Published in Cell on December 01, 1979
Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J (1982) 17.98
Gene structure prediction and alternative splicing analysis using genomically aligned ESTs. Genome Res (2001) 5.38
Introns increase transcriptional efficiency in transgenic mice. Proc Natl Acad Sci U S A (1988) 5.18
Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol Cell Biol (1981) 5.00
Structure and expression of the human gene encoding major heat shock protein HSP70. Mol Cell Biol (1985) 4.79
Comparison of intron-dependent and intron-independent gene expression. Mol Cell Biol (1988) 4.75
Splicing is required for rapid and efficient mRNA export in metazoans. Proc Natl Acad Sci U S A (1999) 4.26
Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector. Proc Natl Acad Sci U S A (1981) 3.38
Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA. Nucleic Acids Res (1990) 3.34
Unusual alpha-globin-like gene that has cleanly lost both globin intervening sequences. Proc Natl Acad Sci U S A (1980) 3.19
Herpes simplex virus type 1 HindIII fragment L encodes spliced and complementary mRNA species. J Virol (1981) 2.97
Effect of intron A from human cytomegalovirus (Towne) immediate-early gene on heterologous expression in mammalian cells. Nucleic Acids Res (1991) 2.92
Expression of simian virus 40-rat preproinsulin recombinants in monkey kidney cells: use of preproinsulin RNA processing signals. Proc Natl Acad Sci U S A (1981) 2.76
A generic intron increases gene expression in transgenic mice. Mol Cell Biol (1991) 2.75
The leader sequence of the subgenomic mRNA's of Rous sarcoma virus is approximately 390 nucleotides. J Virol (1982) 2.51
Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells. Proc Natl Acad Sci U S A (1982) 2.45
Transcripts of the adeno-associated virus genome: mapping of the major RNAs. J Virol (1980) 2.42
Importance of introns for expression of mouse ribosomal protein gene rpL32. Mol Cell Biol (1989) 2.40
Construction of a modular dihydrofolate reductase cDNA gene: analysis of signals utilized for efficient expression. Mol Cell Biol (1982) 2.33
Distinct RNP complexes of shuttling hnRNP proteins with pre-mRNA and mRNA: candidate intermediates in formation and export of mRNA. Mol Cell Biol (2001) 2.32
Control of expression of histocompatibility antigens (H-2) and beta 2-microglobulin in F9 teratocarcinoma stem cells. Proc Natl Acad Sci U S A (1981) 2.22
A novel hepatitis B virus (HBV) genetic element with Rev response element-like properties that is essential for expression of HBV gene products. Mol Cell Biol (1993) 2.12
Construction and functional characterization of polyomavirus genomes that separately encode the three early proteins. J Virol (1984) 2.02
The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts. EMBO J (1985) 1.99
Splicing promotes rapid and efficient mRNA export in mammalian cells. Proc Natl Acad Sci U S A (2008) 1.98
A nucleotide change at a splice junction in the human beta-globin gene is associated with beta 0-thalassemia. Proc Natl Acad Sci U S A (1981) 1.93
Intervening sequences in a Dictyostelium gene that encodes a low abundance class mRNA. Nucleic Acids Res (1980) 1.82
Chicken ovalbumin gene fused to a herpes simplex virus alpha promoter and linked to a thymidine kinase gene is regulated like a viral gene. Mol Cell Biol (1982) 1.73
Intronless mRNA transport elements may affect multiple steps of pre-mRNA processing. EMBO J (1999) 1.71
The absence of introns within a human fibroblast interferon gene. Nucleic Acids Res (1981) 1.63
The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing. Mol Cell Biol (1990) 1.62
The intron requirement for immunoglobulin gene expression is dependent upon the promoter. Nucleic Acids Res (1988) 1.60
Expression of complete chicken thymidine kinase gene inserted in a retrovirus vector. Mol Cell Biol (1984) 1.58
Gene expression from both intronless and intron-containing Rous sarcoma virus clones is specifically inhibited by anti-sense RNA. Mol Cell Biol (1985) 1.58
Increased gene expression by the first intron of maize shrunken-1 locus in grass species. Plant Physiol (1989) 1.54
New chimeric splice junction in adenovirus type 2-simian virus 40 hybrid viral mRNA. J Virol (1980) 1.53
Effect of intron size on splicing efficiency in retroviral transcripts. Nucleic Acids Res (1982) 1.46
Reiterated sequences within the intron of an immediate-early gene of herpes simplex virus type 1. Nucleic Acids Res (1981) 1.42
The sequence complementarity between HIV-1 5' splice site SD4 and U1 snRNA determines the steady-state level of an unstable env pre-mRNA. RNA (2001) 1.42
Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells. Mol Cell Biol (1987) 1.41
T-antigen expression by polyoma mutants with modified RNA splicing. EMBO J (1983) 1.39
Electroporation: application to human lymphoid cell lines for stable introduction of a transactivator gene of human T-cell leukemia virus type I. Nucleic Acids Res (1989) 1.38
Efficient 3'-end formation of human beta-globin mRNA in vivo requires sequences within the last intron but occurs independently of the splicing reaction. Nucleic Acids Res (1998) 1.38
Mutation in an intervening sequence splice junction in man. Proc Natl Acad Sci U S A (1981) 1.34
Isolation and characterization of the RNA2+, RNA4+, and RNA11+ genes of Saccharomyces cerevisiae. J Bacteriol (1984) 1.31
Abundant nuclear ribonucleoprotein form of CAD RNA. Mol Cell Biol (1985) 1.30
Thymidylate synthase gene expression is stimulated by some (but not all) introns. Nucleic Acids Res (1989) 1.28
A cis-acting repressive sequence that overlaps the Rev-responsive element of human immunodeficiency virus type 1 regulates nuclear retention of env mRNAs independently of known splice signals. Proc Natl Acad Sci U S A (1994) 1.26
In vitro splicing of influenza viral NS1 mRNA and NS1-beta-globin chimeras: possible mechanisms for the control of viral mRNA splicing. Proc Natl Acad Sci U S A (1986) 1.25
Synthesis of predominantly unspliced cytoplasmic RNAs by chimeric herpes simplex virus type 1 thymidine kinase-human beta-globin genes. Mol Cell Biol (1985) 1.24
Structure and expression of the Chinese hamster thymidine kinase gene. Mol Cell Biol (1986) 1.24
The length but not the sequence of the polyoma virus late leader exon is important for both late RNA splicing and stability. Nucleic Acids Res (1987) 1.21
Cis and trans-acting elements involved in the activation of Arabidopsis thaliana A1 gene encoding the translation elongation factor EF-1 alpha. Nucleic Acids Res (1991) 1.18
Synthesis of stable unspliced mRNA from an intronless simian virus 40--rat preproinsulin gene recombinant. Proc Natl Acad Sci U S A (1981) 1.18
Induction of premature termination of transcription of the mouse beta-globin gene by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB). Nucleic Acids Res (1981) 1.17
A splice junction deletion deficient in the transport of RNA does not polyadenylate nuclear RNA. Mol Cell Biol (1983) 1.16
The 5'-flanking region of the mouse thymidylate synthase gene is necessary but not sufficient for normal regulation in growth-stimulated cells. Mol Cell Biol (1991) 1.11
Importance of introns in the growth regulation of mRNA levels of the proliferating cell nuclear antigen gene. Mol Cell Biol (1990) 1.08
Construction of novel cytochrome b genes in yeast mitochondria by subtraction or addition of introns. EMBO J (1983) 1.07
Herpes simplex virus ICP27 induces cytoplasmic accumulation of unspliced polyadenylated alpha-globin pre-mRNA in infected HeLa cells. J Virol (2000) 1.06
In vitro-synthesized adenovirus 2 messenger RNA precursors are accurately spliced by nuclear extracts. Proc Natl Acad Sci U S A (1984) 1.05
Processing of alpha-globin and ICP0 mRNA in cells infected with herpes simplex virus type 1 ICP27 mutants. J Virol (2000) 1.04
Stimulation of gene expression by introns: conversion of an inhibitory intron to a stimulatory intron by alteration of the splice donor sequence. Nucleic Acids Res (1993) 1.03
Simian virus 40 mutant with transposed T-antigen and VP1 genes. J Virol (1982) 1.02
Secondary structure model for the complete simian virus 50 late precursor mRNA. Nucleic Acids Res (1982) 0.99
Expression of human alpha-globin and mouse/human hybrid beta-globin genes in murine hemopoietic stem cells transduced by recombinant retroviruses. Proc Natl Acad Sci U S A (1990) 0.93
5' Nucleotide sequences influence serum-modulated expression of a human dihydrofolate reductase minigene. Mol Cell Biol (1986) 0.92
Elongation and maturation of c-myc RNA is inhibited by differentiation inducing agents in HL60 cells. Nucleic Acids Res (1990) 0.91
Gene transfer from targeted liposomes to specific lymphoid cells by electroporation. Proc Natl Acad Sci U S A (1988) 0.91
Targeted nanoparticles enhanced flow electroporation of antisense oligonucleotides in leukemia cells. Biosens Bioelectron (2010) 0.90
Genetic test for involvement of intervening sequences in transport of nuclear RNA. Mol Cell Biol (1982) 0.89
Splicing signals are required for S-phase regulation of the mouse thymidylate synthase gene. Mol Cell Biol (1996) 0.89
Split-intron retroviral vectors: enhanced expression with improved safety. J Virol (2000) 0.87
Effect of transfection strategy on growth factor overexpression by articular chondrocytes. J Orthop Res (2010) 0.86
The sequence and context of the 5' splice site govern the nuclear stability of polyoma virus late RNAs. Nucleic Acids Res (1995) 0.84
Gold nanoparticles enhanced electroporation for mammalian cell transfection. J Biomed Nanotechnol (2014) 0.84
Efficient production of chimeric mice from embryonic stem cells injected into 4- to 8-cell and blastocyst embryos. J Anim Sci Biotechnol (2013) 0.83
Retroviral vectors produced in the cytoplasmic vaccinia virus system transduce intron-containing genes. J Virol (2002) 0.83
Spliced and unspliced virus specific RNA sequences are associated with purified simian virus 40 chromatin. Nucleic Acids Res (1982) 0.80
Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters. Photochem Photobiol Sci (2010) 0.78
Expression of Escherichia coli branching enzyme in caryopses of transgenic rice results in amylopectin with an increased degree of branching. Planta (2004) 0.78
Transcription of a cloned rainbow trout protamine gene is accurately initiated following transfection into HeLa cells but the majority of the transcripts fail to polyadenylate at the correct site. Nucleic Acids Res (1985) 0.77
Polymorphism rs2239185 in vitamin D receptor gene is associated with severe community-acquired pneumonia of children in Chinese Han population: a case-control study. Eur J Pediatr (2014) 0.76
Size Specific Transfection to Mammalian Cells by Micropillar Array Electroporation. Sci Rep (2016) 0.75
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A (1972) 83.88
Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell (1983) 17.87
Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc Natl Acad Sci U S A (1984) 17.51
Association of anxiety-related traits with a polymorphism in the serotonin transporter gene regulatory region. Science (1996) 16.04
EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system. Science (1977) 13.24
Mice lacking p21CIP1/WAF1 undergo normal development, but are defective in G1 checkpoint control. Cell (1995) 12.39
Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc Natl Acad Sci U S A (1982) 10.50
The sequence of the chromosomal mouse beta-globin major gene: homologies in capping, splicing and poly(A) sites. Cell (1978) 9.73
Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene. Proc Natl Acad Sci U S A (1979) 9.31
Structure of the human immunoglobulin mu locus: characterization of embryonic and rearranged J and D genes. Cell (1981) 9.09
Cell surface, heparin-like molecules are required for binding of basic fibroblast growth factor to its high affinity receptor. Cell (1991) 8.99
Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell (1980) 8.77
The human c-myc oncogene: structural consequences of translocation into the IgH locus in Burkitt lymphoma. Cell (1983) 8.54
Metallothionein. Annu Rev Biochem (1986) 8.12
The evolution and sequence comparison of two recently diverged mouse chromosomal beta--globin genes. Cell (1979) 8.11
In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer). Proc Natl Acad Sci U S A (1972) 7.89
Fibroblast growth factor receptor 3 is a negative regulator of bone growth. Cell (1996) 7.70
Single-step induction of mammary adenocarcinoma in transgenic mice bearing the activated c-neu oncogene. Cell (1988) 7.50
The death domain kinase RIP mediates the TNF-induced NF-kappaB signal. Immunity (1998) 7.35
The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes. J Biol Chem (1981) 7.26
Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes. Cell (1984) 6.71
A kappa-immunoglobulin gene is formed by site-specific recombination without further somatic mutation. Nature (1979) 6.54
Protein synthesis directed by encephalomyocarditis virus RNA: properties of a transfer RNA-dependent system. Proc Natl Acad Sci U S A (1971) 6.52
c-Jun dimerizes with itself and with c-Fos, forming complexes of different DNA binding affinities. Cell (1988) 6.24
Translocations among antibody genes in human cancer. Science (1983) 6.20
The intervening sequence of a mouse beta-globin gene is transcribed within the 15S beta-globin mRNA precursor. Proc Natl Acad Sci U S A (1978) 6.20
The complete sequence of a chromosomal mouse alpha--globin gene reveals elements conserved throughout vertebrate evolution. Cell (1979) 6.05
RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell (1995) 5.73
Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt lymphoma. Cell (1984) 5.61
Protein synthesis directed by encephalomyocarditis virus RNA. II. The in vitro synthesis of high molecular weight proteins and elements of the viral capsid. Biochem Biophys Res Commun (1971) 5.60
Organization and complete sequence of identical embryonic and plasmacytoma kappa V-region genes. J Biol Chem (1980) 5.59
Coexpression of MMTV/v-Ha-ras and MMTV/c-myc genes in transgenic mice: synergistic action of oncogenes in vivo. Cell (1987) 5.57
Purification and properties of biologically active messenger RNA for a myeloma light chain. Proc Natl Acad Sci U S A (1972) 5.28
Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences. Proc Natl Acad Sci U S A (1977) 5.28
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Antibody diversity. Science (1978) 5.06
The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus. Cell (1990) 4.91
The arrangement and rearrangement of antibody genes. Nature (1979) 4.88
Clustered arrangement of immunoglobulin lambda constant region genes in man. Nature (1981) 4.87
Functional role for c-myc in mitogenic response to platelet-derived growth factor. Nature (1984) 4.56
The kit ligand: a cell surface molecule altered in steel mutant fibroblasts. Cell (1990) 4.43
Butyric acid, a potent inducer of erythroid differentiation in cultured erythroleukemic cells. Cell (1975) 4.32
Processed genes: a dispersed human immunoglobulin gene bearing evidence of RNA-type processing. Nature (1982) 4.14
Human immunoglobulin heavy chain genes map to a region of translocations in malignant B lymphocytes. Science (1982) 4.05
Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells. Proc Natl Acad Sci U S A (1972) 4.02
Intervening sequence of DNA identified in the structural portion of a mouse beta-globin gene. Proc Natl Acad Sci U S A (1978) 4.02
Murine FGFR-1 is required for early postimplantation growth and axial organization. Genes Dev (1994) 3.97
Daptomycin-resistant, methicillin-resistant Staphylococcus aureus bacteremia. Clin Infect Dis (2005) 3.90
Multiple related immunoglobulin variable-region genes identified by cloning and sequence analysis. Proc Natl Acad Sci U S A (1978) 3.88
Purification and cloning of a mouse ribosomal gene fragment in coliphage lambda. Gene (1977) 3.79
An eosinophil-dependent mechanism for the antitumor effect of interleukin-4. Science (1992) 3.78
Pleiotropic defects in ataxia-telangiectasia protein-deficient mice. Proc Natl Acad Sci U S A (1996) 3.78
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Consequences of widespread deregulation of the c-myc gene in transgenic mice: multiple neoplasms and normal development. Cell (1986) 3.70
Activation and somatic mutation of the translocated c-myc gene in burkitt lymphoma cells. Cell (1984) 3.66
Human immunoglobulin kappa light-chain genes are deleted or rearranged in lambda-producing B cells. Nature (1981) 3.64
Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation. Science (1984) 3.63
Translation of artemether-lumefantrine treatment policy into paediatric clinical practice: an early experience from Kenya. Trop Med Int Health (2008) 3.62
Transmembrane form of the kit ligand growth factor is determined by alternative splicing and is missing in the Sld mutant. Cell (1991) 3.60
Murine interleukin-4 displays potent anti-tumor activity in vivo. Cell (1989) 3.43
High capacity gel preparative electrophoresis for purification of fragments of genomic DNA. Anal Biochem (1978) 3.42
Evolution of human immunoglobulin kappa J region genes. J Biol Chem (1982) 3.41
Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells. Mol Cell Biol (1992) 3.41
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Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector. Proc Natl Acad Sci U S A (1981) 3.38
Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI. Proc Natl Acad Sci U S A (1976) 3.32
Nuclear localization and DNA binding properties of a protein expressed by human c-myc oncogene. Science (1984) 3.24
Unusual alpha-globin-like gene that has cleanly lost both globin intervening sequences. Proc Natl Acad Sci U S A (1980) 3.19
Regulation of a metallothionein-growth hormone hybrid gene in bovine papilloma virus. Proc Natl Acad Sci U S A (1983) 3.06
An inherited limb deformity created by insertional mutagenesis in a transgenic mouse. Nature (1985) 3.05
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Human immunoglobulin D segments encoded in tandem multigenic families. Nature (1981) 2.99
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IL-4 induces allergic-like inflammatory disease and alters T cell development in transgenic mice. Cell (1990) 2.86
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Parental-specific methylation of an imprinted transgene is established during gametogenesis and progressively changes during embryogenesis. Cell (1991) 2.85
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Duplicated heavy metal control sequences of the mouse metallothionein-I gene. Proc Natl Acad Sci U S A (1984) 2.69
The IP-10 chemokine binds to a specific cell surface heparan sulfate site shared with platelet factor 4 and inhibits endothelial cell proliferation. J Exp Med (1995) 2.68
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Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia. Nat Med (1996) 2.55
Dispersion of alpha-like globin genes of the mouse to three different chromosomes. Nature (1981) 2.44
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Safer derivatives of bacteriophage lambdagt-lambdaC for use in cloning of recombinant DNA molecules. Nature (1976) 2.35
IP-10, a -C-X-C- chemokine, elicits a potent thymus-dependent antitumor response in vivo. J Exp Med (1993) 2.35
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Expression of the chromosomal mouse Beta maj-globin gene cloned in SV40. Nature (1979) 2.29