Genetic incorporation of unnatural amino acids into proteins in mammalian cells.

Bioorthogonal chemistry: fishing for selectivity in a sea of functionality. Angew Chem Int Ed Engl (2009) 8.15

A fluorophore ligase for site-specific protein labeling inside living cells. Proc Natl Acad Sci U S A (2010) 2.91

Fluorescent analogs of biomolecular building blocks: design, properties, and applications. Chem Rev (2010) 2.16

A facile system for encoding unnatural amino acids in mammalian cells. Angew Chem Int Ed Engl (2009) 2.02

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids. Proc Natl Acad Sci U S A (2012) 1.99

Expanding the genetic code for biological studies. Chem Biol (2009) 1.85

Site-specific antibody drug conjugates for cancer therapy. MAbs (2014) 1.74

Site-directed spin labeling of a genetically encoded unnatural amino acid. Proc Natl Acad Sci U S A (2009) 1.65

An engineered aryl azide ligase for site-specific mapping of protein-protein interactions through photo-cross-linking. Angew Chem Int Ed Engl (2008) 1.61

In vivo incorporation of unnatural amino acids to probe structure, dynamics, and ligand binding in a large protein by nuclear magnetic resonance spectroscopy. J Am Chem Soc (2008) 1.57

A genetically encoded boronate-containing amino acid. Angew Chem Int Ed Engl (2008) 1.54

Beyond the canonical 20 amino acids: expanding the genetic lexicon. J Biol Chem (2010) 1.53

FTIR analysis of GPCR activation using azido probes. Nat Chem Biol (2009) 1.52

Ligand-directed tosyl chemistry for protein labeling in vivo. Nat Chem Biol (2009) 1.43

Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae. J Am Chem Soc (2009) 1.42

A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum. J Am Chem Soc (2012) 1.42

Quality control despite mistranslation caused by an ambiguous genetic code. Proc Natl Acad Sci U S A (2008) 1.36

Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids. ACS Chem Biol (2011) 1.28

A versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry (2013) 1.28

Protein nuclear magnetic resonance under physiological conditions. Biochemistry (2009) 1.23

An engineered selenocysteine defines a unique class of antibody derivatives. Proc Natl Acad Sci U S A (2008) 1.21

Peptide-directed self-assembly of hydrogels. Acta Biomater (2008) 1.15

Functional analysis of human tRNA isodecoders. J Mol Biol (2009) 1.13

Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool. Biochim Biophys Acta (2014) 1.13

A genetically encoded acrylamide functionality. ACS Chem Biol (2013) 1.12

Protein-based block copolymers. Biomacromolecules (2011) 1.11

Monitoring protein interactions and dynamics with solvatochromic fluorophores. Trends Biotechnol (2009) 1.10

Synthetic constructs in/for the environment: managing the interplay between natural and engineered Biology. FEBS Lett (2012) 1.09

Genetic incorporation of twelve meta-substituted phenylalanine derivatives using a single pyrrolysyl-tRNA synthetase mutant. ACS Chem Biol (2012) 1.06

Antibody-drug conjugates as novel anti-cancer chemotherapeutics. Biosci Rep (2015) 1.03

Expanding the genetic code of Caenorhabditis elegans using bacterial aminoacyl-tRNA synthetase/tRNA pairs. ACS Chem Biol (2012) 1.01

Xenobiology: a new form of life as the ultimate biosafety tool. Bioessays (2010) 1.00

Site-specific labeling of proteins with NMR-active unnatural amino acids. J Biomol NMR (2009) 1.00

Genetically encoding unnatural amino acids in neural stem cells and optically reporting voltage-sensitive domain changes in differentiated neurons. Stem Cells (2011) 0.99

Genetic incorporation of unnatural amino acids into proteins in Mycobacterium tuberculosis. PLoS One (2010) 0.97

Site-selective protein-modification chemistry for basic biology and drug development. Nat Chem (2015) 0.96

Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc Natl Acad Sci U S A (2013) 0.96

Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code. PLoS One (2012) 0.95

Uniform fluorescent nanobioprobes for pathogen detection. ACS Nano (2014) 0.94

Site-specific protein modifications through pyrroline-carboxy-lysine residues. Proc Natl Acad Sci U S A (2011) 0.94

Site-specific orthogonal labeling of the carboxy terminus of alpha-tubulin. ACS Chem Biol (2010) 0.93

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods (2016) 0.93

Genetic encoding of non-natural amino acids in Drosophila melanogaster Schneider 2 cells. Protein Sci (2010) 0.92

Functional replacement of the endogenous tyrosyl-tRNA synthetase-tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion. Nucleic Acids Res (2010) 0.92

Expanding the substrate tolerance of biotin ligase through exploration of enzymes from diverse species. J Am Chem Soc (2008) 0.91

Towards reassigning the rare AGG codon in Escherichia coli. Chembiochem (2014) 0.89

Genetic incorporation of seven ortho-substituted phenylalanine derivatives. ACS Chem Biol (2014) 0.89

Investigation of an unnatural amino acid for use as a resonance Raman probe: Detection limits, solvent and temperature dependence of the νC≡N band of 4-cyanophenylalanine. J Raman Spectrosc (2008) 0.89

Synthetase polyspecificity as a tool to modulate protein function. Bioorg Med Chem Lett (2011) 0.88

Synthetic biology: putting synthesis into biology. Wiley Interdiscip Rev Syst Biol Med (2010) 0.87

Site-specific antibody-polymer conjugates for siRNA delivery. J Am Chem Soc (2013) 0.87

Synthetic biology approaches in drug discovery and pharmaceutical biotechnology. Appl Microbiol Biotechnol (2010) 0.87

Strategies and Advancement in Antibody-Drug Conjugate Optimization for Targeted Cancer Therapeutics. Biomol Ther (Seoul) (2015) 0.86

Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells. PLoS One (2010) 0.85

Current Status: Site-Specific Antibody Drug Conjugates. J Clin Immunol (2016) 0.84

SH3 domain-based phototrapping in living cells reveals Rho family GAP signaling complexes. Sci Signal (2011) 0.84

The charge-dipole pocket: a defining feature of signaling pathway GTPase on/off switches. J Mol Biol (2009) 0.84

N6-(2-(R)-propargylglycyl)lysine as a clickable pyrrolysine mimic. Chem Asian J (2010) 0.83

Response and adaptation of Escherichia coli to suppression of the amber stop codon. Chembiochem (2014) 0.82

The application of modular protein domains in proteomics. FEBS Lett (2012) 0.82

Rational design and directed evolution of a bacterial-type glutaminyl-tRNA synthetase precursor. Nucleic Acids Res (2012) 0.82

In vitro incorporation of nonnatural amino acids into protein using tRNA(Cys)-derived opal, ochre, and amber suppressor tRNAs. RNA (2010) 0.81

Importance of single molecular determinants in the fidelity of expanded genetic codes. Proc Natl Acad Sci U S A (2011) 0.81

Multiformat T-cell-engaging bispecific antibodies targeting human breast cancers. Angew Chem Int Ed Engl (2015) 0.81

Esterification of an unnatural amino acid structurally deviating from canonical amino acids promotes its uptake and incorporation into proteins in mammalian cells. Chembiochem (2010) 0.80

An efficient protocol for incorporation of an unnatural amino acid in perdeuterated recombinant proteins using glucose-based media. J Biomol NMR (2012) 0.80

Genetic incorporation of histidine derivatives using an engineered pyrrolysyl-tRNA synthetase. ACS Chem Biol (2014) 0.80

Chemical tools for K(+) channel biology. Biochemistry (2009) 0.80

Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli. ACS Synth Biol (2015) 0.80

Construction of a live-attenuated HIV-1 vaccine through genetic code expansion. Angew Chem Int Ed Engl (2014) 0.80

Peptide array X-linking (PAX): a new peptide-protein identification approach. PLoS One (2012) 0.78

Micelle-Enhanced Bioorthogonal Labeling of Genetically Encoded Azido Groups on the Lipid-Embedded Surface of a GPCR. Chembiochem (2015) 0.78

Specific modulation of protein activity by using a bioorthogonal reaction. Chembiochem (2014) 0.77

Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers. ACS Chem Biol (2014) 0.77

Genetic Incorporation of the Unnatural Amino Acid p-Acetyl Phenylalanine into Proteins for Site-Directed Spin Labeling. Methods Enzymol (2015) 0.77

Methods to Make Homogenous Antibody Drug Conjugates. Pharm Res (2014) 0.76

Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue. Nat Commun (2016) 0.76

Site Specific Genetic Incorporation of Azidophenylalanine in Schizosaccharomyces pombe. Sci Rep (2015) 0.76

Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein. Sci Rep (2016) 0.75

Traceless native protein labeling in mice. Nat Chem Biol (2009) 0.75

Discrimination of colon cancer stem cells using noncanonical amino acid. Chem Commun (Camb) (2012) 0.75

Focal calcium monitoring with targeted nanosensors at the cytosolic side of endoplasmic reticulum. Sci Technol Adv Mater (2016) 0.75

The unnatural culture of amino acids. Nat Methods (2007) 0.75

Stress Response and Adaptation Mediated by Amino Acid Misincorporation during Protein Synthesis. Adv Nutr (2016) 0.75

Unexpected transcellular protein crossover occurs during canonical DNA transfection. J Cell Biochem (2014) 0.75

Selective in vivo metabolic cell-labeling-mediated cancer targeting. Nat Chem Biol (2017) 0.75

Antibody microarrays utilizing site-specific antibody-oligonucleotide conjugates. Bioconjug Chem (2015) 0.75

Genetically encoding new bioreactivity. N Biotechnol (2016) 0.75

Monitoring the assembly of a secreted bacterial virulence factor using site-specific crosslinking. J Vis Exp (2013) 0.75

Expanding the genetic code of Mus musculus. Nat Commun (2017) 0.75

Preparation of a Trp-BODIPY fluorogenic amino acid to label peptides for enhanced live-cell fluorescence imaging. Nat Protoc (2017) 0.75

Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs). Proteome Sci (2017) 0.75

Large-scale analysis of the human and mouse transcriptomes. Proc Natl Acad Sci U S A (2002) 25.47

Coordinated transcription of key pathways in the mouse by the circadian clock. Cell (2002) 14.07

Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat Biotechnol (2008) 10.29

Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines. Nature (2007) 7.12

Adding new chemistries to the genetic code. Annu Rev Biochem (2010) 6.32

Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline. Proc Natl Acad Sci U S A (2002) 6.00

Metabolomics analysis reveals large effects of gut microflora on mammalian blood metabolites. Proc Natl Acad Sci U S A (2009) 5.86

Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells. Science (2010) 5.50

Genomic analysis of the host response to hepatitis C virus infection. Proc Natl Acad Sci U S A (2002) 5.28

In silico activity profiling reveals the mechanism of action of antimalarials discovered in a high-throughput screen. Proc Natl Acad Sci U S A (2008) 4.80

An expanded eukaryotic genetic code. Science (2003) 4.66

Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc Natl Acad Sci U S A (2006) 3.90

Identification of NVP-TAE684, a potent, selective, and efficacious inhibitor of NPM-ALK. Proc Natl Acad Sci U S A (2006) 3.83

Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli. Proc Natl Acad Sci U S A (2002) 3.77

The renewal and differentiation of Isl1+ cardiovascular progenitors are controlled by a Wnt/beta-catenin pathway. Cell Stem Cell (2007) 3.54

Generation of pluripotent stem cells from patients with type 1 diabetes. Proc Natl Acad Sci U S A (2009) 3.43

Expanding the genetic code. Annu Rev Biophys Biomol Struct (2006) 3.36

Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell (2009) 3.31

Gene expression signatures and small-molecule compounds link a protein kinase to Plasmodium falciparum motility. Nat Chem Biol (2008) 3.23

A stem cell-based approach to cartilage repair. Science (2012) 3.22

An enhanced system for unnatural amino acid mutagenesis in E. coli. J Mol Biol (2009) 3.20

The role of PTEN/Akt/PI3K signaling in the maintenance and viability of prostate cancer stem-like cell populations. Proc Natl Acad Sci U S A (2008) 3.13

Imaging of Plasmodium liver stages to drive next-generation antimalarial drug discovery. Science (2011) 3.07

Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli. J Am Chem Soc (2002) 2.88

Addition of the keto functional group to the genetic code of Escherichia coli. Proc Natl Acad Sci U S A (2002) 2.84

Reprogramming of murine fibroblasts to induced pluripotent stem cells with chemical complementation of Klf4. Proc Natl Acad Sci U S A (2009) 2.80

Expanding the genetic code. Angew Chem Int Ed Engl (2004) 2.78

Genomically recoded organisms expand biological functions. Science (2013) 2.73

Self-renewal of embryonic stem cells by a small molecule. Proc Natl Acad Sci U S A (2006) 2.65

Prevalence and costs of chronic conditions in the VA health care system. Med Care Res Rev (2003) 2.52

An efficient rapid system for profiling the cellular activities of molecular libraries. Proc Natl Acad Sci U S A (2006) 2.51

Targeting Wnt-driven cancer through the inhibition of Porcupine by LGK974. Proc Natl Acad Sci U S A (2013) 2.49

Genome-scale functional profiling of the mammalian AP-1 signaling pathway. Proc Natl Acad Sci U S A (2003) 2.45

A chemical biology approach reveals period shortening of the mammalian circadian clock by specific inhibition of GSK-3beta. Proc Natl Acad Sci U S A (2008) 2.39

A chemical toolkit for proteins--an expanded genetic code. Nat Rev Mol Cell Biol (2006) 2.39

Dentin matrix protein 1, a target molecule for Cbfa1 in bone, is a unique bone marker gene. J Bone Miner Res (2002) 2.36

Recombinant expression of selectively sulfated proteins in Escherichia coli. Nat Biotechnol (2006) 2.31

Genome-wide functional analysis of human cell-cycle regulators. Proc Natl Acad Sci U S A (2006) 2.31

Efficient incorporation of unnatural amino acids into proteins in Escherichia coli. Nat Methods (2006) 2.24

Substrate recognition by the AAA+ chaperone ClpB. Nat Struct Mol Biol (2004) 2.19

Oxysterols direct immune cell migration via EBI2. Nature (2011) 2.19

Platinum-gold nanoparticles: a highly active bifunctional electrocatalyst for rechargeable lithium-air batteries. J Am Chem Soc (2010) 2.10

An expanded genetic code with a functional quadruplet codon. Proc Natl Acad Sci U S A (2004) 2.09

Synthetic small molecules that control stem cell fate. Proc Natl Acad Sci U S A (2003) 2.06

A facile system for encoding unnatural amino acids in mammalian cells. Angew Chem Int Ed Engl (2009) 2.02

Control of protein phosphorylation with a genetically encoded photocaged amino acid. Nat Chem Biol (2007) 1.99

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids. Proc Natl Acad Sci U S A (2012) 1.99

Identification of small molecule activators of cryptochrome. Science (2012) 1.95

A role for chemistry in stem cell biology. Nat Biotechnol (2004) 1.95

A genetically encoded fluorescent amino acid. J Am Chem Soc (2006) 1.93

The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination. Nat Biotechnol (2004) 1.93

Adding amino acids with novel reactivity to the genetic code of Saccharomyces cerevisiae. J Am Chem Soc (2003) 1.93

Exploring the limits of codon and anticodon size. Chem Biol (2002) 1.89

Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex. Science (2008) 1.79

A combinatorial scaffold approach toward kinase-directed heterocycle libraries. J Am Chem Soc (2002) 1.79

A small interfering RNA screen for modulators of tumor cell motility identifies MAP4K4 as a promigratory kinase. Proc Natl Acad Sci U S A (2006) 1.78

A small molecule primes embryonic stem cells for differentiation. Cell Stem Cell (2009) 1.78

A general approach to site-specific antibody drug conjugates. Proc Natl Acad Sci U S A (2014) 1.77

Directed evolution of the site specificity of Cre recombinase. Proc Natl Acad Sci U S A (2002) 1.77

Profiling protein function with small molecule microarrays. Proc Natl Acad Sci U S A (2002) 1.77

Slit2-Robo4 signalling promotes vascular stability by blocking Arf6 activity. Nat Cell Biol (2009) 1.73

An efficient system for the evolution of aminoacyl-tRNA synthetase specificity. Nat Biotechnol (2002) 1.73

A genetically encoded metabolically stable analogue of phosphotyrosine in Escherichia coli. ACS Chem Biol (2007) 1.72

A genetically encoded infrared probe. J Am Chem Soc (2006) 1.71

An approach to genomewide screens of expressed small interfering RNAs in mammalian cells. Proc Natl Acad Sci U S A (2003) 1.71

High-power lithium batteries from functionalized carbon-nanotube electrodes. Nat Nanotechnol (2010) 1.69

Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry. Proc Natl Acad Sci U S A (2003) 1.68

Progress toward an expanded eukaryotic genetic code. Chem Biol (2003) 1.66

A genetically encoded fluorescent amino acid. Proc Natl Acad Sci U S A (2006) 1.66

Identification of human kinases involved in hepatitis C virus replication by small interference RNA library screening. J Biol Chem (2007) 1.65

Site-directed spin labeling of a genetically encoded unnatural amino acid. Proc Natl Acad Sci U S A (2009) 1.65

A genetically encoded photocaged amino acid. J Am Chem Soc (2004) 1.65

A small-molecule inducer of the antioxidant response element. Chem Biol (2010) 1.65

Identification of a novel protein regulating microtubule stability through a chemical approach. Chem Biol (2004) 1.64

Small molecules that induce cardiomyogenesis in embryonic stem cells. J Am Chem Soc (2004) 1.63

Tom40 protein import channel binds to non-native proteins and prevents their aggregation. Nat Struct Biol (2003) 1.62

Generation of a bacterium with a 21 amino acid genetic code. J Am Chem Soc (2003) 1.62

Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway. Chem Biol (2004) 1.60

Enrichment and analysis of peptide subsets using fluorous affinity tags and mass spectrometry. Nat Biotechnol (2005) 1.59

A genetically encoded bidentate, metal-binding amino acid. Angew Chem Int Ed Engl (2007) 1.57

In vivo incorporation of unnatural amino acids to probe structure, dynamics, and ligand binding in a large protein by nuclear magnetic resonance spectroscopy. J Am Chem Soc (2008) 1.57

Automated immobilized metal affinity chromatography/nano-liquid chromatography/electrospray ionization mass spectrometry platform for profiling protein phosphorylation sites. Rapid Commun Mass Spectrom (2005) 1.56

Dedifferentiation of lineage-committed cells by a small molecule. J Am Chem Soc (2004) 1.56

A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer. J Am Chem Soc (2008) 1.56

A genetically encoded photocaged tyrosine. Angew Chem Int Ed Engl (2006) 1.56

A genetically encoded boronate-containing amino acid. Angew Chem Int Ed Engl (2008) 1.54

Site-specific insertion of 3-aminotyrosine into subunit alpha2 of E. coli ribonucleotide reductase: direct evidence for involvement of Y730 and Y731 in radical propagation. J Am Chem Soc (2007) 1.54

Coherent phonon heat conduction in superlattices. Science (2012) 1.53

Beyond the canonical 20 amino acids: expanding the genetic lexicon. J Biol Chem (2010) 1.53

A new strategy for the site-specific modification of proteins in vivo. Biochemistry (2003) 1.52

Bispecific small molecule-antibody conjugate targeting prostate cancer. Proc Natl Acad Sci U S A (2013) 1.52

Enthalpy arrays. Proc Natl Acad Sci U S A (2004) 1.52

Identification of a small molecule with activity against drug-resistant and persistent tuberculosis. Proc Natl Acad Sci U S A (2013) 1.51

Gene expression during the priming phase of liver regeneration after partial hepatectomy in mice. Proc Natl Acad Sci U S A (2002) 1.51