecule observation of a protein living in two opposed native structures. Proc Natl Acad Sci U S A (2009) 1.07
A new bright green-emitting fluorescent protein--engineered monomeric and dimeric forms. FEBS J (2010) 1.04
Stabilizing proteins from sequence statistics: the interplay of conservation and correlation in triosephosphate isomerase stability. J Mol Biol (2012) 1.03
Triosephosphate isomerase by consensus design: dramatic differences in physical properties and activity of related variants. J Mol Biol (2011) 1.01
Consensus design as a tool for engineering repeat proteins. Methods Mol Biol (2006) 1.01
Revisiting the Ramachandran plot from a new angle. Protein Sci (2011) 1.00
Combinatorial approaches to protein stability and structure. Eur J Biochem (2004) 0.99
Covariation of backbone motion throughout a small protein domain. Nat Struct Biol (2003) 0.98
The crystal structure of NlpI. A prokaryotic tetratricopeptide repeat protein with a globular fold. FEBS J (2005) 0.97
Crystal structure of a designed tetratricopeptide repeat module in complex with its peptide ligand. FEBS J (2010) 0.96
Redesign of a protein-peptide interaction: characterization and applications. Protein Sci (2009) 0.96
Electrostatic interactions of Hsp-organizing protein tetratricopeptide domains with Hsp70 and Hsp90: computational analysis and protein engineering. J Biol Chem (2009) 0.95
A compound that inhibits the HOP-Hsp90 complex formation and has unique killing effects in breast cancer cell lines. Mol Pharm (2011) 0.95
Mutations in the B1 domain of protein G that delay the onset of amyloid fibril formation in vitro. Protein Sci (2003) 0.94
Mapping the energy landscape of repeat proteins using NMR-detected hydrogen exchange. J Mol Biol (2008) 0.94
Screening libraries to identify proteins with desired binding activities using a split-GFP reassembly assay. ACS Chem Biol (2010) 0.93
Cysteine-free Rop: a four-helix bundle core mutant has wild-type stability and structure but dramatically different unfolding kinetics. Protein Sci (2010) 0.92
Non-random-coil behavior as a consequence of extensive PPII structure in the denatured state. J Mol Biol (2008) 0.92
A cell-based screen for function of the four-helix bundle protein Rop: a new tool for combinatorial experiments in biophysics. Protein Eng Des Sel (2004) 0.92
HOP is a monomer: investigation of the oligomeric state of the co-chaperone HOP. Protein Sci (2010) 0.92
Mutational analysis of 48G7 reveals that somatic hypermutation affects both antibody stability and binding affinity. J Am Chem Soc (2013) 0.91
Calorimetric study of a series of designed repeat proteins: modular structure and modular folding. Protein Sci (2011) 0.90
Kinetics and thermodynamics of phenotype: unwinding and rewinding the nucleosome. J Mol Biol (2012) 0.90
An AlphaScreen-based high-throughput screen to identify inhibitors of Hsp90-cochaperone interaction. J Biomol Screen (2009) 0.90
Stimuli-responsive smart gels realized via modular protein design. J Am Chem Soc (2010) 0.89
A new method for the covalent attachment of DNA to a surface for single-molecule studies. Colloids Surf B Biointerfaces (2010) 0.89
Dramatic differences in organophosphorus hydrolase activity between human and chimeric recombinant mammalian paraoxonase-1 enzymes. Biochemistry (2009) 0.89
Designed proteins to modulate cellular networks. ACS Chem Biol (2010) 0.89
A twisted four-sheeted model for an amyloid fibril. Structure (2005) 0.88
Creating novel proteins by combining design and selection. Protein Eng Des Sel (2010) 0.87
Analysis of side chain mobility among protein G B1 domain mutants with widely varying stabilities. Protein Sci (2004) 0.85
Reassembled GFP: detecting protein-protein interactions and protein expression patterns. Methods Biochem Anal (2006) 0.85
A modular approach to the design of protein-based smart gels. Biopolymers (2012) 0.85
Repeat motions and backbone flexibility in designed proteins with different numbers of identical consensus tetratricopeptide repeats. Biochemistry (2006) 0.84
Re-engineering a split-GFP reassembly screen to examine RING-domain interactions between BARD1 and BRCA1 mutants observed in cancer patients. Mol Biosyst (2008) 0.84
An antibody with a variable-region coiled-coil "knob" domain. Angew Chem Int Ed Engl (2013) 0.84
A structural model for the HAT domain of Utp6 incorporating bioinformatics and genetics. Protein Eng Des Sel (2009) 0.84
Does the location of a mutation determine the ability to form amyloid fibrils? J Mol Biol (2002) 0.83
The power of hard-sphere models: explaining side-chain dihedral angle distributions of Thr and Val. Biophys J (2012) 0.82
New insights into Fragile X syndrome. Relating genotype to phenotype at the molecular level. FEBS J (2005) 0.82
Characterization of folding the four-helix bundle protein Rop by real-time NMR. Protein Eng Des Sel (2008) 0.82
Designed phosphoprotein recognition in Escherichia coli. ACS Chem Biol (2014) 0.81
Protein engineering and stabilization from sequence statistics: variation and covariation analysis. Methods Enzymol (2013) 0.81
Predicting the side-chain dihedral angle distributions of nonpolar, aromatic, and polar amino acids using hard sphere models. Proteins (2014) 0.81
Simplifying protein expression with ligation-free, traceless and tag-switching plasmids. Protein Expr Purif (2012) 0.80
Addressing the unmet need for visualizing conditional random fields in biological data. BMC Bioinformatics (2014) 0.80
Conservation of mechanism, variation of rate: folding kinetics of three homologous four-helix bundle proteins. Protein Eng Des Sel (2008) 0.79
Modulating repeat protein stability: the effect of individual helix stability on the collective behavior of the ensemble. Protein Sci (2011) 0.79
Expanding the natural repertoire of protein structure and function. Curr Pharm Biotechnol (2002) 0.78
Nanostructured functional films from engineered repeat proteins. J R Soc Interface (2013) 0.78
New materials from proteins and peptides. Curr Opin Struct Biol (2012) 0.78
Solubilization and humanization of paraoxonase-1. J Lipids (2012) 0.78
New insights into the interdependence between amino acid stereochemistry and protein structure. Biophys J (2013) 0.77
Expression and purification of a recombinant amyloidogenic peptide from transthyretin for solid-state NMR spectroscopy. Protein Expr Purif (2009) 0.77
Mechanistic Insights into the Hydrolysis of Organophosphorus Compounds by Paraoxonase-1: Exploring the Limits of Substrate Tolerance in a Promiscuous Enzyme. J Phys Org Chem (2012) 0.77
Comparison of the backbone dynamics of a natural and a consensus designed 3-TPR domain. J Biomol NMR (2008) 0.76
Probing the Molecular Origin of Native-State Flexibility in Repeat Proteins. J Am Chem Soc (2015) 0.76
Synthetic approach to stop-codon scanning mutagenesis. J Am Chem Soc (2011) 0.75
Site-specific protein double labeling by expressed protein ligation: applications to repeat proteins. Org Biomol Chem (2011) 0.75
Intrinsic α-helical and β-sheet conformational preferences: a computational case study of alanine. Protein Sci (2014) 0.75
White and green screening with circular polymerase extension cloning for easy and reliable cloning. Protein Sci (2013) 0.75
Protein engineering and design: from first principles to new technologies. Curr Opin Struct Biol (2010) 0.75
Editorial overview: Mechanistic biology: Dynamic interactions in biology-sensing change. Curr Opin Chem Biol (2015) 0.75
Flat Drops, Elastic Sheets and Microcapsules by Interfacial Assembly of a Bacterial Biofilm Protein, BslA. Langmuir (2017) 0.75
Library approaches to biophysical problems. Eur J Biochem (2004) 0.75
Protein folding and design: from simple models to complex systems. Curr Opin Struct Biol (2008) 0.75