Content mixing and membrane integrity during membrane fusion driven by pairing of isolated v-SNAREs and t-SNAREs.

PubWeight™: 1.95‹?› | Rank: Top 2%

🔗 View Article (PMC 22994)

Published in Proc Natl Acad Sci U S A on October 26, 1999

Authors

W Nickel1, T Weber, J A McNew, F Parlati, T H Söllner, J E Rothman

Author Affiliations

1: Cellular Biochemistry Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.

Articles citing this

Membrane fusion. Nat Struct Mol Biol (2008) 3.79

GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28. EMBO J (2000) 3.39

Rapid and efficient fusion of phospholipid vesicles by the alpha-helical core of a SNARE complex in the absence of an N-terminal regulatory domain. Proc Natl Acad Sci U S A (1999) 3.16

Close is not enough: SNARE-dependent membrane fusion requires an active mechanism that transduces force to membrane anchors. J Cell Biol (2000) 2.91

In vitro system capable of differentiating fast Ca2+-triggered content mixing from lipid exchange for mechanistic studies of neurotransmitter release. Proc Natl Acad Sci U S A (2011) 2.27

Neuronal SNAREs do not trigger fusion between synthetic membranes but do promote PEG-mediated membrane fusion. Biophys J (2005) 2.18

Reconstituted membrane fusion requires regulatory lipids, SNAREs and synergistic SNARE chaperones. EMBO J (2008) 2.16

Regulation of membrane fusion by the membrane-proximal coil of the t-SNARE during zippering of SNAREpins. J Cell Biol (2002) 2.13

Inhibition of mitochondrial fusion by α-synuclein is rescued by PINK1, Parkin and DJ-1. EMBO J (2010) 2.12

SNARE-driven, 25-millisecond vesicle fusion in vitro. Biophys J (2005) 2.08

Distinct SNARE complexes mediating membrane fusion in Golgi transport based on combinatorial specificity. Proc Natl Acad Sci U S A (2002) 2.07

SNARE-mediated lipid mixing depends on the physical state of the vesicles. Biophys J (2005) 1.91

Open syntaxin docks synaptic vesicles. PLoS Biol (2007) 1.90

Direct continuities between cisternae at different levels of the Golgi complex in glucose-stimulated mouse islet beta cells. Proc Natl Acad Sci U S A (2004) 1.86

Role of tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) in vesicular transport mediating neurite outgrowth. J Cell Biol (2000) 1.82

HOPS proofreads the trans-SNARE complex for yeast vacuole fusion. Mol Biol Cell (2008) 1.78

AtVPS45 complex formation at the trans-Golgi network. Mol Biol Cell (2000) 1.78

Synaptotagmin isoforms couple distinct ranges of Ca2+, Ba2+, and Sr2+ concentration to SNARE-mediated membrane fusion. Mol Biol Cell (2005) 1.67

Excess vacuolar SNAREs drive lysis and Rab bypass fusion. Proc Natl Acad Sci U S A (2007) 1.60

Single-molecule studies of the neuronal SNARE fusion machinery. Annu Rev Biochem (2009) 1.55

SNAREpins are functionally resistant to disruption by NSF and alphaSNAP. J Cell Biol (2000) 1.53

The abscisic acid-related SNARE homolog NtSyr1 contributes to secretion and growth: evidence from competition with its cytosolic domain. Plant Cell (2002) 1.44

A t-SNARE of the endocytic pathway must be activated for fusion. J Cell Biol (2001) 1.36

Calcium-independent stimulation of membrane fusion and SNAREpin formation by synaptotagmin I. J Cell Biol (2002) 1.33

A single vesicle-vesicle fusion assay for in vitro studies of SNAREs and accessory proteins. Nat Protoc (2012) 1.28

PEG as a tool to gain insight into membrane fusion. Eur Biophys J (2006) 1.25

Lipid mixing and content release in single-vesicle, SNARE-driven fusion assay with 1-5 ms resolution. Biophys J (2009) 1.25

A single-vesicle content mixing assay for SNARE-mediated membrane fusion. Nat Commun (2010) 1.20

Golgi-to-endoplasmic reticulum (ER) retrograde traffic in yeast requires Dsl1p, a component of the ER target site that interacts with a COPI coat subunit. Mol Biol Cell (2001) 1.19

Membrane fusion catalyzed by a Rab, SNAREs, and SNARE chaperones is accompanied by enhanced permeability to small molecules and by lysis. Mol Biol Cell (2011) 1.16

Vimentin filaments in fibroblasts are a reservoir for SNAP23, a component of the membrane fusion machinery. Mol Biol Cell (2000) 1.15

Phosphoinositides and SNARE chaperones synergistically assemble and remodel SNARE complexes for membrane fusion. Proc Natl Acad Sci U S A (2009) 1.13

Complex lipid requirements for SNARE- and SNARE chaperone-dependent membrane fusion. J Biol Chem (2009) 1.11

Intracellular bacteria encode inhibitory SNARE-like proteins. PLoS One (2009) 1.10

rsly1 binding to syntaxin 5 is required for endoplasmic reticulum-to-Golgi transport but does not promote SNARE motif accessibility. Mol Biol Cell (2003) 1.08

The identification of a novel endoplasmic reticulum to Golgi SNARE complex used by the prechylomicron transport vesicle. J Biol Chem (2006) 1.08

Physical and functional interactions of SNAP-23 with annexin A2. Am J Respir Cell Mol Biol (2007) 1.06

SNARE proteins mediate lipid bilayer fusion. Proc Natl Acad Sci U S A (1999) 1.02

Mechanical coupling via the membrane fusion SNARE protein syntaxin 1A: a molecular dynamics study. Biophys J (2003) 0.97

Complexin synchronizes primed vesicle exocytosis and regulates fusion pore dynamics. J Cell Biol (2014) 0.89

Towards reconstitution of membrane fusion mediated by SNAREs and other synaptic proteins. Crit Rev Biochem Mol Biol (2015) 0.88

Distinct contributions of vacuolar Qabc- and R-SNARE proteins to membrane fusion specificity. J Biol Chem (2011) 0.88

Atomic force microscope spectroscopy reveals a hemifusion intermediate during soluble N-ethylmaleimide-sensitive factor-attachment protein receptors-mediated membrane fusion. Biophys J (2007) 0.86

Complexin I is required for mammalian sperm acrosomal exocytosis. Dev Biol (2007) 0.86

Exocytotic fusion pores are composed of both lipids and proteins. Nat Struct Mol Biol (2015) 0.84

Single-molecule FRET study of SNARE-mediated membrane fusion. Biosci Rep (2011) 0.84

Pulling force generated by interacting SNAREs facilitates membrane hemifusion. Integr Biol (Camb) (2009) 0.84

A cascade of multiple proteins and lipids catalyzes membrane fusion. Mol Biol Cell (2017) 0.78

Investigation of SNARE-Mediated Membrane Fusion Mechanism Using Atomic Force Microscopy. Jpn J Appl Phys (2008) (2009) 0.76

A novel assay for detecting fusion pore formation: implications for the fusion mechanism. Biochemistry (2013) 0.75

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy. J Vis Exp (2016) 0.75

Rapid SNARE-mediated Fusion of Liposomes and Chromaffin Granules with Giant Unilamellar Vesicles. Biophys J (2017) 0.75

Articles cited by this

SNAP receptors implicated in vesicle targeting and fusion. Nature (1993) 18.57

SNAREpins: minimal machinery for membrane fusion. Cell (1998) 16.97

Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution. Nature (1998) 15.80

Mechanisms of intracellular protein transport. Nature (1994) 13.54

The Rab5 effector EEA1 is a core component of endosome docking. Nature (1999) 5.78

Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/deep-etch electron microscopy. Cell (1997) 5.72

A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell (1994) 4.47

Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell (1994) 4.46

Coiled coils in both intracellular vesicle and viral membrane fusion. Cell (1998) 4.26

Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins. EMBO J (1998) 3.99

The synaptic SNARE complex is a parallel four-stranded helical bundle. Nat Struct Biol (1998) 3.74

Defining the functions of trans-SNARE pairs. Nature (1998) 3.52

Multiple kinetic components of exocytosis distinguished by neurotoxin sensitivity. Nat Neurosci (1998) 3.37

Rapid and efficient fusion of phospholipid vesicles by the alpha-helical core of a SNARE complex in the absence of an N-terminal regulatory domain. Proc Natl Acad Sci U S A (1999) 3.16

SNARE complex formation is triggered by Ca2+ and drives membrane fusion. Cell (1999) 3.04

A role for giantin in docking COPI vesicles to Golgi membranes. J Cell Biol (1998) 2.84

Neurotransmitter release - four years of SNARE complexes. Curr Opin Neurobiol (1997) 2.71

The Caenorhabditis elegans unc-64 locus encodes a syntaxin that interacts genetically with synaptobrevin. Mol Biol Cell (1998) 2.70

SNAREs and NSF in targeted membrane fusion. Curr Opin Cell Biol (1997) 2.66

Structural organization of the synaptic exocytosis core complex. Neuron (1997) 2.64

p115 is a general vesicular transport factor related to the yeast endoplasmic reticulum to Golgi transport factor Uso1p. Proc Natl Acad Sci U S A (1995) 2.54

Folding intermediates of SNARE complex assembly. Nat Struct Biol (1999) 2.27

t-SNARE activation through transient interaction with a rab-like guanosine triphosphatase. Science (1997) 2.06

Regulation of SNARE complex assembly by an N-terminal domain of the t-SNARE Sso1p. Nat Struct Biol (1998) 1.91

SNAREs and membrane fusion in the Golgi apparatus. Biochim Biophys Acta (1998) 1.80

Arrangement of subunits in 20 S particles consisting of NSF, SNAPs, and SNARE complexes. Mol Cell (1998) 1.71

Genetic and morphological analyses reveal a critical interaction between the C-termini of two SNARE proteins and a parallel four helical arrangement for the exocytic SNARE complex. EMBO J (1998) 1.50

Ca2+-induced fusion of phospholipid vesicles monitored by mixing of aqueous contents. Nature (1979) 1.44

A fluorescence assay to monitor vesicle fusion and lysis. J Biol Chem (1982) 1.34

Docking and fusion in neurosecretion. Curr Opin Cell Biol (1998) 1.32

Structural and functional analysis of a novel coiled-coil protein involved in Ypt6 GTPase-regulated protein transport in yeast. Mol Biol Cell (1999) 1.19

Functional reconstitution of ypt7p GTPase and a purified vacuole SNARE complex. Science (1998) 1.09

Inhibition of membrane fusion by lysophosphatidylcholine. Biochemistry (1994) 1.05

Morphological responses to calcium-induced interaction of phosphatidylserine-containing vesicles. Biophys J (1986) 1.04

Fusion assays monitoring intermixing of aqueous contents. Methods Enzymol (1993) 0.94

Kinetic measurements of fusion of phosphatidylserine-containing vesicles by electron microscopy and fluorometry. Biochim Biophys Acta (1988) 0.90

Development of an aqueous-space mixing assay for fusion of granules and plasma membranes from human neutrophils. Biochem J (1996) 0.83

Articles by these authors

SNAP receptors implicated in vesicle targeting and fusion. Nature (1993) 18.57

SNAREpins: minimal machinery for membrane fusion. Cell (1998) 16.97

Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature (1998) 15.09

Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine. Cell (1984) 10.74

A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion. Cell (1993) 10.66

Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi. Annu Rev Biochem (1987) 10.25

Synchronised transmembrane insertion and glycosylation of a nascent membrane protein. Nature (1977) 7.72

A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast. Nature (1989) 6.48

Peptide binding and release by proteins implicated as catalysts of protein assembly. Science (1989) 5.84

Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein. Proc Natl Acad Sci U S A (1977) 5.51

Involvement of GTP-binding "G" proteins in transport through the Golgi stack. Cell (1987) 5.28

Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF. Nature (1992) 5.27

Membrane asymmetry. Science (1977) 5.12

Uncoating ATPase is a member of the 70 kilodalton family of stress proteins. Cell (1986) 4.98

The rate of bulk flow from the endoplasmic reticulum to the cell surface. Cell (1987) 4.82

ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein. Cell (1991) 4.80

Compartmental specificity of cellular membrane fusion encoded in SNARE proteins. Nature (2000) 4.67

Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack. Cell (1989) 4.65

Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport. Proc Natl Acad Sci U S A (1988) 4.63

A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell (1994) 4.47

Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack. Cell (1988) 4.37

Peptide-binding specificity of the molecular chaperone BiP. Nature (1991) 4.36

The binding of AP-1 clathrin adaptor particles to Golgi membranes requires ADP-ribosylation factor, a small GTP-binding protein. Cell (1993) 4.33

'Coatomer': a cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles. Nature (1991) 4.24

Brefeldin A, a drug that blocks secretion, prevents the assembly of non-clathrin-coated buds on Golgi cisternae. Cell (1991) 4.17

The golgi apparatus: two organelles in tandem. Science (1981) 4.15

A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein beta-adaptin. Nature (1991) 4.09

The use of pHluorins for optical measurements of presynaptic activity. Biophys J (2000) 4.07

SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeast. Cell (1990) 4.05

A new type of coated vesicular carrier that appears not to contain clathrin: its possible role in protein transport within the Golgi stack. Cell (1986) 4.04

Compartmental organization of the Golgi stack. Cell (1985) 4.02

Dissection of a single round of vesicular transport: sequential intermediates for intercisternal movement in the Golgi stack. Cell (1989) 3.78

An enzyme that removes clathrin coats: purification of an uncoating ATPase. J Cell Biol (1984) 3.77

Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein. Nature (1989) 3.62

N-ethylmaleimide-sensitive fusion protein: a trimeric ATPase whose hydrolysis of ATP is required for membrane fusion. J Cell Biol (1994) 3.59

Coated vesicles transport newly synthesized membrane glycoproteins from endoplasmic reticulum to plasma membrane in two successive stages. Proc Natl Acad Sci U S A (1980) 3.51

Characterization of protein transport between successive compartments of the Golgi apparatus: asymmetric properties of donor and acceptor activities in a cell-free system. Arch Biochem Biophys (1985) 3.43

Bidirectional transport by distinct populations of COPI-coated vesicles. Cell (1997) 3.24

Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport. Nature (1989) 3.21

Rapid and efficient fusion of phospholipid vesicles by the alpha-helical core of a SNARE complex in the absence of an N-terminal regulatory domain. Proc Natl Acad Sci U S A (1999) 3.16

Sequential intermediates in the pathway of intercompartmental transport in a cell-free system. Cell (1984) 3.07

Binding of coatomer to Golgi membranes requires ADP-ribosylation factor. J Biol Chem (1993) 3.01

Attachment of terminal N-acetylglucosamine to asparagine-linked oligosaccharides occurs in central cisternae of the Golgi stack. Cell (1985) 2.97

Close is not enough: SNARE-dependent membrane fusion requires an active mechanism that transduces force to membrane anchors. J Cell Biol (2000) 2.91

Throttles and dampers: controlling the engine of membrane fusion. Science (1997) 2.82

Stepwise assembly of functionally active transport vesicles. Cell (1993) 2.80

Bimodal interaction of coatomer with the p24 family of putative cargo receptors. Science (1996) 2.76

Sar1 promotes vesicle budding from the endoplasmic reticulum but not Golgi compartments. J Cell Biol (1994) 2.75

Regulation of protein secretion through controlled aggregation in the endoplasmic reticulum. Science (2000) 2.70

Transdermal testosterone gel improves sexual function, mood, muscle strength, and body composition parameters in hypogonadal men. J Clin Endocrinol Metab (2000) 2.68

A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack. J Cell Biol (1992) 2.67

Coated vesicle assembly in the Golgi requires only coatomer and ARF proteins from the cytosol. Nature (1993) 2.65

Compartmentation of asparagine-linked oligosaccharide processing in the Golgi apparatus. J Cell Biol (1983) 2.65

The planar organization of lecithin-cholesterol bilayers. J Biol Chem (1972) 2.62

Early and late functions associated with the Golgi apparatus reside in distinct compartments. Proc Natl Acad Sci U S A (1981) 2.61

Topological restriction of SNARE-dependent membrane fusion. Nature (2000) 2.58

Functional architecture of an intracellular membrane t-SNARE. Nature (2000) 2.57

Possible role for fatty acyl-coenzyme A in intracellular protein transport. Nature (1987) 2.56

Genetics of quantitative variation in human gene expression. Cold Spring Harb Symp Quant Biol (2003) 2.55

Transport of vesicular stomatitis virus glycoprotein in a cell-free extract. Proc Natl Acad Sci U S A (1980) 2.53

Hydrolysis of bound GTP by ARF protein triggers uncoating of Golgi-derived COP-coated vesicles. J Cell Biol (1993) 2.51

SNAP family of NSF attachment proteins includes a brain-specific isoform. Nature (1993) 2.50

Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors. Cell (1999) 2.49

A multisubunit particle implicated in membrane fusion. J Cell Biol (1992) 2.40

Detection of 14-3-3 protein in the cerebrospinal fluid supports the diagnosis of Creutzfeldt-Jakob disease. Ann Neurol (1998) 2.35

The debate about transport in the Golgi--two sides of the same coin? Cell (2000) 2.33

The amino terminus of ADP-ribosylation factor (ARF) is a critical determinant of ARF activities and is a potent and specific inhibitor of protein transport. J Biol Chem (1992) 2.31

The ATPase core of a clathrin uncoating protein. J Biol Chem (1987) 2.28

STN-DBS activates the target area in Parkinson disease: an FDG-PET study. Neurology (2008) 2.27

An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding. Proc Natl Acad Sci U S A (1995) 2.26

Receptor-mediated uptake of antigen/heat shock protein complexes results in major histocompatibility complex class I antigen presentation via two distinct processing pathways. J Exp Med (2000) 2.25

Separation of lymphocyte-stimulating and agglutinating activities in phytohaemagglutinin (PHA) from Phaseolus vulgaris. Scand J Haematol (1967) 2.22

Budding from Golgi membranes requires the coatomer complex of non-clathrin coat proteins. Nature (1993) 2.21

A new oscillometric method for pulse wave analysis: comparison with a common tonometric method. J Hum Hypertens (2010) 2.19

Ykt6p, a prenylated SNARE essential for endoplasmic reticulum-Golgi transport. J Biol Chem (1997) 2.17

Calcium-dependent switching of the specificity of phosphoinositide binding to synaptotagmin. Proc Natl Acad Sci U S A (1996) 2.16

Diagnosis of Creutzfeldt-Jakob disease by measurement of S100 protein in serum: prospective case-control study. BMJ (1998) 2.15

Enzymatic recycling of clathrin from coated vesicles. Cell (1986) 2.13

Glycosylation of a membrane protein is restricted to the growing polypeptide chain but is not necessary for insertion as a transmembrane protein. Cell (1978) 2.11