Published in Biochem Biophys Res Commun on January 23, 2007
Directed evolution of an orthogonal nucleoside analog kinase via fluorescence-activated cell sorting. Nucleic Acids Res (2009) 0.97
Non-homologous recombination of deoxyribonucleoside kinases from human and Drosophila melanogaster yields human-like enzymes with novel activities. J Mol Biol (2007) 0.83
Redesigning human 2'-deoxycytidine kinase enantioselectivity for L-nucleoside analogues as reporters in positron emission tomography. ACS Chem Biol (2014) 0.77
The phylogenetic distribution and evolution of enzymes within the thymidine kinase 2-like gene family in metazoa. J Mol Evol (2014) 0.77
Engineering Kinases to Phosphorylate Nucleoside Analogs for Antiviral and Cancer Therapy. Chimia (Aarau) (2009) 0.75
Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem (1989) 29.15
SWISS-MODEL: An automated protein homology-modeling server. Nucleic Acids Res (2003) 25.86
3DCoffee: combining protein sequences and structures within multiple sequence alignments. J Mol Biol (2004) 6.01
Mammalian deoxyribonucleoside kinases. Pharmacol Ther (1995) 3.31
Mutant mitochondrial thymidine kinase in mitochondrial DNA depletion myopathy. Nat Genet (2001) 2.91
Structure of human dCK suggests strategies to improve anticancer and antiviral therapy. Nat Struct Biol (2003) 1.83
Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing Heat-shock proteins. J Biol Chem (1996) 1.45
Human thymidine kinase 2: molecular cloning and characterisation of the enzyme activity with antiviral and cytostatic nucleoside substrates. FEBS Lett (1999) 1.34
Substrate specificity and phosphorylation of antiviral and anticancer nucleoside analogues by human deoxyribonucleoside kinases and ribonucleoside kinases. Pharmacol Ther (2003) 1.18
A second-generation system for unbiased reading frame selection. Protein Eng Des Sel (2004) 1.14
Selective assays for thymidine kinase 1 and 2 and deoxycytidine kinase and their activities in extracts from human cells and tissues. Biochem Biophys Res Commun (1992) 1.12
Functional expression of a multisubstrate deoxyribonucleoside kinase from Drosophila melanogaster and its C-terminal deletion mutants. J Biol Chem (2000) 1.11
Cloning of the cDNA and chromosome localization of the gene for human thymidine kinase 2. J Biol Chem (1997) 1.11
Structural basis for substrate specificities of cellular deoxyribonucleoside kinases. Nat Struct Biol (2001) 1.07
Divergent effects of chaperone overexpression and ethanol supplementation on inclusion body formation in recombinant Escherichia coli. Protein Expr Purif (1997) 1.03
A few amino acid substitutions can convert deoxyribonucleoside kinase specificity from pyrimidines to purines. EMBO J (2002) 0.98
Uridine-cytidine kinase from Escherichia coli. Methods Enzymol (1978) 0.97
Tight binding of deoxyribonucleotide triphosphates to human thymidine kinase 2 expressed in Escherichia coli. Purification and partial characterization of its dimeric and tetrameric forms. Biochemistry (2003) 0.96
Deoxyribonucleoside kinases: two enzyme families catalyze the same reaction. Trends Biochem Sci (2005) 0.93
Structural basis for feedback inhibition of the deoxyribonucleoside salvage pathway: studies of the Drosophila deoxyribonucleoside kinase. Biochemistry (2003) 0.92
Effect of ethanol and low-temperature culture on expression of soybean lipoxygenase L-1 in Escherichia coli. Protein Expr Purif (1992) 0.92
Deoxyribonucleoside kinases belonging to the thymidine kinase 2 (TK2)-like group vary significantly in substrate specificity, kinetics and feed-back regulation. J Mol Biol (2002) 0.86
Kinetic analysis and ligand-induced conformational changes in dimeric and tetrameric forms of human thymidine kinase 2. Biochemistry (2005) 0.86
Mosquito has a single multisubstrate deoxyribonucleoside kinase characterized by unique substrate specificity. Nucleic Acids Res (2003) 0.84
Coupling interactions of distal residues enhance dihydrofolate reductase catalysis: mutational effects on hydride transfer rates. Biochemistry (2002) 2.09
PCR amplification of DNA containing non-standard base pairs by variants of reverse transcriptase from Human Immunodeficiency Virus-1. Nucleic Acids Res (2004) 1.52
Improving the catalytic activity of Candida antarctica lipase B by circular permutation. J Am Chem Soc (2005) 1.24
Circular permutation: a different way to engineer enzyme structure and function. Trends Biotechnol (2010) 1.16
A second-generation system for unbiased reading frame selection. Protein Eng Des Sel (2004) 1.14
Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure. J Mol Biol (2007) 1.12
Systematic exploration of active site mutations on human deoxycytidine kinase substrate specificity. Biochemistry (2008) 1.03
Directed evolution of an orthogonal nucleoside analog kinase via fluorescence-activated cell sorting. Nucleic Acids Res (2009) 0.97
Quaternary structure change as a mechanism for the regulation of thymidine kinase 1-like enzymes. Structure (2007) 0.95
Circular permutation of Bacillus circulans xylanase: a kinetic and structural study. Biochemistry (2010) 0.95
Structural redesign of lipase B from Candida antarctica by circular permutation and incremental truncation. J Mol Biol (2009) 0.88
Investigating the structural and functional consequences of circular permutation on lipase B from Candida antarctica. Chembiochem (2007) 0.88
Engineering enzymes by 'intelligent' design. Curr Opin Chem Biol (2009) 0.87
Improved biocatalysts from a synthetic circular permutation library of the flavin-dependent oxidoreductase old yellow enzyme. J Am Chem Soc (2013) 0.86
The creation of ITCHY hybrid protein libraries. Methods Mol Biol (2003) 0.85
Recent progress in engineering alpha/beta hydrolase-fold family members. Biotechnol J (2007) 0.84
Non-homologous recombination of deoxyribonucleoside kinases from human and Drosophila melanogaster yields human-like enzymes with novel activities. J Mol Biol (2007) 0.83
Improved triglyceride transesterification by circular permuted Candida antarctica lipase B. Biotechnol Bioeng (2010) 0.81
1,5-Alpha-D-mannoseptanosides, ring-size isomers that are impervious to alpha-mannosidase-catalyzed hydrolysis. Org Lett (2009) 0.80
Computational design of orthogonal nucleoside kinases. Chem Commun (Camb) (2010) 0.80
On the structural and functional modularity of glycinamide ribonucleotide formyltransferases. Protein Sci (2003) 0.79
Just (protein) engineering? Curr Opin Struct Biol (2013) 0.77
Redesigning human 2'-deoxycytidine kinase enantioselectivity for L-nucleoside analogues as reporters in positron emission tomography. ACS Chem Biol (2014) 0.77
Synthesis of fluorescent nucleoside analogs as probes for 2'-deoxyribonucleoside kinases. Bioorg Med Chem Lett (2010) 0.76
Engineering Kinases to Phosphorylate Nucleoside Analogs for Antiviral and Cancer Therapy. Chimia (Aarau) (2009) 0.75
[Renal failure and recurrent episodes of flash pulmonary edema in a 70-year-old patient]. Dtsch Med Wochenschr (2013) 0.75
Novel protease inhibitors via computational redesign of subtilisin BPN' propeptide. Biochemistry (2012) 0.75
Truncated FAD synthetase for direct biocatalytic conversion of riboflavin and analogs to their corresponding flavin mononucleotides. Protein Eng Des Sel (2013) 0.75