Published in Proc Natl Acad Sci U S A on February 01, 1977
DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A (1977) 790.54
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A (1980) 109.70
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res (1984) 86.56
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A (1979) 72.18
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A (1983) 54.64
A system for shotgun DNA sequencing. Nucleic Acids Res (1981) 52.99
Enhanced graphic matrix analysis of nucleic acid and protein sequences. Proc Natl Acad Sci U S A (1981) 46.11
Sequence data handling by computer. Nucleic Acids Res (1977) 45.33
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res (1981) 35.39
Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A (1978) 32.65
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates. Nucleic Acids Res (1979) 31.23
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity. Nucleic Acids Res (1978) 30.43
Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res (1977) 26.63
Direct chemical method for sequencing RNA. Proc Natl Acad Sci U S A (1979) 25.15
A strategy of DNA sequencing employing computer programs. Nucleic Acids Res (1979) 19.82
The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J (1986) 19.59
Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A (1978) 19.34
GEL, a DNA sequencing project management system. Nucleic Acids Res (1982) 18.85
The ovalbumin gene-sequence of putative control regions. Nucleic Acids Res (1980) 18.04
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo. Proc Natl Acad Sci U S A (1980) 16.88
Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries. Proc Natl Acad Sci U S A (1978) 14.82
Computer programs for the assembly of DNA sequences. Nucleic Acids Res (1979) 13.36
pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res (1978) 13.22
Nucleotide sequence of an insertion element, IS1. Proc Natl Acad Sci U S A (1978) 11.43
Isolation and sequence of the gene for actin in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1980) 11.24
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci U S A (1980) 11.09
Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family. Nucleic Acids Res (1985) 10.76
Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o. Proc Natl Acad Sci U S A (1977) 10.64
A bacterial clone synthesizing proinsulin. Proc Natl Acad Sci U S A (1978) 10.23
The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene. Nucleic Acids Res (1980) 9.96
Light-generated oligonucleotide arrays for rapid DNA sequence analysis. Proc Natl Acad Sci U S A (1994) 9.59
The 65-kilodalton antigen of Mycobacterium tuberculosis. J Bacteriol (1987) 9.50
Ubiquitous, interspersed repeated sequences in mammalian genomes. Proc Natl Acad Sci U S A (1980) 9.48
Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1981) 9.45
Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7. Proc Natl Acad Sci U S A (1980) 9.32
Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene. Proc Natl Acad Sci U S A (1979) 9.31
Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli. Proc Natl Acad Sci U S A (1979) 9.28
A nuclear factor for IL-6 expression (NF-IL6) is a member of a C/EBP family. EMBO J (1990) 9.17
Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies. J Bacteriol (1982) 9.02
Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1980) 8.88
Structure-independent nucleotide sequence analysis. Proc Natl Acad Sci U S A (1979) 8.65
A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes. Proc Natl Acad Sci U S A (1982) 8.15
Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Res (1979) 8.00
The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae. Nucleic Acids Res (1980) 7.85
Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A (1980) 7.80
Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions. Proc Natl Acad Sci U S A (1980) 7.77
Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression. Proc Natl Acad Sci U S A (1980) 7.77
Probing the structure of RNAs in solution. Nucleic Acids Res (1987) 7.62
Restriction maps for twenty-one Charon vector phages. J Virol (1980) 7.51
The nucleotide sequence of the rat cytoplasmic beta-actin gene. Nucleic Acids Res (1983) 7.47
Specific 5' flanking sequences are required for faithful initiation of in vitro transcription of the ovalbumin gene. Proc Natl Acad Sci U S A (1981) 7.40
Nucleotide sequence of bacteriophage fd DNA. Nucleic Acids Res (1978) 7.25
Secondary structure model for 23S ribosomal RNA. Nucleic Acids Res (1981) 7.12
Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor. J Exp Med (1988) 7.12
Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication. Proc Natl Acad Sci U S A (1980) 7.04
Detection of 5-methylcytosine in DNA sequences. Nucleic Acids Res (1978) 7.03
SEQAID: a DNA sequence assembling program based on a mathematical model. Nucleic Acids Res (1984) 6.91
Sequences of the recA gene and protein. Proc Natl Acad Sci U S A (1980) 6.87
The inverted repeat as a recognizable structural feature in supercoiled DNA molecules. Proc Natl Acad Sci U S A (1980) 6.83
Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain. Proc Natl Acad Sci U S A (1978) 6.80
Bovine papillomavirus contains multiple transforming genes. Proc Natl Acad Sci U S A (1985) 6.73
Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli. J Virol (1984) 6.73
Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment: implications for retrovirus integration. Proc Natl Acad Sci U S A (1980) 6.73
The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA. Nucleic Acids Res (1981) 6.69
Viral integration and excision: structure of the lambda att sites. Science (1977) 6.66
Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J Virol (1987) 6.65
Specificity of Hpa II and Hae III DNA methylases. Nucleic Acids Res (1977) 6.62
Activities of human immunodeficiency virus (HIV) integration protein in vitro: specific cleavage and integration of HIV DNA. Proc Natl Acad Sci U S A (1991) 6.58
End labeling of enzymatically decapped mRNA. Nucleic Acids Res (1977) 6.58
Tissue-specific and light-regulated expression of a pea nuclear gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase. EMBO J (1984) 6.53
A method for the recovery of DNA from agarose gels. Nucleic Acids Res (1978) 6.24
Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol (1984) 6.23
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus. EMBO J (1989) 6.19
The interaction of RNA polymerase and lac repressor with the lac control region. Nucleic Acids Res (1979) 6.17
Yeast recombination: the association between double-strand gap repair and crossing-over. Proc Natl Acad Sci U S A (1983) 6.10
Hepatitis C virus (HCV) circulates as a population of different but closely related genomes: quasispecies nature of HCV genome distribution. J Virol (1992) 6.09
Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer. Proc Natl Acad Sci U S A (1981) 6.03
Simian virus 40 early mRNA's. I. Genomic localization of 3' and 5' termini and two major splices in mRNA from transformed and lytically infected cells. J Virol (1979) 5.93
Analysis of the nucleotide sequence of an invertible controlling element. Proc Natl Acad Sci U S A (1980) 5.92
"Nonrandom" DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method. Proc Natl Acad Sci U S A (1982) 5.90
Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol (1989) 5.85
In vitro mutagenesis of the promoter region for a vaccinia virus gene: evidence for tandem early and late regulatory signals. J Virol (1985) 5.77
Isolation and localization of DNA segments from specific human chromosomes. Proc Natl Acad Sci U S A (1980) 5.76
Cloning of adeno-associated virus into pBR322: rescue of intact virus from the recombinant plasmid in human cells. Proc Natl Acad Sci U S A (1982) 5.73
The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing. Nucleic Acids Res (1981) 5.70
Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins. Proc Natl Acad Sci U S A (1981) 5.57
Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc Natl Acad Sci U S A (1986) 5.54
Intragenic cis-acting art gene-responsive sequences of the human immunodeficiency virus. Proc Natl Acad Sci U S A (1988) 5.47
Nucleotide sequence of the bacterial transposon Tn1681 encoding a heat-stable (ST) toxin and its identification in enterotoxigenic Escherichia coli strains. Proc Natl Acad Sci U S A (1980) 5.46
Four small Drosophila heat shock proteins are related to each other and to mammalian alpha-crystallin. Proc Natl Acad Sci U S A (1982) 5.39
Chemical probes for higher-order structure in RNA. Proc Natl Acad Sci U S A (1980) 5.38
Origin of replication of colicin E1 plasmid DNA. Proc Natl Acad Sci U S A (1977) 5.38
Expression of early genes of origin-defective mutants of simian virus 40. Proc Natl Acad Sci U S A (1980) 5.33
In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites. Proc Natl Acad Sci U S A (1978) 5.32
Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome. Proc Natl Acad Sci U S A (1979) 5.31
Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes. Nucleic Acids Res (1986) 5.29
Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA. Proc Natl Acad Sci U S A (1978) 5.29
DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus. Proc Natl Acad Sci U S A (1981) 5.26
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J (1964) 41.13
A specific endonuclease from Arthrobacter luteus. J Mol Biol (1976) 25.89
FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDES. Biochem J (1963) 25.31
Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res (1976) 23.88
Gel electrophoretic separation of the complementary strands of bacteriophage DNA. Virology (1972) 23.10
Effect of salts and polyamines on T4 polynucleotide kinase. Biochemistry (1975) 22.01
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Reversal of bacteriophage T4 induced polynucleotide kinase action. Biochemistry (1973) 21.41
Hydrazinolysis of some purines and pyrimidines and their related nucleosides and nucleotides. J Chem Soc Perkin 1 (1967) 21.11
The degradation of DNA by hydrazine: a critical study of the suitability of the reaction for the quantitative determination of purine nucleotide sequences. Biochim Biophys Acta (1969) 20.65
METHYLATION OF DEOXYRIBONUCLEIC ACID BY DIAZOMETHANE. Biochim Biophys Acta (1964) 20.40
Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol (1980) 287.68
Genomic sequencing. Proc Natl Acad Sci U S A (1984) 92.36
Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res (1977) 26.63
E. coli RNA polymerase interacts homologously with two different promoters. Cell (1980) 19.71
Why genes in pieces? Nature (1978) 19.03
Nucleotide sequence of Rous sarcoma virus. Cell (1983) 17.80
Immunological screening method to detect specific translation products. Proc Natl Acad Sci U S A (1978) 17.80
Molecular basis of base substitution hotspots in Escherichia coli. Nature (1978) 13.79
Construction of plasmids carrying the cI gene of bacteriophage lambda. Proc Natl Acad Sci U S A (1976) 13.10
The nucleotide sequence of the lac operator. Proc Natl Acad Sci U S A (1973) 11.28
Hepatitis B virus genes and their expression in E. coli. Nature (1979) 10.83
The evolution of genes: the chicken preproinsulin gene. Cell (1980) 10.25
A bacterial clone synthesizing proinsulin. Proc Natl Acad Sci U S A (1978) 10.23
B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo. Science (1985) 9.73
Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7. Proc Natl Acad Sci U S A (1980) 9.32
The structure and evolution of the two nonallelic rat preproinsulin genes. Cell (1979) 9.25
Formation of parallel four-stranded complexes by guanine-rich motifs in DNA and its implications for meiosis. Nature (1988) 8.58
DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol (1968) 8.37
Absence of polymorphism at the ZFY locus on the human Y chromosome. Science (1995) 7.86
Rous sarcoma virus genome is terminally redundant: the 5' sequence. Proc Natl Acad Sci U S A (1977) 7.09
Detection of 5-methylcytosine in DNA sequences. Nucleic Acids Res (1978) 7.03
Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain. Proc Natl Acad Sci U S A (1978) 6.80
Mutants that make more lac repressor. Proc Natl Acad Sci U S A (1968) 6.26
The genetic data environment an expandable GUI for multiple sequence analysis. Comput Appl Biosci (1994) 5.68
Chemical probes for higher-order structure in RNA. Proc Natl Acad Sci U S A (1980) 5.38
A sodium-potassium switch in the formation of four-stranded G4-DNA. Nature (1990) 5.35
Enzymatic in vitro synthesis of globin genes. Cell (1976) 5.31
The lac operator is DNA. Proc Natl Acad Sci U S A (1967) 5.24
Cloning, sequencing, and functional analysis of oriL, a herpes simplex virus type 1 origin of DNA synthesis. Mol Cell Biol (1985) 4.14
The lac repressor and the lac operator. Ciba Found Symp (1972) 4.01
An amino-terminal fragment of lac repressor binds specifically to lac operator. Proc Natl Acad Sci U S A (1978) 3.98
Cell-type-specific contacts to immunoglobulin enhancers in nuclei. Nature (1985) 3.94
MSI and MSII made on ribosome in idling step of protein synthesis. Nature (1972) 3.89
Structure mapping of 5'-32P-labeled RNA with S1 nuclease. Biochemistry (1978) 3.22
How big is the universe of exons? Science (1990) 3.16
On the antiquity of introns. Cell (1986) 2.98
Intron phase correlations and the evolution of the intron/exon structure of genes. Proc Natl Acad Sci U S A (1995) 2.98
The triosephosphate isomerase gene from maize: introns antedate the plant-animal divergence. Cell (1986) 2.95
Eukaryotic signal sequence transports insulin antigen in Escherichia coli. Proc Natl Acad Sci U S A (1980) 2.79
Genes-in-pieces revisited. Science (1985) 2.72
Towards a paradigm shift in biology. Nature (1991) 2.70
Structure of the genome of Moloney murine leukemia virus: a terminally redundant sequence. Cell (1978) 2.68
EID: the Exon-Intron Database-an exhaustive database of protein-coding intron-containing genes. Nucleic Acids Res (2000) 2.56
Contacts between the lac repressor and the thymines in the lac operator. Proc Natl Acad Sci U S A (1977) 2.52
Nobel laureates' letter to President Bush. Washington Post (2001) 2.43
Promotor region for yeast 5S ribosomal RNA. Nature (1977) 2.32
Rous sarcoma virus encodes a transcriptional activator. Cell (1985) 2.28
One-sided polymerase chain reaction: the amplification of cDNA. Proc Natl Acad Sci U S A (1989) 2.25
DNA-binding site of lac repressor probed by dimethylsulfate methylation of lac operator. J Mol Biol (1979) 2.20
Cellular location affects protein stability in Escherichia coli. Proc Natl Acad Sci U S A (1982) 2.20
Toward a resolution of the introns early/late debate: only phase zero introns are correlated with the structure of ancient proteins. Proc Natl Acad Sci U S A (1998) 2.16
Tissue-specific exposure of chromatin structure at the 5' terminus of the rat preproinsulin II gene. Proc Natl Acad Sci U S A (1981) 2.00
Somatically acquired recombinant murine leukemia proviruses in thymic leukemias of AKR/J mice. J Virol (1983) 1.99
Relationship between "proto-splice sites" and intron phases: evidence from dicodon analysis. Proc Natl Acad Sci U S A (1998) 1.90
Novel DNA superstructures formed by telomere-like oligomers. Biochemistry (1992) 1.82
Climate change and the integrity of science. Science (2010) 1.80
Primary structure of chicken muscle pyruvate kinase mRNA. Proc Natl Acad Sci U S A (1983) 1.79
The yeast KEM1 gene encodes a nuclease specific for G4 tetraplex DNA: implication of in vivo functions for this novel DNA structure. Cell (1994) 1.79
Sequences of controlling regions of the lactose operon. Cold Spring Harb Symp Quant Biol (1974) 1.76
Bacteria mature preproinsulin to proinsulin. Proc Natl Acad Sci U S A (1980) 1.75
Exploring the Mycoplasma capricolum genome: a minimal cell reveals its physiology. Mol Microbiol (1995) 1.74
Evolution of the intron-exon structure of eukaryotic genes. Curr Opin Genet Dev (1995) 1.72
Intron positions correlate with module boundaries in ancient proteins. Proc Natl Acad Sci U S A (1996) 1.66
Detection in vivo of protein-DNA interactions within the lac operon of Escherichia coli. Nature (1985) 1.63
Antibodies of the secondary response can be expressed without switch recombination in normal mouse B cells. Proc Natl Acad Sci U S A (1984) 1.57
Pleiotropic mutations within two yeast mitochondrial cytochrome genes block mRNA processing. Cell (1979) 1.51
An 'internal' signal sequence directs secretion and processing or proinsulin in bacteria. Nature (1981) 1.48
Early retinal vessel development and iris vessel dilatation as factors in retinopathy of prematurity. Cryotherapy for Retinopathy of Prematurity (CRYO-ROP) Cooperative Group. Arch Ophthalmol (1996) 1.44
Active site of triosephosphate isomerase: in vitro mutagenesis and characterization of an altered enzyme. Proc Natl Acad Sci U S A (1985) 1.44
Ligands for ErbB-family receptors encoded by a neuregulin-like gene. Nature (1997) 1.43
Identification and characterization of a nuclease activity specific for G4 tetrastranded DNA. Proc Natl Acad Sci U S A (1993) 1.43
Intron/exon structure of the chicken pyruvate kinase gene. Cell (1985) 1.40
Nucleotide sequence of the 3' half of AKV. Nucleic Acids Res (1982) 1.40
Retinoic acid is necessary for development of the ventral retina in zebrafish. Proc Natl Acad Sci U S A (1994) 1.39
Guanine quartet structures. Methods Enzymol (1992) 1.34
Location of the 5.8S rRNA gene of Saccharomyces cerevisiae. J Bacteriol (1978) 1.34
Large scale bacterial gene discovery by similarity search. Nat Genet (1994) 1.33
Dealing with database explosion: a cautionary note. Science (1997) 1.32
The T cell receptor V alpha 3 gene segment is associated with reactivity to p-azobenzenearsonate. Cell (1988) 1.31
Expression of the T-cell-specific gamma gene is unnecessary in T cells recognizing class II MHC determinants. Nature (1985) 1.29
Chicken triosephosphate isomerase complements an Escherichia coli deficiency. Proc Natl Acad Sci U S A (1985) 1.27
Genetic engineering in the Precambrian: structure of the chicken triosephosphate isomerase gene. Mol Cell Biol (1985) 1.22
Reaction energetics of a mutant triosephosphate isomerase in which the active-site glutamate has been changed to aspartate. Biochemistry (1986) 1.21
Introns and gene evolution. Genes Cells (1996) 1.19
Direct genomic sequencing of bacterial DNA: the pyruvate kinase I gene of Escherichia coli. Proc Natl Acad Sci U S A (1989) 1.17
Active T-cell receptor genes have intron deoxyribonuclease hypersensitive sites. Science (1985) 1.17
Production of immunologically active surface antigens of hepatitis B virus by Escherichia coli. Proc Natl Acad Sci U S A (1981) 1.16
Germ-line MuLV reintegrations in AKR/J mice. Nature (1982) 1.16
The genomic sequencing technique. Prog Clin Biol Res (1985) 1.15
A yeast gene product, G4p2, with a specific affinity for quadruplex nucleic acids. J Biol Chem (1995) 1.14
Exon shuffling and the origin of the mitochondrial targeting function in plant cytochrome c1 precursor. Proc Natl Acad Sci U S A (1996) 1.12
Gene disruption of a G4-DNA-dependent nuclease in yeast leads to cellular senescence and telomere shortening. Proc Natl Acad Sci U S A (1995) 1.11
The yeast splice site revisited: new exon consensus from genomic analysis. Cell (1997) 1.10
Isolation and mapping of cDNA hybridization probes specific for ecotropic and nonecotropic murine leukemia proviruses. Virology (1983) 1.09
Intron distribution difference for 276 ancient and 131 modern genes suggests the existence of ancient introns. Proc Natl Acad Sci U S A (2001) 1.07