Published in PLoS One on October 18, 2010
Next-generation transcriptome assembly. Nat Rev Genet (2011) 5.89
A cloud-compatible bioinformatics pipeline for ultrarapid pathogen identification from next-generation sequencing of clinical samples. Genome Res (2014) 3.43
The perils of pathogen discovery: origin of a novel parvovirus-like hybrid genome traced to nucleic acid extraction spin columns. J Virol (2013) 3.37
Actionable diagnosis of neuroleptospirosis by next-generation sequencing. N Engl J Med (2014) 2.71
A novel rhabdovirus associated with acute hemorrhagic fever in central Africa. PLoS Pathog (2012) 2.63
Application of next-generation sequencing technologies in virology. J Gen Virol (2012) 1.98
Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis. Genome Med (2015) 1.69
Applications of next-generation sequencing technologies to diagnostic virology. Int J Mol Sci (2011) 1.64
Cross-species transmission of a novel adenovirus associated with a fulminant pneumonia outbreak in a new world monkey colony. PLoS Pathog (2011) 1.62
Discovery of a novel polyomavirus in acute diarrheal samples from children. PLoS One (2012) 1.48
Unbiased parallel detection of viral pathogens in clinical samples by use of a metagenomic approach. J Clin Microbiol (2011) 1.41
Retracted Metagenomic Human Repiratory Air in a Hospital Environment. PLoS One (2015) 1.39
In-depth investigation of archival and prospectively collected samples reveals no evidence for XMRV infection in prostate cancer. PLoS One (2012) 1.33
The genome sequence of Lone Star virus, a highly divergent bunyavirus found in the Amblyomma americanum tick. PLoS One (2013) 1.30
Comparing viral metagenomics methods using a highly multiplexed human viral pathogens reagent. J Virol Methods (2014) 1.20
Genome sequencing in clinical microbiology. Nat Biotechnol (2012) 1.20
2009 pandemic H1N1 influenza virus elicits similar clinical course but differential host transcriptional response in mouse, macaque, and swine infection models. BMC Genomics (2012) 1.13
High-throughput RNA sequencing of a formalin-fixed, paraffin-embedded autopsy lung tissue sample from the 1918 influenza pandemic. J Pathol (2013) 1.09
Metagenomics for pathogen detection in public health. Genome Med (2013) 1.03
Evolution of mosquito-based arbovirus surveillance systems in Australia. J Biomed Biotechnol (2012) 1.02
Animal virus discovery: improving animal health, understanding zoonoses, and opportunities for vaccine development. Curr Opin Virol (2012) 1.01
Diagnostic metagenomics: potential applications to bacterial, viral and parasitic infections. Parasitology (2014) 0.99
Rapid metagenomic next-generation sequencing during an investigation of hospital-acquired human parainfluenza virus 3 infections. J Clin Microbiol (2016) 0.98
Clinical, genomic, and metagenomic characterization of oral tongue squamous cell carcinoma in patients who do not smoke. Head Neck (2014) 0.98
A novel adenovirus species associated with an acute respiratory outbreak in a baboon colony and evidence of coincident human infection. MBio (2013) 0.98
Identification of a novel bat papillomavirus by metagenomics. PLoS One (2012) 0.97
Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014. Emerg Infect Dis (2015) 0.95
The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing. PLoS One (2013) 0.94
The role of metagenomics in understanding the human microbiome in health and disease. Virulence (2014) 0.93
Characterization of the upper respiratory tract microbiomes of patients with pandemic H1N1 influenza. PLoS One (2013) 0.91
Molecular epidemiology of influenza A/H3N2 viruses circulating in Uganda. PLoS One (2011) 0.90
From orphan virus to pathogen: the path to the clinical lab. Curr Opin Virol (2011) 0.90
Clinical PathoScope: rapid alignment and filtration for accurate pathogen identification in clinical samples using unassembled sequencing data. BMC Bioinformatics (2014) 0.90
Quantitative characterization of defective virus emergence by deep sequencing. J Virol (2013) 0.89
Evaluation of Unbiased Next-Generation Sequencing of RNA (RNA-seq) as a Diagnostic Method in Influenza Virus-Positive Respiratory Samples. J Clin Microbiol (2015) 0.89
Genetic diversity among pandemic 2009 influenza viruses isolated from a transmission chain. Virol J (2013) 0.87
A Pan-HIV Strategy for Complete Genome Sequencing. J Clin Microbiol (2015) 0.87
Idiopathic pneumonia syndrome after hematopoietic cell transplantation: evidence of occult infectious etiologies. Blood (2015) 0.86
Recent advances in diagnosis, prevention, and treatment of human respiratory syncytial virus. Adv Virol (2013) 0.84
Molecular evolutionary analysis of pH1N1 2009 influenza virus in Reunion Island, South West Indian Ocean region: a cohort study. PLoS One (2012) 0.84
Current Approaches for Diagnosis of Influenza Virus Infections in Humans. Viruses (2016) 0.84
Utility of Metagenomic Next-Generation Sequencing for Characterization of HIV and Human Pegivirus Diversity. PLoS One (2015) 0.81
The administration of intranasal live attenuated influenza vaccine induces changes in the nasal microbiota and nasal epithelium gene expression profiles. Microbiome (2015) 0.80
The human microbiota: a new direction in the investigation of thoracic diseases. J Thorac Dis (2013) 0.80
Evaluation of the broad-range PCR-electrospray ionization mass spectrometry (PCR/ESI-MS) system and virus microarrays for virus detection. Viruses (2014) 0.80
Using high-throughput sequencing to leverage surveillance of genetic diversity and oseltamivir resistance: a pilot study during the 2009 influenza A(H1N1) pandemic. PLoS One (2013) 0.79
Next-generation sequencing diagnostics of bacteremia in septic patients. Genome Med (2016) 0.78
An easy operating pathogen microarray (EOPM) platform for rapid screening of vertebrate pathogens. BMC Infect Dis (2013) 0.77
A new look at an old virus: patterns of mutation accumulation in the human H1N1 influenza virus since 1918. Theor Biol Med Model (2012) 0.77
Metagenomics: A New Way to Illustrate the Crosstalk between Infectious Diseases and Host Microbiome. Int J Mol Sci (2015) 0.76
No viral association found in a set of differentiated vulvar intraepithelial neoplasia cases by human papillomavirus and pan-viral microarray testing. PLoS One (2015) 0.75
Genetic and Epidemiologic Trends of Norovirus Outbreaks in the United States from 2013 to 2016 Demonstrated Emergence of Novel GII.4 Recombinant Viruses. J Clin Microbiol (2017) 0.75
A comparative study of human TLR 7/8 stimulatory trimer compositions in influenza A viral genomes. PLoS One (2012) 0.75
DNA Microarray Platform for Detection and Surveillance of Viruses Transmitted by Small Mammals and Arthropods. PLoS Negl Trop Dis (2016) 0.75
Copy number heterogeneity of JC virus standards. J Clin Microbiol (2016) 0.75
A Multicenter Study To Evaluate the Performance of High-Throughput Sequencing for Virus Detection. mSphere (2017) 0.75
SSAHA: a fast search method for large DNA databases. Genome Res (2001) 48.64
Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med (2009) 42.75
Real-time DNA sequencing from single polymerase molecules. Science (2008) 29.53
Substantial biases in ultra-short read data sets from high-throughput DNA sequencing. Nucleic Acids Res (2008) 26.36
Pandemic potential of a strain of influenza A (H1N1): early findings. Science (2009) 26.24
The NIH Human Microbiome Project. Genome Res (2009) 24.61
Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science (2003) 18.34
Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic. Nature (2009) 17.96
Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science (2008) 16.60
Antiviral actions of interferons. Clin Microbiol Rev (2001) 13.06
Retracted Detection of an infectious retrovirus, XMRV, in blood cells of patients with chronic fatigue syndrome. Science (2009) 10.76
Retracted Identification of a novel Gammaretrovirus in prostate tumors of patients homozygous for R462Q RNASEL variant. PLoS Pathog (2006) 10.08
Multiple sequence alignment using ClustalW and ClustalX. Curr Protoc Bioinformatics (2002) 9.86
Triple-reassortant swine influenza A (H1) in humans in the United States, 2005-2009. N Engl J Med (2009) 9.06
A new arenavirus in a cluster of fatal transplant-associated diseases. N Engl J Med (2008) 7.73
Microarray-based detection and genotyping of viral pathogens. Proc Natl Acad Sci U S A (2002) 7.27
Rapid-test sensitivity for novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med (2009) 5.98
Viral discovery and sequence recovery using DNA microarrays. PLoS Biol (2003) 5.95
Severe 2009 H1N1 influenza in pregnant and postpartum women in California. N Engl J Med (2009) 5.35
RNA viral community in human feces: prevalence of plant pathogenic viruses. PLoS Biol (2006) 4.93
Genetic detection and characterization of Lujo virus, a new hemorrhagic fever-associated arenavirus from southern Africa. PLoS Pathog (2009) 4.88
Pan-viral screening of respiratory tract infections in adults with and without asthma reveals unexpected human coronavirus and human rhinovirus diversity. J Infect Dis (2007) 4.16
Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis. J Virol (2009) 4.14
Panmicrobial oligonucleotide array for diagnosis of infectious diseases. Emerg Infect Dis (2007) 3.51
Metagenomic analysis of respiratory tract DNA viral communities in cystic fibrosis and non-cystic fibrosis individuals. PLoS One (2009) 3.41
Kleine-Levin syndrome: a systematic study of 108 patients. Ann Neurol (2008) 3.03
Bat guano virome: predominance of dietary viruses from insects and plants plus novel mammalian viruses. J Virol (2010) 3.03
Identification of a novel astrovirus (astrovirus VA1) associated with an outbreak of acute gastroenteritis. J Virol (2009) 2.96
Identification of cardioviruses related to Theiler's murine encephalomyelitis virus in human infections. Proc Natl Acad Sci U S A (2008) 2.84
PANTHER pathway: an ontology-based pathway database coupled with data analysis tools. Methods Mol Biol (2009) 2.76
Multiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces. J Virol (2009) 2.70
Recovery of divergent avian bornaviruses from cases of proventricular dilatation disease: identification of a candidate etiologic agent. Virol J (2008) 2.69
Direct metagenomic detection of viral pathogens in nasal and fecal specimens using an unbiased high-throughput sequencing approach. PLoS One (2009) 2.67
Streptococcus pneumoniae coinfection is correlated with the severity of H1N1 pandemic influenza. PLoS One (2009) 2.65
Development and quantitative analyses of a universal rRNA-subtraction protocol for microbial metatranscriptomics. ISME J (2010) 2.54
The complete genome of klassevirus - a novel picornavirus in pediatric stool. Virol J (2009) 2.46
Broad-spectrum respiratory tract pathogen identification using resequencing DNA microarrays. Genome Res (2006) 2.11
Detection of molecular markers of drug resistance in 2009 pandemic influenza A (H1N1) viruses by pyrosequencing. Antimicrob Agents Chemother (2009) 2.09
E-Predict: a computational strategy for species identification based on observed DNA microarray hybridization patterns. Genome Biol (2005) 1.93
Microarray detection of human parainfluenzavirus 4 infection associated with respiratory failure in an immunocompetent adult. Clin Infect Dis (2006) 1.84
Inefficient control of host gene expression by the 2009 pandemic H1N1 influenza A virus NS1 protein. J Virol (2010) 1.81
Experimental evaluation of the FluChip diagnostic microarray for influenza virus surveillance. J Clin Microbiol (2006) 1.76
Influenza vaccine--outmaneuvering antigenic shift and drift. N Engl J Med (2004) 1.72
Metagenomics for the discovery of novel human viruses. Future Microbiol (2010) 1.65
Characterization of quasispecies of pandemic 2009 influenza A virus (A/H1N1/2009) by de novo sequencing using a next-generation DNA sequencer. PLoS One (2010) 1.56
Identifying influenza viruses with resequencing microarrays. Emerg Infect Dis (2006) 1.45
Xenotropic murine leukemia virus-related gammaretrovirus in respiratory tract. Emerg Infect Dis (2010) 1.40
The long march: a sample preparation technique that enhances contig length and coverage by high-throughput short-read sequencing. PLoS One (2008) 1.31
Utility of DNA microarrays for detection of viruses in acute respiratory tract infections in children. J Pediatr (2008) 1.26
Robust sequence selection method used to develop the FluChip diagnostic microarray for influenza virus. J Clin Microbiol (2006) 1.25
Detection in 2009 of the swine origin influenza A (H1N1) virus by a subtyping microarray. J Clin Microbiol (2009) 1.00
The next generation becomes the now generation. PLoS Genet (2010) 0.96
Large-scale evolutionary surveillance of the 2009 H1N1 influenza A virus using resequencing arrays. Nucleic Acids Res (2010) 0.85
Achalasia and viral infection: new insights from veterinary medicine. Sci Transl Med (2010) 0.81
Empirical analysis of transcriptional activity in the Arabidopsis genome. Science (2003) 11.62
Rapid pneumococcal evolution in response to clinical interventions. Science (2011) 9.09
Clinical features and short-term outcomes of 144 patients with SARS in the greater Toronto area. JAMA (2003) 8.76
Conserved patterns of protein interaction in multiple species. Proc Natl Acad Sci U S A (2005) 7.48
The immediate psychological and occupational impact of the 2003 SARS outbreak in a teaching hospital. CMAJ (2003) 7.46
Whole-genome sequencing and social-network analysis of a tuberculosis outbreak. N Engl J Med (2011) 7.35
Expanding access to testicular tissue cryopreservation: an analysis by analogy. Am J Bioeth (2013) 7.01
Conserved pathways within bacteria and yeast as revealed by global protein network alignment. Proc Natl Acad Sci U S A (2003) 4.68
Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19. Plant Cell (2005) 4.41
Before-after study of a standardized hospital order set for the management of septic shock. Crit Care Med (2006) 4.10
Expansion of a unique CD57⁺NKG2Chi natural killer cell subset during acute human cytomegalovirus infection. Proc Natl Acad Sci U S A (2011) 3.53
The perils of pathogen discovery: origin of a novel parvovirus-like hybrid genome traced to nucleic acid extraction spin columns. J Virol (2013) 3.37
Podoplanin: a novel marker for oral cancer risk in patients with oral premalignancy. J Clin Oncol (2008) 3.16
Negative mucosal synergy between Herpes simplex type 2 and HIV in the female genital tract. AIDS (2007) 3.14
West Nile virus infection in 2002: morbidity and mortality among patients admitted to hospital in southcentral Ontario. CMAJ (2003) 3.03
Ceftiofur resistance in Salmonella enterica serovar Heidelberg from chicken meat and humans, Canada. Emerg Infect Dis (2010) 3.01
Actionable diagnosis of neuroleptospirosis by next-generation sequencing. N Engl J Med (2014) 2.71
Recovery of divergent avian bornaviruses from cases of proventricular dilatation disease: identification of a candidate etiologic agent. Virol J (2008) 2.69
A novel rhabdovirus associated with acute hemorrhagic fever in central Africa. PLoS Pathog (2012) 2.63
Effectiveness of AS03 adjuvanted pandemic H1N1 vaccine: case-control evaluation based on sentinel surveillance system in Canada, autumn 2009. BMJ (2011) 2.50
Health care-associated pneumonia and community-acquired pneumonia: a single-center experience. Antimicrob Agents Chemother (2007) 2.49
The complete genome of klassevirus - a novel picornavirus in pediatric stool. Virol J (2009) 2.46
A potent dauer pheromone component in Caenorhabditis elegans that acts synergistically with other components. Proc Natl Acad Sci U S A (2008) 2.35
Functional genomic analysis of the AUXIN/INDOLE-3-ACETIC ACID gene family members in Arabidopsis thaliana. Plant Cell (2005) 2.27
Science communication. Interactions with the mass media. Science (2008) 2.20
Assessment of the ability of a fourth-generation immunoassay for human immunodeficiency virus (HIV) antibody and p24 antigen to detect both acute and recent HIV infections in a high-risk setting. J Clin Microbiol (2009) 2.18
Unresectable solitary hepatocellular carcinoma not amenable to radiofrequency ablation: multicenter radiology-pathology correlation and survival of radiation segmentectomy. Hepatology (2014) 2.14
Chloroquine-resistant Plasmodium vivax malaria in Peru. Am J Trop Med Hyg (2003) 2.12
Treatment-related risk factors for hospital mortality in Candida bloodstream infections. Crit Care Med (2008) 2.06
Detection of airborne severe acute respiratory syndrome (SARS) coronavirus and environmental contamination in SARS outbreak units. J Infect Dis (2005) 2.03
Performance of the Celera Diagnostics ViroSeq HIV-1 Genotyping System for sequence-based analysis of diverse human immunodeficiency virus type 1 strains. J Clin Microbiol (2004) 2.01
TIPS for refractory ascites: a 6-year single-center experience with expanded polytetrafluoroethylene-covered stent-grafts. AJR Am J Roentgenol (2015) 1.99
E-Predict: a computational strategy for species identification based on observed DNA microarray hybridization patterns. Genome Biol (2005) 1.93
Evaluation of the RealArt Malaria LC real-time PCR assay for malaria diagnosis. J Clin Microbiol (2004) 1.91
No evidence of murine-like gammaretroviruses in CFS patients previously identified as XMRV-infected. Science (2011) 1.90
Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR. Nucleic Acids Res (2007) 1.82
Evaluation of the sensitivity and specificity of six HIV combined p24 antigen and antibody assays. J Virol Methods (2004) 1.82
Use of a multifaceted approach to analyze HIV incidence in a cohort study of women in the United States: HIV Prevention Trials Network 064 Study. J Infect Dis (2012) 1.78
Transmission of severe acute respiratory syndrome during intubation and mechanical ventilation. Am J Respir Crit Care Med (2004) 1.78
The laboratory impact of changing syphilis screening from the rapid-plasma reagin to a treponemal enzyme immunoassay: a case-study from the Greater Toronto Area. Sex Transm Dis (2011) 1.77
Success of self-administered home fecal transplantation for chronic Clostridium difficile infection. Clin Gastroenterol Hepatol (2010) 1.77
The Plasmodium protein network diverges from those of other eukaryotes. Nature (2005) 1.76
Impact of human immunodeficiency virus type 1 (HIV-1) genetic diversity on performance of four commercial viral load assays: LCx HIV RNA Quantitative, AMPLICOR HIV-1 MONITOR v1.5, VERSANT HIV-1 RNA 3.0, and NucliSens HIV-1 QT. J Clin Microbiol (2005) 1.75
Sex determining region Y-Box 2 (SOX2) is a potential cell-lineage gene highly expressed in the pathogenesis of squamous cell carcinomas of the lung. PLoS One (2010) 1.69
Cross-species transmission of a novel adenovirus associated with a fulminant pneumonia outbreak in a new world monkey colony. PLoS Pathog (2011) 1.62
Multistep entry of rotavirus into cells: a Versaillesque dance. Trends Microbiol (2004) 1.62
The impact of infection on population health: results of the Ontario burden of infectious diseases study. PLoS One (2012) 1.58
Disproportionately high semen shedding of HIV is associated with compartmentalized cytomegalovirus reactivation. J Infect Dis (2005) 1.58
Novel susceptibility loci for second primary tumors/recurrence in head and neck cancer patients: large-scale evaluation of genetic variants. Cancer Prev Res (Phila) (2009) 1.57
Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies. Retrovirology (2010) 1.57
Absence of the CAAX endoprotease Rce1: effects on cell growth and transformation. Mol Cell Biol (2002) 1.56
Disruption of the Salmonella-containing vacuole leads to increased replication of Salmonella enterica serovar typhimurium in the cytosol of epithelial cells. Infect Immun (2002) 1.56
Failure to confirm XMRV/MLVs in the blood of patients with chronic fatigue syndrome: a multi-laboratory study. Science (2011) 1.56
Silencing the morphogenesis of rotavirus. J Virol (2005) 1.55
The incidence and effect of bacteriospermia and elevated seminal leukocytes on semen parameters. Fertil Steril (2012) 1.55
Prevalence and predictors of lipid and glucose monitoring in commercially insured patients treated with second-generation antipsychotic agents. Am J Psychiatry (2009) 1.55
The moderating impact of ethnicity on metabolic outcomes during treatment with olanzapine and aripiprazole in patients with schizophrenia. J Clin Psychiatry (2008) 1.54
Global assessment of genetic variation influencing response to retinoid chemoprevention in head and neck cancer patients. Cancer Prev Res (Phila) (2011) 1.53
Rotavirus vaccine: early introduction in Latin America-risks and benefits. Arch Med Res (2006) 1.53
Interpretation of diagnostic laboratory tests for severe acute respiratory syndrome: the Toronto experience. CMAJ (2004) 1.50
Rotavirus infection induces the phosphorylation of eIF2alpha but prevents the formation of stress granules. J Virol (2007) 1.49
A combinatorial interplay among the 1-aminocyclopropane-1-carboxylate isoforms regulates ethylene biosynthesis in Arabidopsis thaliana. Genetics (2009) 1.49
Discovery of a novel polyomavirus in acute diarrheal samples from children. PLoS One (2012) 1.48
Validation of the risk index category as a predictor of surgical site infection in elective colorectal surgery. Dis Colon Rectum (2010) 1.47
Application of site and haplotype-frequency based approaches for detecting selection signatures in cattle. BMC Genomics (2011) 1.47
A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A-H, group O and group N samples. J Virol Methods (2007) 1.47
Characterization of rotavirus cell entry. J Virol (2004) 1.46
Detection of individuals with acute HIV-1 infection using the ARCHITECT HIV Ag/Ab Combo assay. J Acquir Immune Defic Syndr (2009) 1.46
The prevalence of diverse HIV-1 strains was stable in Cameroonian blood donors from 1996 to 2004. J Acquir Immune Defic Syndr (2008) 1.46
Rubella outbreak in the Netherlands, 2004-2005: high burden of congenital infection and spread to Canada. Pediatr Infect Dis J (2009) 1.45
Seasonal influenza vaccine and increased risk of pandemic A/H1N1‐related illness: first detection of the association in British Columbia, Canada. Clin Infect Dis (2010) 1.43
Estimation of HIV incidence in a large, community-based, randomized clinical trial: NIMH project accept (HIV Prevention Trials Network 043). PLoS One (2013) 1.40
The value of the dedicated orthopaedic trauma operating room. J Trauma (2006) 1.40
Performance of the xTAG® gastrointestinal pathogen panel, a multiplex molecular assay for simultaneous detection of bacterial, viral, and parasitic causes of infectious gastroenteritis. J Microbiol Biotechnol (2013) 1.39
Creating a medical futility policy. Physicians at a Honolulu hospital describe the experience. Health Prog (2003) 1.38
Heat shock cognate protein 70 is involved in rotavirus cell entry. J Virol (2002) 1.37
The characteristics of NDM-producing Klebsiella pneumoniae from Canada. Diagn Microbiol Infect Dis (2011) 1.37
Rotavirus gene silencing by small interfering RNAs. EMBO Rep (2002) 1.36
Toward an understanding of changes in diversity associated with fecal microbiome transplantation based on 16S rRNA gene deep sequencing. MBio (2012) 1.36
High-level beta-globin expression and preferred intragenic integration after lentiviral transduction of human cord blood stem cells. J Clin Invest (2004) 1.35
A stability limit for the atmospheres of giant extrasolar planets. Nature (2007) 1.35
In-depth investigation of archival and prospectively collected samples reveals no evidence for XMRV infection in prostate cancer. PLoS One (2012) 1.33
Performance of the automated Abbott RealTime HIV-1 assay on a genetically diverse panel of specimens from Brazil. J Virol Methods (2006) 1.33
Self-collected mid-turbinate swabs for the detection of respiratory viruses in adults with acute respiratory illnesses. PLoS One (2011) 1.33