PubRank

S Tsukita

Author PubWeight™ 395.78‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 Occludin: a novel integral membrane protein localizing at tight junctions. J Cell Biol 1993 10.65
2 Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J Cell Biol 1998 7.78
3 Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. Proc Natl Acad Sci U S A 1999 5.54
4 The 102 kd cadherin-associated protein: similarity to vinculin and posttranscriptional regulation of expression. Cell 1991 5.27
5 Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association. J Cell Biol 1998 5.15
6 Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. J Cell Biol 1999 5.06
7 Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions. J Cell Biol 1994 5.01
8 Complex phenotype of mice lacking occludin, a component of tight junction strands. Mol Biol Cell 2000 4.69
9 A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts. J Cell Biol 1998 4.64
10 The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: cDNA cloning and immunoelectron microscopy. J Cell Biol 1993 4.22
11 Cadherin-mediated cell-cell adhesion is perturbed by v-src tyrosine phosphorylation in metastatic fibroblasts. J Cell Biol 1992 4.12
12 ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons. J Cell Biol 1994 4.09
13 Regulation mechanism of ERM (ezrin/radixin/moesin) protein/plasma membrane association: possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway. J Cell Biol 1996 4.06
14 Involvement of ZO-1 in cadherin-based cell adhesion through its direct binding to alpha catenin and actin filaments. J Cell Biol 1997 4.00
15 The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules. Curr Biol 2000 3.98
16 Occludin is a functional component of the tight junction. J Cell Sci 1996 3.90
17 Endothelial claudin: claudin-5/TMVCF constitutes tight junction strands in endothelial cells. J Cell Biol 1999 3.78
18 Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells. J Cell Biol 2001 3.66
19 Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctions. J Cell Biol 1998 3.62
20 Molecular linkage between cadherins and actin filaments in cell-cell adherens junctions. Curr Opin Cell Biol 1992 3.61
21 Manner of interaction of heterogeneous claudin species within and between tight junction strands. J Cell Biol 1999 3.57
22 Induction of polarized cell-cell association and retardation of growth by activation of the E-cadherin-catenin adhesion system in a dispersed carcinoma line. J Cell Biol 1994 3.43
23 Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2. J Cell Biol 1998 3.39
24 Possible involvement of phosphorylation of occludin in tight junction formation. J Cell Biol 1997 3.35
25 The roles of catenins in the cadherin-mediated cell adhesion: functional analysis of E-cadherin-alpha catenin fusion molecules. J Cell Biol 1994 3.31
26 A 220-kD undercoat-constitutive protein: its specific localization at cadherin-based cell-cell adhesion sites. J Cell Biol 1991 3.31
27 Cell-to-cell adherens junction formation and actin filament organization: similarities and differences between non-polarized fibroblasts and polarized epithelial cells. J Cell Sci 1995 3.27
28 Real-time imaging of cell-cell adherens junctions reveals that Drosophila mesoderm invagination begins with two phases of apical constriction of cells. J Cell Sci 2001 3.25
29 The FERM domain: a unique module involved in the linkage of cytoplasmic proteins to the membrane. Trends Biochem Sci 1998 3.24
30 Cytoplasmic regulation of the movement of E-cadherin on the free cell surface as studied by optical tweezers and single particle tracking: corralling and tethering by the membrane skeleton. J Cell Biol 1998 3.23
31 The movement of membranous organelles in axons. Electron microscopic identification of anterogradely and retrogradely transported organelles. J Cell Biol 1980 3.08
32 Direct interaction of the Rho GDP dissociation inhibitor with ezrin/radixin/moesin initiates the activation of the Rho small G protein. J Biol Chem 1997 3.08
33 A gene family consisting of ezrin, radixin and moesin. Its specific localization at actin filament/plasma membrane association sites. J Cell Sci 1992 3.04
34 Clostridium perfringens enterotoxin fragment removes specific claudins from tight junction strands: Evidence for direct involvement of claudins in tight junction barrier. J Cell Biol 1999 3.02
35 Occludin as a possible determinant of tight junction permeability in endothelial cells. J Cell Sci 1997 2.97
36 Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues. J Cell Biol 1996 2.94
37 Perturbation of cell adhesion and microvilli formation by antisense oligonucleotides to ERM family members. J Cell Biol 1994 2.93
38 Cadherin dysfunction in a human cancer cell line: possible involvement of loss of alpha-catenin expression in reduced cell-cell adhesiveness. Cancer Res 1992 2.89
39 Submembranous junctional plaque proteins include potential tumor suppressor molecules. J Cell Biol 1993 2.87
40 A new 82-kD barbed end-capping protein (radixin) localized in the cell-to-cell adherens junction: purification and characterization. J Cell Biol 1989 2.84
41 Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction. J Cell Biol 1997 2.82
42 Functional domains of alpha-catenin required for the strong state of cadherin-based cell adhesion. J Cell Biol 1999 2.59
43 Structural basis of the membrane-targeting and unmasking mechanisms of the radixin FERM domain. EMBO J 2000 2.49
44 Mammalian occludin in epithelial cells: its expression and subcellular distribution. Eur J Cell Biol 1997 2.46
45 Centriolar satellites: molecular characterization, ATP-dependent movement toward centrioles and possible involvement in ciliogenesis. J Cell Biol 1999 2.40
46 Radixin is a novel member of the band 4.1 family. J Cell Biol 1991 2.40
47 Claudin-11/OSP-based tight junctions of myelin sheaths in brain and Sertoli cells in testis. J Cell Biol 1999 2.40
48 Adenomatous polyposis coli (APC) protein moves along microtubules and concentrates at their growing ends in epithelial cells. J Cell Biol 2000 2.33
49 Translocation of activated Rho from the cytoplasm to membrane ruffling area, cell-cell adhesion sites and cleavage furrows. Oncogene 1995 2.32
50 Specific proto-oncogenic tyrosine kinases of src family are enriched in cell-to-cell adherens junctions where the level of tyrosine phosphorylation is elevated. J Cell Biol 1991 2.29
51 Isolation of cell-to-cell adherens junctions from rat liver. J Cell Biol 1989 2.22
52 Overexpression of occludin, a tight junction-associated integral membrane protein, induces the formation of intracellular multilamellar bodies bearing tight junction-like structures. J Cell Sci 1996 2.20
53 A truncated beta-catenin disrupts the interaction between E-cadherin and alpha-catenin: a cause of loss of intercellular adhesiveness in human cancer cell lines. Cancer Res 1994 2.17
54 Immunofluorescence detection of ezrin/radixin/moesin (ERM) proteins with their carboxyl-terminal threonine phosphorylated in cultured cells and tissues. J Cell Sci 1999 2.15
55 ERM proteins: head-to-tail regulation of actin-plasma membrane interaction. Trends Biochem Sci 1997 2.12
56 Junctional adhesion molecule (JAM) binds to PAR-3: a possible mechanism for the recruitment of PAR-3 to tight junctions. J Cell Biol 2001 2.06
57 Outer dense fiber 2 is a widespread centrosome scaffold component preferentially associated with mother centrioles: its identification from isolated centrosomes. Mol Biol Cell 2001 2.04
58 Activation of ERM proteins in vivo by Rho involves phosphatidyl-inositol 4-phosphate 5-kinase and not ROCK kinases. Curr Biol 1999 2.00
59 Tight junction-based epithelial microenvironment and cell proliferation. Oncogene 2008 1.96
60 Reduction of E-cadherin levels and deletion of the alpha-catenin gene in human prostate cancer cells. Cancer Res 1993 1.96
61 An enhanced mutant of red fluorescent protein DsRed for double labeling and developmental timer of neural fiber bundle formation. J Biol Chem 2001 1.95
62 V-src kinase shifts the cadherin-based cell adhesion from the strong to the weak state and beta catenin is not required for the shift. J Cell Biol 1995 1.92
63 Direct involvement of ezrin/radixin/moesin (ERM)-binding membrane proteins in the organization of microvilli in collaboration with activated ERM proteins. J Cell Biol 1999 1.91
64 Radixin, a barbed end-capping actin-modulating protein, is concentrated at the cleavage furrow during cytokinesis. J Cell Biol 1991 1.90
65 Two cell adhesion molecules, nectin and cadherin, interact through their cytoplasmic domain-associated proteins. J Cell Biol 2000 1.89
66 Concentration of an integral membrane protein, CD43 (leukosialin, sialophorin), in the cleavage furrow through the interaction of its cytoplasmic domain with actin-based cytoskeletons. J Cell Biol 1993 1.88
67 Epithelial transport and barrier function in occludin-deficient mice. Biochim Biophys Acta 2005 1.88
68 Dynamic behavior of the cadherin-based cell-cell adhesion system during Drosophila gastrulation. Dev Biol 1998 1.84
69 Ca(2+)-independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions. Curr Biol 1999 1.78
70 Claudin-4: a new target for pancreatic cancer treatment using Clostridium perfringens enterotoxin. Gastroenterology 2001 1.75
71 Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin. J Biol Chem 1999 1.72
72 Clostridium perfringens enterotoxin binds to the second extracellular loop of claudin-3, a tight junction integral membrane protein. FEBS Lett 2000 1.69
73 Occludin is concentrated at tight junctions of mouse/rat but not human/guinea pig Sertoli cells in testes. Am J Physiol 1998 1.68
74 Involvement of claudin-1 in the beta-catenin/Tcf signaling pathway and its frequent upregulation in human colorectal cancers. Oncol Res 2001 1.66
75 A new 400-kD protein from isolated adherens junctions: its localization at the undercoat of adherens junctions and at microfilament bundles such as stress fibers and circumferential bundles. J Cell Biol 1989 1.61
76 Regulation of tight junction permeability and occludin phosphorylation by Rhoa-p160ROCK-dependent and -independent mechanisms. J Biol Chem 2001 1.57
77 Kid, a novel kinesin-like DNA binding protein, is localized to chromosomes and the mitotic spindle. EMBO J 1996 1.57
78 Cytoskeletal network underlying the human erythrocyte membrane. Thin-section electron microscopy. J Cell Biol 1980 1.48
79 Identification of a Drosophila homologue of alpha-catenin and its association with the armadillo protein. J Cell Biol 1993 1.48
80 Elastic filaments in situ in cardiac muscle: deep-etch replica analysis in combination with selective removal of actin and myosin filaments. J Cell Biol 1993 1.46
81 Cloning of the human alpha-catenin cDNA and its aberrant mRNA in a human cancer cell line. Biochem Biophys Res Commun 1993 1.43
82 Structural diversity of band 4.1 superfamily members. J Cell Sci 1994 1.42
83 Immunohistochemical evaluation of alpha-catenin expression in human gastric cancer. Virchows Arch 1994 1.40
84 Immunohistochemical detection of alpha-catenin expression in human cancers. Am J Pathol 1994 1.40
85 Normal development of mice and unimpaired cell adhesion/cell motility/actin-based cytoskeleton without compensatory up-regulation of ezrin or radixin in moesin gene knockout. J Biol Chem 1999 1.38
86 ERM (ezrin/radixin/moesin)-based molecular mechanism of microvillar breakdown at an early stage of apoptosis. J Cell Biol 1997 1.35
87 Nonchordate classic cadherins have a structurally and functionally unique domain that is absent from chordate classic cadherins. Dev Biol 1999 1.34
88 Differential behavior of E-cadherin and occludin in their colocalization with ZO-1 during the establishment of epithelial cell polarity. J Cell Physiol 1999 1.32
89 Desmocalmin: a calmodulin-binding high molecular weight protein isolated from desmosomes. J Cell Biol 1985 1.32
90 Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion protein. FEBS Lett 1999 1.29
91 Expression of occludin, tight-junction-associated protein, in human digestive tract. Am J Pathol 1997 1.28
92 Cleavage furrow isolated from newt eggs: contraction, organization of the actin filaments, and protein components of the furrow. Proc Natl Acad Sci U S A 1988 1.25
93 Expression level, subcellular distribution and rho-GDI binding affinity of merlin in comparison with Ezrin/Radixin/Moesin proteins. Oncogene 1999 1.22
94 Frequent loss of alpha catenin expression in scirrhous carcinomas with scattered cell growth. Jpn J Cancer Res 1994 1.21
95 Actomyosin structure in contracting muscle detected by rapid freezing. Nature 1985 1.19
96 Where is APC going? J Cell Biol 2001 1.18
97 Subcellular distribution of tight junction-associated proteins (occludin, ZO-1, ZO-2) in rodent skin. J Invest Dermatol 1998 1.13
98 Dynamic features of adherens junctions during Drosophila embryonic epithelial morphogenesis revealed by a Dalpha-catenin-GFP fusion protein. Dev Genes Evol 1999 1.13
99 Bidirectional polymerization of G-actin on the human erythrocyte membrane. J Cell Biol 1984 1.12
100 Cellular actin-binding ezrin-radixin-moesin (ERM) family proteins are incorporated into the rabies virion and closely associated with viral envelope proteins in the cell. Virology 1995 1.12
101 Three-dimensional distribution of smooth endoplasmic reticulum in myelinated axons. J Electron Microsc (Tokyo) 1976 1.11
102 Transmembrane control of cadherin-mediated cell-cell adhesion. Semin Cell Biol 1993 1.10
103 The cytoskeleton in myelinated axons: a freeze-etch replica study. Neuroscience 1982 1.10
104 alpha-catenin-deficient F9 cells differentiate into signet ring cells. Am J Pathol 1999 1.06
105 Expression of E-cadherin and alpha-catenin in patients with colorectal carcinoma. Correlation with cancer invasion and metastasis. Am J Clin Pathol 1999 1.05
106 Mutations at phosphorylation sites of Xenopus microtubule-associated protein 4 affect its microtubule-binding ability and chromosome movement during mitosis. Mol Biol Cell 1999 1.03
107 Transglutaminase type 1 and its cross-linking activity are concentrated at adherens junctions in simple epithelial cells. J Biol Chem 1999 1.02
108 ATP-dependent structural changes of the outer dynein arm in Tetrahymena cilia: a freeze-etch replica study. J Cell Biol 1983 1.02
109 Synaptic vesicles contain an ATP-dependent proton pump and show 'knob-like' protrusions on their surface. EMBO J 1984 1.01
110 Subaxolemmal cytoskeleton in squid giant axon. II. Morphological identification of microtubule- and microfilament-associated domains of axolemma. J Cell Biol 1986 1.00
111 Autoimmune myocarditis induced in mice by cardiac C-protein. Cloning of complementary DNA encoding murine cardiac C-protein and partial characterization of the antigenic peptides. J Clin Invest 1994 0.99
112 A cb-type cytochrome-c oxidase terminates the respiratory chain in Helicobacter pylori. Microbiology 1996 0.98
113 Electron microscopic study of reassociation of spectrin and actin with the human erythrocyte membrane. J Cell Biol 1981 0.98
114 The undercoat of adherens junctions: a key specialized structure in organogenesis and carcinogenesis. Cell Struct Funct 1990 0.98
115 Tight junction-associated proteins (occludin, ZO-1, claudin-1, claudin-4) in squamous cell carcinoma and Bowen's disease. Br J Dermatol 2004 0.98
116 Dynamics of connexins, E-cadherin and alpha-catenin on cell membranes during gap junction formation. J Cell Sci 1997 0.97
117 Alpha-actinin from sea urchin eggs: biochemical properties, interaction with actin, and distribution in the cell during fertilization and cleavage. J Cell Biol 1985 0.97
118 Binding sites of calmodulin and actin on the brain spectrin, calspectin. J Cell Biol 1983 0.96
119 Myosin filaments in smooth muscle cells of the guinea pig taenia coli: a freeze-substitution study. Eur J Cell Biol 1982 0.95
120 Association of actin and 10 nm filaments with the dense body in smooth muscle cells of the chicken gizzard. Cell Tissue Res 1983 0.94
121 Desmoyokin, a 680 kDa keratinocyte plasma membrane-associated protein, is homologous to the protein encoded by human gene AHNAK. J Cell Sci 1993 0.94
122 Subaxolemmal cytoskeleton in squid giant axon. I. Biochemical analysis of microtubules, microfilaments, and their associated high-molecular-weight proteins. J Cell Biol 1986 0.93
123 Effects of tyrosine phosphorylation on tight junctions in temperature-sensitive v-src-transfected MDCK cells. Cell Struct Funct 1995 0.93
124 The role of ankyrin in shape and deformability change of human erythrocyte ghosts. Biochim Biophys Acta 1984 0.91
125 Localization of adherens junction proteins along the possible sliding interface between secretory ameloblasts of the rat incisor. Cell Struct Funct 1990 0.90
126 Structural conversion between open and closed forms of radixin: low-angle shadowing electron microscopy. J Mol Biol 2001 0.89
127 Mechanism of glia-neuron cell-fate switch in the Drosophila thoracic neuroblast 6-4 lineage. Development 2000 0.88
128 A new high molecular mass protein showing unique localization in desmosomal plaque. J Cell Biol 1989 0.88
129 Crystallographic characterization of the membrane-binding domain of radixin. Acta Crystallogr D Biol Crystallogr 2000 0.87
130 Structure, expression and chromosome assignment of the human catenin (cadherin-associated protein) alpha 1 gene (CTNNA1). Cytogenet Cell Genet 1994 0.86
131 Posttranscriptional regulation of alpha-catenin expression is required for Wnt signaling in L cells. Biochem Biophys Res Commun 2000 0.86
132 Characteristic structures of actin gels induced with hepatic actinogelin or with chicken gizzard alpha-actinin: implication for their function. Cell Motil Cytoskeleton 1988 0.86
133 Crystallization and preliminary crystallographic studies of RhoGDI in complex with the radixin FERM domain. Acta Crystallogr D Biol Crystallogr 2001 0.85
134 Interaction of actinogelin with actin. No nucleation but high gelation activity. Eur J Biochem 1985 0.84
135 Time-resolved electron microscopic analysis of the behavior of myosin heads on actin filaments after photolysis of caged ATP. J Cell Biol 1993 0.84
136 Morphological evidence for the involvement of the smooth endoplasmic reticulum in axonal transport. Brain Res 1979 0.84
137 Ca(2+)-regulated actin and phospholipid binding protein (68 kD-protein) from bovine liver: identification as a homologue for annexin VI and intracellular localization. Cell Motil Cytoskeleton 1992 0.83
138 Tumoricidal activity of lymphotoxin (tumor necrosis factor beta) in vivo: its effects on macrophages. J Biol Response Mod 1988 0.83
139 Expression of e-cadherin, alpha-catenins and Beta-catenins in human gastric carcinomas - correlation with histology and tumor progression. Oncol Rep 1995 0.82
140 Light-induced structural changes of cytoskeleton in squid photoreceptor microvilli detected by rapid-freeze method. J Cell Biol 1988 0.82
141 Purification and characterization of cytochrome c-553 from Helicobacter pylori. J Biochem 2000 0.82
142 Distinct mechanisms triggering glial differentiation in Drosophila thoracic and abdominal neuroblasts 6-4. Dev Biol 2000 0.81
143 Moesin is not a receptor for measles virus entry into mouse embryonic stem cells. J Virol 1998 0.81
144 The 21-kDa polypeptide (VAP21) in the rabies virion is a CD99-related host cell protein. Microbiol Immunol 1998 0.80
145 Purification and properties of dihydrogeodin oxidase from Aspergillus terreus. J Biochem 1987 0.80
146 Dynamic changes in protein components of the tight junction during liver regeneration. Cell Tissue Res 2001 0.80
147 Three-dimensional distribution of the T-system in mouse skeletal muscle. J Electron Microsc (Tokyo) 1977 0.79
148 A case of subepidermal blister disease associated with autoantibody against 450 kD protein. J Dermatol 1992 0.79
149 Regulation of microtubule organization during interphase and M phase. Cell Struct Funct 1999 0.78
150 Localization of adhesive proteins in two newly subdivided zones in electron-lucent matrix of human platelet alpha-granules. Histochemistry 1990 0.78
151 Morphology of freeze-substituted myelinated axon in mouse peripheral nerves. Brain Res 1986 0.78
152 Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail of the adhesion protein ICAM-2. Acta Crystallogr D Biol Crystallogr 2001 0.78
153 Direct correlationship between proton translocation and growth yield: an analysis of the respiratory chain of Bacillus stearothermophilus. J Biosci Bioeng 1999 0.78
154 Monomeric and trimeric structures of active Na,K-ATPase in C12E8 solution. Biochem Biophys Res Commun 1983 0.77
155 Assignment of the human alpha-catenin gene (CTNNA1) to chromosome 5q21-q22. Genomics 1994 0.77
156 Isolation of 10-nm filaments from astrocytes in the mouse optic nerve. J Cell Biol 1981 0.77
157 Co-expression of E-cadherin and alpha-catenin molecules in colorectal cancer. Surg Today 1999 0.77
158 Characterization of two terminal oxidases in Bacillus brevis and efficiency of energy conservation of the respiratory chain. J Biochem 1997 0.76
159 Possible involvement of adherens junction plaque proteins in tumorigenesis and metastasis. Princess Takamatsu Symp 1994 0.75
160 Ca2+- and Mg-ATP-dependent shape change of human erythrocyte ghosts and triton shells. Exp Cell Res 1984 0.75
161 Expression of e-cadherin and alpha-catenin molecules in human breast-cancer tissues and association with clinicopathological features. Int J Oncol 1994 0.75
162 [Filaments and microtrabeculae in axons]. Tanpakushitsu Kakusan Koso 1983 0.75
163 Assignment of the human moesin gene (MSN) to chromosome region Xq11.2-->q12. Cytogenet Cell Genet 1994 0.75
164 [Morphology in molecular cellular biology]. Tanpakushitsu Kakusan Koso 1993 0.75
165 Immunological studies of a 21 kDa cellular component efficiently incorporated into rabies virion grown in a BHK-21 cell culture. Microbiol Immunol 1997 0.75
166 A 155-kDa undercoat-constitutive protein of cell-to-cell adherens junctions. Eur J Cell Biol 1997 0.75
167 [Molecular biology of intercellular junctions]. Seikagaku 1995 0.75
168 [Adherens junction: its structure and function]. Tanpakushitsu Kakusan Koso 1989 0.75
169 [Rapid freezing technique--electron microscope]. Tanpakushitsu Kakusan Koso 1985 0.75
170 [Conformation changes in crossbridges during contraction and a possible contraction mechanism]. Tanpakushitsu Kakusan Koso 1989 0.75
171 [Axonal cytoskeleton and excitable membrane]. Tanpakushitsu Kakusan Koso 1984 0.75
172 [A case of adenocarcinoma presenting as a cavitary lesion with niveau formation]. Nihon Kyobu Shikkan Gakkai Zasshi 1993 0.75
173 The rabies virion-associated 100-kDa polypeptide (VAP100) is a host-derived minor component of the viral envelope. Microbiol Immunol 2000 0.75
174 [Axon and axolemma]. Tanpakushitsu Kakusan Koso 1990 0.75
175 Barbed end-capping protein regulates polarity of actin filaments from the human erythrocyte membrane. Exp Cell Res 1985 0.75