Published in Proc Natl Acad Sci U S A on January 01, 1982
Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A (1986) 32.58
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci U S A (1987) 18.91
High efficiency polyoma DNA transfection of chloroquine treated cells. Nucleic Acids Res (1983) 9.94
New procedure for DNA transfection with polycation and dimethyl sulfoxide. Mol Cell Biol (1984) 8.63
High mutation frequency in DNA transfected into mammalian cells. Proc Natl Acad Sci U S A (1983) 6.05
Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate. Nucleic Acids Res (1983) 5.61
Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation. Nucleic Acids Res (1996) 4.79
Eukaryotic transient expression system dependent on transcription factors and regulatory DNA sequences of vaccinia virus. Proc Natl Acad Sci U S A (1985) 3.61
Transient gene expression control: effects of transfected DNA stability and trans-activation by viral early proteins. Mol Cell Biol (1985) 3.39
How damaged is the biologically active subpopulation of transfected DNA? Mol Cell Biol (1984) 2.88
Expression of a preproinsulin-beta-galactosidase gene fusion in mammalian cells. Proc Natl Acad Sci U S A (1983) 2.57
Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells. Mol Cell Biol (1986) 2.51
Minichromosome assembly of non-integrated plasmid DNA transfected into mammalian cells. Nucleic Acids Res (1985) 2.20
Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency. Mol Cell Biol (1982) 1.94
Adenovirus VA RNAI mediates a translational stimulation which is not restricted to the viral mRNAs. EMBO J (1985) 1.80
Differences in intracellular DNA ligation after microinjection and transfection. Mol Cell Biol (1984) 1.73
Sequence-nonspecific replication of transfected plasmid DNA in poxvirus-infected cells. Proc Natl Acad Sci U S A (1986) 1.71
A polyoma mutant that encodes small T antigen but not middle T antigen demonstrates uncoupling of cell surface and cytoskeletal changes associated with cell transformation. Mol Cell Biol (1984) 1.52
trans-acting factors interact with a cyclic AMP response element to modulate expression of the human gonadotropin alpha gene. Mol Cell Biol (1987) 1.17
Expression and stabilization of microinjected plasmids containing the herpes simplex virus thymidine kinase gene and polyoma virus DNA in mouse cells. Mol Cell Biol (1983) 1.16
Sequences outside of the long terminal repeat determine the lymphomogenic potential of Rous-associated virus type 1. J Virol (1985) 1.08
Fate of exogenous recombinant plasmids introduced into mouse and human cells. Nucleic Acids Res (1985) 1.06
The expression of exogenous genes in macrophages: obstacles and opportunities. Methods Mol Biol (2009) 1.05
Helper activity for gene expression, a novel function of the SV40 enhancer. Nucleic Acids Res (1989) 1.03
Gene recombination in X-ray-sensitive hamster cells. Mol Cell Biol (1987) 1.01
Autophagy induced by calcium phosphate precipitates targets damaged endosomes. J Biol Chem (2014) 0.99
Retroviral transduction of murine primary T lymphocytes. Methods Mol Biol (2009) 0.94
Procaryotic genomic DNA inhibits mammalian cell transformation. Mol Cell Biol (1983) 0.94
Interferon induction by transfection of Sendai virus C gene cDNA. J Virol (1987) 0.91
Gene transfer from targeted liposomes to specific lymphoid cells by electroporation. Proc Natl Acad Sci U S A (1988) 0.91
Pseudotype-based neutralization assays for influenza: a systematic analysis. Front Immunol (2015) 0.88
Polyarginine molecular weight determines transfection efficiency of calcium condensed complexes. Mol Pharm (2013) 0.83
Intracellular trafficking pathways involved in the gene transfer of nano-structured calcium phosphate-DNA particles. Biomaterials (2011) 0.83
Modulation of an ultraviolet mutational hotspot in a shuttle vector Xeroderma cells. Nucleic Acids Res (1991) 0.82
Transfection with extracellularly UV-damaged DNA induces human and rat cells to express a mutator phenotype towards parvovirus H-1. Mol Cell Biol (1984) 0.81
The uptake and stability of simian-virus-40 DNA after calcium phosphate transfection of CV-1 cells. Biochem J (1984) 0.80
Self-assembled lipid nanomedicines for siRNA tumor targeting. J Biomed Nanotechnol (2009) 0.80
Nonviral gene transfer as a tool for studying transcription regulation of xenobiotic metabolizing enzymes. Adv Drug Deliv Rev (2010) 0.79
High efficiency expression of transfected genes in a Drosophila melanogaster haploid (1182) cell line. Nucleic Acids Res (1989) 0.78
Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles. Cytotechnology (2007) 0.77
Inhibition of the herpes simplex virus thymidine kinase gene transfection in Ltk- cells by potential Z-DNA forming polymers. Nucleic Acids Res (1985) 0.77
Transient transfection induces different intracellular calcium signaling in CHO K1 versus HEK 293 cells. Cytotechnology (2000) 0.76
DNA-histone complexes as ligands amplify cell penetration and nuclear targeting of anti-DNA antibodies via energy-independent mechanisms. Immunology (2015) 0.75
Using Pulmozyme DNase treatment in lentiviral vector production. Hum Gene Ther Methods (2012) 0.75
In vitro studies of liposome-mediated gene transfer into head and neck cancer cell lines. Eur Arch Otorhinolaryngol (1997) 0.75
A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology (1973) 127.36
Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell (1978) 19.44
The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. Methods Cell Biol (1975) 6.58
The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell (1978) 6.38
Genetics of human cess line. IV. DNA-mediated heritable transformation of a biochemical trait. Proc Natl Acad Sci U S A (1962) 4.96
Parameters governing the transfer of the genes for thymidine kinase and dihydrofolate reductase into mouse cells using metaphase chromosomes or DNA. Somatic Cell Genet (1980) 4.16
Altering genotype and phenotype by DNA-mediated gene transfer. Science (1980) 3.82
Mechanisms and applications of DNA-mediated gene transfer in mammalian cells - a review. Gene (1981) 3.46
DNA-mediated gene transfer of a circular plasmid into murine cells. Proc Natl Acad Sci U S A (1979) 2.02
Optimal conditions for uptake of exogenous DNA by Chinese hamster lung cells deficient in hypoxanthine-guanine phosphoribosyltransferase. Biochim Biophys Acta (1975) 1.95
Uptake of nonviral nucleic acids by mammalian cells. Prog Nucleic Acid Res Mol Biol (1971) 1.43
Biologic activity of isolated viral nucleic acids. Prog Med Virol (1970) 1.40
Uptake and fate of the DNA of adenovirus type 2 in KB cells. Virology (1975) 1.39
Mobilization of synaptic membrane-bound calcium by acidic amino acids. J Neurochem (1975) 1.09
Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci U S A (1980) 5.47
The status of the gene map of the human chromosomes. Science (1977) 5.44
Somatic cell genetics and gene families. Science (1983) 4.61
A conversational system for the computer analysis of nucleic acid sequences. Nucleic Acids Res (1981) 4.07
Transfer of the human gene for hypoxanthine-guanine phosphoribosyltransferase via isolated human metaphase chromosomes into mouse L-cells. Proc Natl Acad Sci U S A (1975) 3.91
Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene. Proc Natl Acad Sci U S A (1985) 3.91
Mechanisms and applications of DNA-mediated gene transfer in mammalian cells - a review. Gene (1981) 3.46
Gene transfer, expression, and molecular cloning of the human transferrin receptor gene. Cell (1984) 3.42
A review of enzyme polymorphism, linkage and electrophoretic conditions for mouse and somatic cell hybrids in starch gels. J Histochem Cytochem (1973) 3.33
Human nucleolus organizers on nonhomologous chromosomes can share the same ribosomal gene variants. Proc Natl Acad Sci U S A (1981) 3.21
The human transferrin receptor gene: genomic organization, and the complete primary structure of the receptor deduced from a cDNA sequence. Cell (1984) 3.14
Molecular cloning and chromosome mapping of a mouse DNA sequence homologous to homeotic genes of Drosophila. Cell (1984) 2.89
Controlled production of proliferating somatic cell hybrids. J Cell Biol (1970) 2.78
Mammalian Trithorax and polycomb-group homologues are antagonistic regulators of homeotic development. Proc Natl Acad Sci U S A (1999) 2.76
A sequential staining technique for the chromosomal analysis of the interspecific mouse/hamster and mouse/human somatic cell hybrids. Exp Cell Res (1977) 2.71
Chromosomal localization of human beta globin gene on human chromosome 11 in somatic cell hybrids. Proc Natl Acad Sci U S A (1978) 2.65
Regional mapping of the phenylalanine hydroxylase gene and the phenylketonuria locus in the human genome. Proc Natl Acad Sci U S A (1985) 2.58
International system for human gene nomenclature (1979) ISGN (1979). Cytogenet Cell Genet (1979) 2.58
The linkage of genes for the human interferon-induced antiviral protein and indophenol oxidase-B traits to chromosome G-21. J Exp Med (1973) 2.57
Linkage analysis in man by somatic cell genetics. Nature (1973) 2.33
Integration and stable germ line transmission of genes injected into mouse pronuclei. Science (1981) 2.31
Gene transfer into mouse embryos: production of transgenic mice by pronuclear injection. Methods Enzymol (1983) 2.23
Two steps in the evolution of Antennapedia-class vertebrate homeobox genes. Proc Natl Acad Sci U S A (1989) 2.23
Spatial restriction in expression of a mouse homoeo box locus within the central nervous system. Nature (1986) 2.16
Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences. Mol Cell Biol (1985) 2.13
Gene linkage analysis in the mouse by somatic cell hybridization: assignment of adenine phosphoribosyltransferase to chromosome 8 and alpha-galactosidase to the X chromosome. Somatic Cell Genet (1975) 2.11
Chromosomal location of structural genes encoding murine immunoglobulin lambda light chains. Genetics of murine lambda light chains. J Exp Med (1981) 2.07
DNA-mediated gene transfer of a circular plasmid into murine cells. Proc Natl Acad Sci U S A (1979) 2.02
Differential staining of interspecific chromosomes in somatic cell hybrids by alkaline Giemsa stain. Somatic Cell Genet (1976) 1.98
Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells. Proc Natl Acad Sci U S A (1977) 1.97
Assignment of the gene for adenine phosphoribosyltransferase to human chromosome 16 by mouse-human somatic cell hybridization. Proc Natl Acad Sci U S A (1974) 1.97
Glutamate oxalate transaminase (GOT) genetics in Mus musculus: linkage, polymorphism, and phenotypes of the Got-2 and Got-1 loci. Biochem Genet (1970) 1.96
A new era in mammalian gene mapping: somatic cell genetics and recombinant DNA methodologies. Nature (1981) 1.96
Human chromosome 21 dosage: effect on the expression of the interferon induced antiviral state. Science (1974) 1.95
Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids (thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes). Proc Natl Acad Sci U S A (1972) 1.93
Homeo box gene complex on mouse chromosome 11: molecular cloning, expression in embryogenesis, and homology to a human homeo box locus. Cell (1985) 1.92
Chromosomal assignment of the mouse kappa light chain genes. Proc Natl Acad Sci U S A (1979) 1.87
Receptors for human alpha and beta interferon but not for gamma interferon are specified by human chromosome 21. Proc Natl Acad Sci U S A (1984) 1.86
Human chromosome 7 carries the beta 2 interferon gene. Proc Natl Acad Sci U S A (1986) 1.82
Starch gel electrophoretic phenotypes of mouse times human somatic cell hybrids and mouse isozyme polymorphisms. In Vitro (1974) 1.81
Two homoeo box loci mapped in evolutionarily related mouse and human chromosomes. Nature (1985) 1.81
A new vector for recombination-based cloning of large DNA fragments from yeast artificial chromosomes. Nucleic Acids Res (1995) 1.80
A new electrophoretic-autoradiographic method for the visual detection of phosphotransferases. Anal Biochem (1973) 1.78
Chromosomal location of the structural gene cluster encoding murine immunoglobulin heavy chains. J Exp Med (1980) 1.76
Chromosome-mediated gene transfer results in two classes of unstable transformants. Proc Natl Acad Sci U S A (1980) 1.74
Co-transfer of human X-linked markers into murine somatic cells via isolated metaphase chromosomes. Proc Natl Acad Sci U S A (1978) 1.73
Phenotype stabilisation and integration of transferred material in chromosome-mediated gene transfer. Nature (1979) 1.73
Genetic analysis with man-mouse somatic cell hybrids. Linkage between human lactate dehydrogenase A and B and peptidase B. Nature (1970) 1.73
An efficient cis-element discovery method using multiple sequence comparisons based on evolutionary relationships. Genomics (2001) 1.72
Sequence analysis of the murine Hox-2.2, -2.3, and -2.4 homeo boxes: evolutionary and structural comparisons. Genomics (1987) 1.71
Multiplex gene regulation: a two-tiered approach to transgene regulation in transgenic mice. Proc Natl Acad Sci U S A (1989) 1.69
Genetic control of two electrophoretic variants of glucosephosphate isomerase in the mouse (Mus musculus). Biochem Genet (1969) 1.69
Deposits of amyloid beta protein in the central nervous system of transgenic mice. Science (1991) 1.69
Karyotype analysis utilizing differentially stained constitutive heterochromatin of human and murine chromosomes. Chromosoma (1971) 1.68
Cotransfer of thymidine kinase and galactokinase genes by chromosome-mediated gene transfer. Proc Natl Acad Sci U S A (1978) 1.67
Chromosome polymorphism in American Negro and White populations. Nature (1971) 1.66
The evolution of the vertebrate Dlx gene family. Proc Natl Acad Sci U S A (1996) 1.64
Chromosomal abnormalities in the human population: estimation of rates based on New Haven newborn study. Science (1970) 1.64
Biological detection of specific mRNA molecules by microinjection. Proc Natl Acad Sci U S A (1979) 1.63
Cloning and expression of murine lymphotoxin cDNA. J Immunol (1987) 1.62
Parasexual approaches to the genetics of man. Annu Rev Genet (1975) 1.62
Genetic complementation in heterokaryons of human fibroblasts defective in cobalamin metabolism. Proc Natl Acad Sci U S A (1975) 1.62
Expression of liver phenotypes in cultured mouse hepatoma cells. J Natl Cancer Inst (1980) 1.60
Pluripotent teratocarcinoma-thymus somatic cell hybrids. Cell (1976) 1.60
Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique. J Cell Biol (1975) 1.59
Embryonal carcinoma cells (and their somatic cell hybrids) are resistant to infection by the murine parvovirus MVM, which does infect other teratocarcinoma-derived cell lines. J Cell Physiol (1977) 1.58
The use of fluorescence dequenching measurements to follow viral membrane fusion events. Methods Biochem Anal (1988) 1.56
Detection of homeobox genes in development and evolution. Proc Natl Acad Sci U S A (1991) 1.55
A herpes simplex virus 1 integration site in the mouse genome defined by somatic cell genetic analysis. Cell (1978) 1.54
Cyclic changes in enzyme activity in synchronized mammalian cell cultures. Science (1968) 1.53
Chromosomal assignment of a family of human oncogenes. Proc Natl Acad Sci U S A (1983) 1.53
Expansion of the Hox gene family and the evolution of chordates. Proc Natl Acad Sci U S A (1993) 1.52
Assignment of a gene for adenosine deaminase to human chromosome 20. Hum Hered (1974) 1.52
The William Allan Memorial Award address: Reverse genetics and beyond. Am J Hum Genet (1984) 1.52
DNA sequence associated with chromosome translocations in mouse plasmacytomas. Proc Natl Acad Sci U S A (1982) 1.51
Assignment of nucleoside phosphorylase to D-14 and localization of X-linked loci in man by somatic cell genetics. Nat New Biol (1973) 1.51
Tay-Sachs' and Sandhoff's diseases: the assignment of genes for hexosaminidase A and B to individual human chromosomes. Proc Natl Acad Sci U S A (1975) 1.49
Fibroblast interferon in man is coded by two loci on separate chromosomes. Cell (1979) 1.49
Assignment of the gene for galactokinase to human chromosome 17 and its regional localisation to band q21-22. Nature (1974) 1.49
Deoxyribonucleic acid-mediated gene transfer in mammalian cells: molecular analysis of unstable transformants and their progression to stability. Mol Cell Biol (1981) 1.47
The somatic cell genetics of human interferon: assignment of human interferon loci to chromosomes 2 and 5. Proc Natl Acad Sci U S A (1974) 1.46
Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids. Proc Natl Acad Sci U S A (1977) 1.45
The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2, and skeletal muscle actin are located on three different mouse chromosomes. EMBO J (1982) 1.44
Isolation, chromosomal localization, and nucleotide sequence of the human HOX 1.4 homeobox. Genomics (1989) 1.43
Early regions of JC virus and BK virus induce distinct and tissue-specific tumors in transgenic mice. Proc Natl Acad Sci U S A (1986) 1.43
"Ultramicroinjection" of macromolecules or small particles into animal cells. A new technique based on virus-induced cell fusion. J Cell Biol (1975) 1.43
The PKU locus in man is on chromosome 12. Am J Hum Genet (1984) 1.42
Localization and induction of the human thymidine kinase gene by adenovirus 12. Nat New Biol (1973) 1.42
Transcriptional regulation by iron of the gene for the transferrin receptor. Mol Cell Biol (1986) 1.42
Phylogenetic reconstruction of vertebrate Hox cluster duplications. Mol Biol Evol (1997) 1.41
Murine alpha-fetoprotein and albumin: two evolutionarily linked proteins encoded on the same mouse chromosome. Somatic Cell Genet (1981) 1.41
Identification of human genomic clones coding the major histocompatibility antigens HLA-a2 and HLA-B7 by DNA-mediated gene transfer. Proc Natl Acad Sci U S A (1982) 1.39
Linkage of isozyyme loci in the mouse: phosphoglucomutase-2 (Pgm-2), mitochondrial NADP malate dehydrogenase (Mod-2), and dipeptidase-1 (Dip-1). Biochem Genet (1971) 1.37
Linkage analysis using somatic cell hybrids. Adv Hum Genet (1972) 1.36
Modification of expression and cis-regulation of Hoxc8 in the evolution of diverged axial morphology. Proc Natl Acad Sci U S A (1998) 1.36
The early region of human papovavirus JC induces dysmyelination in transgenic mice. Cell (1986) 1.35
Antibodies to a cell-surface component coded by human chromosome 21 inhibit action of interferon. Nature (1976) 1.35