Published in Chembiochem on December 17, 2007
An efficient method to assemble linear DNA templates for in vitro screening and selection systems. Nucleic Acids Res (2009) 0.98
Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution. Nucleic Acids Res (2012) 0.90
In vitro affinity screening of protein and peptide binders by megavalent bead surface display. Protein Eng Des Sel (2013) 0.89
Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process. Protein Eng Des Sel (2015) 0.79
Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification. Nucleic Acids Res (2010) 0.79
Bioinspired genotype-phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins. Interface Focus (2015) 0.78
A Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis. Sci Rep (2016) 0.75
Selection platforms for directed evolution in synthetic biology. Biochem Soc Trans (2016) 0.75
A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotechnol (2002) 6.77
An engineered protein tag for multiprotein labeling in living cells. Chem Biol (2008) 3.92
Labeling of fusion proteins with synthetic fluorophores in live cells. Proc Natl Acad Sci U S A (2004) 3.11
Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angew Chem Int Ed Engl (2010) 2.61
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nat Chem (2013) 2.61
Microdroplets: a sea of applications? Lab Chip (2008) 2.55
Specific labeling of cell surface proteins with chemically diverse compounds. J Am Chem Soc (2004) 2.27
FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells. Proc Natl Acad Sci U S A (2006) 2.04
Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution. Chem Biol (2012) 1.87
How to obtain labeled proteins and what to do with them. Curr Opin Biotechnol (2010) 1.85
Cross-talk between histone modifications in response to histone deacetylase inhibitors: MLL4 links histone H3 acetylation and histone H3K4 methylation. J Biol Chem (2006) 1.82
Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo. Chem Biol (2003) 1.76
Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays. Lab Chip (2008) 1.59
Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution. Chem Biol (2004) 1.52
Identification of a small molecule with activity against drug-resistant and persistent tuberculosis. Proc Natl Acad Sci U S A (2013) 1.51
Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging. Chembiochem (2011) 1.51
Regulation of glutamate metabolism by protein kinases in mycobacteria. Mol Microbiol (2008) 1.48
Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro. Methods (2004) 1.46
Directed evolution of a gatekeeper domain in nonribosomal peptide synthesis. Chem Biol (2011) 1.45
A yeast-based screen reveals that sulfasalazine inhibits tetrahydrobiopterin biosynthesis. Nat Chem Biol (2011) 1.42
Chemical probes shed light on protein function. Curr Opin Struct Biol (2007) 1.42
Chemical tools for biomolecular imaging. ACS Chem Biol (2007) 1.41
Benzothiazinones: prodrugs that covalently modify the decaprenylphosphoryl-β-D-ribose 2'-epimerase DprE1 of Mycobacterium tuberculosis. J Am Chem Soc (2010) 1.35
Benzothiazinones are suicide inhibitors of mycobacterial decaprenylphosphoryl-β-D-ribofuranose 2'-oxidase DprE1. J Am Chem Soc (2011) 1.33
Development of quantitative cell-based enzyme assays in microdroplets. Anal Chem (2008) 1.29
Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 centrosomal complex. Curr Biol (2013) 1.19
Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets. Anal Chem (2009) 1.19
Photoactivatable and photoconvertible fluorescent probes for protein labeling. ACS Chem Biol (2010) 1.18
What makes an enzyme promiscuous? Curr Opin Chem Biol (2010) 1.17
Controlling the retention of small molecules in emulsion microdroplets for use in cell-based assays. Anal Chem (2009) 1.13
Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments. J Am Chem Soc (2009) 1.11
The potential of microfluidic water-in-oil droplets in experimental biology. Mol Biosyst (2009) 1.08
An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily. Proc Natl Acad Sci U S A (2010) 1.07
Adding value to fusion proteins through covalent labelling. Curr Opin Biotechnol (2005) 1.07
An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets. Chembiochem (2008) 1.06
Protein chemistry on the surface of living cells. Chembiochem (2005) 1.04
Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling. Protein Eng Des Sel (2006) 1.04
A double droplet trap system for studying mass transport across a droplet-droplet interface. Lab Chip (2010) 1.04
Indo-1 derivatives for local calcium sensing. ACS Chem Biol (2009) 1.03
Microfluidic droplets: new integrated workflows for biological experiments. Curr Opin Chem Biol (2010) 1.03
Multicolor imaging of cell surface proteins. J Am Chem Soc (2005) 1.02
Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma. Hepatology (2012) 1.01
Covalent and selective immobilization of fusion proteins. J Am Chem Soc (2003) 1.01
A Trojan horse transition state analogue generated by MgF3- formation in an enzyme active site. Proc Natl Acad Sci U S A (2006) 1.00
Induced protein dimerization in vivo through covalent labeling. J Am Chem Soc (2003) 0.99
Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays. ACS Nano (2013) 0.99
New genotype-phenotype linkages for directed evolution of functional proteins. Curr Opin Struct Biol (2005) 0.98
The human histone acetyltransferase P/CAF is a promiscuous histone propionyltransferase. Chembiochem (2008) 0.98
One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution. Anal Chem (2014) 0.98
An efficient method to assemble linear DNA templates for in vitro screening and selection systems. Nucleic Acids Res (2009) 0.98
Efficient catalytic promiscuity in an enzyme superfamily: an arylsulfatase shows a rate acceleration of 10(13) for phosphate monoester hydrolysis. J Am Chem Soc (2008) 0.98
A caged, localizable rhodamine derivative for superresolution microscopy. ACS Chem Biol (2011) 0.98
Anionic charge is prioritized over geometry in aluminum and magnesium fluoride transition state analogs of phosphoryl transfer enzymes. J Am Chem Soc (2008) 0.97
Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation. N Biotechnol (2012) 0.97
Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method. J Am Chem Soc (2011) 0.97
An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets. Lab Chip (2009) 0.97
Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells. Chembiochem (2005) 0.96
Selective cross-linking of interacting proteins using self-labeling tags. J Am Chem Soc (2009) 0.96
Semisynthetic fluorescent sensor proteins based on self-labeling protein tags. J Am Chem Soc (2009) 0.95
Efficient catalytic promiscuity for chemically distinct reactions. Angew Chem Int Ed Engl (2009) 0.95
Measuring in vivo protein half-life. Chem Biol (2011) 0.92
Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging. Lab Chip (2009) 0.92
Chapter 4. Interactions of chemokines with glycosaminoglycans. Methods Enzymol (2009) 0.92
Semisynthesis of fluorescent metabolite sensors on cell surfaces. J Am Chem Soc (2011) 0.91
Phosphopantetheinyl transferase-catalyzed formation of bioactive hydrogels for tissue engineering. J Am Chem Soc (2010) 0.91
A fluorescent sensor for GABA and synthetic GABA(B) receptor ligands. J Am Chem Soc (2012) 0.91
A split-protein sensor for studying protein-protein interaction in mycobacteria. J Microbiol Methods (2008) 0.90
A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red. ACS Chem Biol (2014) 0.89
In vitro affinity screening of protein and peptide binders by megavalent bead surface display. Protein Eng Des Sel (2013) 0.89
A single mutation in the core domain of the lac repressor reduces leakiness. Microb Cell Fact (2013) 0.89
A fusion of disciplines: chemical approaches to exploit fusion proteins for functional genomics. Chembiochem (2003) 0.89
Efficient, crosswise catalytic promiscuity among enzymes that catalyze phosphoryl transfer. Biochim Biophys Acta (2012) 0.88
A simple method to evaluate the biochemical compatibility of oil/surfactant mixtures for experiments in microdroplets. Lab Chip (2012) 0.88
A designed protein for the specific and covalent heteroconjugation of biomolecules. Bioconjug Chem (2008) 0.88
Simultaneous measurement of reactions in microdroplets filled by concentration gradients. Lab Chip (2009) 0.88
WhiB5, a transcriptional regulator that contributes to Mycobacterium tuberculosis virulence and reactivation. Infect Immun (2012) 0.87
Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5). Biophys J (2010) 0.87
Mapping the limits of substrate specificity of the adenylation domain of TycA. Chembiochem (2009) 0.86
Evolving the substrate specificity of O6-alkylguanine-DNA alkyltransferase through loop insertion for applications in molecular imaging. ACS Chem Biol (2006) 0.86
Synthesis and characterization of bifunctional probes for the specific labeling of fusion proteins. Bioorg Med Chem Lett (2004) 0.86
Searching for the protein targets of bioactive molecules. Chimia (Aarau) (2011) 0.86
Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability. Biochemistry (2012) 0.85
A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition. Anal Chem (2013) 0.85
Targeted photoswitchable probe for nanoscopy of biological structures. Chembiochem (2010) 0.85
Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore. Anal Chem (2010) 0.84
Caged substrates for protein labeling and immobilization. Chembiochem (2008) 0.84
An optimized ATP/PP(i)-exchange assay in 96-well format for screening of adenylation domains for applications in combinatorial biosynthesis. Biotechnol J (2007) 0.83
Controlling the contents of microdroplets by exploiting the permeability of PDMS. Lab Chip (2011) 0.82
Droplets as reaction compartments for protein nanotechnology. Methods Mol Biol (2013) 0.82
A semisynthetic fluorescent sensor protein for glutamate. J Am Chem Soc (2012) 0.82
Kinetic analysis of beta-phosphoglucomutase and its inhibition by magnesium fluoride. J Am Chem Soc (2009) 0.82
Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag. Dev Dyn (2011) 0.82
Tetrahydrobiopterin biosynthesis as an off-target of sulfa drugs. Science (2013) 0.82
Peptide dendrimer/lipid hybrid systems are efficient DNA transfection reagents: structure--activity relationships highlight the role of charge distribution across dendrimer generations. ACS Nano (2013) 0.81
Protein function microarrays based on self-immobilizing and self-labeling fusion proteins. Chembiochem (2006) 0.81
Covalent labeling of cell-surface proteins for in-vivo FRET studies. FEBS Lett (2006) 0.81
Protein-functionalized polymer brushes. Biomacromolecules (2005) 0.81