Published in Biochemistry on February 24, 1998
Multiple diverse ligands binding at a single protein site: a matter of pre-existing populations. Protein Sci (2002) 3.01
Folding and binding cascades: shifts in energy landscapes. Proc Natl Acad Sci U S A (1999) 2.37
HIV-1 protease molecular dynamics of a wild-type and of the V82F/I84V mutant: possible contributions to drug resistance and a potential new target site for drugs. Protein Sci (2004) 2.34
Rapid structural fluctuations of the free HIV protease flaps in solution: relationship to crystal structures and comparison with predictions of dynamics calculations. Protein Sci (2002) 2.17
Catalytic efficiency and vitality of HIV-1 proteases from African viral subtypes. Proc Natl Acad Sci U S A (2001) 1.64
Lack of synergy for inhibitors targeting a multi-drug-resistant HIV-1 protease. Protein Sci (2002) 1.49
Molecular basis for substrate recognition and drug resistance from 1.1 to 1.6 angstroms resolution crystal structures of HIV-1 protease mutants with substrate analogs. FEBS J (2005) 1.46
HIV-1 protease inhibitors from inverse design in the substrate envelope exhibit subnanomolar binding to drug-resistant variants. J Am Chem Soc (2008) 1.43
HIV-1 Protease: Structural Perspectives on Drug Resistance. Viruses (2009) 1.37
Resilience to resistance of HIV-1 protease inhibitors: profile of darunavir. AIDS Rev (2008) 1.26
Ligand binding and protein dynamics in lactate dehydrogenase. Biophys J (2007) 1.19
Mechanism of substrate recognition by drug-resistant human immunodeficiency virus type 1 protease variants revealed by a novel structural intermediate. J Virol (2006) 1.15
Subtype polymorphisms among HIV-1 protease variants confer altered flap conformations and flexibility. J Am Chem Soc (2009) 1.13
From nonpeptide toward noncarbon protease inhibitors: metallacarboranes as specific and potent inhibitors of HIV protease. Proc Natl Acad Sci U S A (2005) 1.11
Ninety-nine is not enough: molecular characterization of inhibitor-resistant human immunodeficiency virus type 1 protease mutants with insertions in the flap region. J Virol (2008) 1.08
Point mutations and sequence variability in proteins: redistributions of preexisting populations. Proc Natl Acad Sci U S A (2001) 1.06
Hydrophobic core flexibility modulates enzyme activity in HIV-1 protease. J Am Chem Soc (2012) 0.99
Molecular characterization of clinical isolates of human immunodeficiency virus resistant to the protease inhibitor darunavir. J Virol (2009) 0.99
Design and synthesis of HIV-1 protease inhibitors incorporating oxazolidinones as P2/P2' ligands in pseudosymmetric dipeptide isosteres. J Med Chem (2007) 0.97
Toward a universal inhibitor of retroviral proteases: comparative analysis of the interactions of LP-130 complexed with proteases from HIV-1, FIV, and EIAV. Protein Sci (1998) 0.90
Enzymatic and structural analysis of the I47A mutation contributing to the reduced susceptibility to HIV protease inhibitor lopinavir. Protein Sci (2008) 0.88
Prediction of mutational tolerance in HIV-1 protease and reverse transcriptase using flexible backbone protein design. PLoS Comput Biol (2012) 0.87
Functional correlates of insertion mutations in the protease gene of human immunodeficiency virus type 1 isolates from patients. J Virol (2001) 0.87
Structural analysis of human immunodeficiency virus type 1 CRF01_AE protease in complex with the substrate p1-p6. J Virol (2008) 0.86
Computational study of the resistance shown by the subtype B/HIV-1 protease to currently known inhibitors. Biochemistry (2010) 0.83
Tryptophan as a molecular shovel in the glycosyl transfer activity of Trypanosoma cruzi trans-sialidase. Biophys J (2010) 0.82
Effects of PRE and POST therapy drug-pressure selected mutations on HIV-1 protease conformational sampling. FEBS Lett (2014) 0.79
HIV-1 protease has a genetic T-cell adjuvant effect which is negatively regulated by proteolytic activity. J Virol (2010) 0.75
Structure of a glycerol-conducting channel and the basis for its selectivity. Science (2000) 6.64
Clq: rapid purification method for preparation of monospecific antisera and for biochemical studies. J Immunol (1971) 5.06
Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor. Proc Natl Acad Sci U S A (1984) 4.70
The signal recognition particle. Annu Rev Biochem (2001) 4.47
Structural basis of substrate specificity in the serine proteases. Protein Sci (1995) 4.22
Redesigning trypsin: alteration of substrate specificity. Science (1985) 3.70
Crystal structure of the HIV-1 integrase catalytic core and C-terminal domains: a model for viral DNA binding. Proc Natl Acad Sci U S A (2000) 3.26
Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries. Proc Natl Acad Sci U S A (2000) 3.06
Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity. Proc Natl Acad Sci U S A (1987) 2.99
Structure and function of an acetylcholine receptor. Biophys J (1982) 2.92
Efficiency of signalling through cytokine receptors depends critically on receptor orientation. Nature (1998) 2.65
Site-directed ligand discovery. Proc Natl Acad Sci U S A (2000) 2.58
Crystal structure of colicin Ia. Nature (1997) 2.57
Splice junctions: association with variation in protein structure. Science (1983) 2.54
Structure of the conserved GTPase domain of the signal recognition particle. Nature (1997) 2.52
Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates. J Biol Chem (2000) 2.50
Synthesis of positional-scanning libraries of fluorogenic peptide substrates to define the extended substrate specificity of plasmin and thrombin. Nat Biotechnol (2000) 2.49
Atomic structure of thymidylate synthase: target for rational drug design. Science (1987) 2.48
Crystal structure of the signal sequence binding subunit of the signal recognition particle. Cell (1998) 2.44
Mechanism of action of the C3b inactivator: requirement for a high molecular weight cofactor (C3b-C4bINA cofactor) and production of a new C3b derivative (C3b'). Immunochemistry (1978) 2.43
C'2ad, an inactive derivative of C'2 released during decay of EAC'4,2a. Immunochemistry (1966) 2.37
Ultrastructure of the human complement component, Clq (negative staining-glutamine synthetase-biologically active Clq). Proc Natl Acad Sci U S A (1972) 2.32
Cleavage of C2 by C1s into the antigenically distinct fragments C2a and C2b: demonstration of binding of C2b to C4b. Proc Natl Acad Sci U S A (1977) 2.27
Domain structure of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a glycoprotein of the endoplasmic reticulum. J Biol Chem (1985) 2.27
Cleavage products of C4b produced by enzymes in human serum. Immunochemistry (1975) 2.26
Regulation of an enzyme by phosphorylation at the active site. Science (1990) 2.25
Structure of two related rat pancreatic trypsin genes. J Biol Chem (1984) 2.24
Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase. Proc Natl Acad Sci U S A (1989) 2.20
Structural studies of a membrane-bound acetylcholine receptor from Torpedo californica. J Mol Biol (1977) 2.17
Rabbit C1q: purification, functional and structural studies. J Immunol Methods (1972) 2.15
Chemical studies of Clq; a modulator of immunoglobulin biology. Biochem Biophys Res Commun (1971) 2.05
Evolutionary divergence of substrate specificity within the chymotrypsin-like serine protease fold. J Biol Chem (1997) 2.05
Linking regions between helices in bacteriorhodopsin revealed. Biophys J (1982) 2.03
Reverse biochemistry: use of macromolecular protease inhibitors to dissect complex biological processes and identify a membrane-type serine protease in epithelial cancer and normal tissue. Proc Natl Acad Sci U S A (1999) 2.01
Structure, multiple site binding, and segmental accommodation in thymidylate synthase on binding dUMP and an anti-folate. Biochemistry (1990) 1.98
Structural and functional conservation between yeast and human 3-hydroxy-3-methylglutaryl coenzyme A reductases, the rate-limiting enzyme of sterol biosynthesis. Mol Cell Biol (1988) 1.97
Cleavage of C4b by C3b inactivator: production of a nicked form of C4b, C4b', as an intermediate cleavage product of C4b by C3b inactivator. J Immunol (1980) 1.94
Catalytic mechanism of NADP(+)-dependent isocitrate dehydrogenase: implications from the structures of magnesium-isocitrate and NADP+ complexes. Biochemistry (1991) 1.91
The catalytic role of the active site aspartic acid in serine proteases. Science (1987) 1.88
The three-dimensional structure of Asn102 mutant of trypsin: role of Asp102 in serine protease catalysis. Science (1987) 1.87
Structural studies on human Clq: non-covalent and covalent subunits. Immunochemistry (1972) 1.83
Structure of Cry2Aa suggests an unexpected receptor binding epitope. Structure (2001) 1.82
Immunospecific identification and three-dimensional structure of a membrane-bound acetylcholine receptor from Torpedo californica. J Mol Biol (1979) 1.82
Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel. Proc Natl Acad Sci U S A (2001) 1.80
Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs. Cell (1995) 1.80
Defining the level of human immunodeficiency virus type 1 (HIV-1) protease activity required for HIV-1 particle maturation and infectivity. J Virol (1995) 1.78
Structure at 2.5 A of a designed peptide that maintains solubility of membrane proteins. Science (1993) 1.76
Family of G protein alpha chains: amphipathic analysis and predicted structure of functional domains. Protein Eng (1989) 1.76
Molecular cloning and primary structure of human 15-lipoxygenase. Biochem Biophys Res Commun (1988) 1.76
Structure determination of asymmetric membrane profiles using an iterative Fourier method. Biophys J (1979) 1.75
Plastic adaptation toward mutations in proteins: structural comparison of thymidylate synthases. Proteins (1990) 1.75
Attachment site(s) of retinal in bacteriorhodopsin. Proc Natl Acad Sci U S A (1981) 1.73
Projected structure of purple membrane determined to 3.7 A resolution by low temperature electron microscopy. J Mol Biol (1981) 1.71
Regulation of autoproteolysis of the HIV-1 and HIV-2 proteases with engineered amino acid substitutions. J Biol Chem (1993) 1.70
Definition and redesign of the extended substrate specificity of granzyme B. J Biol Chem (1998) 1.65
Intron-exon splice junctions map at protein surfaces. Nature (1982) 1.65
Characterization of globin domains: heme binding to the central exon product. Proc Natl Acad Sci U S A (1980) 1.63
Structure and specific binding of trypsin: comparison of inhibited derivatives and a model for substrate binding. J Mol Biol (1974) 1.62
Isolation and sequence of a rat chymotrypsin B gene. J Biol Chem (1984) 1.61
Structure-based discovery of inhibitors of thymidylate synthase. Science (1993) 1.61
The sequence, organization, and expression of the major cysteine protease (cruzain) from Trypanosoma cruzi. J Biol Chem (1992) 1.58
The structure of bovine trypsin: electron density maps of the inhibited enzyme at 5 Angstrom and at 2-7 Angstron resolution. J Mol Biol (1974) 1.55
Human mast cell tryptase: multiple cDNAs and genes reveal a multigene serine protease family. Proc Natl Acad Sci U S A (1990) 1.55
A plasma kallikrein-dependent plasminogen cascade required for adipocyte differentiation. Nat Cell Biol (2001) 1.51
Mechanisms of zymogen activation. Annu Rev Biophys Bioeng (1977) 1.51
The exocrine protein trypsinogen is targeted into the secretory granules of an endocrine cell line: studies by gene transfer. J Cell Biol (1985) 1.50
The molecular neurobiology of the acetylcholine receptor. Annu Rev Neurosci (1986) 1.50
Introduction of a cysteine protease active site into trypsin. Biochemistry (1989) 1.50
The crystal structure of cruzain: a therapeutic target for Chagas' disease. J Mol Biol (1995) 1.49
Selective alteration of substrate specificity by replacement of aspartic acid-189 with lysine in the binding pocket of trypsin. Biochemistry (1987) 1.48
Functional changes in the structure of the SRP GTPase on binding GDP and Mg2+GDP. Nat Struct Biol (1999) 1.47
Structure-based design of nonpeptide inhibitors specific for the human immunodeficiency virus 1 protease. Proc Natl Acad Sci U S A (1990) 1.46
The crystal and molecular structure of DIP-inhibited bovine trypsin at2.7Angstrom resolution. Cold Spring Harb Symp Quant Biol (1972) 1.45
A primary determinant for lipoxygenase positional specificity. Nature (1991) 1.45
Structure of the two related elastase genes expressed in the rat pancreas. J Biol Chem (1984) 1.43
Epidermal growth factor (EGF) induces oligomerization of soluble, extracellular, ligand-binding domain of EGF receptor. A low resolution projection structure of the ligand-binding domain. J Biol Chem (1991) 1.41
Reversible adsorption of C'2 by EAC'4: role of Mg2+, enumeration of competent SAC'4, two-step nature of C'2a fixation and estimation of its efficiency. Immunochemistry (1966) 1.41
Two approaches to discovering and developing new drugs for Chagas disease. Mem Inst Oswaldo Cruz (2009) 1.41
The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I. Nat Struct Biol (2000) 1.41
Immunologic and proteolytic analysis of HIV-1 reverse transcriptase structure. Virology (1990) 1.38
Deficiency of C1r in human serum. Effects on the structure and function of macromolecular C1. J Exp Med (1970) 1.36
Structure of bovine trypsinogen at 1.9 A resolution. Biochemistry (1977) 1.36
Human factor D of the alternative complement pathway: purification and characterization. J Immunol (1977) 1.33
Crystal structure of human thymidylate synthase: a structural mechanism for guiding substrates into the active site. Biochemistry (1995) 1.32
Substrate specificity of trypsin investigated by using a genetic selection. Proc Natl Acad Sci U S A (1990) 1.29
Specificity and inhibition of proteases from human immunodeficiency viruses 1 and 2. J Biol Chem (1990) 1.29
The relationship of the hemolytic activity of active C'1s to its TAMe esterase action: a new method of purification and assay. J Immunol (1969) 1.28
Regulation of isocitrate dehydrogenase by phosphorylation involves no long-range conformational change in the free enzyme. J Biol Chem (1990) 1.27
Complement and host defense against infection. J Pediatr (1977) 1.26
Production of crystallizable cruzain, the major cysteine protease from Trypanosoma cruzi. J Biol Chem (1993) 1.26
The crystal structure of alpha-bungarotoxin at 2.5 A resolution: relation to solution structure and binding to acetylcholine receptor. Protein Eng (1989) 1.25
Site-directed mutagenesis shows that tyrosine 248 of carboxypeptidase A does not play a crucial role in catalysis. Nature (1985) 1.25
Viral proteases: evolution of diverse structural motifs to optimize function. Cell (1997) 1.24
Structural determinants of specificity in the cysteine protease cruzain. Protein Sci (1997) 1.24
Amplification and sequencing of genomic DNA fragments encoding cysteine proteases from protozoan parasites. Mol Biochem Parasitol (1990) 1.23
Perturbing the polar environment of Asp102 in trypsin: consequences of replacing conserved Ser214. Biochemistry (1992) 1.22
A family of protein-cutting proteins. Sci Am (1974) 1.22
Isolation and characterization of a cDNA encoding rat cationic trypsinogen. Biochemistry (1987) 1.22