A higher degree of difficulty.

A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17

RNA-guided genetic silencing systems in bacteria and archaea. Nature (2012) 17.12

Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell (2013) 13.43

CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell (2013) 9.55

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat Biotechnol (2013) 8.40

Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Science (2010) 6.79

A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotechnol (2002) 6.77

A three-dimensional view of the molecular machinery of RNA interference. Nature (2009) 6.31

Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Nat Struct Mol Biol (2011) 6.02

Structural basis for double-stranded RNA processing by Dicer. Science (2006) 5.86

Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science (2014) 5.13

Oxygen sensation and social feeding mediated by a C. elegans guanylate cyclase homologue. Nature (2004) 5.09

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions. Proc Natl Acad Sci U S A (2011) 4.58

Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation. Mol Cell (2009) 4.57

An engineered protein tag for multiprotein labeling in living cells. Chem Biol (2008) 3.92

Structural roles for human translation factor eIF3 in initiation of protein synthesis. Science (2005) 3.91

Adaptive strategies of the influenza virus polymerase for replication in humans. Proc Natl Acad Sci U S A (2009) 3.89

The RNA Ontology Consortium: an open invitation to the RNA community. RNA (2006) 3.60

Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense. Structure (2009) 3.47

In vitro reconstitution of the human RISC-loading complex. Proc Natl Acad Sci U S A (2008) 3.43

An assembly landscape for the 30S ribosomal subunit. Nature (2005) 3.33

Multiplexed RNA structure characterization with selective 2'-hydroxyl acylation analyzed by primer extension sequencing (SHAPE-Seq). Proc Natl Acad Sci U S A (2011) 3.30

Labeling of fusion proteins with synthetic fluorophores in live cells. Proc Natl Acad Sci U S A (2004) 3.11

A distributed chemosensory circuit for oxygen preference in C. elegans. PLoS Biol (2006) 2.95

Influencing receptor-ligand binding mechanisms with multivalent ligand architecture. J Am Chem Soc (2002) 2.94

Dual modes of RNA-silencing suppression by Flock House virus protein B2. Nat Struct Mol Biol (2005) 2.92

An inhibitory activity in human cells restricts the function of an avian-like influenza virus polymerase. Cell Host Microbe (2008) 2.81

Mechanism of substrate selection by a highly specific CRISPR endoribonuclease. RNA (2012) 2.78

Mechanism of foreign DNA selection in a bacterial adaptive immune system. Mol Cell (2012) 2.77

Cellobiose dehydrogenase and a copper-dependent polysaccharide monooxygenase potentiate cellulose degradation by Neurospora crassa. ACS Chem Biol (2011) 2.76

Structural determinants of RNA recognition and cleavage by Dicer. Nat Struct Mol Biol (2007) 2.67

Assembly of bacterial ribosomes. Annu Rev Biochem (2011) 2.64

Autoinhibition of human dicer by its internal helicase domain. J Mol Biol (2008) 2.62

Inter-receptor communication through arrays of bacterial chemoreceptors. Nature (2002) 2.61

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nat Chem (2013) 2.61

Thioredoxin catalyzes the S-nitrosation of the caspase-3 active site cysteine. Nat Chem Biol (2005) 2.60

Molecular mechanisms of RNA interference. Annu Rev Biophys (2013) 2.58

A conformational switch controls hepatitis delta virus ribozyme catalysis. Nature (2004) 2.57

Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases. Structure (2012) 2.51

Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3. Proc Natl Acad Sci U S A (2008) 2.50

An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3. Nat Struct Mol Biol (2011) 2.49

Mg2+-dependent conformational change of RNA studied by fluorescence correlation and FRET on immobilized single molecules. Proc Natl Acad Sci U S A (2002) 2.47

Oxidative cleavage of cellulose by fungal copper-dependent polysaccharide monooxygenases. J Am Chem Soc (2011) 2.45

The catalytic diversity of RNAs. Nat Rev Mol Cell Biol (2005) 2.42

Exploring the structural diversity of mammalian carbohydrates ("glycospace") by statistical databank analysis. ACS Chem Biol (2007) 2.42

Crystal structure of an oxygen-binding heme domain related to soluble guanylate cyclases. Proc Natl Acad Sci U S A (2004) 2.41

Trophoblast L-selectin-mediated adhesion at the maternal-fetal interface. Science (2003) 2.40

RNA target specificity of the STAR/GSG domain post-transcriptional regulatory protein GLD-1. Nat Struct Mol Biol (2003) 2.34

A molecular basis for NO selectivity in soluble guanylate cyclase. Nat Chem Biol (2005) 2.34

Synthetic GPI as a candidate anti-toxic vaccine in a model of malaria. Nature (2002) 2.31

Ribonuclease revisited: structural insights into ribonuclease III family enzymes. Curr Opin Struct Biol (2006) 2.28

Specific labeling of cell surface proteins with chemically diverse compounds. J Am Chem Soc (2004) 2.27

Precise and heritable genome editing in evolutionarily diverse nematodes using TALENs and CRISPR/Cas9 to engineer insertions and deletions. Genetics (2013) 2.22

Coordinated assembly of human translation initiation complexes by the hepatitis C virus internal ribosome entry site RNA. Proc Natl Acad Sci U S A (2004) 2.22

Oligosaccharide and glycoprotein microarrays as tools in HIV glycobiology; glycan-dependent gp120/protein interactions. Chem Biol (2004) 2.22

Structural insights into RNA processing by the human RISC-loading complex. Nat Struct Mol Biol (2009) 2.19

Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa. Proc Natl Acad Sci U S A (2009) 2.17

Selective tumor cell targeting using low-affinity, multivalent interactions. ACS Chem Biol (2007) 2.16

A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat Methods (2010) 2.15

A unique catalytic mechanism for UDP-galactopyranose mutase. Nat Struct Mol Biol (2004) 2.15

Neurons detect increases and decreases in oxygen levels using distinct guanylate cyclases. Neuron (2009) 2.14

Crystal structure of an RNA tertiary domain essential to HCV IRES-mediated translation initiation. Nat Struct Biol (2002) 2.14

Assembly of the covalent linkage between lipoic acid and its cognate enzymes. Chem Biol (2003) 2.11

Defined substrates for human embryonic stem cell growth identified from surface arrays. ACS Chem Biol (2007) 2.08

Cap-independent translation is required for starvation-induced differentiation in yeast. Science (2007) 2.05

An essential GTPase promotes assembly of preribosomal RNA processing complexes. Mol Cell (2005) 2.04

FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells. Proc Natl Acad Sci U S A (2006) 2.04

Surface plasmon resonance imaging studies of protein-carbohydrate interactions. J Am Chem Soc (2003) 1.96

Structure of human cGAS reveals a conserved family of second-messenger enzymes in innate immunity. Cell Rep (2013) 1.96

Natural Sphingomonas glycolipids vary greatly in their ability to activate natural killer T cells. Chem Biol (2008) 1.95

RNA processing enables predictable programming of gene expression. Nat Biotechnol (2012) 1.92

The use of carbohydrate microarrays to study carbohydrate-cell interactions and to detect pathogens. Chem Biol (2004) 1.91

Potentiation of fibroblast growth factor activity by synthetic heparin oligosaccharide glycodendrimers. Chem Biol (2007) 1.89

Structural and mechanistic insights into hepatitis C viral translation initiation. Nat Rev Microbiol (2006) 1.87

How to obtain labeled proteins and what to do with them. Curr Opin Biotechnol (2010) 1.85

Continuous-flow synthesis of the anti-malaria drug artemisinin. Angew Chem Int Ed Engl (2012) 1.85

Visualizing ribosome biogenesis: parallel assembly pathways for the 30S subunit. Science (2010) 1.84

N-terminal processing of proteins exported by malaria parasites. Mol Biochem Parasitol (2008) 1.81

Spectroscopic characterization of the soluble guanylate cyclase-like heme domains from Vibrio cholerae and Thermoanaerobacter tengcongensis. Biochemistry (2004) 1.80

Nitric oxide signaling: no longer simply on or off. Trends Biochem Sci (2006) 1.79

eIF3j is located in the decoding center of the human 40S ribosomal subunit. Mol Cell (2007) 1.78

Toward 'omic scale metabolite profiling: a dual separation-mass spectrometry approach for coverage of lipid and central carbon metabolism. Anal Chem (2013) 1.76

Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo. Chem Biol (2003) 1.76

Structure and regulation of soluble guanylate cyclase. Annu Rev Biochem (2012) 1.72

Structures of the complexes of a potent anti-HIV protein cyanovirin-N and high mannose oligosaccharides. J Biol Chem (2002) 1.71

The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA. Nucleic Acids Res (2009) 1.71

Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry. Mol Cell Proteomics (2007) 1.71

Quantitative proteomic analysis of ribosome assembly and turnover in vivo. J Mol Biol (2010) 1.71

Chemical probes of bacterial signal transduction reveal that repellents stabilize and attractants destabilize the chemoreceptor array. ACS Chem Biol (2008) 1.69

Synthetic GPI array to study antitoxic malaria response. Nat Chem Biol (2008) 1.67

Design and characterization of an active site selective caspase-3 transnitrosating agent. ACS Chem Biol (2006) 1.67

Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Nat Struct Mol Biol (2010) 1.66

RNA helical packing in solution: NMR structure of a 30 kDa GAAA tetraloop-receptor complex. J Mol Biol (2005) 1.65

Structure of the GLD-1 homodimerization domain: insights into STAR protein-mediated translational regulation. Structure (2010) 1.62

Direct link between RACK1 function and localization at the ribosome in vivo. Mol Cell Biol (2008) 1.61

Activating B cell signaling with defined multivalent ligands. ACS Chem Biol (2007) 1.61

Quantitative analysis of protein-RNA interactions by gel mobility shift. Methods Mol Biol (2008) 1.60

Structural insights into group II intron catalysis and branch-site selection. Science (2002) 1.58

Pathway engineered enzymatic de novo purine nucleotide synthesis. ACS Chem Biol (2008) 1.57

The j-subunit of human translation initiation factor eIF3 is required for the stable binding of eIF3 and its subcomplexes to 40 S ribosomal subunits in vitro. J Biol Chem (2003) 1.57