PubRank

Arne Skerra

Author PubWeight™ 84.11‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nat Protoc 2007 3.23
2 Crucial role for human Toll-like receptor 4 in the development of contact allergy to nickel. Nat Immunol 2010 3.15
3 Engineered protein scaffolds as next-generation antibody therapeutics. Curr Opin Chem Biol 2009 2.41
4 Interferon-gamma regulates intestinal epithelial homeostasis through converging beta-catenin signaling pathways. Immunity 2010 2.39
5 ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods 2007 2.04
6 The crystal structure of the human (S100A8/S100A9)2 heterotetramer, calprotectin, illustrates how conformational changes of interacting alpha-helices can determine specific association of two EF-hand proteins. J Mol Biol 2007 1.50
7 The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands. J Biol Chem 2004 1.30
8 Structural insights into the antigenicity of myelin oligodendrocyte glycoprotein. Proc Natl Acad Sci U S A 2003 1.27
9 The crystal structure of the bacteriophage PSA endolysin reveals a unique fold responsible for specific recognition of Listeria cell walls. J Mol Biol 2006 1.26
10 Binding proteins from alternative scaffolds. J Immunol Methods 2004 1.26
11 Temperature and pressure dependence of protein stability: the engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram. Proc Natl Acad Sci U S A 2008 1.22
12 Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody. Proc Natl Acad Sci U S A 2010 1.21
13 Comparative ligand-binding analysis of ten human lipocalins. Biochim Biophys Acta 2006 1.21
14 Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site. Protein Sci 2002 1.20
15 A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Protein Eng Des Sel 2006 1.16
16 Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture. Nat Struct Mol Biol 2004 1.13
17 Structural insight into the dual ligand specificity and mode of high density lipoprotein association of apolipoprotein D. J Biol Chem 2007 1.11
18 A new crystal form of human tear lipocalin reveals high flexibility in the loop region and induced fit in the ligand cavity. Acta Crystallogr D Biol Crystallogr 2009 1.10
19 Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells. Mol Biol Cell 2009 1.08
20 The 1.8-A crystal structure of alpha1-acid glycoprotein (Orosomucoid) solved by UV RIP reveals the broad drug-binding activity of this human plasma lipocalin. J Mol Biol 2008 1.04
21 The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site. Mol Immunol 2007 1.02
22 Structural analysis of the L-alanoyl-D-glutamate endopeptidase domain of Listeria bacteriophage endolysin Ply500 reveals a new member of the LAS peptidase family. Acta Crystallogr D Biol Crystallogr 2008 1.01
23 Lipocalins in drug discovery: from natural ligand-binding proteins to "anticalins". Drug Discov Today 2005 0.99
24 Novel serial positive enrichment technology enables clinical multiparameter cell sorting. PLoS One 2012 0.98
25 Neutrophil gelatinase-associated lipocalin expresses antimicrobial activity by interfering with L-norepinephrine-mediated bacterial iron acquisition. Antimicrob Agents Chemother 2010 0.97
26 Structural mechanism of specific ligand recognition by a lipocalin tailored for the complexation of digoxigenin. J Mol Biol 2003 0.97
27 Alternative binding proteins get mature: rivalling antibodies. FEBS J 2008 0.97
28 PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins. Protein Eng Des Sel 2013 0.97
29 Crystallographic analysis of an "anticalin" with tailored specificity for fluorescein reveals high structural plasticity of the lipocalin loop region. Proteins 2003 0.96
30 Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: effects on biophysical properties and prolonged plasma half-life. Protein Eng Des Sel 2007 0.96
31 Comparative analysis reveals selective recognition of glycans by the dendritic cell receptors DC-SIGN and Langerin. Protein Eng Des Sel 2011 0.95
32 Identification of potential substrate proteins for the periplasmic Escherichia coli chaperone Skp. Proteomics 2008 0.95
33 New insights into the signaling mechanism of the pH-responsive, membrane-integrated transcriptional activator CadC of Escherichia coli. J Biol Chem 2011 0.93
34 Anticalins small engineered binding proteins based on the lipocalin scaffold. Methods Enzymol 2012 0.92
35 Construction of an artificial receptor protein ("anticalin") based on the human apolipoprotein D. Chembiochem 2004 0.90
36 The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops. Proteins 2008 0.90
37 Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism. Acta Crystallogr Sect F Struct Biol Cryst Commun 2009 0.89
38 Solubility engineering and crystallization of human apolipoprotein D. Protein Sci 2005 0.88
39 A disulfide-free single-domain V(L) intrabody with blocking activity towards huntingtin reveals a novel mode of epitope recognition. J Mol Biol 2011 0.88
40 Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach. Biophys Chem 2002 0.87
41 Extra-domain B in oncofetal fibronectin structurally promotes fibrillar head-to-tail dimerization of extracellular matrix protein. J Biol Chem 2012 0.87
42 Functional humanization of an anti-CD30 Fab fragment for the immunotherapy of Hodgkin's lymphoma using an in vitro evolution approach. Protein Eng Des Sel 2005 0.86
43 Rational engineering of a fluorescein-binding anticalin for improved ligand affinity. Biol Chem 2005 0.85
44 Biochemical analysis of recombinant AlkJ from Pseudomonas putida reveals a membrane-associated, flavin adenine dinucleotide-dependent dehydrogenase suitable for the biosynthetic production of aliphatic aldehydes. Appl Environ Microbiol 2014 0.84
45 Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family. Protein Sci 2011 0.84
46 Crystal structure of the ω-aminotransferase from Paracoccus denitrificans and its phylogenetic relationship with other class III aminotransferases that have biotechnological potential. Proteins 2013 0.84
47 The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization. Biol Chem 2004 0.84
48 Structure of lumazine protein, an optical transponder of luminescent bacteria. J Mol Biol 2008 0.83
49 Endoplasmic reticulum stress response promotes cytotoxic phenotype of CD8αβ+ intraepithelial lymphocytes in a mouse model for Crohn's disease-like ileitis. J Immunol 2012 0.83
50 Crystallographic analysis and structure-guided engineering of NADPH-dependent Ralstonia sp. alcohol dehydrogenase toward NADH cosubstrate specificity. Biotechnol Bioeng 2013 0.82
51 A photoswitchable neurotransmitter analogue bound to its receptor. Biochemistry 2013 0.82
52 Structural basis for the enzymatic formation of the key strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone. J Biol Chem 2013 0.82
53 Anticalins as an alternative to antibody technology. Expert Opin Biol Ther 2005 0.82
54 High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface. J Mol Biol 2010 0.82
55 Biochemical characterization of the recombinant human Nogo-A ectodomain. FEBS J 2007 0.82
56 Functional humanization of an anti-CD16 Fab fragment: obstacles of switching from murine {lambda} to human {lambda} or {kappa} light chains. Protein Eng Des Sel 2008 0.81
57 A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of F(ab) fragments and leads to improved recombinant immunoglobulin stability. Biosens Bioelectron 2002 0.81
58 High-affinity recognition of lanthanide(III) chelate complexes by a reprogrammed human lipocalin 2. J Am Chem Soc 2009 0.81
59 Bacterial production and functional characterization of the Fab fragment of the murine IgG1/lambda monoclonal antibody cmHsp70.1, a reagent for tumour diagnostics. Protein Eng Des Sel 2010 0.81
60 Combinatorial design of an Anticalin directed against the extra-domain b for the specific targeting of oncofetal fibronectin. J Mol Biol 2012 0.80
61 Deactivation of the E. coli pH stress sensor CadC by cadaverine. J Mol Biol 2012 0.80
62 NMR structure and dynamics of the engineered fluorescein-binding lipocalin FluA reveal rigidification of beta-barrel and variable loops upon enthalpy-driven ligand binding. Biochemistry 2009 0.79
63 Epitope mapping of the neuronal growth inhibitor Nogo-A for the Nogo receptor and the cognate monoclonal antibody IN-1 by means of the SPOT technique. J Mol Recognit 2007 0.79
64 Periplasmic chaperones used to enhance functional secretion of proteins in E. coli. Methods Mol Biol 2011 0.79
65 β-Barrel scaffolds for the grafting of extracellular loops from G-protein-coupled receptors. Biol Chem 2012 0.78
66 Anticalins: the lipocalin family as a novel protein scaffold for the development of next-generation immunotherapies. Expert Rev Clin Immunol 2007 0.78
67 An expression system for the E. coli fermentation of recombinant antibody Fab fragments from mice and rabbits. J AOAC Int 2010 0.77
68 NMR structure of lipoprotein YxeF from Bacillus subtilis reveals a calycin fold and distant homology with the lipocalin Blc from Escherichia coli. PLoS One 2012 0.77
69 The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture. Methods Mol Biol 2015 0.77
70 Design and characterization of a modular membrane protein anchor to functionalize the moss Physcomitrella patens with extracellular catalytic and/or binding activities. ACS Synth Biol 2014 0.77
71 Intravenous application of an anticalin dramatically lowers plasma digoxin levels and reduces its toxic effects in rats. Toxicol Appl Pharmacol 2012 0.77
72 A novel method for the N-terminal modification of native proteins. Bioconjug Chem 2004 0.77
73 Resonance assignments for the 21 kDa engineered fluorescein-binding lipocalin FluA. J Biomol NMR 2003 0.77
74 The crystal structure of human α(1)-microglobulin reveals a potential haem-binding site. Biochem J 2012 0.77
75 Structural and biochemical analyses reveal a monomeric state of the bacterial lipocalin Blc. Acta Crystallogr D Biol Crystallogr 2010 0.77
76 DNA vaccination efficiently induces antibodies to Nogo-A and does not exacerbate experimental autoimmune encephalomyelitis. Eur J Pharmacol 2008 0.77
77 Biosynthesis of a fluorescent protein with extreme pseudo-Stokes shift by introducing a genetically encoded non-natural amino acid outside the fluorophore. J Am Chem Soc 2011 0.76
78 High contrast tumor imaging with radio-labeled antibody Fab fragments tailored for optimized pharmacokinetics via PASylation. MAbs 2015 0.76
79 Generation of anticalins with specificity for a nonsymmetric phthalic acid ester. Anal Biochem 2002 0.76
80 Detection of irradiation-induced, membrane heat shock protein 70 (Hsp70) in mouse tumors using Hsp70 Fab fragment. Radiother Oncol 2011 0.76
81 High molecular mass radioimmunoconjugates are promising for intraperitoneal α-emitter immunotherapy due to prolonged retention in the peritoneum. Nucl Med Biol 2012 0.76
82 PAS-cal: a generic recombinant peptide calibration standard for mass spectrometry. J Am Soc Mass Spectrom 2014 0.75
83 Derivatization of antibody Fab fragments: a designer enzyme for native protein modification. Chembiochem 2014 0.75
84 A system for repertoire cloning and phage display of murine and leporid antibody fragments. J AOAC Int 2010 0.75
85 PAS-cal: A repetitive peptide sequence calibration standard for MALDI mass spectrometry. Proteomics 2014 0.75
86 Anticalins directed against vascular endothelial growth factor receptor 3 (VEGFR-3) with picomolar affinities show potential for medical therapy and in vivo imaging. Biol Chem 2017 0.75
87 Anticalins in drug development. BioDrugs 2005 0.75
88 Engineering of an orthogonal aminoacyl-tRNA synthetase for efficient incorporation of the non-natural amino acid O-methyl-L-tyrosine using fluorescence-based bacterial cell sorting. J Mol Biol 2010 0.75
89 A HLA-Cw6 specific single-chain antibody fragment (scFv) recognizing a natural killer cell receptor epitope. Mol Immunol 2005 0.75