Published in Science on August 21, 1987
Structural basis of substrate specificity in the serine proteases. Protein Sci (1995) 4.22
Expression of virus-encoded proteinases: functional and structural similarities with cellular enzymes. Microbiol Rev (1993) 3.33
Site-directed mutagenesis of the proposed catalytic amino acids of the Sindbis virus capsid protein autoprotease. J Virol (1990) 2.11
A conserved histidine is essential for glycerolipid acyltransferase catalysis. J Bacteriol (1998) 1.75
The chemical biology of protein phosphorylation. Annu Rev Biochem (2009) 1.59
Locating active-site hydrogen atoms in D-xylose isomerase: time-of-flight neutron diffraction. Proc Natl Acad Sci U S A (2006) 1.51
Unconventional serine proteases: variations on the catalytic Ser/His/Asp triad configuration. Protein Sci (2008) 1.44
Mutagenesis of essential functional residues in acetylcholinesterase. Proc Natl Acad Sci U S A (1990) 1.23
Poliovirus thiol proteinase 3C can utilize a serine nucleophile within the putative catalytic triad. Proc Natl Acad Sci U S A (1991) 1.20
Cholinesterase-like domains in enzymes and structural proteins: functional and evolutionary relationships and identification of a catalytically essential aspartic acid. Proc Natl Acad Sci U S A (1991) 1.19
Gastrulation defective is a serine protease involved in activating the receptor toll to polarize the Drosophila embryo. Proc Natl Acad Sci U S A (2000) 1.17
Electrostatic complementarity within the substrate-binding pocket of trypsin. Proc Natl Acad Sci U S A (1988) 1.14
Site-directed mutagenesis of dicarboxylic acids near the active site of Bacillus cereus 5/B/6 beta-lactamase II. Biochem J (1991) 1.12
Serine proteases from nematode and protozoan parasites: isolation of sequence homologs using generic molecular probes. Proc Natl Acad Sci U S A (1989) 1.11
Computational design of catalytic dyads and oxyanion holes for ester hydrolysis. J Am Chem Soc (2012) 1.08
The protease and the assembly protein of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8). J Virol (1997) 1.04
Comparison of anionic and cationic trypsinogens: the anionic activation domain is more flexible in solution and differs in its mode of BPTI binding in the crystal structure. Protein Sci (1999) 1.03
The Bacillus subtilis signaling protein SpoIVB defines a new family of serine peptidases. J Bacteriol (2002) 0.96
Allosteric regulation of rhomboid intramembrane proteolysis. EMBO J (2014) 0.96
Roles of Ser101, Asp236, and His237 in catalysis of thioesterase II and of the C-terminal region of the enzyme in its interaction with fatty acid synthase. Proc Natl Acad Sci U S A (1993) 0.95
Characterization of the streptococcal C5a peptidase using a C5a-green fluorescent protein fusion protein substrate. J Bacteriol (2000) 0.91
Histidine pKa shifts accompanying the inactivating Asp121----Asn substitution in a semisynthetic bovine pancreatic ribonuclease. Proc Natl Acad Sci U S A (1991) 0.91
The structure of aspartyl dipeptidase reveals a unique fold with a Ser-His-Glu catalytic triad. Proc Natl Acad Sci U S A (2000) 0.90
Structure and catalysis of acylaminoacyl peptidase: closed and open subunits of a dimer oligopeptidase. J Biol Chem (2010) 0.90
Comparative analysis of mutant tyrosine kinase chemical rescue. Biochemistry (2009) 0.89
A reinvestigation of a synthetic peptide (TrPepz) designed to mimic trypsin. Proc Natl Acad Sci U S A (1994) 0.89
Effect of intermolecular orientation upon proton transfer within a polarizable medium. Biophys J (1991) 0.89
Characterization of a torovirus main proteinase. J Virol (2006) 0.85
A remodelled protease that cleaves phosphotyrosine substrates. J Am Chem Soc (2007) 0.85
Functional dissection of the alphavirus capsid protease: sequence requirements for activity. Virol J (2010) 0.84
Inactivation of the Mycobacterium tuberculosis antigen 85 complex by covalent, allosteric inhibitors. J Biol Chem (2014) 0.83
Unexpected trypsin cleavage at ubiquitinated lysines. Anal Chem (2015) 0.81
Why Ser and not Thr brokers catalysis in the trypsin fold. Biochemistry (2015) 0.80
The pKa of the catalytic histidine residue of chloramphenicol acetyltransferase. Biochem J (1993) 0.80
Characterization of Bafinivirus main protease autoprocessing activities. J Virol (2010) 0.78
From Protease to Decarboxylase: THE MOLECULAR METAMORPHOSIS OF PHOSPHATIDYLSERINE DECARBOXYLASE. J Biol Chem (2015) 0.78
Mutational analysis of the active site of Pseudomonas fluorescens pyrrolidone carboxyl peptidase. J Bacteriol (1996) 0.76
Structure of a switchable subtilisin complexed with a substrate and with the activator azide. Biochemistry (2009) 0.75
Mutational analysis of Cvab, an ABC transporter involved in the secretion of active colicin V. PLoS One (2012) 0.75
Functional role of R462 in the degradation of hyaluronan catalyzed by hyaluronate lyase from Streptococcus pneumoniae. J Mol Model (2015) 0.75
Mass spectrometry-based determination of Kallikrein-related peptidase 7 (KLK7) cleavage preferences and subsite dependency. Sci Rep (2017) 0.75
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry (1979) 180.95
Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes. Cell (1980) 17.55
Rat insulin genes: construction of plasmids containing the coding sequences. Science (1977) 17.05
Polymorphic DNA region adjacent to the 5' end of the human insulin gene. Proc Natl Acad Sci U S A (1981) 12.86
Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose. Cell (1986) 8.87
Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes. Nature (1984) 8.65
Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5' flanking elements. Science (1985) 8.44
The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequences. Nature (1982) 7.47
Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms. Nature (1969) 6.98
Specific inhibition of nuclear RNA polymerase II by alpha-amanitin. Science (1970) 6.39
Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen. Nature (1979) 6.10
Isolation and characterization of a cloned rat insulin gene. Cell (1979) 6.05
Sequence of the human insulin gene. Nature (1980) 5.76
Nucleotide and corresponding amino acid sequences encoded by alpha and beta tubulin mRNAs. Nature (1981) 5.65
Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA. Proc Natl Acad Sci U S A (1978) 5.29
Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs. J Biol Chem (1977) 4.76
Hepatitis B virus gene function: the precore region targets the core antigen to cellular membranes and causes the secretion of the e antigen. Proc Natl Acad Sci U S A (1986) 4.73
Specific nucleolar and nucleoplasmic RNA polymerases. Proc Natl Acad Sci U S A (1970) 4.63
Homology requirements for recombination in Escherichia coli. Proc Natl Acad Sci U S A (1985) 4.46
Structural basis of substrate specificity in the serine proteases. Protein Sci (1995) 4.22
Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos. Science (1992) 4.17
Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions. J Biol Chem (1977) 4.03
A human hepatitis B viral enhancer element. EMBO J (1985) 3.94
Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature (1982) 3.82
Overexpression of HOXB4 in hematopoietic cells causes the selective expansion of more primitive populations in vitro and in vivo. Genes Dev (1995) 3.81
Sequence of protein disulphide isomerase and implications of its relationship to thioredoxin. Nature (1985) 3.75
Redesigning trypsin: alteration of substrate specificity. Science (1985) 3.70
Analysis of the regions flanking the human insulin gene and sequence of an Alu family member. Nucleic Acids Res (1980) 3.55
Insulin-like growth factor II receptor as a multifunctional binding protein. Nature (1987) 3.52
Sequence of the human somatostatin I gene. Science (1984) 3.48
Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning. Biochemistry (1987) 3.48
Tubulin genes are tandemly linked and clustered in the genome of trypanosoma brucei. Cell (1983) 3.40
Transcription of the hepatitis B surface antigen gene in cultured murine cells initiates within the presurface region. J Virol (1984) 3.30
Synthesis of hepatitis B surface antigen in mammalian cells: expression of the entire gene and the coding region. J Virol (1983) 3.24
Pbx proteins display hexapeptide-dependent cooperative DNA binding with a subset of Hox proteins. Genes Dev (1995) 3.13
Synthesis of hepatitis B surface and core antigens in E. coli. Nature (1981) 3.11
A mutational analysis of the insulin gene transcription control region: expression in beta cells is dependent on two related sequences within the enhancer. Proc Natl Acad Sci U S A (1987) 3.08
Differential expression of homeobox genes in functionally distinct CD34+ subpopulations of human bone marrow cells. Proc Natl Acad Sci U S A (1994) 3.08
Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries. Proc Natl Acad Sci U S A (2000) 3.06
An ultrastructural analysis of the developing embryonic pancreas. Dev Biol (1972) 3.00
Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity. Proc Natl Acad Sci U S A (1987) 2.99
Structure of a mouse submaxillary messenger RNA encoding epidermal growth factor and seven related proteins. Science (1983) 2.97
Synergistic activation of the insulin gene by a LIM-homeo domain protein and a basic helix-loop-helix protein: building a functional insulin minienhancer complex. Genes Dev (1992) 2.86
A signal peptide encoded within the precore region of hepatitis B virus directs the secretion of a heterogeneous population of e antigens in Xenopus oocytes. Proc Natl Acad Sci U S A (1988) 2.84
Transcription of hepatitis B virus by RNA polymerase II. Mol Cell Biol (1983) 2.81
Integration of hepatitis B virus sequences and their expression in a human hepatoma cell. Nature (1980) 2.74
Toward molecular mechanisms of developmental processes. Annu Rev Biochem (1973) 2.66
Overexpression of HOXA10 in murine hematopoietic cells perturbs both myeloid and lymphoid differentiation and leads to acute myeloid leukemia. Mol Cell Biol (1997) 2.61
Regulation of rat insulin 1 gene expression: evidence for negative regulation in nonpancreatic cells. Proc Natl Acad Sci U S A (1986) 2.57
AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins. Mol Cell Biol (1997) 2.56
Mice bearing a targeted interruption of the homeobox gene HOXA9 have defects in myeloid, erythroid, and lymphoid hematopoiesis. Blood (1997) 2.55
Splice junctions: association with variation in protein structure. Science (1983) 2.54
Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates. J Biol Chem (2000) 2.50
Synthesis of positional-scanning libraries of fluorogenic peptide substrates to define the extended substrate specificity of plasmin and thrombin. Nat Biotechnol (2000) 2.49
Pbx modulation of Hox homeodomain amino-terminal arms establishes different DNA-binding specificities across the Hox locus. Mol Cell Biol (1996) 2.48
Pan: a transcriptional regulator that binds chymotrypsin, insulin, and AP-4 enhancer motifs. Genes Dev (1990) 2.46
Cloning, sequencing, and chromosomal localization of human term placental alkaline phosphatase cDNA. Proc Natl Acad Sci U S A (1985) 2.44
Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor. Nature (1983) 2.41
Targeting of the hepatitis B virus precore protein to the endoplasmic reticulum membrane: after signal peptide cleavage translocation can be aborted and the product released into the cytoplasm. J Cell Biol (1988) 2.41
Converting trypsin to chymotrypsin: the role of surface loops. Science (1992) 2.40
A sensitive new substrate for chymotrypsin. Anal Biochem (1979) 2.35
Structure and processing of yeast precursor tRNAs containing intervening sequences. Nature (1978) 2.31
Assembly of viral particles in Xenopus oocytes: pre-surface-antigens regulate secretion of the hepatitis B viral surface envelope particle. Proc Natl Acad Sci U S A (1986) 2.28
Structure of two related rat pancreatic trypsin genes. J Biol Chem (1984) 2.24
The subunit structure of mammalian fructose diphosphate aldolase. Biochemistry (1967) 2.24
Frequent co-expression of the HOXA9 and MEIS1 homeobox genes in human myeloid leukemias. Leukemia (1999) 2.21
Structure of a family of rat amylase genes. Nature (1980) 2.19
The minisatellite in the diabetes susceptibility locus IDDM2 regulates insulin transcription. Nat Genet (1995) 2.17
Nucleotide sequence of the yeast 5S ribosomal RNA gene and adjacent putative control regions. Nature (1977) 2.15
Systematic binding analysis of the insulin gene transcription control region: insulin and immunoglobulin enhancers utilize similar transactivators. Mol Cell Biol (1988) 2.13
Cloning and sequence analysis of cDNAs encoding two distinct somatostatin precursors found in the endocrine pancreas of anglerfish. Nature (1980) 2.11
Transactivation by hepatitis B virus X protein is promiscuous and dependent on mitogen-activated cellular serine/threonine kinases. Proc Natl Acad Sci U S A (1993) 2.06
HOXA9 forms triple complexes with PBX2 and MEIS1 in myeloid cells. Mol Cell Biol (1999) 2.06
Evolutionary divergence of substrate specificity within the chymotrypsin-like serine protease fold. J Biol Chem (1997) 2.05
Two related helix-loop-helix proteins participate in separate cell-specific complexes that bind the insulin enhancer. Mol Endocrinol (1991) 2.03
The insulin gene is located on chromosome 11 in humans. Nature (1980) 2.03
Cell-specific enhancers in the rat exocrine pancreas. Proc Natl Acad Sci U S A (1986) 2.02
Reverse biochemistry: use of macromolecular protease inhibitors to dissect complex biological processes and identify a membrane-type serine protease in epithelial cancer and normal tissue. Proc Natl Acad Sci U S A (1999) 2.01
The insulin gene is located on the short arm of chromosome 11 in humans. Diabetes (1981) 2.00
Leukocyte and fibroblast interferon genes are located on human chromosome 9. Proc Natl Acad Sci U S A (1981) 1.98
The insulin and islet amyloid polypeptide genes contain similar cell-specific promoter elements that bind identical beta-cell nuclear complexes. Mol Cell Biol (1992) 1.97
Onset of cell-specific gene expression in the developing mouse pancreas. Proc Natl Acad Sci U S A (1992) 1.96
Hepatitis B viral DNA in liver and serum of asymptomatic carriers. Proc Natl Acad Sci U S A (1982) 1.96
Comparison of the nucleic acid sequence of anglerfish and mammalian insulin mRNA's from cloned cDNA's. Science (1980) 1.92
Changes in the frequency of specific transcripts during development of the pancreas. J Biol Chem (1977) 1.90
Overexpression of the human insulinlike growth factor I receptor promotes ligand-dependent neoplastic transformation. Mol Cell Biol (1990) 1.90
Lineage-specific morphogenesis in the developing pancreas: role of mesenchymal factors. Development (1996) 1.89
Isolation of yeast tRNALeu genes. DNA sequence of a cloned tRNALeu3 gene. J Biol Chem (1979) 1.88
Multiple forms of fructose diphosphate aldolase in mammalian tissues. Proc Natl Acad Sci U S A (1966) 1.88
The three-dimensional structure of Asn102 mutant of trypsin: role of Asp102 in serine protease catalysis. Science (1987) 1.87
The role of HOX homeobox genes in normal and leukemic hematopoiesis. Stem Cells (1996) 1.86
Genes for growth hormone, chorionic somatommammotropin, and growth hormones-like gene on chromosome 17 in humans. Science (1980) 1.85
Homeobox genes in normal hematopoiesis and leukemia. Blood (1992) 1.84