Human immunodeficiency virus type 1 integrase: effects of mutations on viral ability to integrate, direct viral gene expression from unintegrated viral DNA templates, and sustain viral propagation in primary cells.

PubWeight™: 3.56‹?› | Rank: Top 1%

🔗 View Article (PMC 188585)

Published in J Virol on January 01, 1995

Authors

M Wiskerchen1, M A Muesing

Author Affiliations

1: Lilly Research Laboratories, Indianapolis, Indiana 46285-0438.

Articles citing this

(truncated to the top 100)

Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication. J Virol (1995) 4.27

Role of the non-homologous DNA end joining pathway in the early steps of retroviral infection. EMBO J (2001) 3.60

Diketo acid inhibitor mechanism and HIV-1 integrase: implications for metal binding in the active site of phosphotransferase enzymes. Proc Natl Acad Sci U S A (2002) 3.40

Efficient HIV-1 replication can occur in the absence of the viral matrix protein. EMBO J (1998) 3.30

Analysis of early human immunodeficiency virus type 1 DNA synthesis by use of a new sensitive assay for quantifying integrated provirus. J Virol (2003) 3.18

Transduction of nondividing cells using pseudotyped defective high-titer HIV type 1 particles. Proc Natl Acad Sci U S A (1996) 3.07

HIV-1 integrase inhibitors that compete with the target DNA substrate define a unique strand transfer conformation for integrase. Proc Natl Acad Sci U S A (2000) 3.00

Human immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate viral DNA efficiently during infection. J Virol (1996) 2.82

SAMHD1-deficient CD14+ cells from individuals with Aicardi-Goutières syndrome are highly susceptible to HIV-1 infection. PLoS Pathog (2011) 2.65

Integration is required for productive infection of monocyte-derived macrophages by human immunodeficiency virus type 1. J Virol (1995) 2.43

Critical contacts between HIV-1 integrase and viral DNA identified by structure-based analysis and photo-crosslinking. EMBO J (1997) 2.42

Genetic analysis of human immunodeficiency virus type 1 integrase and the U3 att site: unusual phenotype of mutants in the zinc finger-like domain. J Virol (1995) 2.22

Human immunodeficiency virus type 1 integrase protein promotes reverse transcription through specific interactions with the nucleoprotein reverse transcription complex. J Virol (1999) 2.16

The cell cycle independence of HIV infections is not determined by known karyophilic viral elements. PLoS Pathog (2005) 2.02

The G140S mutation in HIV integrases from raltegravir-resistant patients rescues catalytic defect due to the resistance Q148H mutation. Nucleic Acids Res (2009) 2.00

The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication. PLoS Pathog (2008) 2.00

Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication. J Virol (2004) 1.94

Detecting HIV-1 integration by repetitive-sampling Alu-gag PCR. Methods (2009) 1.91

Effect of plasmid DNA vaccine design and in vivo electroporation on the resulting vaccine-specific immune responses in rhesus macaques. J Virol (2007) 1.87

Requirement for integrase during reverse transcription of human immunodeficiency virus type 1 and the effect of cysteine mutations of integrase on its interactions with reverse transcriptase. J Virol (2004) 1.79

Lentiviral vectors with a defective integrase allow efficient and sustained transgene expression in vitro and in vivo. Proc Natl Acad Sci U S A (2006) 1.74

Lack of integrase can markedly affect human immunodeficiency virus type 1 particle production in the presence of an active viral protease. J Virol (1996) 1.71

Nuclear localization of human immunodeficiency virus type 1 preintegration complexes (PICs): V165A and R166A are pleiotropic integrase mutants primarily defective for integration, not PIC nuclear import. J Virol (2002) 1.68

Human immunodeficiency virus type 1 replication in the absence of integrase-mediated dna recombination: definition of permissive and nonpermissive T-cell lines. J Virol (2001) 1.63

Early transcription from nonintegrated DNA in human immunodeficiency virus infection. J Virol (2003) 1.57

Unintegrated lentivirus DNA persistence and accessibility to expression in nondividing cells: analysis with class I integrase mutants. J Virol (2004) 1.56

Directed integration of viral DNA mediated by fusion proteins consisting of human immunodeficiency virus type 1 integrase and Escherichia coli LexA protein. J Virol (1996) 1.55

Identification of critical amino acid residues in human immunodeficiency virus type 1 IN required for efficient proviral DNA formation at steps prior to integration in dividing and nondividing cells. J Virol (2000) 1.53

Accumulation and intranuclear distribution of unintegrated human immunodeficiency virus type 1 DNA. J Virol (2001) 1.53

Viral complementation allows HIV-1 replication without integration. Retrovirology (2008) 1.52

Evidence for gene expression by unintegrated human immunodeficiency virus type 1 DNA species. J Virol (2004) 1.49

Integrase and integration: biochemical activities of HIV-1 integrase. Retrovirology (2008) 1.48

Integration-deficient lentiviral vectors: a slow coming of age. Mol Ther (2009) 1.47

Human macrophages support persistent transcription from unintegrated HIV-1 DNA. Virology (2007) 1.46

Conserved sequences in the carboxyl terminus of integrase that are essential for human immunodeficiency virus type 1 replication. J Virol (1996) 1.44

Enhancement of human immunodeficiency virus type 1 infection by the CC-chemokine RANTES is independent of the mechanism of virus-cell fusion. J Virol (1999) 1.42

Effects of mutations in residues near the active site of human immunodeficiency virus type 1 integrase on specific enzyme-substrate interactions. J Virol (1998) 1.40

Efficient magnesium-dependent human immunodeficiency virus type 1 integrase activity. J Virol (1995) 1.38

Transduction of nondividing human macrophages with gammaretrovirus-derived vectors. J Virol (2006) 1.38

HIV-1 gene expression: lessons from provirus and non-integrated DNA. Retrovirology (2004) 1.36

Human immunodeficiency virus type 1 (HIV-1) Vpr enhances expression from unintegrated HIV-1 DNA. J Virol (2003) 1.31

Genetic analyses of DNA-binding mutants in the catalytic core domain of human immunodeficiency virus type 1 integrase. J Virol (2005) 1.30

Human immunodeficiency virus type 1 incorporated with fusion proteins consisting of integrase and the designed polydactyl zinc finger protein E2C can bias integration of viral DNA into a predetermined chromosomal region in human cells. J Virol (2006) 1.30

Comparative analysis of immune responses induced by vaccination with SIV antigens by recombinant Ad5 vector or plasmid DNA in rhesus macaques. Mol Ther (2010) 1.28

Genetic analyses of conserved residues in the carboxyl-terminal domain of human immunodeficiency virus type 1 integrase. J Virol (2005) 1.28

Natural polymorphisms of human immunodeficiency virus type 1 integrase and inherent susceptibilities to a panel of integrase inhibitors. Antimicrob Agents Chemother (2009) 1.27

Structure-based mutagenesis of the catalytic domain of human immunodeficiency virus type 1 integrase. J Virol (1997) 1.27

A large U3 deletion causes increased in vivo expression from a nonintegrating lentiviral vector. Mol Ther (2008) 1.23

The role of unintegrated DNA in HIV infection. Retrovirology (2011) 1.23

Progress toward a human CD4/CCR5 transgenic rat model for de novo infection by human immunodeficiency virus type 1. J Exp Med (2002) 1.23

Evaluation of the functional involvement of human immunodeficiency virus type 1 integrase in nuclear import of viral cDNA during acute infection. J Virol (2004) 1.18

Immunization delivered by lentiviral vectors for cancer and infectious diseases. Immunol Rev (2011) 1.18

LEDGINs inhibit late stage HIV-1 replication by modulating integrase multimerization in the virions. Retrovirology (2013) 1.17

Mapping HIV-1 vaccine induced T-cell responses: bias towards less-conserved regions and potential impact on vaccine efficacy in the Step study. PLoS One (2011) 1.16

Direct evidence of lower viral replication rates in vivo in human immunodeficiency virus type 2 (HIV-2) infection than in HIV-1 infection. J Virol (2007) 1.16

Concerted action of cellular JNK and Pin1 restricts HIV-1 genome integration to activated CD4+ T lymphocytes. Nat Med (2010) 1.14

LFA-1 is a key determinant for preferential infection of memory CD4+ T cells by human immunodeficiency virus type 1. J Virol (2005) 1.13

Circular forms of unintegrated human immunodeficiency virus type 1 DNA and high levels of viral protein expression: association with dementia and multinucleated giant cells in the brains of patients with AIDS. J Virol (1997) 1.11

Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection. J Virol (1996) 1.10

Identification and characterization of a double-stranded RNA- reovirus temperature-sensitive mutant defective in minor core protein mu2. J Virol (1996) 1.09

The role of manganese in promoting multimerization and assembly of human immunodeficiency virus type 1 integrase as a catalytically active complex on immobilized long terminal repeat substrates. J Virol (1996) 1.09

Rescue of multiple viral functions by a second-site suppressor of a human immunodeficiency virus type 1 nucleocapsid mutation. J Virol (2000) 1.06

Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import. Retrovirology (2005) 1.04

Identification and characterization of a temperature-sensitive mutant of human immunodeficiency virus type 1 by alanine scanning mutagenesis of the integrase gene. J Virol (1995) 1.04

Host and viral determinants for MxB restriction of HIV-1 infection. Retrovirology (2014) 1.03

Moloney murine leukemia virus integrase protein augments viral DNA synthesis in infected cells. J Virol (2001) 1.03

Molecular mechanisms by which human immunodeficiency virus type 1 integrase stimulates the early steps of reverse transcription. J Virol (2007) 1.03

A three-dimensional model of the human immunodeficiency virus type 1 integration complex. J Comput Aided Mol Des (2005) 1.02

Effect of polypurine tract (PPT) mutations on human immunodeficiency virus type 1 replication: a virus with a completely randomized PPT retains low infectivity. J Virol (2005) 1.01

Incorporation of functional human immunodeficiency virus type 1 integrase into virions independent of the Gag-Pol precursor protein. J Virol (1997) 1.01

Relief of preintegration inhibition and characterization of additional blocks for HIV replication in primary mouse T cells. PLoS One (2008) 1.01

Measurement of human immunodeficiency virus type 1 preintegration transcription by using Rev-dependent Rev-CEM cells reveals a sizable transcribing DNA population comparable to that from proviral templates. J Virol (2009) 1.01

Mutations in nonconserved domains of Ty3 integrase affect multiple stages of the Ty3 life cycle. J Virol (1999) 1.00

The role of lysine 186 in HIV-1 integrase multimerization. Virology (2007) 1.00

Allosteric inhibitor development targeting HIV-1 integrase. ChemMedChem (2011) 1.00

Effect of DNA repair protein Rad18 on viral infection. PLoS Pathog (2006) 0.99

A dynamic model of HIV integrase inhibition and drug resistance. J Mol Biol (2010) 0.99

Biochemical analysis of HIV-1 integrase variants resistant to strand transfer inhibitors. J Biol Chem (2008) 0.98

Sequential deletion of the integrase (Gag-Pol) carboxyl terminus reveals distinct phenotypic classes of defective HIV-1. J Virol (2011) 0.97

Vpr is required for efficient Nef expression from unintegrated human immunodeficiency virus type 1 DNA. J Virol (2007) 0.97

How HIV takes advantage of the cytoskeleton in entry and replication. Viruses (2011) 0.96

Ty3 integrase mutants defective in reverse transcription or 3'-end processing of extrachromosomal Ty3 DNA. J Virol (1996) 0.96

An HIV-1 replication pathway utilizing reverse transcription products that fail to integrate. J Virol (2013) 0.94

Simian virus 40-based replication of catalytically inactive human immunodeficiency virus type 1 integrase mutants in nonpermissive T cells and monocyte-derived macrophages. J Virol (2004) 0.94

Design of a novel integration-deficient lentivector technology that incorporates genetic and posttranslational elements to target human dendritic cells. Mol Ther (2013) 0.93

Persistent transcription of a nonintegrating mutant of simian immunodeficiency virus in rhesus macrophages. Virology (2007) 0.93

Posttranslational acetylation of the human immunodeficiency virus type 1 integrase carboxyl-terminal domain is dispensable for viral replication. J Virol (2006) 0.93

Correlation of recombinant integrase activity and functional preintegration complex formation during acute infection by replication-defective integrase mutant human immunodeficiency virus. J Virol (2012) 0.92

Multimodal mechanism of action of allosteric HIV-1 integrase inhibitors. Expert Rev Mol Med (2013) 0.92

Reversion of a human immunodeficiency virus type 1 integrase mutant at a second site restores enzyme function and virus infectivity. J Virol (1996) 0.92

A novel function for spumaretrovirus integrase: an early requirement for integrase-mediated cleavage of 2 LTR circles. Retrovirology (2005) 0.92

Expression of Nef from unintegrated HIV-1 DNA downregulates cell surface CXCR4 and CCR5 on T-lymphocytes. Retrovirology (2010) 0.91

Integrase-lexA fusion proteins incorporated into human immunodeficiency virus type 1 that contains a catalytically inactive integrase gene are functional to mediate integration. J Virol (2000) 0.91

The second chance story of HIV-1 DNA: Unintegrated? Not a problem! Retrovirology (2008) 0.91

Inhibition of human immunodeficiency virus type 1 integrase by the Fab fragment of a specific monoclonal antibody suggests that different multimerization states are required for different enzymatic functions. J Virol (1996) 0.89

Virological and preclinical characterization of a dendritic cell targeting, integration-deficient lentiviral vector for cancer immunotherapy. J Immunother (2015) 0.89

Identification of a nucleotide binding site in HIV-1 integrase. Proc Natl Acad Sci U S A (1998) 0.89

Pharmacovirological impact of an integrase inhibitor on human immunodeficiency virus type 1 cDNA species in vivo. J Virol (2009) 0.88

Inhibition of early stages of HIV-1 assembly by INI1/hSNF5 transdominant negative mutant S6. J Virol (2010) 0.87

Inhibition of early and late events of the HIV-1 replication cycle by cytoplasmic Fab intrabodies against the matrix protein, p17. Mol Med (1997) 0.86

Articles cited by this

A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res (1979) 214.95

Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene. J Virol (1992) 12.89

Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus. Nature (1985) 12.08

High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science (1989) 8.73

Retroviral DNA integration: structure of an integration intermediate. Cell (1988) 8.48

Retroviral integration: structure of the initial covalent product and its precursor, and a role for the viral IN protein. Proc Natl Acad Sci U S A (1989) 8.13

The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro. Cell (1990) 7.99

The avian retroviral IN protein is both necessary and sufficient for integrative recombination in vitro. Cell (1990) 7.19

Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes. Proc Natl Acad Sci U S A (1986) 6.94

Human immunodeficiency virus integration protein expressed in Escherichia coli possesses selective DNA cleaving activity. Proc Natl Acad Sci U S A (1990) 6.85

The avian retroviral integration protein cleaves the terminal sequences of linear viral DNA at the in vivo sites of integration. J Virol (1989) 6.83

Residues critical for retroviral integrative recombination in a region that is highly conserved among retroviral/retrotransposon integrases and bacterial insertion sequence transposases. Mol Cell Biol (1992) 6.77

Activities of human immunodeficiency virus (HIV) integration protein in vitro: specific cleavage and integration of HIV DNA. Proc Natl Acad Sci U S A (1991) 6.58

HIV-1 DNA integration: mechanism of viral DNA cleavage and DNA strand transfer. Cell (1991) 6.54

Association of integrase, matrix, and reverse transcriptase antigens of human immunodeficiency virus type 1 with viral nucleic acids following acute infection. Proc Natl Acad Sci U S A (1993) 6.44

Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro. J Virol (1992) 6.07

Structure of the termini of DNA intermediates in the integration of retroviral DNA: dependence on IN function and terminal DNA sequence. Cell (1989) 6.04

Retroviral DNA integration directed by HIV integration protein in vitro. Science (1990) 5.87

Molecular characterization of human immunodeficiency virus type 1 cloned directly from uncultured human brain tissue: identification of replication-competent and -defective viral genomes. J Virol (1991) 5.56

The human immunodeficiency virus-1 nef gene product: a positive factor for viral infection and replication in primary lymphocytes and macrophages. J Exp Med (1994) 5.22

Construction and use of a human immunodeficiency virus vector for analysis of virus infectivity. J Virol (1990) 5.01

Reversal of integration and DNA splicing mediated by integrase of human immunodeficiency virus. Science (1992) 4.97

Regulatory pathways governing HIV-1 replication. Cell (1989) 4.97

The terminal nucleotides of retrovirus DNA are required for integration but not virus production. Nature (1984) 4.89

Pseudotyping with human T-cell leukemia virus type I broadens the human immunodeficiency virus host range. J Virol (1991) 4.74

The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation. Proc Natl Acad Sci U S A (1991) 4.66

Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex. EMBO J (1993) 4.39

Retroviral integrase domains: DNA binding and the recognition of LTR sequences. Nucleic Acids Res (1991) 4.39

Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding. Proc Natl Acad Sci U S A (1993) 3.99

Requirement of active human immunodeficiency virus type 1 integrase enzyme for productive infection of human T-lymphoid cells. J Virol (1992) 3.85

Substrate specificity of recombinant human immunodeficiency virus integrase protein. J Virol (1991) 3.63

Mutational analysis of the integrase protein of human immunodeficiency virus type 2. Proc Natl Acad Sci U S A (1992) 3.55

Identification of amino acid residues critical for endonuclease and integration activities of HIV-1 IN protein in vitro. Virology (1992) 3.43

Identification of the catalytic and DNA-binding region of the human immunodeficiency virus type I integrase protein. Nucleic Acids Res (1993) 3.42

Integration is not necessary for expression of human immunodeficiency virus type 1 protein products. J Virol (1990) 3.32

Complementation between HIV integrase proteins mutated in different domains. EMBO J (1993) 3.32

Integration of mini-retroviral DNA: a cell-free reaction for biochemical analysis of retroviral integration. Proc Natl Acad Sci U S A (1989) 3.28

Characterization of human immunodeficiency virus type 1 integrase expressed in Escherichia coli and analysis of variants with amino-terminal mutations. J Virol (1993) 3.28

Retroviral reverse transcription and integration: progress and problems. Annu Rev Cell Biol (1992) 3.26

Genetics of retroviral integration. Annu Rev Genet (1992) 3.16

Site-specific hydrolysis and alcoholysis of human immunodeficiency virus DNA termini mediated by the viral integrase protein. Nucleic Acids Res (1991) 2.93

Complete nucleotide sequence, genome organization, and biological properties of human immunodeficiency virus type 1 in vivo: evidence for limited defectiveness and complementation. J Virol (1992) 2.86

Site-directed mutagenesis of HIV-1 integrase demonstrates differential effects on integrase functions in vitro. J Biol Chem (1993) 2.69

Both substrate and target oligonucleotide sequences affect in vitro integration mediated by human immunodeficiency virus type 1 integrase protein produced in Saccharomyces cerevisiae. J Virol (1992) 2.64

Structural implications of spectroscopic characterization of a putative zinc finger peptide from HIV-1 integrase. J Biol Chem (1992) 2.52

A systematic mutational analysis of hormone-binding determinants in the human growth hormone receptor. Proc Natl Acad Sci U S A (1991) 2.43

Genetic analysis of the human immunodeficiency virus type 1 integrase protein. J Virol (1994) 2.26

Removal of 3'-OH-terminal nucleotides from blunt-ended long terminal repeat termini by the avian retrovirus integration protein. J Virol (1990) 2.25

Integration is essential for efficient gene expression of human immunodeficiency virus type 1. J Virol (1993) 2.22

A single-stranded gap in human immunodeficiency virus unintegrated linear DNA defined by a central copy of the polypurine tract. J Virol (1991) 1.90

Human immunodeficiency virus type 1 integrase: effect on viral replication of mutations at highly conserved residues. J Virol (1994) 1.84

Genetic analysis of homomeric interactions of human immunodeficiency virus type 1 integrase using the yeast two-hybrid system. Proc Natl Acad Sci U S A (1993) 1.65

Identification and characterization of a temperature-sensitive mutant of human immunodeficiency virus type 1 by alanine scanning mutagenesis of the integrase gene. J Virol (1995) 1.04

Articles by these authors

Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus. Nature (1985) 12.08

Regulation of mRNA accumulation by a human immunodeficiency virus trans-activator protein. Cell (1987) 11.15

The specificity of the human immunodeficiency virus type 2 transactivator is different from that of human immunodeficiency virus type 1. EMBO J (1987) 2.85

Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter. Proc Natl Acad Sci U S A (1982) 2.56

Viracept (nelfinavir mesylate, AG1343): a potent, orally bioavailable inhibitor of HIV-1 protease. J Med Chem (1997) 2.04

Temperature-sensitive copy number mutants of CoIE1 are located in an untranslated region of the plasmid genome. Proc Natl Acad Sci U S A (1982) 1.83

Complementation of integrase function in HIV-1 virions. EMBO J (1997) 1.46

Enhancement of human immunodeficiency virus type 1 infection by the CC-chemokine RANTES is independent of the mechanism of virus-cell fusion. J Virol (1999) 1.42

Degradation of HIV-1 integrase by the N-end rule pathway. J Biol Chem (2000) 1.31

Identification and characterization of a temperature-sensitive mutant of human immunodeficiency virus type 1 by alanine scanning mutagenesis of the integrase gene. J Virol (1995) 1.04

Inhibition of influenza virus hemagglutinin-mediated membrane fusion by a compound related to podocarpic acid. Virology (1998) 1.02

Phenethylthiazolylthiourea (PETT) compounds as a new class of HIV-1 reverse transcriptase inhibitors. 2. Synthesis and further structure-activity relationship studies of PETT analogs. J Med Chem (1996) 0.79

Potent human immunodeficiency virus type 1 protease inhibitors that utilize noncoded D-amino acids as P2/P3 ligands. J Med Chem (1996) 0.79