Published in J Mol Biol on February 13, 2004
A practical guide to single-molecule FRET. Nat Methods (2008) 8.62
UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke. Cell (2006) 3.65
Polyproline and the "spectroscopic ruler" revisited with single-molecule fluorescence. Proc Natl Acad Sci U S A (2005) 3.01
Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids. PLoS Biol (2007) 2.39
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A nonuniform stepping mechanism for E. coli UvrD monomer translocation along single-stranded DNA. Mol Cell (2007) 2.00
Autoinhibition of Escherichia coli Rep monomer helicase activity by its 2B subdomain. Proc Natl Acad Sci U S A (2005) 1.95
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Dynamic structural rearrangements between DNA binding modes of E. coli SSB protein. J Mol Biol (2007) 1.65
Single-molecule tracking of mRNA exiting from RNA polymerase II. Proc Natl Acad Sci U S A (2007) 1.59
Single-molecule FRET measures bends and kinks in DNA. Proc Natl Acad Sci U S A (2008) 1.58
MutS switches between two fundamentally distinct clamps during mismatch repair. Nat Struct Mol Biol (2011) 1.56
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Fast, long-range, reversible conformational fluctuations in nucleosomes revealed by single-pair fluorescence resonance energy transfer. Proc Natl Acad Sci U S A (2005) 1.38
Insights on the role of nucleic acid/protein interactions in chaperoned nucleic acid rearrangements of HIV-1 reverse transcription. Proc Natl Acad Sci U S A (2007) 1.37
Watching conformational- and photo-dynamics of single fluorescent proteins in solution. Nat Chem (2010) 1.32
Nano positioning system reveals the course of upstream and nontemplate DNA within the RNA polymerase II elongation complex. Nucleic Acids Res (2009) 1.32
Visualizing the splicing of single pre-mRNA molecules in whole cell extract. RNA (2007) 1.29
Effects of surface tethering on protein folding mechanisms. Proc Natl Acad Sci U S A (2006) 1.27
Secondary structure and secondary structure dynamics of DNA hairpins complexed with HIV-1 NC protein. Biophys J (2004) 1.26
BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation. EMBO J (2009) 1.21
Three-dimensional molecular modeling with single molecule FRET. J Struct Biol (2010) 1.20
DNA mechanics as a tool to probe helicase and translocase activity. Nucleic Acids Res (2006) 1.13
Human Rad52-mediated homology search and annealing occurs by continuous interactions between overlapping nucleoprotein complexes. Proc Natl Acad Sci U S A (2008) 1.10
Transcription initiation in a single-subunit RNA polymerase proceeds through DNA scrunching and rotation of the N-terminal subdomains. Mol Cell (2008) 1.10
Denoising single-molecule FRET trajectories with wavelets and Bayesian inference. Biophys J (2010) 1.09
Rotations of the 2B sub-domain of E. coli UvrD helicase/translocase coupled to nucleotide and DNA binding. J Mol Biol (2011) 1.04
NS3 helicase from the hepatitis C virus can function as a monomer or oligomer depending on enzyme and substrate concentrations. J Biol Chem (2008) 1.04
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Dynamic look at DNA unwinding by a replicative helicase. Proc Natl Acad Sci U S A (2014) 0.96
Förster energy transfer theory as reflected in the structures of photosynthetic light-harvesting systems. Chemphyschem (2011) 0.95
The importance of surfaces in single-molecule bioscience. Mol Biosyst (2008) 0.94
Visualizing helicases unwinding DNA at the single molecule level. Nucleic Acids Res (2010) 0.93
DNA transposition target immunity and the determinants of the MuB distribution patterns on DNA. Proc Natl Acad Sci U S A (2007) 0.90
Supertertiary structure of the synaptic MAGuK scaffold proteins is conserved. Proc Natl Acad Sci U S A (2012) 0.90
Unraveling helicase mechanisms one molecule at a time. Nucleic Acids Res (2006) 0.89
FRET-based trilateration of probes bound within functional ryanodine receptors. Biophys J (2014) 0.89
Single-molecule imaging of the oligomer formation of the nonhexameric Escherichia coli UvrD helicase. Biophys J (2013) 0.89
Reversible and controllable nanolocomotion of an RNA-processing machinery. Nano Lett (2010) 0.88
Structure and Mechanisms of SF1 DNA Helicases. Adv Exp Med Biol (2013) 0.86
Engineered holliday junctions as single-molecule reporters for protein-DNA interactions with application to a MerR-family regulator. J Am Chem Soc (2007) 0.86
A general mechanism for competitor-induced dissociation of molecular complexes. Nat Commun (2014) 0.86
Exploring protein-DNA interactions in 3D using in situ construction, manipulation and visualization of individual DNA dumbbells with optical traps, microfluidics and fluorescence microscopy. Nat Protoc (2013) 0.85
Single-molecule nanopositioning: structural transitions of a helicase-DNA complex during ATP hydrolysis. Biophys J (2011) 0.83
Mutual inhibition of RecQ molecules in DNA unwinding. J Biol Chem (2010) 0.83
Single molecule conformational analysis of DNA G-quadruplexes. Biochimie (2008) 0.82
Controlled growth of filamentous fatty acid vesicles under flow. Langmuir (2014) 0.81
Homebuilt single-molecule scanning confocal fluorescence microscope studies of single DNA/protein interactions. Methods (2007) 0.80
Taking a molecular motor for a spin: helicase mechanism studied by spin labeling and PELDOR. Nucleic Acids Res (2015) 0.80
Studying DNA-protein interactions with single-molecule Förster resonance energy transfer. Protoplasma (2013) 0.78
Relaxed rotational and scrunching changes in P266L mutant of T7 RNA polymerase reduce short abortive RNAs while delaying transition into elongation. PLoS One (2014) 0.77
Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers. Sci Rep (2016) 0.76
Designing a nine cysteine-less DNA packaging motor from bacteriophage T4 reveals new insights into ATPase structure and function. Virology (2014) 0.76
Rad51 Nucleoprotein Filament Disassembly Captured Using Fluorescent Plasmodium falciparum SSB as a Reporter for Single-Stranded DNA. PLoS One (2016) 0.75
Single-molecule studies reveal reciprocating of WRN helicase core along ssDNA during DNA unwinding. Sci Rep (2017) 0.75
Probing the ATP-induced conformational flexibility of the PcrA helicase protein using molecular dynamics simulation. J Mol Model (2016) 0.75
Using Multiorder Time-Correlation Functions (TCFs) To Elucidate Biomolecular Reaction Pathways from Microsecond Single-Molecule Fluorescence Experiments. J Phys Chem B (2016) 0.75
Single-molecule Förster resonance energy transfer studies of RNA structure, dynamics and function. Biophys Rev (2009) 0.75
Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science (2003) 20.21
A practical guide to single-molecule FRET. Nat Methods (2008) 8.62
Single-molecule high-resolution imaging with photobleaching. Proc Natl Acad Sci U S A (2004) 6.36
Nonblinking and long-lasting single-molecule fluorescence imaging. Nat Methods (2006) 6.25
Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Nature (2010) 6.25
Analysis of single-molecule FRET trajectories using hidden Markov modeling. Biophys J (2006) 5.52
Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase. Nature (2002) 5.13
Advances in single-molecule fluorescence methods for molecular biology. Annu Rev Biochem (2008) 4.40
Cytosolic viral sensor RIG-I is a 5'-triphosphate-dependent translocase on double-stranded RNA. Science (2009) 4.00
Structural dynamics of individual Holliday junctions. Nat Struct Biol (2003) 4.00
Probing cellular protein complexes using single-molecule pull-down. Nature (2011) 3.92
Repetitive shuttling of a motor protein on DNA. Nature (2005) 3.82
Spontaneous intersubunit rotation in single ribosomes. Mol Cell (2008) 3.77
Spring-loaded mechanism of DNA unwinding by hepatitis C virus NS3 helicase. Science (2007) 3.70
Probing single-stranded DNA conformational flexibility using fluorescence spectroscopy. Biophys J (2004) 3.69
RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP. Nature (2006) 3.67
Real-time observation of RecA filament dynamics with single monomer resolution. Cell (2006) 3.44
SSB as an organizer/mobilizer of genome maintenance complexes. Crit Rev Biochem Mol Biol (2008) 3.19
Near-complete suppression of quantum dot blinking in ambient conditions. J Am Chem Soc (2004) 3.14
Mechanism of ATP-dependent translocation of E.coli UvrD monomers along single-stranded DNA. J Mol Biol (2004) 2.86
A four-way junction accelerates hairpin ribozyme folding via a discrete intermediate. Proc Natl Acad Sci U S A (2003) 2.65
Multiple intermediates in SNARE-induced membrane fusion. Proc Natl Acad Sci U S A (2006) 2.65
Extreme conformational diversity in human telomeric DNA. Proc Natl Acad Sci U S A (2005) 2.63
Clonality of mouse and human cardiomyogenesis in vivo. Proc Natl Acad Sci U S A (2009) 2.60
Single-molecule three-color FRET. Biophys J (2004) 2.59
Fluorescence-force spectroscopy maps two-dimensional reaction landscape of the holliday junction. Science (2007) 2.49
Mg2+-dependent conformational change of RNA studied by fluorescence correlation and FRET on immobilized single molecules. Proc Natl Acad Sci U S A (2002) 2.47
Defining single molecular forces required to activate integrin and notch signaling. Science (2013) 2.44
Extreme bendability of DNA less than 100 base pairs long revealed by single-molecule cyclization. Science (2012) 2.42
Following movement of the L1 stalk between three functional states in single ribosomes. Proc Natl Acad Sci U S A (2009) 2.40
Vesicle encapsulation studies reveal that single molecule ribozyme heterogeneities are intrinsic. Biophys J (2004) 2.37
SSB protein diffusion on single-stranded DNA stimulates RecA filament formation. Nature (2009) 2.33
Defocused orientation and position imaging (DOPI) of myosin V. Proc Natl Acad Sci U S A (2006) 2.27
A Dimer of Escherichia coli UvrD is the active form of the helicase in vitro. J Mol Biol (2003) 2.26
Complexin and Ca2+ stimulate SNARE-mediated membrane fusion. Nat Struct Mol Biol (2008) 2.25
Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids. Proc Natl Acad Sci U S A (2008) 2.24
Roles of RIG-I N-terminal tandem CARD and splice variant in TRIM25-mediated antiviral signal transduction. Proc Natl Acad Sci U S A (2008) 2.19
Coordinating DNA replication by means of priming loop and differential synthesis rate. Nature (2009) 2.16
PcrA helicase dismantles RecA filaments by reeling in DNA in uniform steps. Cell (2010) 2.10
Early steps of supported bilayer formation probed by single vesicle fluorescence assays. Biophys J (2002) 2.09
Structural insight into brassinosteroid perception by BRI1. Nature (2011) 2.04
Crystal structure of the FTO protein reveals basis for its substrate specificity. Nature (2010) 2.04
Micromixing within microfluidic devices. Top Curr Chem (2011) 2.03
A nonuniform stepping mechanism for E. coli UvrD monomer translocation along single-stranded DNA. Mol Cell (2007) 2.00
Autoinhibition of Escherichia coli Rep monomer helicase activity by its 2B subdomain. Proc Natl Acad Sci U S A (2005) 1.95
Quantitative fluorescence labeling of aldehyde-tagged proteins for single-molecule imaging. Nat Methods (2012) 1.80
Real-time observation of strand exchange reaction with high spatiotemporal resolution. Structure (2011) 1.79
Protein induced fluorescence enhancement as a single molecule assay with short distance sensitivity. Proc Natl Acad Sci U S A (2011) 1.79
Role of laparoscopy in treatment of choledochal cysts in children. Pediatr Surg Int (2013) 1.78
Revisiting the central dogma one molecule at a time. Cell (2011) 1.75
Srs2 disassembles Rad51 filaments by a protein-protein interaction triggering ATP turnover and dissociation of Rad51 from DNA. Mol Cell (2009) 1.73
Bacillus stearothermophilus PcrA monomer is a single-stranded DNA translocase but not a processive helicase in vitro. J Biol Chem (2007) 1.71
General methods for analysis of sequential "n-step" kinetic mechanisms: application to single turnover kinetics of helicase-catalyzed DNA unwinding. Biophys J (2003) 1.70
Fueling protein DNA interactions inside porous nanocontainers. Proc Natl Acad Sci U S A (2007) 1.69
A survey of single-molecule techniques in chemical biology. ACS Chem Biol (2007) 1.68
Dynamic structural rearrangements between DNA binding modes of E. coli SSB protein. J Mol Biol (2007) 1.65
A technique for retrograde intubation in mice. Lab Anim (NY) (2006) 1.65
Coiled-coil domain-dependent homodimerization of intracellular barley immune receptors defines a minimal functional module for triggering cell death. Cell Host Microbe (2011) 1.65
Antiparallel EmrE exports drugs by exchanging between asymmetric structures. Nature (2011) 1.64
Multimodal optical sensing and analyte specificity using single-walled carbon nanotubes. Nat Nanotechnol (2008) 1.63
Dissecting metal ion-dependent folding and catalysis of a single DNAzyme. Nat Chem Biol (2007) 1.63
One influenza virus particle packages eight unique viral RNAs as shown by FISH analysis. Proc Natl Acad Sci U S A (2012) 1.61
Observing spontaneous branch migration of Holliday junctions one step at a time. Proc Natl Acad Sci U S A (2005) 1.60
Observation of internal cleavage and ligation reactions of a ribozyme. Nat Struct Mol Biol (2004) 1.58
ATPase-driven oligomerization of RIG-I on RNA allows optimal activation of type-I interferon. EMBO Rep (2013) 1.54
SSB functions as a sliding platform that migrates on DNA via reptation. Cell (2011) 1.53
Resveratrol facilitates pain attenuation in a rat model of neuropathic pain through the activation of spinal Sirt1. Reg Anesth Pain Med (2013) 1.50
Exploring rare conformational species and ionic effects in DNA Holliday junctions using single-molecule spectroscopy. J Mol Biol (2004) 1.50
Triage of pediatric injuries after the 2008 Wen-Chuan earthquake in China. J Pediatr Surg (2009) 1.50
Stopped-flow studies of the kinetics of single-stranded DNA binding and wrapping around the Escherichia coli SSB tetramer. Biochemistry (2002) 1.49
9-Butyl-9H-carbazole. Acta Crystallogr Sect E Struct Rep Online (2009) 1.47
GFP-based FRET analysis in live cells. Brain Res (2006) 1.46
ATP-dependent translocation of proteins along single-stranded DNA: models and methods of analysis of pre-steady state kinetics. J Mol Biol (2004) 1.45
DNA unwinding step-size of E. coli RecBCD helicase determined from single turnover chemical quenched-flow kinetic studies. J Mol Biol (2002) 1.44
Growth hormone improves growth in pediatric renal transplant recipients--a systemic review and meta-analysis of randomized controlled trials. Pediatr Nephrol (2012) 1.43
Interhead distance measurements in myosin VI via SHRImP support a simplified hand-over-hand model. Biophys J (2005) 1.43
Surfaces and orientations: much to FRET about? Acc Chem Res (2005) 1.42
Spectroscopic observation of RNA chaperone activities of Hfq in post-transcriptional regulation by a small non-coding RNA. Nucleic Acids Res (2007) 1.41
Single-molecule views of protein movement on single-stranded DNA. Annu Rev Biophys (2012) 1.39
Regulation of plant innate immunity by three proteins in a complex conserved across the plant and animal kingdoms. Genes Dev (2007) 1.38
Accuracy of inter-hospital paediatric appendicitis referrals. Emerg Med Australas (2012) 1.38
The 2B domain of the Escherichia coli Rep protein is not required for DNA helicase activity. Proc Natl Acad Sci U S A (2002) 1.37
Ultrahigh-resolution optical trap with single-fluorophore sensitivity. Nat Methods (2011) 1.36
MicroRNA-223 regulates FOXO1 expression and cell proliferation. FEBS Lett (2012) 1.35
The role of L1 stalk-tRNA interaction in the ribosome elongation cycle. J Mol Biol (2010) 1.35
Microsecond dynamics of protein-DNA interactions: direct observation of the wrapping/unwrapping kinetics of single-stranded DNA around the E. coli SSB tetramer. J Mol Biol (2006) 1.32
MCM forked substrate specificity involves dynamic interaction with the 5'-tail. J Biol Chem (2007) 1.32
Single-molecule analysis reveals differential effect of ssDNA-binding proteins on DNA translocation by XPD helicase. Mol Cell (2009) 1.32