Evolutionarily conserved Galphabetagamma binding surfaces support a model of the G protein-receptor complex.

PubWeight™: 1.18‹?› | Rank: Top 10%

🔗 View Article (PMC 38775)

Published in Proc Natl Acad Sci U S A on July 23, 1996

Authors

O Lichtarge1, H R Bourne, F E Cohen

Author Affiliations

1: Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA.

Articles citing this

Characterization of retinal guanylate cyclase-activating protein 3 (GCAP3) from zebrafish to man. Eur J Neurosci (2002) 1.52

Structural and functional constraints in the evolution of protein families. Nat Rev Mol Cell Biol (2009) 1.35

Evolutionary constraints associated with functional specificity of the CMGC protein kinases MAPK, CDK, GSK, SRPK, DYRK, and CK2alpha. Protein Sci (2004) 1.33

HotPatch: a statistical approach to finding biologically relevant features on protein surfaces. J Mol Biol (2007) 1.32

Diversity of guanylate cyclase-activating proteins (GCAPs) in teleost fish: characterization of three novel GCAPs (GCAP4, GCAP5, GCAP7) from zebrafish (Danio rerio) and prediction of eight GCAPs (GCAP1-8) in pufferfish (Fugu rubripes). J Mol Evol (2004) 1.32

ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules. Nucleic Acids Res (2016) 1.18

SiteLight: binding-site prediction using phage display libraries. Protein Sci (2003) 1.17

A regulator of G protein signaling interaction surface linked to effector specificity. Proc Natl Acad Sci U S A (2000) 1.15

Computational methods in drug design: modeling G protein-coupled receptor monomers, dimers, and oligomers. AAPS J (2006) 1.09

Recurrent use of evolutionary importance for functional annotation of proteins based on local structural similarity. Protein Sci (2006) 1.08

JEvTrace: refinement and variations of the evolutionary trace in JAVA. Genome Biol (2002) 0.99

Evolution: a guide to perturb protein function and networks. Curr Opin Struct Biol (2010) 0.97

Helix dipole movement and conformational variability contribute to allosteric GDP release in Galphai subunits. Biochemistry (2009) 0.97

Sequence and structure continuity of evolutionary importance improves protein functional site discovery and annotation. Protein Sci (2010) 0.96

Accurate protein structure annotation through competitive diffusion of enzymatic functions over a network of local evolutionary similarities. PLoS One (2010) 0.96

Structural determinants involved in the formation and activation of G protein betagamma dimers. Neurosignals (2009) 0.95

Evolutionary trace for prediction and redesign of protein functional sites. Methods Mol Biol (2012) 0.94

Evolution of a signaling nexus constrained by protein interfaces and conformational States. PLoS Comput Biol (2010) 0.93

Efficient signal transduction by a chimeric yeast-mammalian G protein alpha subunit Gpa1-Gsalpha covalently fused to the yeast receptor Ste2. EMBO J (1997) 0.90

Solution structure of inhibitor-free human metalloelastase (MMP-12) indicates an internal conformational adjustment. J Mol Biol (2007) 0.89

Conditional activation defect of a human Gsalpha mutant. Proc Natl Acad Sci U S A (1997) 0.88

Rod and cone opsin families differ in spectral tuning domains but not signal transducing domains as judged by saturated evolutionary trace analysis. J Mol Evol (2005) 0.88

The use of evolutionary patterns in protein annotation. Curr Opin Struct Biol (2012) 0.87

Prediction and experimental validation of enzyme substrate specificity in protein structures. Proc Natl Acad Sci U S A (2013) 0.87

Combination of multiple alignment analysis and surface mapping paves a way for a detailed pathway reconstruction--the case of VHL (von Hippel-Lindau) protein and angiogenesis regulatory pathway. Protein Sci (2004) 0.86

Analysis of the receptor binding domain of Gpa1p, the G(alpha) subunit involved in the yeast pheromone response pathway. Mol Cell Biol (1997) 0.85

A novel method for protein-protein interaction site prediction using phylogenetic substitution models. Proteins (2011) 0.83

Prediction of protein-protein interfaces on G-protein beta subunits reveals a novel phospholipase C beta2 binding domain. J Mol Biol (2009) 0.82

Prediction and redesign of protein-protein interactions. Prog Biophys Mol Biol (2014) 0.80

Evolutionary trace analysis of ionotropic glutamate receptor sequences and modeling the interactions of agonists with different NMDA receptor subunits. J Mol Model (2004) 0.80

Distinct interactions between the human adrenergic beta(2) receptor and Galpha(s)--an in silico study. J Mol Model (2010) 0.80

Electrostatic and functional analysis of the seven-bladed WD beta-propellers. Evol Bioinform Online (2008) 0.79

Allosteric mechanisms of G protein-Coupled Receptor signaling: a structural perspective. Methods Mol Biol (2012) 0.78

A transient interaction between the phosphate binding loop and switch I contributes to the allosteric network between receptor and nucleotide in Gαi1. J Biol Chem (2014) 0.77

Gα16 interacts with tetratricopeptide repeat 1 (TPR1) through its β3 region to activate Ras independently of phospholipase Cβ signaling. BMC Struct Biol (2011) 0.77

Molecular modeling studies give hint for the existence of a symmetric hβ₂R-Gαβγ-homodimer. J Mol Model (2013) 0.77

Effects of mutations in the N terminal region of the yeast G protein alpha-subunit Gpa1p on signaling by pheromone receptors. Mol Genet Genomics (2004) 0.76

Structural features of parathyroid hormone receptor coupled to Galpha(s)-protein. Biophys J (2006) 0.76

Methods used to study the oligomeric structure of G protein-coupled receptors. Biosci Rep (2017) 0.75

Articles cited by this

Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A (1988) 193.60

A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res (1984) 181.98

Progressive sequence alignment as a prerequisite to correct phylogenetic trees. J Mol Evol (1987) 41.41

Fast and sensitive multiple sequence alignments on a microcomputer. Comput Appl Biosci (1989) 24.33

The GTPase superfamily: conserved structure and molecular mechanism. Nature (1991) 19.64

The relation between the divergence of sequence and structure in proteins. EMBO J (1986) 16.66

Areas, volumes, packing and protein structure. Annu Rev Biophys Bioeng (1977) 16.38

An evolutionary trace method defines binding surfaces common to protein families. J Mol Biol (1996) 9.31

The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2. Cell (1995) 7.66

The 2.0 A crystal structure of a heterotrimeric G protein. Nature (1996) 6.77

The 2.2 A crystal structure of transducin-alpha complexed with GTP gamma S. Nature (1993) 4.83

Heterotrimeric G proteins: organizers of transmembrane signals. Cell (1995) 4.55

Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysis. Science (1994) 4.43

Structure-based strategies for drug design and discovery. Science (1992) 3.72

GTPase mechanism of Gproteins from the 1.7-A crystal structure of transducin alpha-GDP-AIF-4. Nature (1994) 3.66

Substitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Nature (1993) 3.57

Loops in globular proteins: a novel category of secondary structure. Science (1986) 3.48

Projection structure of rhodopsin. Nature (1993) 3.41

Structural determinants for activation of the alpha-subunit of a heterotrimeric G protein. Nature (1994) 3.13

Myristoylated alpha subunits of guanine nucleotide-binding regulatory proteins. Proc Natl Acad Sci U S A (1987) 2.93

Alternative splicing of C-terminal tail of prostaglandin E receptor subtype EP3 determines G-protein specificity. Nature (1993) 2.91

Site of G protein binding to rhodopsin mapped with synthetic peptides from the alpha subunit. Science (1988) 2.66

Lipid modifications of trimeric G proteins. J Biol Chem (1995) 2.39

Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors. Cell (1993) 2.34

Rhodopsin, photoreceptor of the rod cell. An emerging pattern for structure and function. J Biol Chem (1992) 2.24

G-protein alpha-subunit expression, myristoylation, and membrane association in COS cells. Proc Natl Acad Sci U S A (1990) 2.13

G proteins in signal transduction. Annu Rev Pharmacol Toxicol (1990) 1.85

Projection structure of frog rhodopsin in two crystal forms. Proc Natl Acad Sci U S A (1995) 1.45

NMR structure of a receptor-bound G-protein peptide. Nature (1993) 1.34

Chimeric muscarinic cholinergic: beta-adrenergic receptors that activate Gs in response to muscarinic agonists. J Biol Chem (1990) 1.32

Transducin-alpha C-terminal mutations prevent activation by rhodopsin: a new assay using recombinant proteins expressed in cultured cells. EMBO J (1995) 1.18

Mutagenesis of the beta 2-adrenergic receptor: how structure elucidates function. Annu Rev Pharmacol Toxicol (1992) 1.11

Tryptophan W207 in transducin T alpha is the fluorescence sensor of the G protein activation switch and is involved in the effector binding. EMBO J (1993) 1.11

Synthetic peptides as probes for G protein function. Carboxyl-terminal G alpha s peptides mimic Gs and evoke high affinity agonist binding to beta-adrenergic receptors. J Biol Chem (1994) 1.07

Identification of effector-activating residues of Gs alpha. Cell (1992) 1.05

Genetic identification of residues involved in association of alpha and beta G-protein subunits. Mol Cell Biol (1994) 1.02

A site on rod G protein alpha subunit that mediates effector activation. Science (1992) 1.01

The evolution and structure of aminergic G protein-coupled receptors. Receptors Channels (1994) 1.01

Rhodopsin/transducin interactions. II. Influence of the transducin-beta gamma subunit complex on the coupling of the transducin-alpha subunit to rhodopsin. J Biol Chem (1992) 1.00

Rhodopsin/transducin interactions. I. Characterization of the binding of the transducin-beta gamma subunit complex to rhodopsin using fluorescence spectroscopy. J Biol Chem (1992) 1.00

G-protein alpha o subunit: mutation of conserved cysteines identifies a subunit contact surface and alters GDP affinity. Proc Natl Acad Sci U S A (1993) 0.99

Binding of an alpha 2 adrenergic receptor third intracellular loop peptide to G beta and the amino terminus of G alpha. J Biol Chem (1994) 0.98

A site on transducin alpha-subunit of interaction with the polycationic region of cGMP phosphodiesterase inhibitory subunit. J Biol Chem (1993) 0.97

GTPases: a family of molecular switches and clocks. Philos Trans R Soc Lond B Biol Sci (1995) 0.94

The entropic tension of protein loops. J Mol Biol (1990) 0.77

Articles by these authors

Conversion of alpha-helices into beta-sheets features in the formation of the scrapie prion proteins. Proc Natl Acad Sci U S A (1993) 10.36

An evolutionary trace method defines binding surfaces common to protein families. J Mol Biol (1996) 9.31

Eight prion strains have PrP(Sc) molecules with different conformations. Nat Med (1998) 9.01

Bayesian statistical analysis of protein side-chain rotamer preferences. Protein Sci (1997) 7.92

Polarization of chemoattractant receptor signaling during neutrophil chemotaxis. Science (2000) 6.93

G proteins: a family of signal transducers. Annu Rev Cell Biol (1986) 5.38

Prion propagation in mice expressing human and chimeric PrP transgenes implicates the interaction of cellular PrP with another protein. Cell (1995) 5.16

GTPase inhibiting mutations activate the alpha chain of Gs and stimulate adenylyl cyclase in human pituitary tumours. Nature (1989) 5.03

Prediction of protein side-chain rotamers from a backbone-dependent rotamer library: a new homology modeling tool. J Mol Biol (1997) 4.79

Two G protein oncogenes in human endocrine tumors. Science (1990) 4.46

Modulation of inflammation and immunity by cyclic AMP. Science (1974) 4.31

Prion protein biology. Cell (1998) 4.20

Improvements in protein secondary structure prediction by an enhanced neural network. J Mol Biol (1990) 4.10

Structure of the recombinant full-length hamster prion protein PrP(29-231): the N terminus is highly flexible. Proc Natl Acad Sci U S A (1997) 4.10

Evidence for protein X binding to a discontinuous epitope on the cellular prion protein during scrapie prion propagation. Proc Natl Acad Sci U S A (1997) 3.78

Substitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Nature (1993) 3.57

The role of cyclic 3',5' adenosine monophosphate in the specific cytolytic activity of lymphocytes. J Immunol (1972) 3.51

Spatial control of actin polymerization during neutrophil chemotaxis. Nat Cell Biol (1999) 3.45

Solution structure of a 142-residue recombinant prion protein corresponding to the infectious fragment of the scrapie isoform. Proc Natl Acad Sci U S A (1997) 3.42

Acridine and phenothiazine derivatives as pharmacotherapeutics for prion disease. Proc Natl Acad Sci U S A (2001) 3.39

Co-evolution of proteins with their interaction partners. J Mol Biol (2000) 3.23

Activation of the cloned muscarinic potassium channel by G protein beta gamma subunits. Nature (1994) 3.21

A conformational transition at the N terminus of the prion protein features in formation of the scrapie isoform. J Mol Biol (1997) 2.95

Ataxia in prion protein (PrP)-deficient mice is associated with upregulation of the novel PrP-like protein doppel. J Mol Biol (1999) 2.92

Pharmacologic control of allergic histamine release in vitro: evidence for an inhibitory role of 3',5'-adenosine monophosphate in human leukocytes. J Immunol (1972) 2.70

Hormonal stimulation of adenylyl cyclase through Gi-protein beta gamma subunits. Nature (1992) 2.62

Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis. Mol Biol Cell (1999) 2.61

Strain-specified relative conformational stability of the scrapie prion protein. Protein Sci (2001) 2.60

Leukocytes navigate by compass: roles of PI3Kgamma and its lipid products. Trends Cell Biol (2000) 2.59

Rhodopsin activation blocked by metal-ion-binding sites linking transmembrane helices C and F. Nature (1996) 2.55

Histamine augments leukocyte adenosine 3',5'-monophosphate and blocks antigenic histamine release. Science (1971) 2.47

Copper binding to the prion protein: structural implications of four identical cooperative binding sites. Proc Natl Acad Sci U S A (1999) 2.44

Prion protein gene variation among primates. J Mol Biol (1995) 2.43

ADP-ribosylation of transducin by pertussis toxin blocks the light-stimulated hydrolysis of GTP and cGMP in retinal photoreceptors. J Biol Chem (1984) 2.39

Lipid modifications of trimeric G proteins. J Biol Chem (1995) 2.39

Collecting and harvesting biological data: the GPCRDB and NucleaRDB information systems. Nucleic Acids Res (2001) 2.37

Rapid GDP release from Gs alpha in patients with gain and loss of endocrine function. Nature (1994) 2.36

Cyclic 3',5'-adenosine monophosphate in the human lukocyte: synthesis, degradation, andeffects n neutrophil candidacidal activity. J Clin Invest (1971) 2.35

Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors. Cell (1993) 2.34

Amino acid sequence of the alpha subunit of transducin deduced from the cDNA sequence. Proc Natl Acad Sci U S A (1985) 2.34

Prion protein selectively binds copper(II) ions. Biochemistry (1998) 2.32

A guanine nucleotide-sensitive adenylate cyclase in the yeast Saccharomyces cerevisiae. J Biol Chem (1983) 2.31

Activation and depalmitoylation of Gs alpha. Cell (1994) 2.29

Amino acid sequence of retinal transducin at the site ADP-ribosylated by cholera toxin. J Biol Chem (1984) 2.17

Effects of cholera enterotoxin on adenosine 3',5'-monophosphate and neutrophil function. Comparison with other compounds which stimulate leukocyte adenyl cyclase. J Clin Invest (1973) 2.16

Genetic evidence that cholera toxin substrates are regulatory components of adenylate cyclase. J Biol Chem (1978) 2.12

Recombinant scrapie-like prion protein of 106 amino acids is soluble. Proc Natl Acad Sci U S A (1996) 2.12

Separate GTP binding and GTPase activating domains of a G alpha subunit. Science (1993) 2.08

Inhibitory and stimulatory G proteins of adenylate cyclase: cDNA and amino acid sequences of the alpha chains. Proc Natl Acad Sci U S A (1986) 2.03

Identification of the Cu2+ binding sites in the N-terminal domain of the prion protein by EPR and CD spectroscopy. Biochemistry (2000) 2.02

Adenyl cyclase in human leukocytes: evidence for activation by separate beta adrenergic and prostaglandin receptors. J Pharmacol Exp Ther (1971) 2.02

Palmitoylation is required for signaling functions and membrane attachment of Gq alpha and Gs alpha. J Biol Chem (1993) 2.02

Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors. Science (1998) 2.01

Receptors induce chemotaxis by releasing the betagamma subunit of Gi, not by activating Gq or Gs. Proc Natl Acad Sci U S A (1997) 1.99

cAMP and beta gamma subunits of heterotrimeric G proteins stimulate the mitogen-activated protein kinase pathway in COS-7 cells. J Biol Chem (1994) 1.98

Hemolytic plaque formation by leukocytes in vitro. Control by vasoactive hormones. J Clin Invest (1974) 1.98

Mutations in the GTP-binding site of GS alpha alter stimulation of adenylyl cyclase. J Biol Chem (1989) 1.95

Effects of cholera toxin on in vitro models of immediate and delayed hypersensitivity. Further evidence for the role of cyclic adenosine 3',5'-monophosphate. J Clin Invest (1973) 1.94

COOH-terminal sequence of the cellular prion protein directs subcellular trafficking and controls conversion into the scrapie isoform. Proc Natl Acad Sci U S A (1997) 1.89

Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity. J Cell Biol (1992) 1.88

High-level expression and characterization of a purified 142-residue polypeptide of the prion protein. Biochemistry (1996) 1.87

Identification of a prion protein epitope modulating transmission of bovine spongiform encephalopathy prions to transgenic mice. Proc Natl Acad Sci U S A (1997) 1.87

Prion protein of 106 residues creates an artifical transmission barrier for prion replication in transgenic mice. Cell (1999) 1.87

Mutant alpha subunits of Gi2 inhibit cyclic AMP accumulation. Nature (1991) 1.87

Solution structure of Syrian hamster prion protein rPrP(90-231). Biochemistry (1999) 1.86

Folding of prion protein to its native alpha-helical conformation is under kinetic control. J Biol Chem (2001) 1.84

Four helix bundle diversity in globular proteins. J Mol Biol (1994) 1.84

Heritable disorder resembling neuronal storage disease in mice expressing prion protein with deletion of an alpha-helix. Nat Med (1997) 1.83

Selective neuronal targeting in prion disease. Neuron (1997) 1.82

Structure-based inhibitor design by using protein models for the development of antiparasitic agents. Proc Natl Acad Sci U S A (1993) 1.82

Selection of a variant lymphoma cell deficient in adenylate cyclase. Science (1975) 1.76

Family of G protein alpha chains: amphipathic analysis and predicted structure of functional domains. Protein Eng (1989) 1.76

An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells. Proc Natl Acad Sci U S A (1976) 1.74

Elimination of prions by branched polyamines and implications for therapeutics. Proc Natl Acad Sci U S A (1999) 1.73

Protein folding: evaluation of some simple rules for the assembly of helices into tertiary structures with myoglobin as an example. J Mol Biol (1979) 1.72

Receptor and betagamma binding sites in the alpha subunit of the retinal G protein transducin. Science (1997) 1.71

Sequence-specific polypeptoids: a diverse family of heteropolymers with stable secondary structure. Proc Natl Acad Sci U S A (1998) 1.70

Mutational and secondary structural analysis of the basolateral sorting signal of the polymeric immunoglobulin receptor. J Cell Biol (1993) 1.68

Defect of receptor-cyclase coupling protein in psudohypoparathyroidism. N Engl J Med (1980) 1.68

The effects of a series of prostaglandins on in vitro models of the allergic response and cellular immunity. Prostaglandins (1972) 1.67

A synthetic peptide initiates Gerstmann-Sträussler-Scheinker (GSS) disease in transgenic mice. J Mol Biol (2000) 1.65

Turn prediction in proteins using a pattern-matching approach. Biochemistry (1986) 1.65

Identification of receptor contact site involved in receptor-G protein coupling. Nature (1988) 1.65

A combinatorial approach to the discovery of efficient cationic peptoid reagents for gene delivery. Proc Natl Acad Sci U S A (1998) 1.65

Prion protein peptides induce alpha-helix to beta-sheet conformational transitions. Biochemistry (1995) 1.64

Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein. J Mol Biol (2001) 1.63

Identification of two prion protein regions that modify scrapie incubation time. J Virol (2001) 1.63

Influence of cholera toxin on the regulation of adenylate cyclase by GTP. Biochem Biophys Res Commun (1977) 1.59

A recombinant calcitonin receptor independently stimulates 3',5'-cyclic adenosine monophosphate and Ca2+/inositol phosphate signaling pathways. Mol Endocrinol (1992) 1.59

Hydrogen bonds involving sulfur atoms in proteins. Proteins (1991) 1.58

Isolation of the gene encoding adenylate cyclase in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1985) 1.58

Cholera toxin induces cAMP-independent degradation of Gs. J Biol Chem (1989) 1.57

Copper binding to octarepeat peptides of the prion protein monitored by mass spectrometry. Protein Sci (2000) 1.57

Conformational transitions in peptides containing two putative alpha-helices of the prion protein. J Mol Biol (1995) 1.57

GTP-binding proteins. One molecular machine can transduce diverse signals. Nature (1986) 1.56

In vitro antimalarial activity of chalcones and their derivatives. J Med Chem (1995) 1.56

Pertussis toxin inhibition of chemotactic factor-induced calcium mobilization and function in human polymorphonuclear leukocytes. J Exp Med (1985) 1.54

G-protein diseases furnish a model for the turn-on switch. Nature (1998) 1.54

Functional homology between signal-coupling proteins. Cholera toxin inactivates the GTPase activity of transducin. J Biol Chem (1982) 1.53