Published in Nature on March 13, 2003
Global rigid body modeling of macromolecular complexes against small-angle scattering data. Biophys J (2005) 7.10
Flexible fitting of atomic structures into electron microscopy maps using molecular dynamics. Structure (2008) 5.97
Protein structure fitting and refinement guided by cryo-EM density. Structure (2008) 3.12
Outcome of the First wwPDB Hybrid/Integrative Methods Task Force Workshop. Structure (2015) 2.93
Electron microscopy of whole cells in liquid with nanometer resolution. Proc Natl Acad Sci U S A (2009) 2.55
3D complex: a structural classification of protein complexes. PLoS Comput Biol (2006) 2.21
Electron microscopy of specimens in liquid. Nat Nanotechnol (2011) 2.07
Integration of small-angle X-ray scattering data into structural modeling of proteins and their assemblies. J Mol Biol (2008) 2.05
Cryo-electron microscopy of vitreous sections. EMBO J (2004) 2.03
Quantitative NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes. Proc Natl Acad Sci U S A (2006) 1.70
From quantitative microscopy to automated image understanding. J Biomed Opt (2004) 1.58
High-throughput computational and experimental techniques in structural genomics. Genome Res (2004) 1.55
The mammalian central nervous synaptic cleft contains a high density of periodically organized complexes. Proc Natl Acad Sci U S A (2005) 1.55
Spectral signal-to-noise ratio and resolution assessment of 3D reconstructions. J Struct Biol (2005) 1.41
H/D exchange and mass spectrometry in the studies of protein conformation and dynamics: is there a need for a top-down approach? Anal Chem (2009) 1.30
Modeling protein structure at near atomic resolutions with Gorgon. J Struct Biol (2011) 1.29
Interaction preferences across protein-protein interfaces of obligatory and non-obligatory components are different. BMC Struct Biol (2005) 1.27
Protein complex compositions predicted by structural similarity. Nucleic Acids Res (2006) 1.25
Ion mobility-mass spectrometry for structural proteomics. Expert Rev Proteomics (2012) 1.25
Integrating ion mobility mass spectrometry with molecular modelling to determine the architecture of multiprotein complexes. PLoS One (2010) 1.22
Segmentation of electron tomographic data sets using fuzzy set theory principles. J Struct Biol (2008) 1.21
Joint use of small-angle X-ray and neutron scattering to study biological macromolecules in solution. Eur Biophys J (2006) 1.20
Toward an integrated structural model of the 26S proteasome. Mol Cell Proteomics (2010) 1.19
Automated multi-model reconstruction from single-particle electron microscopy data. J Struct Biol (2010) 1.18
Multiple subunit fitting into a low-resolution density map of a macromolecular complex using a gaussian mixture model. Biophys J (2008) 1.17
Future directions of structural mass spectrometry using hydroxyl radical footprinting. J Mass Spectrom (2010) 1.16
Dual-color click beetle luciferase heteroprotein fragment complementation assays. Chem Biol (2010) 1.16
Thiol/disulfide redox states in signaling and sensing. Crit Rev Biochem Mol Biol (2013) 1.13
Nanoscale imaging of whole cells using a liquid enclosure and a scanning transmission electron microscope. PLoS One (2009) 1.11
Determining macromolecular assembly structures by molecular docking and fitting into an electron density map. Proteins (2010) 1.10
Identification of hot regions in protein-protein interactions by sequential pattern mining. BMC Bioinformatics (2007) 1.09
M-TASSER: an algorithm for protein quaternary structure prediction. Biophys J (2007) 1.08
Electron microscope tomography: further demonstration of nanocontacts between caveolae and smooth muscle sarcoplasmic reticulum. J Cell Mol Med (2008) 1.07
Structural modeling of protein interactions by analogy: application to PSD-95. PLoS Comput Biol (2006) 1.07
Generation of a highly inducible Gal4-->Fluc universal reporter mouse for in vivo bioluminescence imaging. Proc Natl Acad Sci U S A (2008) 1.04
Localization of protein-binding sites within families of proteins. Protein Sci (2005) 1.02
An integrated chemical cross-linking and mass spectrometry approach to study protein complex architecture and function. Mol Cell Proteomics (2011) 1.02
Evolutionary tabu search strategies for the simultaneous registration of multiple atomic structures in cryo-EM reconstructions. J Struct Biol (2010) 1.02
A voyage to the inner space of cells. Protein Sci (2005) 0.98
Exploring the spatial and temporal organization of a cell's proteome. J Struct Biol (2010) 0.97
Milestones in electron crystallography. J Comput Aided Mol Des (2006) 0.96
The role of predictive modelling in rationally re-engineering biological systems. Nat Rev Microbiol (2009) 0.96
Fully hydrated yeast cells imaged with electron microscopy. Biophys J (2011) 0.93
Modeling of protein binary complexes using structural mass spectrometry data. Protein Sci (2007) 0.93
Advances in tomography: probing the molecular architecture of cells. Nat Rev Mol Cell Biol (2012) 0.92
Constructing and validating initial Cα models from subnanometer resolution density maps with pathwalking. Structure (2012) 0.92
Uncertainty in integrative structural modeling. Curr Opin Struct Biol (2014) 0.91
Characterizing monoclonal antibody structure by carboxyl group footprinting. MAbs (2015) 0.90
Blue native polyacrylamide gel electrophoresis (BN-PAGE) for analysis of multiprotein complexes from cellular lysates. J Vis Exp (2011) 0.89
In-cell NMR spectroscopy. Prog Nucl Magn Reson Spectrosc (2010) 0.88
Three-dimensional locations of gold-labeled proteins in a whole mount eukaryotic cell obtained with 3nm precision using aberration-corrected scanning transmission electron microscopy. J Struct Biol (2011) 0.88
Combined approaches to flexible fitting and assessment in virus capsids undergoing conformational change. J Struct Biol (2013) 0.87
Protein docking by the interface structure similarity: how much structure is needed? PLoS One (2012) 0.87
Consensus among flexible fitting approaches improves the interpretation of cryo-EM data. J Struct Biol (2011) 0.86
Survey of large protein complexes in D. vulgaris reveals great structural diversity. Proc Natl Acad Sci U S A (2009) 0.86
MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map. Nucleic Acids Res (2011) 0.86
Two-photon in vivo imaging of cells. Pediatr Nephrol (2011) 0.84
Structure of frozen-hydrated triad junctions: a case study in motif searching inside tomograms. J Struct Biol (2008) 0.84
Selenium derivatization of nucleic acids for phase and structure determination in nucleic acid X-ray crystallography. Int J Mol Sci (2008) 0.84
Topological models of heteromeric protein assemblies from mass spectrometry: application to the yeast eIF3:eIF5 complex. Chem Biol (2014) 0.84
Structural interface parameters are discriminatory in recognising near-native poses of protein-protein interactions. PLoS One (2014) 0.83
Knitting and untying the protein network: modulation of protein ensembles as a therapeutic strategy. Protein Sci (2009) 0.83
The brain as a system of nested but partially overlapping networks. Heuristic relevance of the model for brain physiology and pathology. J Neural Transm (Vienna) (2006) 0.82
REACH coarse-grained normal mode analysis of protein dimer interaction dynamics. Biophys J (2009) 0.81
Micrographia of the twenty-first century: from camera obscura to 4D microscopy. Philos Trans A Math Phys Eng Sci (2010) 0.81
Direct visualization of dispersed lipid bicontinuous cubic phases by cryo-electron tomography. Nat Commun (2015) 0.81
Determining protein complex structures based on a Bayesian model of in vivo Förster resonance energy transfer (FRET) data. Mol Cell Proteomics (2014) 0.81
High-contrast observation of unstained proteins and viruses by scanning electron microscopy. PLoS One (2012) 0.79
Systems Proteomics View of the Endogenous Human Claudin Protein Family. J Proteome Res (2016) 0.79
Nanoscale imaging of untreated mammalian cells in a medium with low radiation damage using scanning electron-assisted dielectric microscopy. Sci Rep (2016) 0.78
Dynafold: a dynamic programming approach to protein backbone structure determination from minimal sets of Residual Dipolar Couplings. J Bioinform Comput Biol (2014) 0.77
Retracted Characterizing monoclonal antibody structure by carbodiimide/GEE footprinting. MAbs (2014) 0.76
Real-time shape approximation and fingerprinting of single proteins using a nanopore. Nat Nanotechnol (2016) 0.76
Traveling-wave Ion Mobility-Mass Spectrometry Reveals Additional Mechanistic Details in the Stabilization of Protein Complex Ions through Tuned Salt Additives. Int J Ion Mobil Spectrom (2013) 0.76
The STINT-NMR method for studying in-cell protein-protein interactions. Curr Protoc Protein Sci (2010) 0.76
Molecular simulation-based structural prediction of protein complexes in mass spectrometry: the human insulin dimer. PLoS Comput Biol (2014) 0.75
Bayesian Modeling of Biomolecular Assemblies with Cryo-EM Maps. Front Mol Biosci (2017) 0.75
The Croonian lecture 2006. Structure of the living cell. Philos Trans R Soc Lond B Biol Sci (2008) 0.75
Whole-cell analysis of low-density lipoprotein uptake by macrophages using STEM tomography. PLoS One (2013) 0.75
Three-dimensional X-ray observation of atmospheric biological samples by linear-array scanning-electron generation X-ray microscope system. PLoS One (2011) 0.75
Robotically assisted titration coupled to ion mobility-mass spectrometry reveals the interface structures and analysis parameters critical for multiprotein topology mapping. Anal Chem (2013) 0.75
Towards the Analysis of High Molecular Weight Proteins and Protein complexes using TIMS-MS. Int J Ion Mobil Spectrom (2016) 0.75
3D electron microscopy of biological nanomachines: principles and applications. Eur Biophys J (2007) 0.75
Structural studies by electron tomography: from cells to molecules. Annu Rev Biochem (2005) 5.56
Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science (2002) 5.28
Three-dimensional structure of herpes simplex virus from cryo-electron tomography. Science (2003) 3.82
Nuclear pore complex structure and dynamics revealed by cryoelectron tomography. Science (2004) 3.61
Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach. Proc Natl Acad Sci U S A (2012) 3.42
The molecular sociology of the cell. Nature (2007) 3.23
Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography. Proc Natl Acad Sci U S A (2005) 3.17
TOM software toolbox: acquisition and analysis for electron tomography. J Struct Biol (2005) 3.16
A polymeric protein anchors the chromosomal origin/ParB complex at a bacterial cell pole. Cell (2008) 3.15
ATP hydrolysis by the proteasome regulatory complex PAN serves multiple functions in protein degradation. Mol Cell (2003) 3.14
Snapshots of nuclear pore complexes in action captured by cryo-electron tomography. Nature (2007) 2.99
Correlative microscopy: bridging the gap between fluorescence light microscopy and cryo-electron tomography. J Struct Biol (2007) 2.29
Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Dev Cell (2002) 2.28
Structure of the 26S proteasome from Schizosaccharomyces pombe at subnanometer resolution. Proc Natl Acad Sci U S A (2010) 2.05
Near-atomic resolution structural model of the yeast 26S proteasome. Proc Natl Acad Sci U S A (2012) 2.04
Automated sample mounting and alignment system for biological crystallography at a synchrotron source. Structure (2004) 2.00
Identification of macromolecular complexes in cryoelectron tomograms of phantom cells. Proc Natl Acad Sci U S A (2002) 1.98
Three-dimensional architecture of murine rod outer segments determined by cryoelectron tomography. J Cell Biol (2007) 1.92
Visualizing cells at the nanoscale. Trends Biochem Sci (2008) 1.91
Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography. Proc Natl Acad Sci U S A (2012) 1.89
The native 3D organization of bacterial polysomes. Cell (2009) 1.88
Structural basis for recognition of acidic-cluster dileucine sequence by GGA1. Nature (2002) 1.84
3D structure of eukaryotic flagella in a quiescent state revealed by cryo-electron tomography. Proc Natl Acad Sci U S A (2005) 1.83
Insights into the molecular architecture of the 26S proteasome. Proc Natl Acad Sci U S A (2009) 1.81
A visual approach to proteomics. Nat Rev Mol Cell Biol (2006) 1.79
Cryo-electron tomography reveals the cytoskeletal structure of Spiroplasma melliferum. Science (2005) 1.72
Cryo-electron tomography: the challenge of doing structural biology in situ. J Cell Biol (2013) 1.68
Formation of cristae and crista junctions in mitochondria depends on antagonism between Fcj1 and Su e/g. J Cell Biol (2009) 1.66
Organization of actin networks in intact filopodia. Curr Biol (2007) 1.59
The proteasomal subunit Rpn6 is a molecular clamp holding the core and regulatory subcomplexes together. Proc Natl Acad Sci U S A (2011) 1.57
Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11. Proc Natl Acad Sci U S A (2014) 1.57
Structure of the 26S proteasome with ATP-γS bound provides insights into the mechanism of nucleotide-dependent substrate translocation. Proc Natl Acad Sci U S A (2013) 1.50
Micromachining tools and correlative approaches for cellular cryo-electron tomography. J Struct Biol (2010) 1.49
Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography. J Cell Biol (2010) 1.48
Cryo-electron tomography of vaccinia virus. Proc Natl Acad Sci U S A (2005) 1.48
The structure of human tripeptidyl peptidase II as determined by a hybrid approach. Structure (2012) 1.47
Noise contributions in an inducible genetic switch: a whole-cell simulation study. PLoS Comput Biol (2011) 1.44
Evidence of direct cell-cell fusion in Borrelia by cryogenic electron tomography. Cell Microbiol (2011) 1.43
Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy. Proc Natl Acad Sci U S A (2012) 1.42
Automated segmentation of electron tomograms for a quantitative description of actin filament networks. J Struct Biol (2011) 1.39
Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome. Proc Natl Acad Sci U S A (2014) 1.37
Multiscale imaging of neurons grown in culture: from light microscopy to cryo-electron tomography. J Struct Biol (2007) 1.32
Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments: implications of crowding. Biophys Chem (2003) 1.30
Mapping 70S ribosomes in intact cells by cryoelectron tomography and pattern recognition. J Struct Biol (2006) 1.28
An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome. Biochem Biophys Res Commun (2009) 1.28
The three-dimensional organization of polyribosomes in intact human cells. Mol Cell (2010) 1.26
The TB structural genomics consortium crystallization facility: towards automation from protein to electron density. Acta Crystallogr D Biol Crystallogr (2002) 1.24
20S proteasomes have the potential to keep substrates in store for continual degradation. J Biol Chem (2006) 1.20
Structural genomics of pathogenic protozoa: an overview. Methods Mol Biol (2008) 1.20
Toward an integrated structural model of the 26S proteasome. Mol Cell Proteomics (2010) 1.19
Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ. J Cell Biol (2010) 1.19
Automated cryoelectron microscopy of "single particles" applied to the 26S proteasome. FEBS Lett (2007) 1.17
The catalytic activity of Ubp6 enhances maturation of the proteasomal regulatory particle. Mol Cell (2011) 1.17
Particle picking by segmentation: a comparative study with SPIDER-based manual particle picking. J Struct Biol (2005) 1.17
Advancing cell biology through proteomics in space and time (PROSPECTS). Mol Cell Proteomics (2012) 1.15
Comparative cryo-electron tomography of pathogenic Lyme disease spirochetes. Mol Microbiol (2009) 1.14
Structural analysis of the 26S proteasome by cryoelectron tomography. Biochem Biophys Res Commun (2006) 1.13
VAT, the thermoplasma homolog of mammalian p97/VCP, is an N domain-regulated protein unfoldase. J Biol Chem (2005) 1.13
Opening windows into the cell: focused-ion-beam milling for cryo-electron tomography. Curr Opin Struct Biol (2013) 1.12
Cryo-electron tomography of cells: connecting structure and function. Histochem Cell Biol (2008) 1.12
Luminal particles within cellular microtubules. J Cell Biol (2006) 1.10
Morphological characterization of molecular complexes present in the synaptic cleft. Structure (2005) 1.06
Electron tomography of vitreous sections from cultured mammalian cells. J Struct Biol (2007) 1.06
The future is hybrid. J Struct Biol (2008) 1.05
Crystal structures of the Rhodococcus proteasome with and without its pro-peptides: implications for the role of the pro-peptide in proteasome assembly. J Mol Biol (2004) 1.05
Unveiling the long-held secrets of the 26S proteasome. Structure (2013) 1.05
Cryo-electron tomography reveals a critical role of RIM1α in synaptic vesicle tethering. J Cell Biol (2013) 1.05
Pyrodictium cannulae enter the periplasmic space but do not enter the cytoplasm, as revealed by cryo-electron tomography. J Struct Biol (2003) 1.04
Computer controlled cryo-electron microscopy--TOM² a software package for high-throughput applications. J Struct Biol (2011) 1.04
Whole cell cryo-electron tomography reveals distinct disassembly intermediates of vaccinia virus. PLoS One (2007) 1.03
Dynamic organization of the actin system in the motile cells of Dictyostelium. J Muscle Res Cell Motil (2002) 1.03
Distinct in situ structures of the Borrelia flagellar motor. J Struct Biol (2009) 1.02
Positioning of large organelles by a membrane- associated cytoskeleton in Plasmodium sporozoites. Cell Microbiol (2009) 1.02
Cryoelectron tomography reveals periodic material at the inner side of subpellicular microtubules in apicomplexan parasites. J Exp Med (2007) 1.01
The N-terminal coiled coil of the Rhodococcus erythropolis ARC AAA ATPase is neither necessary for oligomerization nor nucleotide hydrolysis. J Struct Biol (2004) 1.01
Mass spectrometry reveals the missing links in the assembly pathway of the bacterial 20 S proteasome. J Biol Chem (2007) 1.00
Maximum likelihood based classification of electron tomographic data. J Struct Biol (2010) 1.00
Extending X-ray crystallography to allow the imaging of noncrystalline materials, cells, and single protein complexes. Annu Rev Phys Chem (2008) 1.00
Localization of the regulatory particle subunit Sem1 in the 26S proteasome. Biochem Biophys Res Commun (2013) 0.96
Graphene oxide: a substrate for optimizing preparations of frozen-hydrated samples. J Struct Biol (2009) 0.96
Localization of protein complexes by pattern recognition. Methods Cell Biol (2007) 0.96
Xylanase attachment to the cell wall of the hyperthermophilic bacterium Thermotoga maritima. J Bacteriol (2007) 0.96
Quantitative proteome and transcriptome analysis of the archaeon Thermoplasma acidophilum cultured under aerobic and anaerobic conditions. J Proteome Res (2010) 0.95
Insights into the molecular organization of the neuron by cryo-electron tomography. J Electron Microsc (Tokyo) (2011) 0.94
NMR studies on the substrate-binding domains of the thermosome: structural plasticity in the protrusion region. J Mol Biol (2004) 0.94
Unraveling the structure of membrane proteins in situ by transfer function corrected cryo-electron tomography. J Struct Biol (2012) 0.94
Three-dimensional architecture of actin filaments in Listeria monocytogenes comet tails. Proc Natl Acad Sci U S A (2013) 0.92
Geometric constrains for detecting short actin filaments by cryogenic electron tomography. PMC Biophys (2010) 0.92
A giant protease with a twist: the TPP II complex from Drosophila studied by electron microscopy. EMBO J (2002) 0.92
Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements. Biochem J (2002) 0.90
Electron cryo-microscopy of VAT, the archaeal p97/CDC48 homologue from Thermoplasma acidophilum. J Mol Biol (2002) 0.88
Structure and function of tripeptidyl peptidase II, a giant cytosolic protease. Biochim Biophys Acta (2011) 0.87
Advanced beamline automation for biological crystallography experiments. Acta Crystallogr D Biol Crystallogr (2006) 0.87
Characterization of a novel intracellular endopeptidase of the alpha/beta hydrolase family from Streptomyces coelicolor A3(2). J Bacteriol (2003) 0.86
Crystal structures and proposed structural/functional classification of three protozoan proteins from the isochorismatase superfamily. Protein Sci (2005) 0.85
Hexameric ring structure of a thermophilic archaeon NADH oxidase that produces predominantly H2O. FEBS J (2008) 0.85
New insights into the structural organization of eukaryotic and prokaryotic cytoskeletons using cryo-electron tomography. Exp Cell Res (2004) 0.84
Specific orientation and two-dimensional crystallization of the proteasome at metal-chelating lipid interfaces. J Biol Chem (2002) 0.84
Structural proteomics: from the molecule to the system. Nat Struct Mol Biol (2007) 0.84
Hybrid molecular structure of the giant protease tripeptidyl peptidase II. Nat Struct Mol Biol (2010) 0.84