The virulence activator AphA links quorum sensing to pathogenesis and physiology in Vibrio cholerae by repressing the expression of a penicillin amidase gene on the small chromosome.

PubWeight™: 1.45‹?› | Rank: Top 5%

🔗 View Article (PMC 166470)

Published in J Bacteriol on August 01, 2003

Authors

Gabriela Kovacikova1, Wei Lin, Karen Skorupski

Author Affiliations

1: Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.

Articles citing this

Quorum sensing regulates type III secretion in Vibrio harveyi and Vibrio parahaemolyticus. J Bacteriol (2004) 2.55

Quorum sensing controls biofilm formation in Vibrio cholerae through modulation of cyclic di-GMP levels and repression of vpsT. J Bacteriol (2008) 2.45

Contribution of three bile-associated loci, bsh, pva, and btlB, to gastrointestinal persistence and bile tolerance of Listeria monocytogenes. Infect Immun (2005) 1.90

Genetic and phenotypic diversity of quorum-sensing systems in clinical and environmental isolates of Vibrio cholerae. Infect Immun (2006) 1.82

AphA and LuxR/HapR reciprocally control quorum sensing in vibrios. Genes Dev (2011) 1.79

Transcriptional regulation of Vibrio cholerae hemagglutinin/protease by the cyclic AMP receptor protein and RpoS. J Bacteriol (2004) 1.41

A bistable switch and anatomical site control Vibrio cholerae virulence gene expression in the intestine. PLoS Pathog (2010) 1.32

Quantification of high-specificity cyclic diguanylate signaling. Proc Natl Acad Sci U S A (2012) 1.31

Cloning, deletion, and characterization of PadR, the transcriptional repressor of the phenolic acid decarboxylase-encoding padA gene of Lactobacillus plantarum. Appl Environ Microbiol (2004) 1.30

Regulatory targets of quorum sensing in Vibrio cholerae: evidence for two distinct HapR-binding motifs. Nucleic Acids Res (2009) 1.28

Crystal structure of the virulence gene activator AphA from Vibrio cholerae reveals it is a novel member of the winged helix transcription factor superfamily. J Biol Chem (2005) 1.21

Vibrio cholerae ToxT independently activates the divergently transcribed aldA and tagA genes. J Bacteriol (2005) 1.21

QseA directly activates transcription of LEE1 in enterohemorrhagic Escherichia coli. Infect Immun (2007) 1.16

Individual and combined roles of the master regulators AphA and LuxR in control of the Vibrio harveyi quorum-sensing regulon. J Bacteriol (2012) 1.15

PhoB regulates both environmental and virulence gene expression in Vibrio cholerae. Mol Microbiol (2010) 1.11

Microarray analysis of quorum-sensing-regulated genes in Porphyromonas gingivalis. Infect Immun (2005) 1.11

Phenolic acid-mediated regulation of the padC gene, encoding the phenolic acid decarboxylase of Bacillus subtilis. J Bacteriol (2008) 1.01

Levels of the secreted Vibrio cholerae attachment factor GbpA are modulated by quorum-sensing-induced proteolysis. J Bacteriol (2009) 0.98

The virulence transcriptional activator AphA enhances biofilm formation by Vibrio cholerae by activating expression of the biofilm regulator VpsT. Infect Immun (2009) 0.98

Virulence regulator AphB enhances toxR transcription in Vibrio cholerae. BMC Microbiol (2010) 0.96

Crystal structures of two transcriptional regulators from Bacillus cereus define the conserved structural features of a PadR subfamily. PLoS One (2012) 0.96

The bile response repressor BreR regulates expression of the Vibrio cholerae breAB efflux system operon. J Bacteriol (2008) 0.95

A Vibrio cholerae relaxed (relA) mutant expresses major virulence factors, exhibits biofilm formation and motility, and colonizes the suckling mouse intestine. J Bacteriol (2006) 0.92

Inactivation of PadR, the repressor of the phenolic acid stress response, by molecular interaction with Usp1, a universal stress protein from Lactobacillus plantarum, in Escherichia coli. Appl Environ Microbiol (2009) 0.92

Genetic and biochemical analysis of PadR-padC promoter interactions during the phenolic acid stress response in Bacillus subtilis 168. J Bacteriol (2011) 0.86

Molecular characterization of direct target genes and cis-acting consensus recognized by quorum-sensing regulator AphA in Vibrio parahaemolyticus. PLoS One (2012) 0.86

(p)ppGpp, a Small Nucleotide Regulator, Directs the Metabolic Fate of Glucose in Vibrio cholerae. J Biol Chem (2015) 0.77

Parallel quorum sensing signaling pathways in Vibrio cholerae. Curr Genet (2015) 0.76

Calcium enhances bile salt-dependent virulence activation in Vibrio cholerae. Infect Immun (2016) 0.75

Transcriptional Repressor PtvR Regulates Phenotypic Tolerance to Vancomycin in Streptococcus pneumoniae. J Bacteriol (2017) 0.75

Articles cited by this

Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng (1997) 38.38

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci U S A (1988) 35.13

A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol (1988) 31.85

Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc Natl Acad Sci U S A (1987) 15.50

Lysogenic conversion by a filamentous phage encoding cholera toxin. Science (1996) 15.37

Quorum sensing in bacteria. Annu Rev Microbiol (2001) 14.41

Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene (1986) 13.92

Cholera. Clin Microbiol Rev (1995) 12.33

Cholera toxin transcriptional activator toxR is a transmembrane DNA binding protein. Cell (1987) 11.04

Regulatory cascade controls virulence in Vibrio cholerae. Proc Natl Acad Sci U S A (1991) 6.29

Quorum-sensing regulators control virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci U S A (2002) 6.10

Positive selection vectors for allelic exchange. Gene (1996) 5.97

Parallel quorum sensing systems converge to regulate virulence in Vibrio cholerae. Cell (2002) 5.12

A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains. Proc Natl Acad Sci U S A (1998) 4.75

Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 El Tor. Microbiol Immunol (1986) 4.38

TcpP protein is a positive regulator of virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci U S A (1998) 4.35

A bacteriophage encoding a pathogenicity island, a type-IV pilus and a phage receptor in cholera bacteria. Nature (1999) 3.89

Identification of toxS, a regulatory gene whose product enhances toxR-mediated activation of the cholera toxin promoter. J Bacteriol (1989) 2.87

Phase variation in tcpH modulates expression of the ToxR regulon in Vibrio cholerae. Mol Microbiol (1997) 2.71

Regulation of virulence gene expression in Vibrio cholerae by quorum sensing: HapR functions at the aphA promoter. Mol Microbiol (2002) 2.69

Cyclic AMP and its receptor protein negatively regulate the coordinate expression of cholera toxin and toxin-coregulated pilus in Vibrio cholerae. Proc Natl Acad Sci U S A (1997) 2.58

A branch in the ToxR regulatory cascade of Vibrio cholerae revealed by characterization of toxT mutant strains. Mol Microbiol (1997) 2.40

A Vibrio cholerae LysR homolog, AphB, cooperates with AphA at the tcpPH promoter to activate expression of the ToxR virulence cascade. J Bacteriol (1999) 2.33

A new level in the Vibrio cholerae ToxR virulence cascade: AphA is required for transcriptional activation of the tcpPH operon. Mol Microbiol (1999) 2.23

Regulation of gene expression in Vibrio cholerae by ToxT involves both antirepression and RNA polymerase stimulation. Mol Microbiol (2002) 2.00

The Vibrio cholerae ToxR/TcpP/ToxT virulence cascade: distinct roles for two membrane-localized transcriptional activators on a single promoter. Mol Microbiol (2000) 2.00

Overlapping binding sites for the virulence gene regulators AphA, AphB and cAMP-CRP at the Vibrio cholerae tcpPH promoter. Mol Microbiol (2001) 1.94

Vibrio cholerae H-NS silences virulence gene expression at multiple steps in the ToxR regulatory cascade. J Bacteriol (2000) 1.92

Analysis of ToxR-dependent transcription activation of ompU, the gene encoding a major envelope protein in Vibrio cholerae. Mol Microbiol (1998) 1.74

Cloning and expression of a conjugated bile acid hydrolase gene from Lactobacillus plantarum by using a direct plate assay. Appl Environ Microbiol (1992) 1.64

Molecular cloning and transcriptional regulation of ompT, a ToxR-repressed gene in Vibrio cholerae. Mol Microbiol (2000) 1.56

Inducible metabolism of phenolic acids in Pediococcus pentosaceus is encoded by an autoregulated operon which involves a new class of negative transcriptional regulator. J Bacteriol (2000) 1.35

Penicillin V acylase crystal structure reveals new Ntn-hydrolase family members. Nat Struct Biol (1999) 1.35

Mechanism of ToxT-dependent transcriptional activation at the Vibrio cholerae tcpA promoter. J Bacteriol (2002) 1.27

Differential activation of the tcpPH promoter by AphB determines biotype specificity of virulence gene expression in Vibrio cholerae. J Bacteriol (2000) 1.21

pepA, a gene mediating pH regulation of virulence genes in Vibrio cholerae. J Bacteriol (2001) 1.18

The role of penicillin amidases in nature and in industry. Trends Biochem Sci (1991) 1.13

Sequencing and heterologous expression of the gene encoding penicillin V amidase from Bacillus sphaericus. Gene (1986) 1.12

Cloning and characterization of a conjugated bile acid hydrolase gene from Clostridium perfringens. Appl Environ Microbiol (1995) 1.10

ToxR interferes with CRP-dependent transcriptional activation of ompT in Vibrio cholerae. Mol Microbiol (2002) 1.07

Binding site requirements of the virulence gene regulator AphB: differential affinities for the Vibrio cholerae classical and El Tor tcpPH promoters. Mol Microbiol (2002) 1.06

Carbon regulation and the role in nature of the Escherichia coli penicillin acylase (pac) gene. Mol Microbiol (1992) 0.98

Molecular cloning of Bacillus sphaericus penicillin V amidase gene and its expression in Escherichia coli and Bacillus subtilis. Appl Environ Microbiol (1985) 0.92

A new colorimetric method for the determination of 6-aminopenicillanic acid. Anal Biochem (1978) 0.82

Articles by these authors

A second generation human haplotype map of over 3.1 million SNPs. Nature (2007) 85.39

A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science (2002) 42.78

Landscape of transcription in human cells. Nature (2012) 20.18

Genome-wide detection and characterization of positive selection in human populations. Nature (2007) 17.27

The developmental transcriptome of Drosophila melanogaster. Nature (2010) 11.85

The Genomes of Oryza sativa: a history of duplications. PLoS Biol (2005) 7.67

A genetic variation map for chicken with 2.8 million single-nucleotide polymorphisms. Nature (2004) 5.24

Parallel quorum sensing systems converge to regulate virulence in Vibrio cholerae. Cell (2002) 5.12

An encyclopedia of mouse DNA elements (Mouse ENCODE). Genome Biol (2012) 4.15

B7-H1 blockade augments adoptive T-cell immunotherapy for squamous cell carcinoma. Cancer Res (2003) 3.96

Contextual extracellular cues promote tumor cell EMT and metastasis by regulating miR-200 family expression. Genes Dev (2009) 3.58

Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans. DNA Res (2004) 2.95

Regulation of virulence gene expression in Vibrio cholerae by quorum sensing: HapR functions at the aphA promoter. Mol Microbiol (2002) 2.69

Accurate identification of human Alu and non-Alu RNA editing sites. Nat Methods (2012) 2.47

The transcriptional diversity of 25 Drosophila cell lines. Genome Res (2010) 2.47

Foxa1 and Foxa2 regulate multiple phases of midbrain dopaminergic neuron development in a dosage-dependent manner. Development (2007) 2.40

The alternative sigma factor sigma(E) plays an important role in intestinal survival and virulence in Vibrio cholerae. Infect Immun (2002) 2.04

Dual regulation of genes involved in acetoin biosynthesis and motility/biofilm formation by the virulence activator AphA and the acetate-responsive LysR-type regulator AlsR in Vibrio cholerae. Mol Microbiol (2005) 1.78

Quantity and quality of trabecular bone in the femur are enhanced by a strongly anabolic, noninvasive mechanical intervention. J Bone Miner Res (2002) 1.68

The crystal structure of AphB, a virulence gene activator from Vibrio cholerae, reveals residues that influence its response to oxygen and pH. Mol Microbiol (2012) 1.64

Inhibitory effect of the antimalarial agent artesunate on collagen-induced arthritis in rats through nuclear factor kappa B and mitogen-activated protein kinase signaling pathway. Transl Res (2012) 1.61

Sef is a feedback-induced antagonist of Ras/MAPK-mediated FGF signalling. Nat Cell Biol (2002) 1.61

The Notch ligand Jagged2 promotes lung adenocarcinoma metastasis through a miR-200-dependent pathway in mice. J Clin Invest (2011) 1.59

Retracted Efficacy, safety, and cost of surgical versus nonsurgical treatment for carpal tunnel syndrome: A systematic review and meta-analysis. Medicine (Baltimore) (2016) 1.47

A small interfering CD147-targeting RNA inhibited the proliferation, invasiveness, and metastatic activity of malignant melanoma. Cancer Res (2006) 1.46

Structure of Vibrio cholerae ToxT reveals a mechanism for fatty acid regulation of virulence genes. Proc Natl Acad Sci U S A (2010) 1.46

Toward cloning of the magnetotactic metagenome: identification of magnetosome island gene clusters in uncultivated magnetotactic bacteria from different aquatic sediments. Appl Environ Microbiol (2009) 1.42

Expression signatures of metastatic capacity in a genetic mouse model of lung adenocarcinoma. PLoS One (2009) 1.39

Human gammadelta T lymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement. Blood (2010) 1.38

A two-stage linkage analysis of Chinese schizophrenia pedigrees in 10 target chromosomes. Biochem Biophys Res Commun (2006) 1.37

Vibrio cholerae AphA uses a novel mechanism for virulence gene activation that involves interaction with the LysR-type regulator AphB at the tcpPH promoter. Mol Microbiol (2004) 1.37

Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism. Cell Res (2010) 1.36

FcgammaRIIIa polymorphisms and cetuximab induced cytotoxicity in squamous cell carcinoma of the head and neck. Cancer Immunol Immunother (2008) 1.34

Crystal structure of the Vibrio cholerae quorum-sensing regulatory protein HapR. J Bacteriol (2007) 1.32

The LysR-type virulence activator AphB regulates the expression of genes in Vibrio cholerae in response to low pH and anaerobiosis. J Bacteriol (2010) 1.32

The quorum sensing regulator HapR downregulates the expression of the virulence gene transcription factor AphA in Vibrio cholerae by antagonizing Lrp- and VpsR-mediated activation. Mol Microbiol (2007) 1.30

The pathway of bone fluid flow as defined by in vivo intramedullary pressure and streaming potential measurements. Ann Biomed Eng (2002) 1.29

Requirements for Vibrio cholerae HapR binding and transcriptional repression at the hapR promoter are distinct from those at the aphA promoter. J Bacteriol (2005) 1.23

Characterizing IgG4-related disease with ¹⁸F-FDG PET/CT: a prospective cohort study. Eur J Nucl Med Mol Imaging (2014) 1.21

Crystal structure of the virulence gene activator AphA from Vibrio cholerae reveals it is a novel member of the winged helix transcription factor superfamily. J Biol Chem (2005) 1.21

Transcriptional repression coordinates the temporal switch from motor to serotonergic neurogenesis. Nat Neurosci (2007) 1.17

Integration host factor positively regulates virulence gene expression in Vibrio cholerae. J Bacteriol (2008) 1.14

CD137 promotes proliferation and survival of human B cells. J Immunol (2009) 1.12

Membrane trauma and Na+ leak from Nav1.6 channels. Am J Physiol Cell Physiol (2009) 1.12

Reduced expression of N-Myc downstream-regulated gene 2 in human thyroid cancer. BMC Cancer (2008) 1.11

Association of G72/G30 with schizophrenia in the Chinese population. Biochem Biophys Res Commun (2004) 1.11

Reversible superhydrophobic-superhydrophilic transition of ZnO nanorod/epoxy composite films. ACS Appl Mater Interfaces (2012) 1.10

Silver nanowires: from scalable synthesis to recyclable foldable electronics. Adv Mater (2011) 1.09

Effect of catalyst shape and etchant composition on etching direction in metal-assisted chemical etching of silicon to fabricate 3D nanostructures. ACS Nano (2009) 1.06

Binding site requirements of the virulence gene regulator AphB: differential affinities for the Vibrio cholerae classical and El Tor tcpPH promoters. Mol Microbiol (2002) 1.06

Intravitreal homocysteine-thiolactone injection leads to the degeneration of multiple retinal cells, including photoreceptors. Mol Vis (2011) 1.06

Experimental evidence for the all-up reactive conformation of chiral rhodium(II) carboxylate catalysts: enantioselective synthesis of cis-cyclopropane alpha-amino acids. J Am Chem Soc (2009) 1.06

Modification of collagen with a natural cross-linker, procyanidin. Int J Biol Macromol (2010) 1.05

Foxa1 and foxa2 are required for the maintenance of dopaminergic properties in ventral midbrain neurons at late embryonic stages. J Neurosci (2013) 1.03

Pien Tze Huang inhibits tumor cell proliferation and promotes apoptosis via suppressing the STAT3 pathway in a colorectal cancer mouse model. Int J Oncol (2012) 1.02

Detecting novel low-abundant transcripts in Drosophila. RNA (2005) 1.02

H-NS binding and repression of the ctx promoter in Vibrio cholerae. J Bacteriol (2010) 1.01

Downregulation of MSP58 inhibits growth of human colorectal cancer cells via regulation of the cyclin D1-cyclin-dependent kinase 4-p21 pathway. Cancer Sci (2009) 1.01

Histidine pairing at the metal transport site of mammalian ZnT transporters controls Zn2+ over Cd2+ selectivity. Proc Natl Acad Sci U S A (2012) 1.01

Elderly Nutrition and Health Survey in Taiwan (1999-2000): research design, methodology and content. Asia Pac J Clin Nutr (2005) 1.00

A rapid and robust numerical algorithm for sensitivity encoding with sparsity constraints: self-feeding sparse SENSE. Magn Reson Med (2010) 0.99

Levels of the secreted Vibrio cholerae attachment factor GbpA are modulated by quorum-sensing-induced proteolysis. J Bacteriol (2009) 0.98

Pt(IV) prodrugs designed to bind non-covalently to human serum albumin for drug delivery. J Am Chem Soc (2014) 0.97

Map2k4 functions as a tumor suppressor in lung adenocarcinoma and inhibits tumor cell invasion by decreasing peroxisome proliferator-activated receptor γ2 expression. Mol Cell Biol (2011) 0.96

Neuroprotective effects of osthole pretreatment against traumatic brain injury in rats. Brain Res (2011) 0.96

Sensitization of glioma cells to tamoxifen-induced apoptosis by Pl3-kinase inhibitor through the GSK-3β/β-catenin signaling pathway. PLoS One (2011) 0.96

High diversity of magnetotactic deltaproteobacteria in a freshwater niche. Appl Environ Microbiol (2013) 0.96

Gene identification and expression analysis of 86,136 Expressed Sequence Tags (EST) from the rice genome. Genomics Proteomics Bioinformatics (2003) 0.95

Cell aggregation-induced FGF8 elevation is essential for P19 cell neural differentiation. Mol Biol Cell (2006) 0.95

CDH5 is specifically activated in glioblastoma stemlike cells and contributes to vasculogenic mimicry induced by hypoxia. Neuro Oncol (2013) 0.94

Genetic, biochemical, and structural characterization of a new densovirus isolated from a chronically infected Aedes albopictus C6/36 cell line. Virology (2004) 0.94

SIRT4 regulates ATP homeostasis and mediates a retrograde signaling via AMPK. Aging (Albany NY) (2013) 0.94

Testing for the presence of magnetite in the upper-beak skin of homing pigeons. Biometals (2006) 0.94

Studying the mechanosensitivity of voltage-gated channels using oocyte patches. Methods Mol Biol (2006) 0.93

Immunohistochemical detection of Ndrg2 in the mouse nervous system. Neuroreport (2008) 0.93

Correlation between ¹⁸F-fluoromisonidazole PET and expression of HIF-1α and VEGF in newly diagnosed and recurrent malignant gliomas. Eur J Nucl Med Mol Imaging (2014) 0.92

Health-related quality of life in glioma patients in China. BMC Cancer (2010) 0.92

Isoform-specific expression of 14-3-3 proteins in human astrocytoma. J Neurol Sci (2008) 0.91

Ethyl acetate extraction from a Chinese herbal formula, Jiedu Xiaozheng Yin, inhibits the proliferation of hepatocellular carcinoma cells via induction of G0/G1 phase arrest in vivo and in vitro. Int J Oncol (2012) 0.91

Bidentate ligands on osmium(VI) nitrido complexes control intracellular targeting and cell death pathways. J Am Chem Soc (2013) 0.91

School performance, peer association, psychological and behavioral adjustments: a comparison between Chinese adolescents with and without siblings. J Adolesc (2009) 0.91

Application of image spectrometer to in situ infrared broadband optical monitoring for thin film deposition. Opt Express (2011) 0.90

A novel mutation in the RDS/Peripherin gene causes adult-onset foveomacular dystrophy. Am J Ophthalmol (2003) 0.90

Temperature induced denaturation of collagen in acidic solution. Biopolymers (2007) 0.90

Analysis of 10,000 ESTs from lymphocytes of the cynomolgus monkey to improve our understanding of its immune system. BMC Genomics (2006) 0.89

The proteasome is responsible for caspase-3-like activity during xylem development. Plant J (2012) 0.89

Association of DAAO with schizophrenia in the Chinese population. Neurosci Lett (2004) 0.89

Genome-wide characterization of Foxa2 targets reveals upregulation of floor plate genes and repression of ventrolateral genes in midbrain dopaminergic progenitors. Development (2012) 0.89

The influence of cortical end-plate on broadband ultrasound attenuation measurements at the human calcaneus using scanning confocal ultrasound. J Acoust Soc Am (2005) 0.88

Radiological diagnosis of duodenocaval fistula: a case report and literature review. World J Gastroenterol (2010) 0.88

Foxa1 and Foxa2 positively and negatively regulate Shh signalling to specify ventral midbrain progenitor identity. Mech Dev (2010) 0.88

Jiedu Xiaozheng Yin, a Chinese herbal formula, inhibits tumor angiogenesis via downregulation of VEGF-A and VEGFR-2 expression in vivo and in vitro. Oncol Rep (2012) 0.88

Spike patterning by Ca2+-dependent regulation of a muscarinic cation current in entorhinal cortex layer II neurons. J Neurophysiol (2004) 0.88