Live cell imaging of phosphoinositides with expressed inositide binding protein domains.

PubWeight™: 1.11‹?› | Rank: Top 10%

🔗 View Article (PMC 2644460)

Published in Methods on October 16, 2008

Authors

Péter Várnai1, Tamas Balla

Author Affiliations

1: Department of Physiology, Semmelweis University Faculty of Medicine, Budapest, H-1088 Budapest, Puskin utca 9, Hungary, Bethesda, MD 20892, USA. varnai@puskin.sote.hu

Articles citing this

Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling. J Cell Biol (2010) 2.65

Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P(2). Biochem J (2009) 2.00

PI3K signalling in B- and T-lymphocytes: new developments and therapeutic advances. Biochem J (2012) 1.56

Palmitoylation targets AKAP79 protein to lipid rafts and promotes its regulation of calcium-sensitive adenylyl cyclase type 8. J Biol Chem (2011) 1.00

Actin and endocytosis in budding yeast. Genetics (2015) 0.99

Phosphoinositide 3-kinase regulates plasma membrane targeting of the Ras-specific exchange factor RasGRP1. J Biol Chem (2011) 0.96

Unbalancing the phosphatidylinositol-4,5-bisphosphate-cofilin interaction impairs cell steering. Mol Biol Cell (2009) 0.94

Regulation of Golgi function via phosphoinositide lipids. Semin Cell Dev Biol (2009) 0.92

A plasmid-based multigene expression system for mammalian cells. Nat Commun (2010) 0.92

Effect of protein S-glutathionylation on Ca2+ homeostasis in cultured aortic endothelial cells. Am J Physiol Heart Circ Physiol (2010) 0.92

Green light to illuminate signal transduction events. Trends Cell Biol (2009) 0.89

Signaling at the Golgi. Cold Spring Harb Perspect Biol (2011) 0.88

Challenges in studying phospholipid signaling. Nat Chem Biol (2010) 0.82

Sphingolipid homeostasis in the endoplasmic reticulum and beyond. Cold Spring Harb Perspect Biol (2013) 0.82

A method to control phosphoinositides and to analyze PTEN function in living cells using voltage sensitive phosphatases. Front Pharmacol (2015) 0.82

BRET-monitoring of the dynamic changes of inositol lipid pools in living cells reveals a PKC-dependent PtdIns4P increase upon EGF and M3 receptor activation. Biochim Biophys Acta (2015) 0.79

PtdIns4KIIα generates endosomal PtdIns(4)P and is required for receptor sorting at early endosomes. Mol Biol Cell (2016) 0.79

Actin-based confinement of calcium responses during Shigella invasion. Nat Commun (2013) 0.79

A novel phosphatidylinositol 4,5-bisphosphate binding domain mediates plasma membrane localization of ExoU and other patatin-like phospholipases. J Biol Chem (2014) 0.78

A novel imaging method revealed phosphatidylinositol 3,5-bisphosphate-rich domains in the endosome/lysosome membrane. Commun Integr Biol (2016) 0.75

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes. J Vis Exp (2016) 0.75

Articles cited by this

The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature (1984) 28.58

A guide to choosing fluorescent proteins. Nat Methods (2005) 18.55

Inositol trisphosphate and diacylglycerol as second messengers. Biochem J (1984) 14.37

The fluorescent toolbox for assessing protein location and function. Science (2006) 14.02

Direct regulation of the Akt proto-oncogene product by phosphatidylinositol-3,4-bisphosphate. Science (1997) 8.81

Phosphoinositide kinases. Annu Rev Biochem (1998) 8.51

EEA1 links PI(3)K function to Rab5 regulation of endosome fusion. Nature (1998) 7.92

Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells. EMBO J (2000) 7.48

Membrane recognition by phospholipid-binding domains. Nat Rev Mol Cell Biol (2008) 7.43

Signalling through the lipid products of phosphoinositide-3-OH kinase. Nature (1997) 7.20

Polarization of chemoattractant receptor signaling during neutrophil chemotaxis. Science (2000) 6.93

Visualization of phosphoinositides that bind pleckstrin homology domains: calcium- and agonist-induced dynamic changes and relationship to myo-[3H]inositol-labeled phosphoinositide pools. J Cell Biol (1998) 6.70

Regulation of phosphoinositide-specific phospholipase C. Annu Rev Biochem (2001) 6.42

Receptor-induced transient reduction in plasma membrane PtdIns(4,5)P2 concentration monitored in living cells. Curr Biol (1998) 5.94

Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate. Nature (1988) 5.88

Rapid chemically induced changes of PtdIns(4,5)P2 gate KCNQ ion channels. Science (2006) 5.10

FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P. Nat Cell Biol (2004) 4.85

The PX domains of p47phox and p40phox bind to lipid products of PI(3)K. Nat Cell Biol (2001) 4.64

Specific and high-affinity binding of inositol phosphates to an isolated pleckstrin homology domain. Proc Natl Acad Sci U S A (1995) 4.10

Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. Mol Cell (1998) 4.01

Signaling by phosphoinositide-3,4,5-trisphosphate through proteins containing pleckstrin and Sec7 homology domains. Science (1997) 3.91

G-protein signaling through tubby proteins. Science (2001) 3.59

Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells. J Cell Biol (2006) 3.56

Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities. Biochem J (2000) 3.53

Distinct specificity in the recognition of phosphoinositides by the pleckstrin homology domains of dynamin and Bruton's tyrosine kinase. EMBO J (1996) 3.41

The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor. Cell (2003) 3.36

Targeting of Golgi-specific pleckstrin homology domains involves both PtdIns 4-kinase-dependent and -independent components. Curr Biol (2002) 3.23

A comparative analysis of the phosphoinositide binding specificity of pleckstrin homology domains. J Biol Chem (1997) 3.19

Specificity and promiscuity in phosphoinositide binding by pleckstrin homology domains. J Biol Chem (1998) 3.07

Phosphatidylinositol 4-kinases: old enzymes with emerging functions. Trends Cell Biol (2006) 3.06

Akt/PKB localisation and 3' phosphoinositide generation at sites of epithelial cell-matrix and cell-cell interaction. Curr Biol (1999) 3.00

Monitoring agonist-induced phospholipase C activation in live cells by fluorescence resonance energy transfer. J Biol Chem (2001) 2.88

Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains. Mol Cell (2004) 2.83

Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors. EMBO J (2004) 2.73

Receptor-mediated regulation of PI3Ks confines PI(3,4,5)P3 to the leading edge of chemotaxing cells. Mol Biol Cell (2003) 2.56

Correcting confocal acquisition to optimize imaging of fluorescence resonance energy transfer by sensitized emission. Biophys J (2004) 2.55

Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations. J Cell Biol (2003) 2.55

Phosphatidylinositol 3-kinase-dependent membrane association of the Bruton's tyrosine kinase pleckstrin homology domain visualized in single living cells. J Biol Chem (1999) 2.43

Phosphatidylinositol phosphate kinases put PI4,5P(2) in its place. J Membr Biol (2003) 2.43

A plasma membrane pool of phosphatidylinositol 4-phosphate is generated by phosphatidylinositol 4-kinase type-III alpha: studies with the PH domains of the oxysterol binding protein and FAPP1. Mol Biol Cell (2005) 2.39

PtdIns(3)P regulates the neutrophil oxidase complex by binding to the PX domain of p40(phox). Nat Cell Biol (2001) 2.39

Phosphatidylinositol 3,5-bisphosphate: metabolism and cellular functions. Trends Biochem Sci (2005) 2.20

Insulin-dependent translocation of ARNO to the plasma membrane of adipocytes requires phosphatidylinositol 3-kinase. Curr Biol (1998) 2.08

The pleckstrin homology domain of oxysterol-binding protein recognises a determinant specific to Golgi membranes. Curr Biol (1998) 1.98

Quantitative multiphoton spectral imaging and its use for measuring resonance energy transfer. Biophys J (2005) 1.98

The phosphoinositide (PI) 3-kinase family. J Cell Sci (2003) 1.94

Multiple pools of phosphatidylinositol 4-phosphate detected using the pleckstrin homology domain of Osh2p. J Biol Chem (2004) 1.88

Structural insights into the regulation of PDK1 by phosphoinositides and inositol phosphates. EMBO J (2004) 1.77

PtdIns(4,5)P2 functions at the cleavage furrow during cytokinesis. Curr Biol (2005) 1.73

Determining selectivity of phosphoinositide-binding domains. Methods (2006) 1.62

Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130. J Biol Chem (2002) 1.61

The role of phosphatases in inositol signaling reactions. J Biol Chem (1999) 1.54

How accurately can we image inositol lipids in living cells? Trends Pharmacol Sci (2000) 1.47

PtdIns5P activates the host cell PI3-kinase/Akt pathway during Shigella flexneri infection. EMBO J (2006) 1.45

Distinct polyphosphoinositide binding selectivities for pleckstrin homology domains of GRP1-like proteins based on diglycine versus triglycine motifs. J Biol Chem (2000) 1.45

Production of PtdInsP3 at endomembranes is triggered by receptor endocytosis. Nat Cell Biol (2003) 1.42

Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body. Dev Cell (2003) 1.37

Nerve growth factor- and epidermal growth factor-stimulated translocation of the ADP-ribosylation factor-exchange factor GRP1 to the plasma membrane of PC12 cells requires activation of phosphatidylinositol 3-kinase and the GRP1 pleckstrin homology domain. Biochem J (1998) 1.36

Signal propagation from membrane messengers to nuclear effectors revealed by reporters of phosphoinositide dynamics and Akt activity. Proc Natl Acad Sci U S A (2005) 1.34

Cytosolic inositol 1,4,5-trisphosphate dynamics during intracellular calcium oscillations in living cells. J Cell Biol (2006) 1.33

Fluorescent biosensor for quantitative real-time measurements of inositol 1,4,5-trisphosphate in single living cells. J Biol Chem (2004) 1.22

Visualizing PI3 kinase-mediated cell-cell signaling during Dictyostelium development. Curr Biol (2002) 1.21

The PH domain and the polybasic c domain of cytohesin-1 cooperate specifically in plasma membrane association and cellular function. Mol Biol Cell (1998) 1.20

Elimination of plasma membrane phosphatidylinositol (4,5)-bisphosphate is required for exocytosis from mast cells. J Cell Sci (2006) 1.10

Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo. Biochem J (2002) 1.08

PTEN and myotubularins: families of phosphoinositide phosphatases. Methods Enzymol (2003) 1.07

Regulation of phosphoinositide signaling by the inositol polyphosphate 5-phosphatases. IUBMB Life (2006) 1.07

Single-cell imaging of graded Ins(1,4,5)P3 production following G-protein-coupled-receptor activation. Biochem J (2001) 1.02

EGF-and NGF-stimulated translocation of cytohesin-1 to the plasma membrane of PC12 cells requires PI 3-kinase activation and a functional cytohesin-1 PH domain. J Cell Sci (1999) 1.00

Generation of phosphatidylinositol-specific antibodies and their characterization. Biochem Soc Trans (1999) 0.92

Single cell imaging of PI3K activity and glucose transporter insertion into the plasma membrane by dual color evanescent wave microscopy. Sci STKE (2003) 0.90

Phosphoinositide-containing polymerized liposomes: stable membrane-mimetic vesicles for protein-lipid binding analysis. Bioconjug Chem (2005) 0.87

The pleckstrin homology domain of phosphoinositide-specific phospholipase Cdelta4 is not a critical determinant of the membrane localization of the enzyme. J Biol Chem (2004) 0.86

Articles by these authors

A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling. Cell (2006) 10.06

Viral reorganization of the secretory pathway generates distinct organelles for RNA replication. Cell (2010) 4.47

Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells. J Cell Biol (2006) 3.56

Control of cell polarity and motility by the PtdIns(3,4,5)P3 phosphatase SHIP1. Nat Cell Biol (2006) 3.19

Phosphatidylinositol 4-kinases: old enzymes with emerging functions. Trends Cell Biol (2006) 3.06

Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment. Cell Host Microbe (2011) 3.05

PI4P and PI(4,5)P2 are essential but independent lipid determinants of membrane identity. Science (2012) 3.05

Visualization and manipulation of plasma membrane-endoplasmic reticulum contact sites indicates the presence of additional molecular components within the STIM1-Orai1 Complex. J Biol Chem (2007) 3.02

Loss of endocytic clathrin-coated pits upon acute depletion of phosphatidylinositol 4,5-bisphosphate. Proc Natl Acad Sci U S A (2007) 2.52

Dual regulation of TRPV1 by phosphoinositides. J Neurosci (2007) 2.48

A plasma membrane pool of phosphatidylinositol 4-phosphate is generated by phosphatidylinositol 4-kinase type-III alpha: studies with the PH domains of the oxysterol binding protein and FAPP1. Mol Biol Cell (2005) 2.39

Active Arf6 recruits ARNO/cytohesin GEFs to the PM by binding their PH domains. Mol Biol Cell (2007) 2.15

Activation of STIM1-Orai1 involves an intramolecular switching mechanism. Sci Signal (2010) 1.89

Selective cellular effects of overexpressed pleckstrin-homology domains that recognize PtdIns(3,4,5)P3 suggest their interaction with protein binding partners. J Cell Sci (2005) 1.80

Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments. J Biol Chem (2002) 1.78

Maintenance of hormone-sensitive phosphoinositide pools in the plasma membrane requires phosphatidylinositol 4-kinase IIIalpha. Mol Biol Cell (2007) 1.77

Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130. J Biol Chem (2002) 1.61

Live cell imaging of phosphoinositide dynamics with fluorescent protein domains. Biochim Biophys Acta (2006) 1.53

Phosphatidylinositol 4-kinase IIIbeta regulates the transport of ceramide between the endoplasmic reticulum and Golgi. J Biol Chem (2006) 1.52

c-Met must translocate to the nucleus to initiate calcium signals. J Biol Chem (2007) 1.52

A highly dynamic ER-derived phosphatidylinositol-synthesizing organelle supplies phosphoinositides to cellular membranes. Dev Cell (2011) 1.50

Differential PI 3-kinase dependence of early and late phases of recycling of the internalized AT1 angiotensin receptor. J Cell Biol (2002) 1.43

Acute manipulation of Golgi phosphoinositides to assess their importance in cellular trafficking and signaling. Proc Natl Acad Sci U S A (2010) 1.42

Dependence of STIM1/Orai1-mediated calcium entry on plasma membrane phosphoinositides. J Biol Chem (2009) 1.36

A membrane capture assay for lipid kinase activity. Nat Protoc (2007) 1.32

Structural determinants of Ras-Raf interaction analyzed in live cells. Mol Biol Cell (2002) 1.31

Live cell imaging with protein domains capable of recognizing phosphatidylinositol 4,5-bisphosphate; a comparative study. BMC Cell Biol (2009) 1.31

A novel probe for phosphatidylinositol 4-phosphate reveals multiple pools beyond the Golgi. J Cell Biol (2014) 1.31

Phosphatidylinositol 4-kinases: hostages harnessed to build panviral replication platforms. Trends Biochem Sci (2012) 1.29

Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate. J Cell Biol (2007) 1.25

Targeted expression of the inositol 1,4,5-triphosphate receptor (IP3R) ligand-binding domain releases Ca2+ via endogenous IP3R channels. Proc Natl Acad Sci U S A (2005) 1.24

Dual roles for the Drosophila PI 4-kinase four wheel drive in localizing Rab11 during cytokinesis. J Cell Biol (2009) 1.23

STIM and Orai: the long-awaited constituents of store-operated calcium entry. Trends Pharmacol Sci (2009) 1.20

Pharmacological and genetic targeting of the PI4KA enzyme reveals its important role in maintaining plasma membrane phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate levels. J Biol Chem (2014) 1.15

Visualization and manipulation of phosphoinositide dynamics in live cells using engineered protein domains. Pflugers Arch (2007) 1.14

G protein-coupled receptor-promoted trafficking of Gbeta1gamma2 leads to AKT activation at endosomes via a mechanism mediated by Gbeta1gamma2-Rab11a interaction. Mol Biol Cell (2008) 1.08

Design of drug-resistant alleles of type-III phosphatidylinositol 4-kinases using mutagenesis and molecular modeling. Biochemistry (2008) 1.06

Enteropathogenic Escherichia coli subverts phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate upon epithelial cell infection. Mol Biol Cell (2008) 1.03

Intracellular curvature-generating proteins in cell-to-cell fusion. Biochem J (2011) 1.02

Two phosphatidylinositol 4-kinases control lysosomal delivery of the Gaucher disease enzyme, β-glucocerebrosidase. Mol Biol Cell (2012) 1.00

The dynamics of plasma membrane PtdIns(4,5)P(2) at fertilization of mouse eggs. J Cell Sci (2002) 0.99

Acute depletion of plasma membrane phosphatidylinositol 4,5-bisphosphate impairs specific steps in endocytosis of the G-protein-coupled receptor. J Cell Sci (2012) 0.95

A homogeneous and nonisotopic assay for phosphatidylinositol 4-kinases. Anal Biochem (2011) 0.94

A PH domain in the Arf GTPase-activating protein (GAP) ARAP1 binds phosphatidylinositol 3,4,5-trisphosphate and regulates Arf GAP activity independently of recruitment to the plasma membranes. J Biol Chem (2009) 0.92

Inositol lipid regulation of lipid transfer in specialized membrane domains. Trends Cell Biol (2013) 0.90

Store-operated Ca2+ influx and subplasmalemmal mitochondria. Cell Calcium (2009) 0.90

Crucial role of phosphatidylinositol 4-kinase IIIalpha in development of zebrafish pectoral fin is linked to phosphoinositide 3-kinase and FGF signaling. J Cell Sci (2009) 0.89

Recruitment of arfaptins to the trans-Golgi network by PI(4)P and their involvement in cargo export. EMBO J (2013) 0.87

Phosphoinositide 3-kinase is required for intracellular Listeria monocytogenes actin-based motility and filopod formation. J Biol Chem (2005) 0.86

Secretion of VEGF-165 has unique characteristics, including shedding from the plasma membrane. Mol Biol Cell (2014) 0.86

The pleckstrin homology domain of phosphoinositide-specific phospholipase Cdelta4 is not a critical determinant of the membrane localization of the enzyme. J Biol Chem (2004) 0.86

Distinct properties of the two isoforms of CDP-diacylglycerol synthase. Biochemistry (2014) 0.80

Genetic and functional studies of phosphatidyl-inositol 4-kinase type IIIα. Biochim Biophys Acta (2011) 0.79

Demonstration of angiotensin II-induced Ras activation in the trans-Golgi network and endoplasmic reticulum using bioluminescence resonance energy transfer-based biosensors. J Biol Chem (2010) 0.79

βIII spectrin regulates the structural integrity and the secretory protein transport of the Golgi complex. J Biol Chem (2012) 0.78