Amide bonds assemble pili on the surface of bacilli.

PubWeight™: 1.53‹?› | Rank: Top 4%

🔗 View Article (PMC 2481347)

Published in Proc Natl Acad Sci U S A on July 11, 2008

Authors

Jonathan M Budzik1, Luciano A Marraffini, Puneet Souda, Julian P Whitelegge, Kym F Faull, Olaf Schneewind

Author Affiliations

1: Department of Microbiology, University of Chicago, Chicago, IL 60637, USA.

Articles citing this

An accessory protein required for anchoring and assembly of amyloid fibres in B. subtilis biofilms. Mol Microbiol (2011) 2.17

The Corynebacterium diphtheriae shaft pilin SpaA is built of tandem Ig-like modules with stabilizing isopeptide and disulfide bonds. Proc Natl Acad Sci U S A (2009) 1.79

Protein secretion and surface display in Gram-positive bacteria. Philos Trans R Soc Lond B Biol Sci (2012) 1.61

A tale of two pili: assembly and function of pili in bacteria. Trends Microbiol (2010) 1.47

Intramolecular isopeptide bonds give thermodynamic and proteolytic stability to the major pilin protein of Streptococcus pyogenes. J Biol Chem (2009) 1.45

Isopeptide bonds block the mechanical extension of pili in pathogenic Streptococcus pyogenes. J Biol Chem (2010) 1.38

Sortase enzymes in Gram-positive bacteria. Mol Microbiol (2011) 1.37

A straight path to circular proteins. J Biol Chem (2009) 1.31

Intramolecular amide bonds stabilize pili on the surface of bacilli. Proc Natl Acad Sci U S A (2009) 1.31

Sortase D forms the covalent bond that links BcpB to the tip of Bacillus cereus pili. J Biol Chem (2009) 1.25

A highly unusual thioester bond in a pilus adhesin is required for efficient host cell interaction. J Biol Chem (2010) 1.25

Mechanism for sortase localization and the role of sortase localization in efficient pilus assembly in Enterococcus faecalis. J Bacteriol (2009) 1.23

Two intramolecular isopeptide bonds are identified in the crystal structure of the Streptococcus gordonii SspB C-terminal domain. J Mol Biol (2010) 1.17

Crystal structure of the minor pilin FctB reveals determinants of Group A streptococcal pilus anchoring. J Biol Chem (2010) 1.16

The minor pilin subunit Sgp2 is necessary for assembly of the pilus encoded by the srtG cluster of Streptococcus suis. J Bacteriol (2010) 1.10

Cell wall anchor structure of BcpA pili in Bacillus anthracis. J Biol Chem (2008) 1.10

Structure-based approach to rationally design a chimeric protein for an effective vaccine against Group B Streptococcus infections. Proc Natl Acad Sci U S A (2011) 1.09

NMR spectroscopic and theoretical analysis of a spontaneously formed Lys-Asp isopeptide bond. Angew Chem Int Ed Engl (2010) 1.06

Acyl enzyme intermediates in sortase-catalyzed pilus morphogenesis in gram-positive bacteria. J Bacteriol (2009) 1.04

Sec-secretion and sortase-mediated anchoring of proteins in Gram-positive bacteria. Biochim Biophys Acta (2013) 1.03

Two autonomous structural modules in the fimbrial shaft adhesin FimA mediate Actinomyces interactions with streptococci and host cells during oral biofilm development. Mol Microbiol (2011) 1.03

Stability and assembly of pilus subunits of Streptococcus pneumoniae. J Biol Chem (2010) 1.02

Pilus biogenesis in Lactococcus lactis: molecular characterization and role in aggregation and biofilm formation. PLoS One (2012) 0.97

Sortases make pili from three ingredients. Proc Natl Acad Sci U S A (2008) 0.94

Structure of the full-length major pilin from Streptococcus pneumoniae: implications for isopeptide bond formation in gram-positive bacterial pili. PLoS One (2011) 0.93

Assembly mechanism of FCT region type 1 pili in serotype M6 Streptococcus pyogenes. J Biol Chem (2011) 0.91

Pilin and sortase residues critical for endocarditis- and biofilm-associated pilus biogenesis in Enterococcus faecalis. J Bacteriol (2013) 0.90

Expression, purification, crystallization and preliminary crystallographic analysis of SpaA, a major pilin from Corynebacterium diphtheriae. Acta Crystallogr Sect F Struct Biol Cryst Commun (2009) 0.82

Isopeptide bonds of the major pilin protein BcpA influence pilus structure and bundle formation on the surface of Bacillus cereus. Mol Microbiol (2012) 0.82

Structure of the C-terminal domain of AspA (antigen I/II-family) protein from Streptococcus pyogenes. FEBS Open Bio (2014) 0.78

Pilus backbone protein PitB of Streptococcus pneumoniae contains stabilizing intramolecular isopeptide bonds. Biochem Biophys Res Commun (2011) 0.76

Structure, Function, and Assembly of Adhesive Organelles by Uropathogenic Bacteria. Microbiol Spectr (2015) 0.75

The role of pili in Bacillus cereus intraocular infection. Exp Eye Res (2017) 0.75

Molecular Mechanisms of the Binding and Specificity of Streptococcus Pneumoniae Sortase C Enzymes for Pilin Subunits. Sci Rep (2017) 0.75

Articles cited by this

Current knowledge of type-specific M antigens of group A streptococci. J Immunol (1962) 14.71

Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall. Science (1999) 5.98

Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria. Microbiol Mol Biol Rev (2006) 4.88

A pneumococcal pilus influences virulence and host inflammatory responses. Proc Natl Acad Sci U S A (2006) 4.40

Structure of the cell wall anchor of surface proteins in Staphylococcus aureus. Science (1995) 4.27

Pili in gram-positive pathogens. Nat Rev Microbiol (2006) 4.21

Structure of a lipid intermediate in cell wall peptidoglycan synthesis: a derivative of a C55 isoprenoid alcohol. Proc Natl Acad Sci U S A (1967) 4.15

Stabilizing isopeptide bonds revealed in gram-positive bacterial pilus structure. Science (2007) 4.10

Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif. Proc Natl Acad Sci U S A (1999) 3.90

Genome analysis reveals pili in Group B Streptococcus. Science (2005) 3.85

Identification of a universal Group B streptococcus vaccine by multiple genome screen. Science (2005) 3.83

Assembly of pili on the surface of Corynebacterium diphtheriae. Mol Microbiol (2003) 3.79

Group A Streptococcus produce pilus-like structures containing protective antigens and Lancefield T antigens. Proc Natl Acad Sci U S A (2005) 3.59

Assembly and role of pili in group B streptococci. Mol Microbiol (2006) 2.92

Sortases and pilin elements involved in pilus assembly of Corynebacterium diphtheriae. Mol Microbiol (2004) 2.80

Assembly of pili in Gram-positive bacteria. Trends Microbiol (2004) 2.76

Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Sortase catalyzed in vitro transpeptidation reaction using LPXTG peptide and NH(2)-Gly(3) substrates. J Biol Chem (2000) 2.52

RrgA is a pilus-associated adhesin in Streptococcus pneumoniae. Mol Microbiol (2007) 2.01

Anchoring of surface proteins to the cell wall of Staphylococcus aureus. III. Lipid II is an in vivo peptidoglycan substrate for sortase-catalyzed surface protein anchoring. J Biol Chem (2002) 1.97

Novel fold and assembly of the repetitive B region of the Staphylococcus aureus collagen-binding surface protein. Structure (2000) 1.87

Housekeeping sortase facilitates the cell wall anchoring of pilus polymers in Corynebacterium diphtheriae. Mol Microbiol (2007) 1.82

Sequence and structural characteristics of the trypsin-resistant T6 surface protein of group A streptococci. J Bacteriol (1990) 1.78

Streptococcus pneumoniae pilus subunits protect mice against lethal challenge. Infect Immun (2006) 1.74

Sortase-catalyzed assembly of distinct heteromeric fimbriae in Actinomyces naeslundii. J Bacteriol (2007) 1.71

Anchor structure of staphylococcal surface proteins. A branched peptide that links the carboxyl terminus of proteins to the cell wall. J Biol Chem (1997) 1.68

Assembly of pili on the surface of Bacillus cereus vegetative cells. Mol Microbiol (2007) 1.67

An IgG-like domain in the minor pilin GBS52 of Streptococcus agalactiae mediates lung epithelial cell adhesion. Structure (2007) 1.64

Targeting proteins to the cell wall of sporulating Bacillus anthracis. Mol Microbiol (2006) 1.49

Cloning and nucleotide sequence of a gene for Actinomyces naeslundii WVU45 type 2 fimbriae. J Bacteriol (1988) 1.19

Articles by these authors

Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53

DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol (2013) 19.99

RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol (2013) 15.51

The minimum information about a proteomics experiment (MIAPE). Nat Biotechnol (2007) 10.24

Exogenous and endogenous glycolipid antigens activate NKT cells during microbial infections. Nature (2005) 7.72

Allelic replacement in Staphylococcus aureus with inducible counter-selection. Plasmid (2005) 6.11

Poring over pores: alpha-hemolysin and Panton-Valentine leukocidin in Staphylococcus aureus pneumonia. Nat Med (2007) 5.95

Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria. Microbiol Mol Biol Rev (2006) 4.88

Passage of heme-iron across the envelope of Staphylococcus aureus. Science (2003) 4.83

mzML--a community standard for mass spectrometry data. Mol Cell Proteomics (2010) 4.81

Iron-source preference of Staphylococcus aureus infections. Science (2004) 4.33

Targeting of alpha-hemolysin by active or passive immunization decreases severity of USA300 skin infection in a mouse model. J Infect Dis (2010) 4.19

Panton-Valentine leukocidin is not a virulence determinant in murine models of community-associated methicillin-resistant Staphylococcus aureus disease. J Infect Dis (2008) 4.16

Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands. J Bacteriol (2007) 4.07

Vaccine protection against Staphylococcus aureus pneumonia. J Exp Med (2008) 4.01

Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing. Proc Natl Acad Sci U S A (2004) 3.89

Assembly of pili on the surface of Corynebacterium diphtheriae. Mol Microbiol (2003) 3.79

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res (2013) 3.51

Surface proteins and exotoxins are required for the pathogenesis of Staphylococcus aureus pneumonia. Infect Immun (2006) 3.41

Staphylococcus aureus degrades neutrophil extracellular traps to promote immune cell death. Science (2013) 3.32

Plague bacteria target immune cells during infection. Science (2005) 3.32

Combined deficiency of Tet1 and Tet2 causes epigenetic abnormalities but is compatible with postnatal development. Dev Cell (2013) 3.31

An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis. Proc Natl Acad Sci U S A (2002) 3.23

Atomic view of a toxic amyloid small oligomer. Science (2012) 3.13

Genetic requirements for Staphylococcus aureus abscess formation and persistence in host tissues. FASEB J (2009) 2.82

Sortases and pilin elements involved in pilus assembly of Corynebacterium diphtheriae. Mol Microbiol (2004) 2.80

Assembly of pili in Gram-positive bacteria. Trends Microbiol (2004) 2.76

Vaccine assembly from surface proteins of Staphylococcus aureus. Proc Natl Acad Sci U S A (2006) 2.71

Staphylococcus aureus IsdB is a hemoglobin receptor required for heme iron utilization. J Bacteriol (2006) 2.54

The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions. J Proteome Res (2008) 2.53

Protein sorting to the cell wall envelope of Gram-positive bacteria. Biochim Biophys Acta (2004) 2.42

A broad-spectrum antiviral targeting entry of enveloped viruses. Proc Natl Acad Sci U S A (2010) 2.41

IsdG and IsdI, heme-degrading enzymes in the cytoplasm of Staphylococcus aureus. J Biol Chem (2003) 2.39

Iron-regulated surface determinants (Isd) of Staphylococcus aureus: stealing iron from heme. Microbes Infect (2004) 2.37

The Bacillus subtilis quorum-sensing molecule CSF contributes to intestinal homeostasis via OCTN2, a host cell membrane transporter. Cell Host Microbe (2007) 2.26

Prophages of Staphylococcus aureus Newman and their contribution to virulence. Mol Microbiol (2006) 2.20

Comparative analysis of USA300 virulence determinants in a rabbit model of skin and soft tissue infection. J Infect Dis (2011) 2.19

The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptors. Proc Natl Acad Sci U S A (2004) 2.19

Mature clustered, regularly interspaced, short palindromic repeats RNA (crRNA) length is measured by a ruler mechanism anchored at the precursor processing site. Proc Natl Acad Sci U S A (2011) 2.17

Metal-free superoxide dismutase forms soluble oligomers under physiological conditions: a possible general mechanism for familial ALS. Proc Natl Acad Sci U S A (2007) 2.16

CRISPR interference can prevent natural transformation and virulence acquisition during in vivo bacterial infection. Cell Host Microbe (2012) 2.15

Side-chain contributions to membrane protein structure and stability. J Mol Biol (2004) 2.13

Soluble factors from Lactobacillus GG activate MAPKs and induce cytoprotective heat shock proteins in intestinal epithelial cells. Am J Physiol Cell Physiol (2005) 2.07

Synthesis of glycerol phosphate lipoteichoic acid in Staphylococcus aureus. Proc Natl Acad Sci U S A (2007) 2.06

Characterization of serum biomarkers for detection of early stage ovarian cancer. Proteomics (2005) 2.06

Modest stabilization by most hydrogen-bonded side-chain interactions in membrane proteins. Nature (2008) 2.03

Anchoring of surface proteins to the cell wall of Staphylococcus aureus. III. Lipid II is an in vivo peptidoglycan substrate for sortase-catalyzed surface protein anchoring. J Biol Chem (2002) 1.97

Dealing with the evolutionary downside of CRISPR immunity: bacteria and beneficial plasmids. PLoS Genet (2013) 1.97

Substrate recognition by the Yersinia type III protein secretion machinery. Mol Microbiol (2003) 1.93

hPepT1 transports muramyl dipeptide, activating NF-kappaB and stimulating IL-8 secretion in human colonic Caco2/bbe cells. Gastroenterology (2004) 1.91

Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence. Mol Microbiol (2002) 1.89

Curcumin structure-function, bioavailability, and efficacy in models of neuroinflammation and Alzheimer's disease. J Pharmacol Exp Ther (2008) 1.86

Staphylococcus aureus synthesizes adenosine to escape host immune responses. J Exp Med (2009) 1.85

An oxidation-sensing mechanism is used by the global regulator MgrA in Staphylococcus aureus. Nat Chem Biol (2006) 1.83

Rapidly progressive, fatal, inhalation anthrax-like infection in a human: case report, pathogen genome sequencing, pathology, and coordinated response. Arch Pathol Lab Med (2011) 1.83

Architects at the bacterial surface - sortases and the assembly of pili with isopeptide bonds. Nat Rev Microbiol (2011) 1.79

A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat Cell Biol (2010) 1.79

Nontoxigenic protein A vaccine for methicillin-resistant Staphylococcus aureus infections in mice. J Exp Med (2010) 1.77

The structure of sortase B, a cysteine transpeptidase that tethers surface protein to the Staphylococcus aureus cell wall. Structure (2004) 1.76

Identification of proteins associated with murine gammaherpesvirus 68 virions. J Virol (2003) 1.75

Contribution of coagulases towards Staphylococcus aureus disease and protective immunity. PLoS Pathog (2010) 1.75

Initiation and elongation in fibrillation of ALS-linked superoxide dismutase. Proc Natl Acad Sci U S A (2008) 1.73

Cross-linked peptidoglycan mediates lysostaphin binding to the cell wall envelope of Staphylococcus aureus. J Bacteriol (2006) 1.72

Discovery and characterization of a unique mycobacterial heme acquisition system. Proc Natl Acad Sci U S A (2011) 1.71

Destabilization of apoprotein is insufficient to explain Cu,Zn-superoxide dismutase-linked ALS pathogenesis. Proc Natl Acad Sci U S A (2005) 1.67

Assembly of pili on the surface of Bacillus cereus vegetative cells. Mol Microbiol (2007) 1.67

Sortase as a target of anti-infective therapy. Pharmacol Rev (2008) 1.64

Iron acquisition and transport in Staphylococcus aureus. Biometals (2006) 1.61

Site-specific increases in peripheral cannabinoid receptors and their endogenous ligands in a model of neuropathic pain. Pain (2006) 1.61

BslA, a pXO1-encoded adhesin of Bacillus anthracis. Mol Microbiol (2008) 1.60

Crystal structures of Staphylococcus aureus sortase A and its substrate complex. J Biol Chem (2004) 1.60

Toll-like receptor 6 drives differentiation of tolerogenic dendritic cells and contributes to LcrV-mediated plague pathogenesis. Cell Host Microbe (2008) 1.59

Surface protein IsdC and Sortase B are required for heme-iron scavenging of Bacillus anthracis. J Bacteriol (2006) 1.59

Crystal structure of saposin B reveals a dimeric shell for lipid binding. Proc Natl Acad Sci U S A (2002) 1.58

Towards a pharmacophore for amyloid. PLoS Biol (2011) 1.58

Staphylococcus aureus IsdG and IsdI, heme-degrading enzymes with structural similarity to monooxygenases. J Biol Chem (2004) 1.58

Preventing Staphylococcus aureus sepsis through the inhibition of its agglutination in blood. PLoS Pathog (2011) 1.57

LcrV plague vaccine with altered immunomodulatory properties. Infect Immun (2005) 1.57

IsdA and IsdB antibodies protect mice against Staphylococcus aureus abscess formation and lethal challenge. Vaccine (2010) 1.57

Recurrent infections and immune evasion strategies of Staphylococcus aureus. Curr Opin Microbiol (2011) 1.54

Deconvolution and database search of complex tandem mass spectra of intact proteins: a combinatorial approach. Mol Cell Proteomics (2010) 1.53

The mitochondrial inner membrane protein mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11. FEBS Lett (2007) 1.53

A play in four acts: Staphylococcus aureus abscess formation. Trends Microbiol (2011) 1.53

Coq3 and Coq4 define a polypeptide complex in yeast mitochondria for the biosynthesis of coenzyme Q. J Biol Chem (2005) 1.53

Bacillus anthracis IsdG, a heme-degrading monooxygenase. J Bacteriol (2006) 1.51

Co-targeting of convergent nucleotide biosynthetic pathways for leukemia eradication. J Exp Med (2014) 1.49

Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Infect Immun (2006) 1.49

Targeting proteins to the cell wall of sporulating Bacillus anthracis. Mol Microbiol (2006) 1.49

Genes required for glycolipid synthesis and lipoteichoic acid anchoring in Staphylococcus aureus. J Bacteriol (2007) 1.48

Bacillus anthracis secretes proteins that mediate heme acquisition from hemoglobin. PLoS Pathog (2008) 1.47

Bacillus anthracis sortase A (SrtA) anchors LPXTG motif-containing surface proteins to the cell wall envelope. J Bacteriol (2005) 1.46