Published in Biophys J on March 09, 2007
Sensors and regulators of intracellular pH. Nat Rev Mol Cell Biol (2009) 4.30
Molecular aspects of bacterial pH sensing and homeostasis. Nat Rev Microbiol (2011) 2.46
Molecular dynamics simulations of membrane channels and transporters. Curr Opin Struct Biol (2009) 1.58
Transport mechanism and pH regulation of the Na+/H+ antiporter NhaA from Escherichia coli: an electrophysiological study. J Biol Chem (2011) 1.31
Structure-based functional study reveals multiple roles of transmembrane segment IX and loop VIII-IX in NhaA Na+/H+ antiporter of Escherichia coli at physiological pH. J Biol Chem (2008) 1.03
Revealing the ligand binding site of NhaA Na+/H+ antiporter and its pH dependence. J Biol Chem (2012) 0.95
Transmembrane segment II of NhaA Na+/H+ antiporter lines the cation passage, and Asp65 is critical for pH activation of the antiporter. J Biol Chem (2009) 0.94
A model-structure of a periplasm-facing state of the NhaA antiporter suggests the molecular underpinnings of pH-induced conformational changes. J Biol Chem (2012) 0.92
Site-directed tryptophan fluorescence reveals two essential conformational changes in the Na+/H+ antiporter NhaA. Proc Natl Acad Sci U S A (2011) 0.88
Structural and functional analysis of transmembrane XI of the NHE1 isoform of the Na+/H+ exchanger. J Biol Chem (2009) 0.82
Differential effects of mutations on the transport properties of the Na+/H+ antiporter NhaA from Escherichia coli. J Biol Chem (2013) 0.80
Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis. PLoS One (2011) 0.80
Capturing Functional Motions of Membrane Channels and Transporters with Molecular Dynamics Simulation. J Comput Theor Nanosci (2010) 0.79
Identification and characterization of methylation-dependent/independent DNA regulatory elements in the human SLC9B1 gene. Gene (2015) 0.77
Computational study of the Na+/H + antiporter from Vibrio parahaemolyticus. J Mol Model (2010) 0.76
The Ec-NhaA antiporter switches from antagonistic to synergistic antiport upon a single point mutation. Sci Rep (2016) 0.75
VMD: visual molecular dynamics. J Mol Graph (1996) 117.02
A computational procedure for determining energetically favorable binding sites on biologically important macromolecules. J Med Chem (1985) 6.09
Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH. Nature (2005) 3.74
Molecular dynamics of the KcsA K(+) channel in a bilayer membrane. Biophys J (2000) 3.21
Structure, energetics, and dynamics of lipid-protein interactions: A molecular dynamics study of the gramicidin A channel in a DMPC bilayer. Proteins (1996) 3.14
Hypothesis about the function of membrane-buried proline residues in transport proteins. Proc Natl Acad Sci U S A (1986) 2.90
Alkaline pH homeostasis in bacteria: new insights. Biochim Biophys Acta (2005) 2.64
Molecular dynamics simulation of melittin in a dimyristoylphosphatidylcholine bilayer membrane. Biophys J (1998) 2.16
The changing face of the Na+/H+ exchanger, NHE1: structure, regulation, and cellular actions. Annu Rev Pharmacol Toxicol (2002) 2.15
Hinges, swivels and switches: the role of prolines in signalling via transmembrane alpha-helices. Trends Pharmacol Sci (2000) 2.08
Na(+)/H(+) antiporters. Biochim Biophys Acta (2001) 2.06
NhaA of Escherichia coli, as a model of a pH-regulated Na+/H+antiporter. Biochim Biophys Acta (2004) 1.51
Molecular physiology of Na+/H+ antiporters, key transporters in circulation of Na+ and H+ in cells. Biochim Biophys Acta (1994) 1.50
Proton-sodium stoichiometry of NhaA, an electrogenic antiporter from Escherichia coli. J Biol Chem (1993) 1.47
Atomic resolution structures of bacteriorhodopsin photocycle intermediates: the role of discrete water molecules in the function of this light-driven ion pump. Biochim Biophys Acta (2000) 1.26
Prokink: a protocol for numerical evaluation of helix distortions by proline. Protein Eng (2000) 1.24
Trans membrane domain IV is involved in ion transport activity and pH regulation of the NhaA-Na(+)/H(+) antiporter of Escherichia coli. Biochemistry (2002) 1.17
A pragmatic approach to structure based calculation of coupled proton and electron transfer in proteins. Biochim Biophys Acta (2000) 1.16
A point mutation (G338S) and its suppressor mutations affect both the pH response of the NhaA-Na+/H+ antiporter as well as the growth phenotype of Escherichia coli. J Biol Chem (1998) 1.13
Molecular dynamics simulations of the complex between human U1A protein and hairpin II of U1 small nuclear RNA and of free RNA in solution. Biophys J (1999) 1.12
Mutation E252C increases drastically the Km value for Na+ and causes an alkaline shift of the pH dependence of NhaA Na+/H+ antiporter of Escherichia coli. J Biol Chem (2003) 1.09
Multiconformation continuum electrostatics analysis of the NhaA Na+/H+ antiporter of Escherichia coli with functional implications. Proc Natl Acad Sci U S A (2006) 1.08
Unraveling functional and structural interactions between transmembrane domains IV and XI of NhaA Na+/H+ antiporter of Escherichia coli. J Biol Chem (2004) 1.04
The monoclonal antibody 1F6 identifies a pH-dependent conformational change in the hydrophilic NH(2) terminus of NhaA Na(+)/H(+) antiporter of Escherichia coli. J Biol Chem (2000) 0.97
The role of extra-membranous inter-helical loops in helix-helix interactions. Protein Eng Des Sel (2005) 0.87
Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH. Nature (2005) 3.74
Overcoming barriers to membrane protein structure determination. Nat Biotechnol (2011) 2.49
Molecular aspects of bacterial pH sensing and homeostasis. Nat Rev Microbiol (2011) 2.46
The structure of cbb3 cytochrome oxidase provides insights into proton pumping. Science (2010) 1.68
Crystallisation of membrane proteins mediated by antibody fragments. Curr Opin Struct Biol (2002) 1.50
Alkalitolerance: a biological function for a multidrug transporter in pH homeostasis. Proc Natl Acad Sci U S A (2004) 1.46
Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Sci (2006) 1.46
Do physiological roles foster persistence of drug/multidrug-efflux transporters? A case study. Nat Rev Microbiol (2005) 1.36
Direct observation of tiers in the energy landscape of a chromoprotein: a single-molecule study. Proc Natl Acad Sci U S A (2003) 1.33
Model structure of the Na+/H+ exchanger 1 (NHE1): functional and clinical implications. J Biol Chem (2007) 1.32
Transport mechanism and pH regulation of the Na+/H+ antiporter NhaA from Escherichia coli: an electrophysiological study. J Biol Chem (2011) 1.31
The structure of Aquifex aeolicus sulfide:quinone oxidoreductase, a basis to understand sulfide detoxification and respiration. Proc Natl Acad Sci U S A (2009) 1.26
Dynamic water networks in cytochrome C oxidase from Paracoccus denitrificans investigated by molecular dynamics simulations. Biophys J (2004) 1.26
Reduction of cytochrome c oxidase by a second electron leads to proton translocation. Nature (2002) 1.25
Assessing oligomerization of membrane proteins by four-pulse DEER: pH-dependent dimerization of NhaA Na+/H+ antiporter of E. coli. Biophys J (2005) 1.24
Comparative analysis and "expression space" coverage of the production of prokaryotic membrane proteins for structural genomics. Protein Sci (2006) 1.21
High resolution crystal structure of Paracoccus denitrificans cytochrome c oxidase: new insights into the active site and the proton transfer pathways. Biochim Biophys Acta (2009) 1.20
Single-molecule study of the electronic couplings in a circular array of molecules: light-harvesting-2 complex from Rhodospirillum molischianum. Phys Rev Lett (2003) 1.20
Trans membrane domain IV is involved in ion transport activity and pH regulation of the NhaA-Na(+)/H(+) antiporter of Escherichia coli. Biochemistry (2002) 1.17
Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis. Structure (2007) 1.10
Mutation E252C increases drastically the Km value for Na+ and causes an alkaline shift of the pH dependence of NhaA Na+/H+ antiporter of Escherichia coli. J Biol Chem (2003) 1.09
Multiconformation continuum electrostatics analysis of the NhaA Na+/H+ antiporter of Escherichia coli with functional implications. Proc Natl Acad Sci U S A (2006) 1.08
Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems. J Struct Funct Genomics (2006) 1.08
The MAM (meprin/A5-protein/PTPmu) domain is a homophilic binding site promoting the lateral dimerization of receptor-like protein-tyrosine phosphatase mu. J Biol Chem (2004) 1.08
A new structure-based classification of sulfide:quinone oxidoreductases. Proteins (2010) 1.05
A D-pathway mutation decouples the Paracoccus denitrificans cytochrome c oxidase by altering the side-chain orientation of a distant conserved glutamate. J Mol Biol (2008) 1.05
Unraveling functional and structural interactions between transmembrane domains IV and XI of NhaA Na+/H+ antiporter of Escherichia coli. J Biol Chem (2004) 1.04
Monomers of the NhaA Na+/H+ antiporter of Escherichia coli are fully functional yet dimers are beneficial under extreme stress conditions at alkaline pH in the presence of Na+ or Li+. J Biol Chem (2007) 1.04
Structure-based functional study reveals multiple roles of transmembrane segment IX and loop VIII-IX in NhaA Na+/H+ antiporter of Escherichia coli at physiological pH. J Biol Chem (2008) 1.03
Roles of NhaA, NhaB, and NhaD Na+/H+ antiporters in survival of Vibrio cholerae in a saline environment. J Bacteriol (2003) 1.02
Titration behavior of residues at the entrance of the D-pathway of cytochrome c oxidase from paracoccus denitrificans investigated by continuum electrostatic calculations. Biophys J (2005) 1.02
Functional and structural interactions of the transmembrane domain X of NhaA, Na+/H+ antiporter of Escherichia coli, at physiological pH. Biochemistry (2007) 0.98
Beta-sheet-dependent dimerization is essential for the stability of NhaA Na+/H+ antiporter. J Biol Chem (2009) 0.97
Isolation, characterization and electron microscopic single particle analysis of the NADH:ubiquinone oxidoreductase (complex I) from the hyperthermophilic eubacterium Aquifex aeolicus. Biochemistry (2003) 0.97
Crystallization and preliminary X-ray crystallographic analysis of strictosidine synthase from Rauvolfia: the first member of a novel enzyme family. Biochim Biophys Acta (2004) 0.97
Tyrosine 167: the origin of the radical species observed in the reaction of cytochrome c oxidase with hydrogen peroxide in Paracoccus denitrificans. Biochemistry (2004) 0.95
Crucial steps in the structure determination of the Na+/H+ antiporter NhaA in its native conformation. J Mol Biol (2006) 0.95
Model-guided mutagenesis drives functional studies of human NHA2, implicated in hypertension. J Mol Biol (2010) 0.95
Revealing the ligand binding site of NhaA Na+/H+ antiporter and its pH dependence. J Biol Chem (2012) 0.95
Editorial: the nonsense of biofuels. Angew Chem Int Ed Engl (2012) 0.94
Heterologous expression and comparative characterization of the human neuromedin U subtype II receptor using the methylotrophic yeast Pichia pastoris and mammalian cells. Int J Biochem Cell Biol (2007) 0.94
Transmembrane segment II of NhaA Na+/H+ antiporter lines the cation passage, and Asp65 is critical for pH activation of the antiporter. J Biol Chem (2009) 0.94
Expression, purification, crystallization and preliminary X-ray analysis of strictosidine glucosidase, an enzyme initiating biosynthetic pathways to a unique diversity of indole alkaloid skeletons. Biochim Biophys Acta (2004) 0.92
A model-structure of a periplasm-facing state of the NhaA antiporter suggests the molecular underpinnings of pH-induced conformational changes. J Biol Chem (2012) 0.92
Dimethylsulphoxide as a tool to increase functional expression of heterologously produced GPCRs in mammalian cells. FEBS Lett (2006) 0.91
Heterologous expression of human Neuromedin U receptor 1 and its subsequent solubilization and purification. Biochim Biophys Acta (2008) 0.91
Crystallization and preliminary X-ray analysis of strictosidine synthase and its complex with the substrate tryptamine. Acta Crystallogr D Biol Crystallogr (2005) 0.89
The role of tryptophan 272 in the Paracoccus denitrificans cytochrome c oxidase. FEBS Lett (2006) 0.89
Multivariate analysis of single-molecule spectra: surpassing spectral diffusion. Phys Rev Lett (2005) 0.89
The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter. Biochim Biophys Acta (2012) 0.88
Site-directed tryptophan fluorescence reveals two essential conformational changes in the Na+/H+ antiporter NhaA. Proc Natl Acad Sci U S A (2011) 0.88
Employing Rhodobacter sphaeroides to functionally express and purify human G protein-coupled receptors. Biol Chem (2008) 0.88
Vibrational modes of tyrosines in cytochrome c oxidase from Paracoccus denitrificans: FTIR and electrochemical studies on Tyr-D4-labeled and on Tyr280His and Tyr35Phe mutant enzymes. Biochemistry (2002) 0.87
Biochemical and biophysical characterization of the two isoforms of cbb3-type cytochrome c oxidase from Pseudomonas stutzeri. J Bacteriol (2013) 0.87
A L-lysine transporter of high stereoselectivity of the amino acid-polyamine-organocation (APC) superfamily: production, functional characterization, and structure modeling. J Biol Chem (2013) 0.85
Conformational changes in NhaA Na+/H+ antiporter. Mol Membr Biol (2012) 0.85
Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation. FEBS J (2007) 0.85
Characterizing a monotopic membrane enzyme. Biochemical, enzymatic and crystallization studies on Aquifex aeolicus sulfide:quinone oxidoreductase. Biochim Biophys Acta (2010) 0.83
Rates and Equilibrium of CuA to heme a electron transfer in Paracoccus denitrificans cytochrome c oxidase. Biophys J (2005) 0.83
The structure of the neuropeptide bradykinin bound to the human G-protein coupled receptor bradykinin B2 as determined by solid-state NMR spectroscopy. Angew Chem Int Ed Engl (2008) 0.83
Production of the human D2S receptor in the methylotrophic yeast P. pastoris. Receptors Channels (2004) 0.83
Elucidating mechanisms in haem copper oxidases: the high-affinity QH binding site in quinol oxidase as studied by DONUT-HYSCORE spectroscopy and density functional theory. Faraday Discuss (2011) 0.82
Net proton uptake is preceded by multiple proton transfer steps upon electron injection into cytochrome c oxidase. J Biol Chem (2012) 0.82
Helix VIII of NhaA Na(+)/H(+) antiporter participates in the periplasmic cation passage and pH regulation of the antiporter. J Mol Biol (2011) 0.82
Comparative analysis of the human angiotensin II type 1a receptor heterologously produced in insect cells and mammalian cells. Biochem Biophys Res Commun (2006) 0.81
2-Aminoperimidine, a specific inhibitor of bacterial NhaA Na(+)/H(+) antiporters. FEBS Lett (2005) 0.81
Vinorine synthase from Rauvolfia: the first example of crystallization and preliminary X-ray diffraction analysis of an enzyme of the BAHD superfamily. Biochim Biophys Acta (2004) 0.81
Structure at 1.3 A resolution of Rhodothermus marinus caa(3) cytochrome c domain. J Mol Biol (2004) 0.80
The unwound portion dividing helix IV of NhaA undergoes a conformational change at physiological pH and lines the cation passage. Biochemistry (2012) 0.80
Differential effects of mutations on the transport properties of the Na+/H+ antiporter NhaA from Escherichia coli. J Biol Chem (2013) 0.80
Epitope mapping of conformational monoclonal antibodies specific to NhaA Na+/H+ antiporter: structural and functional implications. J Mol Biol (2008) 0.80
Low-intensity pump-probe measurements on the B800 band of Rhodospirillum molischianum. Biophys J (2003) 0.79
Biochemical and electron microscopic characterization of the F1F0 ATP synthase from the hyperthermophilic eubacterium Aquifex aeolicus. FEBS Lett (2006) 0.79
Two conflicting NHE1 model structures: compatibility with experimental data and implications for the transport mechanism. J Biol Chem (2011) 0.79
Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system. Biol Chem (2006) 0.78
Heterologous production and functional and thermodynamic characterization of cation diffusion facilitator (CDF) transporters of mesophilic and hyperthermophilic origin. Biol Chem (2012) 0.78
Interconversions of P and F intermediates of cytochrome c oxidase from Paracoccus denitrificans. Proc Natl Acad Sci U S A (2011) 0.78
C-terminal truncated cannabinoid receptor 1 coexpressed with G protein trimer in Sf9 cells exists in a precoupled state and shows constitutive activity. FEBS J (2007) 0.78
True wild type and recombinant wild type cytochrome c oxidase from Paracoccus denitrificans show a 20-fold difference in their catalase activity. Biochim Biophys Acta (2012) 0.78
Functional overexpression and characterization of human bradykinin subtype 2 receptor in insect cells using the baculovirus system. J Cell Biochem (2006) 0.77
Comparative analysis of high-affinity ligand binding and G protein coupling of the human CXCR1 chemokine receptor and of a CXCR1-Galpha fusion protein after heterologous production in baculovirus-infected insect cells. Eur J Biochem (2004) 0.77
Structure at 1.5 A resolution of cytochrome c(552) with its flexible linker segment, a membrane-anchored protein from Paracoccus denitrificans. Acta Crystallogr D Biol Crystallogr (2010) 0.77
The ESFRI Instruct Core Centre Frankfurt: automated high-throughput crystallization suited for membrane proteins and more. J Struct Funct Genomics (2011) 0.76
1.3 A X-ray structure of an antibody Fv fragment used for induced membrane-protein crystallization. Acta Crystallogr D Biol Crystallogr (2003) 0.76
A decade of crystallization drops: crystallization of the cbb3 cytochrome c oxidase from Pseudomonas stutzeri. Protein Sci (2014) 0.76
Exploring the entrance of proton pathways in cytochrome c oxidase from Paracoccus denitrificans: surface charge, buffer capacity and redox-dependent polarity changes at the internal surface. Biochim Biophys Acta (2012) 0.76
Heterologous expression and characterization of the recombinant bradykinin B2 receptor using the methylotrophic yeast Pichia pastoris. Protein Expr Purif (2007) 0.76
Protein AQ_1862 from the hyperthermophilic bacterium Aquifex aeolicus is a porin and contains two conductance pathways of different selectivity. Biophys J (2007) 0.76
Evidence for distinct electron transfer processes in terminal oxidases from different origin by means of protein film voltammetry. J Am Chem Soc (2014) 0.75
Heme-copper terminal oxidase using both cytochrome c and ubiquinol as electron donors. Proc Natl Acad Sci U S A (2012) 0.75
Resonance Raman characterization of the ammonia-generated oxo intermediate of cytochrome c oxidase from Paracoccus denitrificans. Biochemistry (2013) 0.75
Production of fully assembled and active Aquifex aeolicus F1FO ATP synthase in Escherichia coli. Biochim Biophys Acta (2013) 0.75
Isolation, functional characterization and crystallization of Aq_1259, an outer membrane protein with porin features, from Aquifex aeolicus. Biochim Biophys Acta (2012) 0.75