Published in Biochemistry on September 20, 2008
Multifaceted roles of alkyltransferase and related proteins in DNA repair, DNA damage, resistance to chemotherapy, and research tools. Chem Res Toxicol (2011) 1.30
The human oxidative DNA glycosylase NEIL1 excises psoralen-induced interstrand DNA cross-links in a three-stranded DNA structure. J Biol Chem (2009) 1.01
Alkyltransferase-like proteins: molecular switches between DNA repair pathways. Cell Mol Life Sci (2010) 0.92
DNA repair by reversal of DNA damage. Cold Spring Harb Perspect Biol (2013) 0.88
Repair of O4-alkylthymine by O6-alkylguanine-DNA alkyltransferases. J Biol Chem (2009) 0.86
Topologies of complexes containing O6-alkylguanine-DNA alkyltransferase and DNA. J Mol Biol (2009) 0.86
Detection and characterization of 1,2-dibromoethane-derived DNA crosslinks formed with O(6) -alkylguanine-DNA alkyltransferase. Angew Chem Int Ed Engl (2013) 0.86
DNA-protein crosslinks processed by nucleotide excision repair and homologous recombination with base and strand preference in E. coli model system. Mutat Res (2013) 0.78
Synthesis and characterization of an O(6)-2'-deoxyguanosine-alkyl-O(6)-2'-deoxyguanosine interstrand cross-link in a 5'-GNC motif and repair by human O(6)-alkylguanine-DNA alkyltransferase. Org Biomol Chem (2010) 0.76
Gut Microbiota Imbalance and Base Excision Repair Dynamics in Colon Cancer. J Cancer (2016) 0.76
Crosslinking reactions of 4-amino-6-oxo-2-vinylpyrimidine with guanine derivatives and structural analysis of the adducts. Nucleic Acids Res (2015) 0.75
What Combined Measurements From Structures and Imaging Tell Us About DNA Damage Responses. Methods Enzymol (2017) 0.75
Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr (1998) 169.28
CASTp: computed atlas of surface topography of proteins with structural and topographical mapping of functionally annotated residues. Nucleic Acids Res (2006) 9.19
Structure, function, and inhibition of O6-alkylguanine-DNA alkyltransferase. Prog Nucleic Acid Res Mol Biol (1995) 3.53
Repair of O(6)-alkylguanine by alkyltransferases. Mutat Res (2000) 3.05
DNA binding and nucleotide flipping by the human DNA repair protein AGT. Nat Struct Mol Biol (2004) 2.86
MOLE: a Voronoi diagram-based explorer of molecular channels, pores, and tunnels. Structure (2007) 2.49
Clinical relevance of MGMT in the treatment of cancer. J Clin Oncol (2002) 2.40
DNA cross-linking and monoadduct repair in nitrosourea-treated human tumour cells. Nature (1980) 1.86
Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro. Protein Expr Purif (2004) 1.78
Crystal structure of a suicidal DNA repair protein: the Ada O6-methylguanine-DNA methyltransferase from E. coli. EMBO J (1994) 1.58
O6-alkylguanine-DNA alkyltransferase: role in carcinogenesis and chemotherapy. Bioessays (2002) 1.58
Variability and regulation of O6-alkylguanine-DNA alkyltransferase. Carcinogenesis (2003) 1.54
Active and alkylated human AGT structures: a novel zinc site, inhibitor and extrahelical base binding. EMBO J (2000) 1.50
Pyridyloxobutyl adduct O6-[4-oxo-4-(3-pyridyl)butyl]guanine is present in 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone-treated DNA and is a substrate for O6-alkylguanine-DNA alkyltransferase. Chem Res Toxicol (1997) 1.38
DNA binding, nucleotide flipping, and the helix-turn-helix motif in base repair by O6-alkylguanine-DNA alkyltransferase and its implications for cancer chemotherapy. DNA Repair (Amst) (2007) 1.36
Inactivation and degradation of O(6)-alkylguanine-DNA alkyltransferase after reaction with nitric oxide. Cancer Res (2002) 1.30
The structure of the human AGT protein bound to DNA and its implications for damage detection. J Mol Biol (2005) 1.26
Crystal structure of the human O(6)-alkylguanine-DNA alkyltransferase. Nucleic Acids Res (2000) 1.25
Mechanism of inactivation of human O6-alkylguanine-DNA alkyltransferase by O6-benzylguanine. Biochemistry (1993) 1.23
A two-step nucleotide-flipping mechanism enables kinetic discrimination of DNA lesions by AGT. Proc Natl Acad Sci U S A (2008) 1.21
The chloroethylnitrosoureas: sensitivity and resistance to cancer chemotherapy at the molecular level. Cancer Invest (1997) 1.16
Kinetic analysis of steps in the repair of damaged DNA by human O6-alkylguanine-DNA alkyltransferase. J Biol Chem (2005) 1.16
Hyperthermostable protein structure maintained by intra and inter-helix ion-pairs in archaeal O6-methylguanine-DNA methyltransferase. J Mol Biol (1999) 1.12
DNA crosslinking and cytotoxicity in normal and transformed human cells treated with antitumor nitrosoureas. Proc Natl Acad Sci U S A (1980) 1.11
Characterization of a mutagenic DNA adduct formed from 1,2-dibromoethane by O6-alkylguanine-DNA alkyltransferase. J Biol Chem (2003) 1.10
Activation of bis-electrophiles to mutagenic conjugates by human O6-alkylguanine-DNA alkyltransferase. Chem Res Toxicol (2004) 1.10
Isolation of human O6-alkylguanine-DNA alkyltransferase mutants highly resistant to inactivation by O6-benzylguanine. Cancer Res (1998) 1.09
Alteration of arginine-128 to alanine abolishes the ability of human O6-alkylguanine-DNA alkyltransferase to repair methylated DNA but has no effect on its reaction with O6-benzylguanine. Biochemistry (1995) 1.09
O6-alkylguanine-DNA alkyltransferase: low pKa and high reactivity of cysteine 145. Biochemistry (2003) 1.09
Comparison of the rates of repair of O6-alkylguanines in DNA by rat liver and bacterial O6-alkylguanine-DNA alkyltransferase. Cancer Res (1984) 1.07
Cross-linking of the human DNA repair protein O6-alkylguanine DNA alkyltransferase to DNA in the presence of 1,2,3,4-diepoxybutane. Chem Res Toxicol (2006) 1.05
Cross-linking of the DNA repair protein Omicron6-alkylguanine DNA alkyltransferase to DNA in the presence of antitumor nitrogen mustards. Chem Res Toxicol (2008) 1.05
Mutagenic damage to mammalian cells by therapeutic alkylating agents. Mutat Res (1996) 1.04
Paradoxical enhancement of the toxicity of 1,2-dibromoethane by O6-alkylguanine-DNA alkyltransferase. J Biol Chem (2002) 1.03
Function of domains of human O6-alkylguanine-DNA alkyltransferase. Biochemistry (2005) 1.03
Identification of the cross-link between human O6-methylguanine-DNA methyltransferase and chloroethylnitrosourea-treated DNA. Cancer Res (1992) 1.00
Comparison of different busulfan analogues for depletion of hematopoietic stem cells and promotion of donor-type chimerism in murine bone marrow transplant recipients. Cancer Res (2000) 0.98
Differences in the rate of repair of O6-alkylguanines in different sequence contexts by O6-alkylguanine-DNA alkyltransferase. Chem Res Toxicol (2007) 0.96
Comparison of the inactivation of mammalian and bacterial O6-alkylguanine-DNA alkyltransferases by O6-benzylguanine and O6-methylguanine. Carcinogenesis (1991) 0.95
Repair of O6-propylguanine and O6-butylguanine in DNA by O6-alkylguanine-DNA alkyltransferases from rat liver and E. coli. Carcinogenesis (1985) 0.94
Differences in DNA alkylation products formed in sensitive and resistant human glioma cells treated with N-(2-chloroethyl)-N-nitrosourea. Cancer Res (1988) 0.93
Differential inactivation of mammalian and Escherichia coli O6-alkylguanine-DNA alkyltransferases by O6-benzylguanine. Biochem J (1994) 0.92
Investigation of the role of tyrosine-114 in the activity of human O6-alkylguanine-DNA alkyltranferase. Biochemistry (1998) 0.92
Production of antibodies to peptide sequences present in human O6-alkylguanine-DNA alkyltransferase and their use to detect this protein in cell extracts. Carcinogenesis (1991) 0.90
Alteration of the conserved residue tyrosine-158 to histidine renders human O6-alkylguanine-DNA alkyltransferase insensitive to the inhibitor O6-benzylguanine. Cancer Res (1999) 0.88
DNA sequence context affects repair of the tobacco-specific adduct O(6)-[4-Oxo-4-(3-pyridyl)butyl]guanine by human O(6)-alkylguanine-DNA alkyltransferases. Cancer Res (2006) 0.88
Crosslinking of DNA by busulfan. Formation of diguanyl derivatives. Biochim Biophys Acta (1980) 0.87
Specific DNA repair mechanisms may protect some human tumor cells from DNA interstrand crosslinking by chloroethylnitrosoureas but not from crosslinking by other anti-tumor alkylating agents. Carcinogenesis (1985) 0.85
O6-alkylguanine-DNA alkyltransferase has opposing effects in modulating the genotoxicity of dibromomethane and bromomethyl acetate. Chem Res Toxicol (2004) 0.84
Structural studies of MJ1529, an O6-methylguanine-DNA methyltransferase. Magn Reson Chem (2006) 0.84
O6-Benzylguanine potentiates BCNU but not busulfan toxicity in hematopoietic stem cells. Exp Hematol (2001) 0.83
Inactivation of purified human O6-alkylguanine-DNA alkyltransferase by alkylating agents or alkylated DNA. Cancer Res (1986) 0.83
Differential inactivation of polymorphic variants of human O6-alkylguanine-DNA alkyltransferase. Biochem Pharmacol (2007) 0.83
Molecular pharmacology of hepsulfam, NSC 3296801: identification of alkylated nucleosides, alkylation site, and site of DNA cross-linking. Cancer Res (1995) 0.83
Evidence that covalent complex formation between BCNU-treated oligonucleotides and E. coli alkyltransferases requires the O6-alkylguanine function. Nucleic Acids Res (1990) 0.82
Covalent capture of a human O(6)-alkylguanine alkyltransferase-DNA complex using N(1),O(6)-ethanoxanthosine, a mechanism-based crosslinker. Nucleic Acids Res (2001) 0.82
The P140K mutant of human O(6)-methylguanine-DNA-methyltransferase (MGMT) confers resistance in vitro and in vivo to temozolomide in combination with the novel MGMT inactivator O(6)-(4-bromothenyl)guanine. J Gene Med (2006) 0.82
Role of codon 160 in the sensitivity of human O6-alkylguanine-DNA alkyltransferase to O6-benzylguanine. Biochem Pharmacol (1999) 0.81
DNA-binding mechanism of the Escherichia coli Ada O(6)-alkylguanine-DNA alkyltransferase. Nucleic Acids Res (2000) 0.80
Comparison of the mechanism of action of busulfan with hepsulfam, a new antileukemic agent, in the L1210 cell line. Cancer Res (1989) 0.79
Preclinical activity of hepsulfam and busulfan in solid human tumor xenografts and human bone marrow. Anticancer Drugs (1992) 0.78
Phase I and pharmacokinetic study of hepsulfam (NSC 329680). Cancer Res (1991) 0.77
Different behavior of SCE-eliciting lesions induced by low and high doses of busulfan. Environ Mol Mutagen (2007) 0.76
Evaluation of dimethylaminosulfonates of alkane diols as a novel group of anticancer agents. Cancer Lett (2000) 0.76
Mapping the genetic architecture of gene expression in human liver. PLoS Biol (2008) 19.44
X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Q Rev Biophys (2007) 9.07
Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS). Nat Methods (2009) 6.77
Type IV pilus structure and bacterial pathogenicity. Nat Rev Microbiol (2004) 4.29
Mre11 dimers coordinate DNA end bridging and nuclease processing in double-strand-break repair. Cell (2008) 4.26
The Rad50 zinc-hook is a structure joining Mre11 complexes in DNA recombination and repair. Nature (2002) 3.48
Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions. Mol Cell (2006) 3.36
Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair. Cell (2004) 3.33
Accurate assessment of mass, models and resolution by small-angle scattering. Nature (2013) 3.18
XPD helicase structures and activities: insights into the cancer and aging phenotypes from XPD mutations. Cell (2008) 3.14
Mre11-Rad50-Nbs1 is a keystone complex connecting DNA repair machinery, double-strand break signaling, and the chromatin template. Biochem Cell Biol (2007) 3.13
Full-length archaeal Rad51 structure and mutants: mechanisms for RAD51 assembly and control by BRCA2. EMBO J (2003) 2.95
Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair. Cell (2009) 2.90
Characterizing flexible and intrinsically unstructured biological macromolecules by SAS using the Porod-Debye law. Biopolymers (2011) 2.87
DNA binding and nucleotide flipping by the human DNA repair protein AGT. Nat Struct Mol Biol (2004) 2.86
Type IV pilin structure and assembly: X-ray and EM analyses of Vibrio cholerae toxin-coregulated pilus and Pseudomonas aeruginosa PAK pilin. Mol Cell (2003) 2.78
SUMO-targeted ubiquitin ligases in genome stability. EMBO J (2007) 2.77
Microbial metalloproteomes are largely uncharacterized. Nature (2010) 2.62
Bridging the solution divide: comprehensive structural analyses of dynamic RNA, DNA, and protein assemblies by small-angle X-ray scattering. Curr Opin Struct Biol (2010) 2.45
Human flap endonuclease structures, DNA double-base flipping, and a unified understanding of the FEN1 superfamily. Cell (2011) 2.44
ATM regulates Mre11-dependent DNA end-degradation and microhomology-mediated end joining. Cell Cycle (2010) 2.32
Structural basis for isozyme-specific regulation of electron transfer in nitric-oxide synthase. J Biol Chem (2004) 2.28
Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage. EMBO J (2006) 2.27
The intricate structural chemistry of base excision repair machinery: implications for DNA damage recognition, removal, and repair. DNA Repair (Amst) (2007) 2.26
A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA. Mol Cell (2006) 2.23
DNA base damage recognition and removal: new twists and grooves. Mutat Res (2005) 2.21
The DNA repair endonuclease XPG interacts directly and functionally with the WRN helicase defective in Werner syndrome. Cell Cycle (2011) 2.15
Conserved XPB core structure and motifs for DNA unwinding: implications for pathway selection of transcription or excision repair. Mol Cell (2006) 2.13
Identification of a new cryptochrome class. Structure, function, and evolution. Mol Cell (2003) 2.11
Elucidating mechanisms of drug-induced toxicity. Nat Rev Drug Discov (2005) 2.06
Systematic genetic and genomic analysis of cytochrome P450 enzyme activities in human liver. Genome Res (2010) 2.06
A SIM-ultaneous role for SUMO and ubiquitin. Trends Biochem Sci (2008) 2.05
DNA adduct bypass polymerization by Sulfolobus solfataricus DNA polymerase Dpo4: analysis and crystal structures of multiple base pair substitution and frameshift products with the adduct 1,N2-ethenoguanine. J Biol Chem (2005) 2.02
Mre11-Rad50-Nbs1 conformations and the control of sensing, signaling, and effector responses at DNA double-strand breaks. DNA Repair (Amst) (2010) 1.96
Rad50/SMC proteins and ABC transporters: unifying concepts from high-resolution structures. Curr Opin Struct Biol (2003) 1.93
Ku and DNA-dependent protein kinase dynamic conformations and assembly regulate DNA binding and the initial non-homologous end joining complex. J Biol Chem (2009) 1.91
MDB: the Metalloprotein Database and Browser at The Scripps Research Institute. Nucleic Acids Res (2002) 1.89
Recognition of RNA polymerase II and transcription bubbles by XPG, CSB, and TFIIH: insights for transcription-coupled repair and Cockayne Syndrome. Mol Cell (2005) 1.89
Accurate SAXS profile computation and its assessment by contrast variation experiments. Biophys J (2013) 1.83
ALS mutants of human superoxide dismutase form fibrous aggregates via framework destabilization. J Mol Biol (2003) 1.82
Polyamine catabolism and disease. Biochem J (2009) 1.79
Hexameric structures of the archaeal secretion ATPase GspE and implications for a universal secretion mechanism. EMBO J (2007) 1.77
Complex reactions catalyzed by cytochrome P450 enzymes. Biochim Biophys Acta (2006) 1.76
Efficient and high fidelity incorporation of dCTP opposite 7,8-dihydro-8-oxodeoxyguanosine by Sulfolobus solfataricus DNA polymerase Dpo4. J Biol Chem (2005) 1.74
DNA charge transport as a first step in coordinating the detection of lesions by repair proteins. Proc Natl Acad Sci U S A (2012) 1.71
A structural model for regulation of NHEJ by DNA-PKcs autophosphorylation. DNA Repair (Amst) (2010) 1.71
Flipping of alkylated DNA damage bridges base and nucleotide excision repair. Nature (2009) 1.70
Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source. J Appl Crystallogr (2013) 1.69
DNA double-strand break repair from head to tail. Curr Opin Struct Biol (2002) 1.67
ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair. Nat Struct Mol Biol (2011) 1.66
Nickel superoxide dismutase structure and mechanism. Biochemistry (2004) 1.66
Phase I trial of temozolomide plus O6-benzylguanine for patients with recurrent or progressive malignant glioma. J Clin Oncol (2005) 1.63
Kinetics and thermodynamics of ligand binding by cytochrome P450 3A4. J Biol Chem (2006) 1.63
The crystal structure of spermidine synthase with a multisubstrate adduct inhibitor. Nat Struct Biol (2002) 1.63
Translesion synthesis across bulky N2-alkyl guanine DNA adducts by human DNA polymerase kappa. J Biol Chem (2006) 1.62
Improving small-angle X-ray scattering data for structural analyses of the RNA world. RNA (2010) 1.60
WRN exonuclease structure and molecular mechanism imply an editing role in DNA end processing. Nat Struct Mol Biol (2006) 1.55
Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of Streptomyces coelicolor A3(2) cytochrome P450 158A2. J Biol Chem (2005) 1.52
Phase II trial of carmustine plus O(6)-benzylguanine for patients with nitrosourea-resistant recurrent or progressive malignant glioma. J Clin Oncol (2002) 1.50
XRCC4 protein interactions with XRCC4-like factor (XLF) create an extended grooved scaffold for DNA ligation and double strand break repair. J Biol Chem (2011) 1.48
Roles of NADPH-P450 reductase and apo- and holo-cytochrome b5 on xenobiotic oxidations catalyzed by 12 recombinant human cytochrome P450s expressed in membranes of Escherichia coli. Protein Expr Purif (2002) 1.46
Protein mimicry of DNA and pathway regulation. DNA Repair (Amst) (2005) 1.46
Cytochrome P450 activation of arylamines and heterocyclic amines. Annu Rev Pharmacol Toxicol (2005) 1.46
DNA apurinic-apyrimidinic site binding and excision by endonuclease IV. Nat Struct Mol Biol (2008) 1.46
Translesion synthesis across O6-alkylguanine DNA adducts by recombinant human DNA polymerases. J Biol Chem (2006) 1.45
X-linked spermine synthase gene (SMS) defect: the first polyamine deficiency syndrome. Eur J Hum Genet (2003) 1.45
XPB and XPD helicases in TFIIH orchestrate DNA duplex opening and damage verification to coordinate repair with transcription and cell cycle via CAK kinase. DNA Repair (Amst) (2011) 1.45
Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures. Proc Natl Acad Sci U S A (2003) 1.41
Report of the wwPDB Small-Angle Scattering Task Force: data requirements for biomolecular modeling and the PDB. Structure (2013) 1.39
Crystal structure of human spermine synthase: implications of substrate binding and catalytic mechanism. J Biol Chem (2008) 1.39
Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4. J Biol Chem (2007) 1.38
Proliferating cell nuclear antigen loaded onto double-stranded DNA: dynamics, minor groove interactions and functional implications. Nucleic Acids Res (2006) 1.37
Structural basis for endothelial nitric oxide synthase binding to calmodulin. EMBO J (2003) 1.37
Functional motifs in the (6-4) photolyase crystal structure make a comparative framework for DNA repair photolyases and clock cryptochromes. Proc Natl Acad Sci U S A (2009) 1.36
Super-resolution in solution X-ray scattering and its applications to structural systems biology. Annu Rev Biophys (2013) 1.36
DNA binding, nucleotide flipping, and the helix-turn-helix motif in base repair by O6-alkylguanine-DNA alkyltransferase and its implications for cancer chemotherapy. DNA Repair (Amst) (2007) 1.36
DNA-binding mechanism of O6-alkylguanine-DNA alkyltransferase. Effects of protein and DNA alkylation on complex stability. J Biol Chem (2002) 1.34
Interstrand cross-links generated by abasic sites in duplex DNA. J Am Chem Soc (2007) 1.34
XLF regulates filament architecture of the XRCC4·ligase IV complex. Structure (2010) 1.33
Human DNA ligase III recognizes DNA ends by dynamic switching between two DNA-bound states. Biochemistry (2010) 1.32
Superoxide dismutase from the eukaryotic thermophile Alvinella pompejana: structures, stability, mechanism, and insights into amyotrophic lateral sclerosis. J Mol Biol (2008) 1.32
Structural dynamics in DNA damage signaling and repair. Curr Opin Struct Biol (2010) 1.31
Structural and functional elucidation of the mechanism promoting error-prone synthesis by human DNA polymerase kappa opposite the 7,8-dihydro-8-oxo-2'-deoxyguanosine adduct. J Biol Chem (2009) 1.31
Inactivation and degradation of O(6)-alkylguanine-DNA alkyltransferase after reaction with nitric oxide. Cancer Res (2002) 1.30
Cloning, expression, and characterization of a nitric oxide synthase protein from Deinococcus radiodurans. Proc Natl Acad Sci U S A (2001) 1.30
Comprehensive macromolecular conformations mapped by quantitative SAXS analyses. Nat Methods (2013) 1.29
Degradation of the alkylated form of the DNA repair protein, O(6)-alkylguanine-DNA alkyltransferase. Carcinogenesis (2002) 1.29
Analysis of coumarin 7-hydroxylation activity of cytochrome P450 2A6 using random mutagenesis. J Biol Chem (2005) 1.29
gammaH2A binds Brc1 to maintain genome integrity during S-phase. EMBO J (2010) 1.28
Structural characterization of zinc-deficient human superoxide dismutase and implications for ALS. J Mol Biol (2007) 1.26
Structures of endonuclease V with DNA reveal initiation of deaminated adenine repair. Nat Struct Mol Biol (2009) 1.26
Adduct size limits efficient and error-free bypass across bulky N2-guanine DNA lesions by human DNA polymerase eta. J Mol Biol (2005) 1.26
A novel processive mechanism for DNA synthesis revealed by structure, modeling and mutagenesis of the accessory subunit of human mitochondrial DNA polymerase. J Mol Biol (2006) 1.25
Anchored plasticity opens doors for selective inhibitor design in nitric oxide synthase. Nat Chem Biol (2008) 1.25
Recombinant enzymes overexpressed in bacteria show broad catalytic specificity of human cytochrome P450 2W1 and limited activity of human cytochrome P450 2S1. Mol Pharmacol (2006) 1.24
Sulfolobus solfataricus DNA polymerase Dpo4 is partially inhibited by "wobble" pairing between O6-methylguanine and cytosine, but accurate bypass is preferred. J Biol Chem (2006) 1.22