Published in J Exp Med on March 01, 1990
The 3D structure of the immunoglobulin heavy-chain locus: implications for long-range genomic interactions. Cell (2008) 3.92
Pax5 induces V-to-DJ rearrangements and locus contraction of the immunoglobulin heavy-chain gene. Genes Dev (2004) 3.66
Most peripheral B cells in mice are ligand selected. J Exp Med (1991) 3.50
Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences. J Exp Med (1990) 2.83
CTCF-binding elements mediate control of V(D)J recombination. Nature (2011) 2.62
Balancing immunity and tolerance: deleting and tuning lymphocyte repertoires. Proc Natl Acad Sci U S A (1996) 2.34
Transitional B cells are the target of negative selection in the B cell compartment. J Exp Med (1995) 2.26
Stepwise activation of the immunoglobulin mu heavy chain gene locus. EMBO J (2001) 2.14
Cis-regulatory elements and epigenetic changes control genomic rearrangements of the IgH locus. Adv Immunol (2008) 1.70
Determinism and stochasticity during maturation of the zebrafish antibody repertoire. Proc Natl Acad Sci U S A (2011) 1.68
Clonal analysis of a human antibody response. II. Sequences of the VH genes of human IgM, IgG, and IgA to rabies virus reveal preferential utilization of VHIII segments and somatic hypermutation. J Immunol (1993) 1.65
Chromatin architecture and the generation of antigen receptor diversity. Cell (2009) 1.59
Changes in histone acetylation are associated with differences in accessibility of V(H) gene segments to V-DJ recombination during B-cell ontogeny and development. Mol Cell Biol (2003) 1.50
Syk tyrosine kinase is required for the positive selection of immature B cells into the recirculating B cell pool. J Exp Med (1997) 1.46
Immunoglobulin heavy chain gene expression in peripheral blood B lymphocytes. J Clin Invest (1992) 1.46
A B-cell receptor-specific selection step governs immature to mature B cell differentiation. Proc Natl Acad Sci U S A (2000) 1.42
Ordered rearrangement of variable region genes of the T cell receptor gamma locus correlates with transcription of the unrearranged genes. J Exp Med (1993) 1.36
Molecular and idiotypic analysis of antibodies to Cryptococcus neoformans glucuronoxylomannan. Infect Immun (1994) 1.34
B-1 B lymphocytes require Blimp-1 for immunoglobulin secretion. J Exp Med (2006) 1.21
A novel mechanism for B cell repertoire maturation based on response by B cell precursors to pre-B receptor assembly. J Exp Med (1998) 1.16
V-region directed selection in differentiating B lymphocytes. EMBO J (1993) 1.07
CTCF-binding elements 1 and 2 in the Igh intergenic control region cooperatively regulate V(D)J recombination. Proc Natl Acad Sci U S A (2015) 1.05
Sequence of the spacer in the recombination signal sequence affects V(D)J rearrangement frequency and correlates with nonrandom Vkappa usage in vivo. J Exp Med (1998) 1.04
The T cell receptor repertoire influences V beta element usage in response to myoglobin. J Exp Med (1991) 1.04
Regulation of the antibody repertoire through control of HCDR3 diversity. Vaccine (1998) 1.02
Allelic exclusion of the immunoglobulin heavy chain locus is independent of its nuclear localization in mature B cells. Nucleic Acids Res (2013) 0.98
Deep sequencing of the murine IgH repertoire reveals complex regulation of nonrandom V gene rearrangement frequencies. J Immunol (2013) 0.98
Normal serum immunoglobulins participate in the selection of peripheral B-cell repertoires. Proc Natl Acad Sci U S A (1991) 0.98
Organization, complexity and allelic diversity of the porcine (Sus scrofa domestica) immunoglobulin lambda locus. Immunogenetics (2011) 0.97
Organization of lamprey variable lymphocyte receptor C locus and repertoire development. Proc Natl Acad Sci U S A (2013) 0.96
The transition of pre-BI to pre-BII cells is dependent on the VH structure of the mu/surrogate L chain receptor. EMBO J (1996) 0.96
Clinical implications of the specialised B cell response to polysaccharide encapsulated pathogens. Postgrad Med J (2001) 0.95
The peritoneal cavity B-2 antibody repertoire appears to reflect many of the same selective pressures that shape the B-1a and B-1b repertoires. J Immunol (2010) 0.90
Characteristics of Borrelia hermsii infection in human hematopoietic stem cell-engrafted mice mirror those of human relapsing fever. Proc Natl Acad Sci U S A (2011) 0.90
Selection of antigen-specific, idiotype-positive B cells in transgenic mice expressing a rearranged M167-mu heavy chain gene. J Exp Med (1991) 0.90
Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires. Elife (2015) 0.89
The genomic arrangement of T cell receptor variable genes is a determinant of the developmental rearrangement pattern. Proc Natl Acad Sci U S A (2003) 0.88
Differential expression of VH gene families in peripheral B cell repertoires of newborn or adult immunoglobulin H chain congenic mice. J Exp Med (1992) 0.86
Susceptibility to multiple sclerosis is associated with the proximal immunoglobulin heavy chain variable region. J Clin Invest (1991) 0.86
Molecular characterization of VDJ transcripts from a newborn piglet. Immunology (1996) 0.84
Two distinct populations of H chain-edited B cells show differential surrogate L chain dependence. J Immunol (2009) 0.82
Immunoglobulin heavy chain variable region gene utilization by B cell hybridomas derived from rheumatoid synovial tissue. Clin Exp Immunol (1992) 0.81
Highly sensitive and unbiased approach for elucidating antibody repertoires. Proc Natl Acad Sci U S A (2016) 0.80
The genomic organization of immunoglobulin VH genes in Xenopus laevis shows evidence for interspersion of families. Nucleic Acids Res (1991) 0.80
CNOT3 contributes to early B cell development by controlling Igh rearrangement and p53 mRNA stability. J Exp Med (2015) 0.80
Molecular characterization of J558 genes encoding tight-skin mouse autoantibodies: identical heavy-chain variable genes code for antibodies with different specificities. Proc Natl Acad Sci U S A (1994) 0.75
The second century of the antibody. Molecular perspectives in regulation, pathophysiology, and therapeutic applications. West J Med (1992) 0.75
Somatic generation of antibody diversity. Nature (1983) 28.06
A severe combined immunodeficiency mutation in the mouse. Nature (1983) 13.99
Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways. Cell (1980) 9.86
Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain. Cell (1980) 6.32
Developmentally controlled and tissue-specific expression of unrearranged VH gene segments. Cell (1985) 6.21
Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell lines. Nature (1984) 5.75
The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice. J Exp Med (1983) 5.74
Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3' ends. Cell (1980) 5.59
The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse. I. One hundred Igh-V genes comprise seven families of homologous genes. Eur J Immunol (1984) 5.33
Rearrangement of antigen receptor genes is defective in mice with severe combined immune deficiency. Cell (1986) 4.68
Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines. Mol Cell Biol (1982) 4.26
Genetics of a new IgVH (T15 idiotype) marker in the mouse regulating natural antibody to phosphorylcholine. J Exp Med (1974) 3.64
Evidence of functional lymphocytes in some (leaky) scid mice. J Exp Med (1988) 3.09
Characterization of productive and sterile transcripts from the immunoglobulin heavy-chain locus: processing of micron and muS mRNA. Mol Cell Biol (1983) 3.00
The scid defect affects the final step of the immunoglobulin VDJ recombinase mechanism. Cell (1988) 2.71
The characterization fo the B-cell repertoire specific for the 2,4-dinitrophenyl and 2,4,6-trinitrophenyl determinants in neonatal BALB/c mice. J Exp Med (1975) 2.32
VH-gene expression in murine lipopolysaccharide blasts distributes over the nine known VH-gene groups and may be random. Eur J Immunol (1985) 2.03
VHDJH formation and DJH replacement during pre-B differentiation: non-random usage of gene segments. EMBO J (1986) 1.99
Population dynamics of bone marrow B lymphocytes. Immunol Rev (1986) 1.94
Transcripts of the immunoglobulin C mu gene vary in structure and splicing during lymphoid development. Proc Natl Acad Sci U S A (1980) 1.89
Developmentally regulated and strain-specific expression of murine VH gene families. J Exp Med (1988) 1.85
VH gene family utilization in colonies derived from B and pre-B cells detected by the RNA colony blot assay. EMBO J (1986) 1.77
Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes. EMBO J (1985) 1.75
Restricted Ig variable region gene expression among Ly-1+ B cell lymphomas. J Immunol (1988) 1.72
One heavy chain variable region gene segment subfamily in the BALB/c mouse contains 500-1000 or more members. Cell (1986) 1.60
During B-cell differentiation enhancer activity and transcription rate of immunoglobulin heavy chain genes are high before mRNA accumulation. Cell (1986) 1.59
The generation of diversity in phosphorylcholine-binding antibodies. Adv Immunol (1984) 1.59
Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice. J Exp Med (1987) 1.49
Adult B-cell repertoire is biased toward two heavy-chain variable-region genes that rearrange frequently in fetal pre-B cells. Proc Natl Acad Sci U S A (1987) 1.46
Synthesis of immunoglobulin mu chain gene products precedes synthesis of light chains during B-lymphocyte development. Proc Natl Acad Sci U S A (1981) 1.44
Organization of genes and spacers within the mouse immunoglobulin VH locus. J Mol Appl Genet (1981) 1.41
Comparison of the fetal and adult functional B cell repertoires by analysis of VH gene family expression. J Exp Med (1988) 1.38
The mechanism of thymus-dependent antibody formation in bone marrow. J Immunol (1981) 1.37
The organization of the mouse Igh-V locus. Dispersion, interspersion, and the evolution of VH gene family clusters. J Exp Med (1988) 1.36
Immune response to levan. III. The capacity to produce anti-inulin antibodies and cross-reactive idiotypes appears late in ontogeny. J Immunol (1979) 1.34
A member of a new VH gene family encodes antibromelinized mouse red blood cell autoantibodies. Eur J Immunol (1988) 1.28
Patterned acquisition of the antibody repertoire: diversity of the hemagglutinin-specific B-cell repertoire in neonatal BALB/c mice. Proc Natl Acad Sci U S A (1979) 1.24
Specificities and V genes encoding monoclonal autoantibodies from viable motheaten mice. J Exp Med (1988) 1.23
Antibody repertoires of normal BALB/c mice: B lymphocyte populations defined by state of activation. Immunol Rev (1986) 1.22
Organization of the murine immunoglobulin VH complex in the inbred strains. EMBO J (1987) 1.22
Murine V kappa gene expression does not follow the VH paradigm. J Exp Med (1989) 1.19
Functional characterization of monoclonal auto-anti-idiotype antibodies isolated from the early B cell repertoire of BALB/c mice. Eur J Immunol (1986) 1.15
Preferential expression of variable region heavy chain gene segments by predominant 2,4-dinitrophenyl-specific BALB/c neonatal antibody clonotypes. Proc Natl Acad Sci U S A (1986) 1.13
Immunological unresponsiveness to native dextran B512 in young animals of dextran high responder strains is due to lack of Ig receptors expression. Evidence for a nonrandom expression of V-genes. J Exp Med (1978) 1.10
Strain-dependent expression of VH gene families. J Immunol (1988) 1.07
A new murine Ig VH gene family. J Immunol (1988) 1.06
Ontogeny of B cell precursors responding to alpha 1- greater than 3 dextran in BALB/c mice. J Immunol (1984) 1.04
Immunoglobulin VH region genes of the mouse are organized in overlapping clusters. Eur J Immunol (1987) 1.04
An immunofluorescence analysis of the ontogeny of myeloid, T, and B lineage cells in mouse hemopoietic tissues. J Immunol (1984) 1.03
Selective use of the VHQ52 family in functional VH to DJH rearrangements in a B precursor cell line. J Exp Med (1987) 1.02
High connectivity, natural antibodies preferentially use 7183 and QUPC 52 VH families. Eur J Immunol (1987) 0.97
VH gene family utilization is regulated by a locus outside of the VH region. J Exp Med (1988) 0.96
Highly preferential VH-VL pairing in normal B cells results in antigen-independent selection of the available repertoire. J Immunol (1987) 0.96
Late clonal selection and expansion of the TEPC-15 germ-line specificity. J Exp Med (1980) 0.92
Ig gene rearrangement and expression in the progeny of B-cell progenitors in the course of clonal expansion in bone marrow cultures. EMBO J (1987) 0.92
The effect of the scid mutation on mechanism and control of immunoglobulin heavy and light chain gene rearrangement. Curr Top Microbiol Immunol (1989) 0.89
Genetic and temporal control of neonatal antibody expression. J Exp Med (1983) 0.89
The ontogeny and organization of the lymphoid system. J Invest Dermatol (1985) 0.86
Isotype, VH genes, and antigen-binding analysis of hybridomas from newborn normal BALB/c mice. J Immunol (1988) 0.84
B cell immune repertoire diversifies in a predictable temporal order in vitro. J Immunol (1985) 0.84
Changes in B lineage cell population in liver and spleen of normal neonatal mice. J Immunol (1983) 0.81
Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science (1997) 15.48
Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. Cell (1996) 13.95
Identification of ubiquitin ligases required for skeletal muscle atrophy. Science (2001) 12.98
Efficient in vivo manipulation of mouse genomic sequences at the zygote stage. Proc Natl Acad Sci U S A (1996) 12.92
Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo. Nat Cell Biol (2001) 10.85
ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF. Cell (1991) 9.54
Vessel cooption, regression, and growth in tumors mediated by angiopoietins and VEGF. Science (1999) 9.18
Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells. J Biol Chem (1978) 9.13
Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. Cell (1996) 9.10
An early haematopoietic defect in mice lacking the transcription factor GATA-2. Nature (1994) 8.60
Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions. Proc Natl Acad Sci U S A (1982) 8.53
Leakage-resistant blood vessels in mice transgenically overexpressing angiopoietin-1. Science (1999) 6.96
Interleukin-2 receptor alpha chain regulates the size and content of the peripheral lymphoid compartment. Immunity (1995) 6.91
Mediation of IGF-1-induced skeletal myotube hypertrophy by PI(3)K/Akt/mTOR and PI(3)K/Akt/GSK3 pathways. Nat Cell Biol (2001) 6.71
Ordered rearrangement of immunoglobulin heavy chain variable region segments. EMBO J (1984) 6.67
Essential role for oncogenic Ras in tumour maintenance. Nature (1999) 6.51
Developmentally controlled and tissue-specific expression of unrearranged VH gene segments. Cell (1985) 6.21
Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell lines. Nature (1984) 5.75
Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3' ends. Cell (1980) 5.59
Angiopoietin-1 protects the adult vasculature against plasma leakage. Nat Med (2000) 5.58
Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell (1995) 5.58
Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science (1994) 4.99
A functional T3 molecule associated with a novel heterodimer on the surface of immature human thymocytes. Nature (1986) 4.95
Choice of STATs and other substrates specified by modular tyrosine-based motifs in cytokine receptors. Science (1995) 4.90
A critical role for DNA end-joining proteins in both lymphogenesis and neurogenesis. Cell (1998) 4.78
Impaired B cell development and proliferation in absence of phosphoinositide 3-kinase p85alpha. Science (1999) 4.77
Interplay of p53 and DNA-repair protein XRCC4 in tumorigenesis, genomic stability and development. Nature (2000) 4.75
Impairment of V(D)J recombination in double-strand break repair mutants. Science (1993) 4.72
An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control. Science (1990) 4.63
Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV. Nature (1998) 4.37
The T cell leukemia oncoprotein SCL/tal-1 is essential for development of all hematopoietic lineages. Cell (1996) 4.35
The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination. Cell (1995) 4.35
Delta-like ligand 4 (Dll4) is induced by VEGF as a negative regulator of angiogenic sprouting. Proc Natl Acad Sci U S A (2007) 4.27
Regulation of gliogenesis in the central nervous system by the JAK-STAT signaling pathway. Science (1997) 4.26
Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines. Mol Cell Biol (1982) 4.26
Gene amplification and drug resistance in cultured murine cells. Science (1978) 4.25
Defective B cell development and function in Btk-deficient mice. Immunity (1995) 4.20
Development of the primary antibody repertoire. Science (1987) 4.19
Insertion of N regions into heavy-chain genes is correlated with expression of terminal deoxytransferase in B cells. Nature (1984) 4.18
Transposition and amplification of oncogene-related sequences in human neuroblastomas. Cell (1983) 4.13
Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and inability to support V(D)J recombination. Proc Natl Acad Sci U S A (1997) 4.13
Eph receptors and ligands comprise two major specificity subclasses and are reciprocally compartmentalized during embryogenesis. Neuron (1996) 4.12
Differentiation stage-specific inhibition of the Raf-MEK-ERK pathway by Akt. Science (1999) 4.10
Regulation of the assembly and expression of variable-region genes. Annu Rev Immunol (1986) 4.09
Analysis of C-MYC function in normal cells via conditional gene-targeted mutation. Immunity (2001) 4.09
Binding of myc proteins to canonical and noncanonical DNA sequences. Mol Cell Biol (1993) 4.08
DNA ligase IV deficiency in mice leads to defective neurogenesis and embryonic lethality via the p53 pathway. Mol Cell (2000) 3.82
The receptor tyrosine kinase MuSK is required for neuromuscular junction formation in vivo. Cell (1996) 3.81
Tie2 receptor ligands, angiopoietin-1 and angiopoietin-2, modulate VEGF-induced postnatal neovascularization. Circ Res (1998) 3.79
Recombination between immunoglobulin variable region gene segments is enhanced by transcription. Nature (1987) 3.74
Immunoglobulin gene rearrangement in B cell deficient mice generated by targeted deletion of the JH locus. Int Immunol (1993) 3.67
Increased vascularization in mice overexpressing angiopoietin-1. Science (1998) 3.67
Regulation of genome rearrangement events during lymphocyte differentiation. Immunol Rev (1986) 3.65
RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development. Proc Natl Acad Sci U S A (1993) 3.65
N-myc can functionally replace c-myc in murine development, cellular growth, and differentiation. Genes Dev (2000) 3.62
A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination. Immunity (1998) 3.58
Differential expression of myc family genes during murine development. Nature (1986) 3.57
The CBFbeta subunit is essential for CBFalpha2 (AML1) function in vivo. Cell (1996) 3.55
Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity. Science (1994) 3.53
Introduced T cell receptor variable region gene segments recombine in pre-B cells: evidence that B and T cells use a common recombinase. Cell (1986) 3.51
New model of tumor angiogenesis: dynamic balance between vessel regression and growth mediated by angiopoietins and VEGF. Oncogene (1999) 3.45
Human N-myc is closely related in organization and nucleotide sequence to c-myc. Nature (1986) 3.40
Ku70 is required for late B cell development and immunoglobulin heavy chain class switching. J Exp Med (1998) 3.37
Growth factors acting via endothelial cell-specific receptor tyrosine kinases: VEGFs, angiopoietins, and ephrins in vascular development. Genes Dev (1999) 3.35
Growth retardation and leaky SCID phenotype of Ku70-deficient mice. Immunity (1997) 3.35
Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation. Proc Natl Acad Sci U S A (1991) 3.31
The nonhomologous end-joining pathway of DNA repair is required for genomic stability and the suppression of translocations. Proc Natl Acad Sci U S A (2000) 3.21
NT-3, BDNF, and NGF in the developing rat nervous system: parallel as well as reciprocal patterns of expression. Neuron (1990) 3.12
PDZ proteins bind, cluster, and synaptically colocalize with Eph receptors and their ephrin ligands. Neuron (1998) 3.09
VEGF-initiated blood-retinal barrier breakdown in early diabetes. Invest Ophthalmol Vis Sci (2001) 3.08
Immunoglobulin heavy-chain expression and class switching in a murine leukaemia cell line. Nature (1982) 3.05
Restoration of T cell development in RAG-2-deficient mice by functional TCR transgenes. Science (1993) 2.93
LIFR beta and gp130 as heterodimerizing signal transducers of the tripartite CNTF receptor. Science (1993) 2.93
Wiskott-Aldrich syndrome protein-deficient mice reveal a role for WASP in T but not B cell activation. Immunity (1998) 2.92
N-WASP deficiency reveals distinct pathways for cell surface projections and microbial actin-based motility. Nat Cell Biol (2001) 2.91
Neurotrophin-3: a neurotrophic factor related to NGF and BDNF. Science (1990) 2.91
An orphan receptor tyrosine kinase family whose members serve as nonintegrin collagen receptors. Mol Cell (1997) 2.89
Novel immunoglobulin heavy chains are produced from DJH gene segment rearrangements in lymphoid cells. Nature (1985) 2.85
Bidirectional signalling through the EPH-family receptor Nuk and its transmembrane ligands. Nature (1996) 2.81
Agrin acts via a MuSK receptor complex. Cell (1996) 2.81
Lack of N regions in antigen receptor variable region genes of TdT-deficient lymphocytes. Science (1993) 2.80
Multiple growth factors, cytokines, and neurotrophins rescue photoreceptors from the damaging effects of constant light. Proc Natl Acad Sci U S A (1992) 2.78
Mitogen- and IL-4-regulated expression of germ-line Ig gamma 2b transcripts: evidence for directed heavy chain class switching. Cell (1988) 2.77
A BDNF autocrine loop in adult sensory neurons prevents cell death. Nature (1995) 2.75
VDJ recombination. Immunol Today (1992) 2.74
Embryonic lethality in mice homozygous for a targeted disruption of the N-myc gene. Genes Dev (1992) 2.72
The scid defect affects the final step of the immunoglobulin VDJ recombinase mechanism. Cell (1988) 2.71
BDNF is a neurotrophic factor for dopaminergic neurons of the substantia nigra. Nature (1991) 2.70
Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice. Science (1993) 2.68
Activity of multiple light chain genes in murine myeloma cells producing a single, functional light chain. Cell (1980) 2.68
Secondary genomic rearrangement events in pre-B cells: VHDJH replacement by a LINE-1 sequence and directed class switching. EMBO J (1986) 2.62