Published in Anal Biochem on December 28, 2009
Imaging proteins inside cells with fluorescent tags. Trends Biotechnol (2011) 1.17
Structural characterization of full-length NSF and 20S particles. Nat Struct Mol Biol (2012) 1.08
Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF). Biochim Biophys Acta (2011) 1.08
Degradable conjugates from oxanorbornadiene reagents. J Am Chem Soc (2012) 1.03
Structural basis for activation of calcineurin by calmodulin. J Mol Biol (2011) 0.92
Nucleotide-dependent conformational changes in the N-Ethylmaleimide Sensitive Factor (NSF) and their potential role in SNARE complex disassembly. J Struct Biol (2012) 0.89
Sets of RNA repeated tags and hybridization-sensitive fluorescent probes for distinct images of RNA in a living cell. PLoS One (2010) 0.82
Histidine-tag-directed chromophores for tracer analyses in the analytical ultracentrifuge. Methods (2010) 0.77
Direct observation of dynamic mechanical regulation of DNA condensation by environmental stimuli. Angew Chem Int Ed Engl (2014) 0.76
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem (1976) 903.81
Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling. Biophys J (2000) 21.93
New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J Chromatogr (1987) 6.17
Resonance energy transfer: methods and applications. Anal Biochem (1994) 4.99
OligoCalc: an online oligonucleotide properties calculator. Nucleic Acids Res (2007) 4.96
Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport. Proc Natl Acad Sci U S A (1988) 4.63
N-ethylmaleimide-sensitive fusion protein: a trimeric ATPase whose hydrolysis of ATP is required for membrane fusion. J Cell Biol (1994) 3.59
Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proc Natl Acad Sci U S A (1991) 3.57
DNA binding and nucleotide flipping by the human DNA repair protein AGT. Nat Struct Mol Biol (2004) 2.86
Calculating sedimentation coefficient distributions by direct modeling of sedimentation velocity concentration profiles. Methods Enzymol (2004) 2.24
Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni(2+):nitrilotriacetic Acid (n)-fluorochrome conjugates. J Am Chem Soc (2001) 2.08
High-affinity adaptors for switchable recognition of histidine-tagged proteins. J Am Chem Soc (2005) 2.06
Mapping the structure and conformational movements of proteins with transition metal ion FRET. Nat Methods (2009) 1.92
Affinity purification of histidine-tagged proteins. Mol Biol Rep (1993) 1.57
Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation. J Am Chem Soc (2006) 1.53
Cellular functions of NSF: not just SNAPs and SNAREs. FEBS Lett (2007) 1.42
DNA-binding mechanism of O6-alkylguanine-DNA alkyltransferase. Effects of protein and DNA alkylation on complex stability. J Biol Chem (2002) 1.34
Crystal structure of the amino-terminal domain of N-ethylmaleimide-sensitive fusion protein. Nat Cell Biol (1999) 1.24
Nucleic acid conformational changes essential for HIV-1 nucleocapsid protein-mediated inhibition of self-priming in minus-strand transfer. J Mol Biol (2003) 1.22
Facile synthesis of multivalent nitrilotriacetic acid (NTA) and NTA conjugates for analytical and drug delivery applications. Bioconjug Chem (2006) 1.19
Interactions of human O6-alkylguanine-DNA alkyltransferase (AGT) with short single-stranded DNAs. J Biol Chem (2006) 1.19
Ni-NTA-gold clusters target His-tagged proteins. J Struct Biol (1999) 1.16
A revised model for the oligomeric state of the N-ethylmaleimide-sensitive fusion protein, NSF. J Biol Chem (1998) 1.11
Applications of fluorescence anisotropy to the study of protein-DNA interactions. Methods Cell Biol (2008) 1.10
High-affinity chelator thiols for switchable and oriented immobilization of histidine-tagged proteins: a generic platform for protein chip technologies. Chemistry (2005) 1.10
Dissecting the N-ethylmaleimide-sensitive factor: required elements of the N and D1 domains. J Biol Chem (2009) 1.00
A nickel chelate microtiter plate assay for six histidine-containing proteins. Anal Biochem (1996) 1.00
Noncovalent, site-specific biotinylation of histidine-tagged proteins. Anal Chem (2007) 0.96
Preparation and characterization of nickel nanoparticles for binding to his-tag proteins and antigens. Pharm Res (2006) 0.94
One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin. Mol Biotechnol (1995) 0.94
Attaching histidine-tagged peptides and proteins to lipid-based carriers through use of metal-ion-chelating lipids. Biochim Biophys Acta (2002) 0.92
Mutations in the Ada O6-alkylguanine-DNA alkyltransferase conferring sensitivity to inactivation by O6-benzylguanine and 2,4-diamino-6-benzyloxy-5-nitrosopyrimidine. Carcinogenesis (1995) 0.92
Interactions of human O(6)-alkylguanine-DNA alkyltransferase (AGT) with short double-stranded DNAs. Biochemistry (2008) 0.90
Immobilization of His-tagged proteins on nickel-chelating nanolipoprotein particles. Bioconjug Chem (2009) 0.88
Dual-mode fluorophore-doped nickel nitrilotriacetic acid-modified silica nanoparticles combine histidine-tagged protein purification with site-specific fluorophore labeling. J Am Chem Soc (2007) 0.88
Single-step formation of a biorecognition layer for assaying histidine-tagged proteins. Anal Chem (2004) 0.87
A comparative study of the biochemical properties of human and mouse recombinant O6-methylguanine-DNA methyltransferases. Carcinogenesis (1995) 0.86
A facile method for reversibly linking a recombinant protein to DNA. Chembiochem (2009) 0.85
Use of difference boundary sedimentation velocity to investigate nonspecific protein-nucleic acid interactions. Biochemistry (1980) 0.85
Ni-nitrilotriacetic acid-modified quantum dots as a site-specific labeling agent of histidine-tagged proteins in live cells. Chem Commun (Camb) (2008) 0.80
Conjugation of DNA with protein using His-tag chemistry and its application to the aptamer-based detection system. Biotechnol Lett (2008) 0.79
Synthesis of the chelator lipid nitrilotriacetic acid ditetradecylamine (NTA-DTDA) and its use with the IAsys biosensor to study receptor-ligand interactions on model membranes. Biochim Biophys Acta (2001) 0.77
Use of alpha-N,N-bis[carboxymethyl]lysine-modified peroxidase in immunoassays. Anal Biochem (1995) 0.77
AAA+ proteins: have engine, will work. Nat Rev Mol Cell Biol (2005) 7.73
Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid interactions. Nat Protoc (2007) 3.31
Conserved arginine residues implicated in ATP hydrolysis, nucleotide-sensing, and inter-subunit interactions in AAA and AAA+ ATPases. J Struct Biol (2004) 2.15
Endobrevin/VAMP-8 is the primary v-SNARE for the platelet release reaction. Mol Biol Cell (2006) 2.02
Endobrevin/VAMP-8-dependent dense granule release mediates thrombus formation in vivo. Blood (2009) 2.00
Munc13-4 is a limiting factor in the pathway required for platelet granule release and hemostasis. Blood (2010) 2.00
A family with tau-negative frontotemporal dementia and neuronal intranuclear inclusions linked to chromosome 17. Brain (2006) 1.75
Flipping of alkylated DNA damage bridges base and nucleotide excision repair. Nature (2009) 1.70
A novel role for platelet secretion in angiogenesis: mediating bone marrow-derived cell mobilization and homing. Blood (2011) 1.61
Cellular functions of NSF: not just SNAPs and SNAREs. FEBS Lett (2007) 1.42
PKCalpha regulates platelet granule secretion and thrombus formation in mice. J Clin Invest (2009) 1.41
Impact of diabetes and smoking on mortality in tuberculosis. PLoS One (2013) 1.36
Syntaxin-11, but not syntaxin-2 or syntaxin-4, is required for platelet secretion. Blood (2012) 1.35
DNA-binding mechanism of O6-alkylguanine-DNA alkyltransferase. Effects of protein and DNA alkylation on complex stability. J Biol Chem (2002) 1.34
Munc18b/STXBP2 is required for platelet secretion. Blood (2012) 1.30
Type I PDZ ligands are sufficient to promote rapid recycling of G Protein-coupled receptors independent of binding to N-ethylmaleimide-sensitive factor. J Biol Chem (2004) 1.27
Phosphorylation of SNAP-23 regulates exocytosis from mast cells. J Biol Chem (2004) 1.23
Interactions of human O6-alkylguanine-DNA alkyltransferase (AGT) with short single-stranded DNAs. J Biol Chem (2006) 1.19
Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release. Blood (2003) 1.18
ATP effects on insulin-degrading enzyme are mediated primarily through its triphosphate moiety. J Biol Chem (2004) 1.15
Furin processing of semaphorin 3F determines its anti-angiogenic activity by regulating direct binding and competition for neuropilin. Biochemistry (2010) 1.14
Arf6 plays an early role in platelet activation by collagen and convulxin. Blood (2005) 1.12
The platelet release reaction: just when you thought platelet secretion was simple. Curr Opin Hematol (2008) 1.10
Regulation, mechanisms and proposed function of ferritin translocation to cell nuclei. J Cell Sci (2002) 1.10
Voltage gated carbon nanotube membranes. Langmuir (2007) 1.08
Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF). Biochim Biophys Acta (2011) 1.08
Platelet secretion is kinetically heterogeneous in an agonist-responsive manner. Blood (2012) 1.06
Platelets protect from septic shock by inhibiting macrophage-dependent inflammation via the cyclooxygenase 1 signalling pathway. Nat Commun (2013) 1.05
Structural basis of natural promoter recognition by a unique nuclear receptor, HNF4alpha. Diabetes gene product. J Biol Chem (2008) 1.04
IκB kinase phosphorylation of SNAP-23 controls platelet secretion. Blood (2013) 1.03
Cutting edge: Evidence for a dynamically driven T cell signaling mechanism. J Immunol (2012) 1.03
Mast cell degranulation requires N-ethylmaleimide-sensitive factor-mediated SNARE disassembly. J Immunol (2003) 1.02
Active-site alkylation destabilizes human O6-alkylguanine DNA alkyltransferase. Protein Sci (2004) 1.01
Distinct roles for Rap1b protein in platelet secretion and integrin αIIbβ3 outside-in signaling. J Biol Chem (2011) 1.01
Application of a Saccharomyces cerevisiae model to study requirements for trafficking of Yersinia pestis YopM in eucaryotic cells. Infect Immun (2003) 1.00
A role for Sec1/Munc18 proteins in platelet exocytosis. Biochem J (2003) 1.00
Dissecting the N-ethylmaleimide-sensitive factor: required elements of the N and D1 domains. J Biol Chem (2009) 1.00
Characterization of the pearl oyster (Pinctada martensii) mantle transcriptome unravels biomineralization genes. Mar Biotechnol (NY) (2012) 0.99
Mutation of active site residues of insulin-degrading enzyme alters allosteric interactions. J Biol Chem (2005) 0.97
Primary platelet signaling cascades and integrin-mediated signaling control ADP-ribosylation factor (Arf) 6-GTP levels during platelet activation and aggregation. J Biol Chem (2008) 0.97
Structure and nucleosome interaction of the yeast NuA4 and Piccolo-NuA4 histone acetyltransferase complexes. Nat Struct Mol Biol (2011) 0.97
The impact of Yangtze River discharge, ocean currents and historical events on the biogeographic pattern of Cellana toreuma along the China coast. PLoS One (2012) 0.95
Asymmetric accumulation of hippocampal 7S SNARE complexes occurs regardless of kindling paradigm. Epilepsy Res (2006) 0.95
Borrelia burgdorferi EbfC defines a newly-identified, widespread family of bacterial DNA-binding proteins. Nucleic Acids Res (2009) 0.95
Characterization of nuclear ferritin and mechanism of translocation. Biochem J (2005) 0.95
BpaB, a novel protein encoded by the Lyme disease spirochete's cp32 prophages, binds to erp Operator 2 DNA. Nucleic Acids Res (2010) 0.92
Highly efficient electroosmotic flow through functionalized carbon nanotube membranes. Nanoscale (2011) 0.91
Cell-wall remodeling by the zinc-protease AmpDh3 from Pseudomonas aeruginosa. J Am Chem Soc (2013) 0.91
Uncoupling the ATPase activity of the N-ethylmaleimide sensitive factor (NSF) from 20S complex disassembly. Biochemistry (2002) 0.90
Effects of zinc occupancy on human O6-alkylguanine-DNA alkyltransferase. Biochemistry (2003) 0.90
Interactions of human O(6)-alkylguanine-DNA alkyltransferase (AGT) with short double-stranded DNAs. Biochemistry (2008) 0.90
Platelet secretion and hemostasis require syntaxin-binding protein STXBP5. J Clin Invest (2014) 0.89
Nucleotide-dependent conformational changes in the N-Ethylmaleimide Sensitive Factor (NSF) and their potential role in SNARE complex disassembly. J Struct Biol (2012) 0.89
Interactions and reactions of ferritin with DNA. J Biol Chem (2004) 0.89
6-Carboxyfluorescein and structurally similar molecules inhibit DNA binding and repair by O⁶-alkylguanine DNA alkyltransferase. DNA Repair (Amst) (2011) 0.89
Pro-IL-16 regulation in activated murine CD4+ lymphocytes. J Immunol (2005) 0.88
Levetiracetam prevents kindling-induced asymmetric accumulation of hippocampal 7S SNARE complexes. Epilepsia (2008) 0.87
Topologies of complexes containing O6-alkylguanine-DNA alkyltransferase and DNA. J Mol Biol (2009) 0.86
Trimeric transmembrane domain interactions in paramyxovirus fusion proteins: roles in protein folding, stability, and function. J Biol Chem (2013) 0.85
The Src family kinases and protein kinase C synergize to mediate Gq-dependent platelet activation. J Biol Chem (2012) 0.85
Cooperative cluster formation, DNA bending and base-flipping by O6-alkylguanine-DNA alkyltransferase. Nucleic Acids Res (2012) 0.85
Residues in the hendra virus fusion protein transmembrane domain are critical for endocytic recycling. J Virol (2012) 0.84
Beyond anchoring: the expanding role of the hendra virus fusion protein transmembrane domain in protein folding, stability, and function. J Virol (2012) 0.83
Mutations that probe the cooperative assembly of O⁶-alkylguanine-DNA alkyltransferase complexes. Biochemistry (2011) 0.82
DNA-binding by Haemophilus influenzae and Escherichia coli YbaB, members of a widely-distributed bacterial protein family. BMC Microbiol (2009) 0.82
Plasma membrane Ca(2+)-ATPase (PMCA) translocates to filopodia during platelet activation. Thromb Haemost (2004) 0.82
Studies of secretion using permeabilized platelets. Methods Mol Biol (2004) 0.82
Protein expression in platelets from six species that differ in their open canalicular system. Platelets (2010) 0.81
Proteolytic fragments of insulysin (IDE) retain substrate binding but lose allosteric regulation. Biochemistry (2006) 0.81
Analysis of transcription factor interactions at sedimentation equilibrium. Methods Enzymol (2003) 0.81
Kindling-induced asymmetric accumulation of hippocampal 7S SNARE complexes correlates with enhanced glutamate release. Epilepsia (2011) 0.81
Heterologous stable expression of terpenoid biosynthetic genes using the moss Physcomitrella patens. Methods Mol Biol (2014) 0.81
Cation binding linked to a sequence-specific CAP-DNA interaction. Biophys Chem (2006) 0.80
Lesion-specific DNA-binding and repair activities of human O⁶-alkylguanine DNA alkyltransferase. Nucleic Acids Res (2012) 0.79
Platelets from Munc18c heterozygous mice exhibit normal stimulus-induced release. Thromb Haemost (2004) 0.79
Cysteine 904 is required for maximal insulin degrading enzyme activity and polyanion activation. PLoS One (2012) 0.79
Self-association of the amino-terminal domain of the yeast TATA-binding protein. J Biol Chem (2003) 0.79
Use of DNA length variation to detect periodicities in positively cooperative, nonspecific binding. Methods Enzymol (2009) 0.78
Platelet activation and its patient-specific consequences. Thromb Res (2007) 0.78
Murine platelets are not regulated by O-linked beta-N-acetylglucosamine. Arch Biochem Biophys (2008) 0.78
Histidine-tag-directed chromophores for tracer analyses in the analytical ultracentrifuge. Methods (2010) 0.77
Crystallization of hepatocyte nuclear factor 4 alpha (HNF4 alpha) in complex with the HNF1 alpha promoter element. Acta Crystallogr Sect F Struct Biol Cryst Commun (2008) 0.77
Ionic rectification by electrostatically actuated tethers on single walled carbon nanotube membranes. Chem Commun (Camb) (2012) 0.77
The Escherichia coli cyclic AMP receptor protein forms a 2:2 complex with RNA polymerase holoenzyme, in vitro. J Biol Chem (2002) 0.77
Demonstration of differential quantitative requirements for NSF among multiple vesicle fusion pathways of GLUT4 using a dominant-negative ATPase-deficient NSF. Biochem Biophys Res Commun (2005) 0.77
Role of electrostatic interactions in binding of thrombin to the fibrinogen γ' chain. Biochemistry (2012) 0.76
Thiosulfinates modulate platelet activation by reaction with surface free sulfhydryls and internal thiol-containing proteins. Platelets (2007) 0.76
Circulating MicroRNA-21 Is Involved in Lymph Node Metastasis in Cervical Cancer by Targeting RASA1. Int J Gynecol Cancer (2016) 0.76
Screening for renal cell carcinoma using ultrasonography: a feasibility study. BJU Int (2003) 0.75
The ins and outs of endocytic trafficking in platelet functions. Curr Opin Hematol (2017) 0.75
Isolation and characterization of microsatellite loci in Babylonia areolata and cross-species amplification in Babylonia formosae habei. Mol Ecol Resour (2009) 0.75
[Comparative study on the value of digital radiography and spiral CT scan in the evaluation of adenoidal hypertrophy]. Zhongguo Dang Dai Er Ke Za Zhi (2009) 0.75
[STUDY ON WOUND HEALING AFTER Sommerlad TECHNIQUE REPAIR OF ISOLATED CLEFT PALATE]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2014) 0.75