Lysosomes as "suicide bags" in cell death: myth or reality?

PubWeight™: 1.65‹?› | Rank: Top 3%

🔗 View Article (PMC 2755904)

Published in J Biol Chem on May 27, 2009

Authors

Boris Turk1, Vito Turk

Author Affiliations

1: Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia. boris.turk@ijs.si

Articles citing this

Reversal of autophagy dysfunction in the TgCRND8 mouse model of Alzheimer's disease ameliorates amyloid pathologies and memory deficits. Brain (2011) 2.76

Autophagy in protists. Autophagy (2011) 1.29

Protease signalling: the cutting edge. EMBO J (2012) 1.24

Proteolysis mediated by cysteine cathepsins and legumain-recent advances and cell biological challenges. Protoplasma (2014) 1.12

Analysis of mouse brain peptides using mass spectrometry-based peptidomics: implications for novel functions ranging from non-classical neuropeptides to microproteins. Mol Biosyst (2010) 1.05

Pazopanib and sunitinib trigger autophagic and non-autophagic death of bladder tumour cells. Br J Cancer (2013) 1.05

(-)-Epigallocatechin-3-gallate induces non-apoptotic cell death in human cancer cells via ROS-mediated lysosomal membrane permeabilization. PLoS One (2012) 0.99

Intralysosomal iron induces lysosomal membrane permeabilization and cathepsin D-mediated cell death in trabecular meshwork cells exposed to oxidative stress. Invest Ophthalmol Vis Sci (2010) 0.97

Vibrio effector protein, VopQ, forms a lysosomal gated channel that disrupts host ion homeostasis and autophagic flux. Proc Natl Acad Sci U S A (2013) 0.96

Soluble E-cadherin: more than a symptom of disease. Front Biosci (Landmark Ed) (2012) 0.95

Squamous cell carcinoma antigen 1 promotes caspase-8-mediated apoptosis in response to endoplasmic reticulum stress while inhibiting necrosis induced by lysosomal injury. Mol Cell Biol (2011) 0.93

Nanopreparations for organelle-specific delivery in cancer. Adv Drug Deliv Rev (2013) 0.90

Impairment of Atg5-dependent autophagic flux promotes paraquat- and MPP⁺-induced apoptosis but not rotenone or 6-hydroxydopamine toxicity. Toxicol Sci (2013) 0.90

Group XV phospholipase A₂, a lysosomal phospholipase A₂. Prog Lipid Res (2010) 0.90

Thymoquinone inhibits autophagy and induces cathepsin-mediated, caspase-independent cell death in glioblastoma cells. PLoS One (2013) 0.90

Nuclear cysteine cathepsin variants in thyroid carcinoma cells. Biol Chem (2010) 0.88

Concanavalin A/IFN-gamma triggers autophagy-related necrotic hepatocyte death through IRGM1-mediated lysosomal membrane disruption. PLoS One (2011) 0.88

Sphingosine mediates TNFα-induced lysosomal membrane permeabilization and ensuing programmed cell death in hepatoma cells. J Lipid Res (2012) 0.87

Differential Impact of Cysteine Cathepsins on Genetic Mouse Models of De novo Carcinogenesis: Cathepsin B as Emerging Therapeutic Target. Front Pharmacol (2012) 0.86

Tamoxifen toxicity in cultured retinal pigment epithelial cells is mediated by concurrent regulated cell death mechanisms. Invest Ophthalmol Vis Sci (2014) 0.86

Pneumolysin activates macrophage lysosomal membrane permeabilization and executes apoptosis by distinct mechanisms without membrane pore formation. MBio (2014) 0.86

A role for serglycin proteoglycan in mast cell apoptosis induced by a secretory granule-mediated pathway. J Biol Chem (2010) 0.86

Podocytes degrade endocytosed albumin primarily in lysosomes. PLoS One (2014) 0.85

Siramesine triggers cell death through destabilisation of mitochondria, but not lysosomes. Cell Death Dis (2013) 0.85

Caspase-3-independent apoptotic pathways contribute to interleukin-32γ-mediated control of Mycobacterium tuberculosis infection in THP-1 cells. BMC Microbiol (2015) 0.85

The endolysosomal system in cell death and survival. Cold Spring Harb Perspect Biol (2013) 0.85

Increased apoptosis in cancer cells in vitro and in vivo by ceramides in transferrin-modified liposomes. Cancer Biol Ther (2012) 0.83

Haptoglobin genotype-dependent differences in macrophage lysosomal oxidative injury. J Biol Chem (2014) 0.83

Cathepsin inhibition-induced lysosomal dysfunction enhances pancreatic beta-cell apoptosis in high glucose. PLoS One (2015) 0.83

SerpinB1 is critical for neutrophil survival through cell-autonomous inhibition of cathepsin G. Blood (2013) 0.82

Cysteine Cathepsins Activate ELR Chemokines and Inactivate Non-ELR Chemokines. J Biol Chem (2015) 0.82

How to train glioma cells to die: molecular challenges in cell death. J Neurooncol (2015) 0.80

Identification of ageing-associated naturally occurring peptides in human urine. Oncotarget (2015) 0.80

The major isoforms of Bim contribute to distinct biological activities that govern the processes of autophagy and apoptosis in interleukin-7 dependent lymphocytes. Biochim Biophys Acta (2012) 0.80

Low-density lipoprotein receptor-related protein 1 mediates α1-antitrypsin internalization in CD4+ T lymphocytes. J Leukoc Biol (2015) 0.79

Apoptosis and necrosis: two different outcomes of cigarette smoke condensate-induced endothelial cell death. Cell Death Dis (2012) 0.79

The granzyme B-Serpinb9 axis controls the fate of lymphocytes after lysosomal stress. Cell Death Differ (2014) 0.78

Differential sialotranscriptomes of unfed and fed Rhipicephalus haemaphysaloides, with particular regard to differentially expressed genes of cysteine proteases. Parasit Vectors (2015) 0.78

MAGUKs, scaffolding proteins at cell junctions, are substrates of different proteases during apoptosis. Cell Death Dis (2011) 0.78

Developmental Coordination of Gamete Differentiation with Programmed Cell Death in Sporulating Yeast. Eukaryot Cell (2015) 0.77

Cell-centric view of apoptosis and apoptotic cell death-inducing antitumoral strategies. Cancers (Basel) (2011) 0.77

Streptococcus oralis Induces Lysosomal Impairment of Macrophages via Bacterial Hydrogen Peroxide. Infect Immun (2016) 0.77

Effect of gadolinium-based nanoparticles on nuclear DNA damage and repair in glioblastoma tumor cells. J Nanobiotechnology (2016) 0.77

Applications of small molecule probes in dissecting mechanisms of bacterial virulence and host responses. Biochemistry (2013) 0.77

Cadmium overkill: autophagy, apoptosis and necrosis signalling in endothelial cells exposed to cadmium. Cell Mol Life Sci (2015) 0.76

Mefloquine, an anti-malaria agent, causes reactive oxygen species-dependent cell death in mast cells via a secretory granule-mediated pathway. Pharmacol Res Perspect (2014) 0.76

Latency of lysosomal proteinases. J Biol Chem (2009) 0.75

Iowa Mutant Apolipoprotein A-I (ApoA-IIowa) Fibrils Target Lysosomes. Sci Rep (2016) 0.75

Nanodiamonds and nanoparticles as tumor cell radiosensitizers-promising results but an obscure mechanism of action. Ann Transl Med (2017) 0.75

Biochemical Characterization and Substrate Specificity of Autophagin-2 from the Parasite Trypanosoma cruzi. J Biol Chem (2015) 0.75

A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells. Sci Rep (2015) 0.75

A new way to detect the danger: Lysosomal cell death induced by a bacterial ribosomal protein. J Nat Sci (2015) 0.75

CLIC5: a novel ETV6 target gene in childhood acute lymphoblastic leukemia. Haematologica (2016) 0.75

Heterologous expression of anti-apoptotic human 14-3-3β/α enhances iron-mediated programmed cell death in yeast. PLoS One (2017) 0.75

Articles cited by this

Autophagy in the pathogenesis of disease. Cell (2008) 34.68

Autophagy fights disease through cellular self-digestion. Nature (2008) 33.83

Cell death: critical control points. Cell (2004) 22.56

The biochemistry of apoptosis. Nature (2000) 18.91

Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat Rev Mol Cell Biol (2007) 15.47

Autophagy: from phenomenology to molecular understanding in less than a decade. Nat Rev Mol Cell Biol (2007) 10.79

Autophagic cell death: the story of a misnomer. Nat Rev Mol Cell Biol (2008) 6.78

Lysosomes: fusion and function. Nat Rev Mol Cell Biol (2007) 5.74

Lysosomes and autophagy in cell death control. Nat Rev Cancer (2005) 5.47

Cell death by necrosis: towards a molecular definition. Trends Biochem Sci (2006) 4.67

Four deaths and a funeral: from caspases to alternative mechanisms. Nat Rev Mol Cell Biol (2001) 4.61

Growth arrest and autophagy are required for salivary gland cell degradation in Drosophila. Cell (2007) 4.55

Restoration of chaperone-mediated autophagy in aging liver improves cellular maintenance and hepatic function. Nat Med (2008) 4.44

Lysosomal membrane permeabilization in cell death. Oncogene (2008) 4.40

Cathepsin B contributes to TNF-alpha-mediated hepatocyte apoptosis by promoting mitochondrial release of cytochrome c. J Clin Invest (2000) 3.58

At the acidic edge: emerging functions for lysosomal membrane proteins. Trends Cell Biol (2003) 3.26

Lysosomal cysteine proteases: facts and opportunities. EMBO J (2001) 3.06

Perforin-mediated target-cell death and immune homeostasis. Nat Rev Immunol (2006) 3.04

Lysosomes in cell death. Oncogene (2004) 3.00

Heat shock protein 70 promotes cell survival by inhibiting lysosomal membrane permeabilization. J Exp Med (2004) 2.86

Cathepsin B acts as a dominant execution protease in tumor cell apoptosis induced by tumor necrosis factor. J Cell Biol (2001) 2.68

Lysosomal protease pathways to apoptosis. Cleavage of bid, not pro-caspases, is the most likely route. J Biol Chem (2000) 2.62

Participation of autophagy in storage of lysosomes in neurons from mouse models of neuronal ceroid-lipofuscinoses (Batten disease). Am J Pathol (2005) 2.56

Selective disruption of lysosomes in HeLa cells triggers apoptosis mediated by cleavage of Bid by multiple papain-like lysosomal cathepsins. J Biol Chem (2003) 2.30

Emerging roles of cysteine cathepsins in disease and their potential as drug targets. Curr Pharm Des (2007) 2.26

Release of cytochrome c and activation of pro-caspase-9 following lysosomal photodamage involves Bid cleavage. Cell Death Differ (2002) 2.13

Cathepsin D triggers Bax activation, resulting in selective apoptosis-inducing factor (AIF) relocation in T lymphocytes entering the early commitment phase to apoptosis. J Biol Chem (2003) 2.06

Specific aspartyl and calpain proteases are required for neurodegeneration in C. elegans. Nature (2002) 1.97

Cysteine cathepsins trigger caspase-dependent cell death through cleavage of bid and antiapoptotic Bcl-2 homologues. J Biol Chem (2008) 1.85

Cysteine cathepsins: cellular roadmap to different functions. Biochimie (2007) 1.80

Molecular mechanisms and pathophysiology of necrotic cell death. Curr Mol Med (2008) 1.63

ER and oxidative stresses are common mediators of apoptosis in both neurodegenerative and non-neurodegenerative lysosomal storage disorders and are alleviated by chemical chaperones. Hum Mol Genet (2007) 1.60

Lysosomes in iron metabolism, ageing and apoptosis. Histochem Cell Biol (2008) 1.60

Lysosomes, a meeting point of proteins, chaperones, and proteases. J Mol Med (Berl) (1998) 1.59

An intracellular serpin regulates necrosis by inhibiting the induction and sequelae of lysosomal injury. Cell (2007) 1.56

Tumor necrosis factor-related apoptosis-inducing ligand activates a lysosomal pathway of apoptosis that is regulated by Bcl-2 proteins. J Biol Chem (2007) 1.43

Microinjection of cathepsin d induces caspase-dependent apoptosis in fibroblasts. Am J Pathol (2002) 1.43

Apoptotic pathways: involvement of lysosomal proteases. Biol Chem (2002) 1.38

Autophagy is involved in nutritional stress response and differentiation in Trypanosoma cruzi. J Biol Chem (2007) 1.31

Cathepsin-cleaved Bid promotes apoptosis in human neutrophils via oxidative stress-induced lysosomal membrane permeabilization. J Leukoc Biol (2007) 1.28

Protease signalling in cell death: caspases versus cysteine cathepsins. FEBS Lett (2007) 1.26

Regulating cysteine protease activity: essential role of protease inhibitors as guardians and regulators. Curr Pharm Des (2002) 1.23

Lysosomes in apoptosis. Methods Enzymol (2008) 1.13

Cathepsin B mediates TRAIL-induced apoptosis in oral cancer cells. J Cancer Res Clin Oncol (2005) 1.07

Regulation of the activity of lysosomal cysteine proteinases by pH-induced inactivation and/or endogenous protein inhibitors, cystatins. Biol Chem Hoppe Seyler (1995) 1.03

Murine cathepsin F deficiency causes neuronal lipofuscinosis and late-onset neurological disease. Mol Cell Biol (2006) 1.02

Cytosolic acidification and lysosomal alkalinization during TNF-alpha induced apoptosis in U937 cells. Apoptosis (2006) 1.01

Aryl hydrocarbon receptor modulation of tumor necrosis factor-alpha-induced apoptosis and lysosomal disruption in a hepatoma model that is caspase-8-independent. J Biol Chem (2006) 0.94

Necrotic cell death: From reversible mitochondrial uncoupling to irreversible lysosomal permeabilization. Exp Cell Res (2008) 0.92

Stress-induced apoptosis is impaired in cells with a lysosomal targeting defect but is not affected in cells synthesizing a catalytically inactive cathepsin D. Cell Death Differ (2003) 0.90

Apoptosis caused by cathepsins does not require Bid signaling in an in vivo model of progressive myoclonus epilepsy (EPM1). Cell Death Differ (2003) 0.89

Lysosomes and Fas-mediated liver cell death. Biochem J (2007) 0.81

Cysteine cathepsins are not involved in Fas/CD95 signalling in primary skin fibroblasts. FEBS Lett (2007) 0.79

Articles by these authors

Selective disruption of lysosomes in HeLa cells triggers apoptosis mediated by cleavage of Bid by multiple papain-like lysosomal cathepsins. J Biol Chem (2003) 2.30

Emerging roles of cysteine cathepsins in disease and their potential as drug targets. Curr Pharm Des (2007) 2.26

Cysteine cathepsins trigger caspase-dependent cell death through cleavage of bid and antiapoptotic Bcl-2 homologues. J Biol Chem (2008) 1.85

Lysosomes and lysosomal cathepsins in cell death. Biochim Biophys Acta (2011) 1.61

Ferri-liposomes as an MRI-visible drug-delivery system for targeting tumours and their microenvironment. Nat Nanotechnol (2011) 1.41

Apoptotic pathways: involvement of lysosomal proteases. Biol Chem (2002) 1.38

Autophagy is involved in nutritional stress response and differentiation in Trypanosoma cruzi. J Biol Chem (2007) 1.31

Lysosomal cysteine cathepsins: signaling pathways in apoptosis. Biol Chem (2007) 1.29

Protease signalling: the cutting edge. EMBO J (2012) 1.24

Intracellular and extracellular cathepsin B facilitate invasion of MCF-10A neoT cells through reconstituted extracellular matrix in vitro. Exp Cell Res (2003) 1.20

Interaction between human cathepsins K, L, and S and elastins: mechanism of elastinolysis and inhibition by macromolecular inhibitors. J Biol Chem (2007) 1.14

Lysosomes in apoptosis. Methods Enzymol (2008) 1.13

Expression and activity profiling of selected cysteine cathepsins and matrix metalloproteinases in synovial fluids from patients with rheumatoid arthritis and osteoarthritis. Biol Chem (2010) 1.13

Metacaspases of Trypanosoma cruzi: possible candidates for programmed cell death mediators. Mol Biochem Parasitol (2005) 1.10

Exoerythrocytic Plasmodium parasites secrete a cysteine protease inhibitor involved in sporozoite invasion and capable of blocking cell death of host hepatocytes. PLoS Pathog (2010) 1.07

Glycosaminoglycans facilitate procathepsin B activation through disruption of propeptide-mature enzyme interactions. J Biol Chem (2007) 1.04

Intracellular proteolytic activity of cathepsin B is associated with capillary-like tube formation by endothelial cells in vitro. J Cell Biochem (2006) 1.04

Autocatalytic processing of procathepsin B is triggered by proenzyme activity. FEBS J (2009) 1.02

Carboxypeptidases cathepsins X and B display distinct protein profile in human cells and tissues. Exp Cell Res (2005) 1.02

Lysosomal cysteine proteases: structural features and their role in apoptosis. IUBMB Life (2005) 1.01

Recombinant human procathepsin S is capable of autocatalytic processing at neutral pH in the presence of glycosaminoglycans. FEBS Lett (2005) 1.00

Diversity and evolution of the thyroglobulin type-1 domain superfamily. Mol Biol Evol (2005) 0.98

The novel bovine serpin endopin 2C demonstrates selective inhibition of the cysteine protease cathepsin L compared to the serine protease elastase, in cross-class inhibition. Biochemistry (2005) 0.98

Selective activity-based probes for cysteine cathepsins. Angew Chem Int Ed Engl (2008) 0.94

Interaction of human stefin B in the prefibrillar oligomeric form with membranes. Correlation with cellular toxicity. FEBS J (2005) 0.93

Inhibitory properties of cystatin F and its localization in U937 promonocyte cells. FEBS J (2005) 0.93

Phylogenomic analysis of the cystatin superfamily in eukaryotes and prokaryotes. BMC Evol Biol (2009) 0.93

In vitro study of stability and amyloid-fibril formation of two mutants of human stefin B (cystatin B) occurring in patients with EPM1. Protein Sci (2005) 0.91

Human stefin B readily forms amyloid fibrils in vitro. Biochim Biophys Acta (2002) 0.91

Recombinant human cathepsin H lacking the mini chain is an endopeptidase. Biochemistry (2003) 0.89

Mechanisms of amyloid fibril formation--focus on domain-swapping. FEBS J (2011) 0.89

The mechanism of amyloid-fibril formation by stefin B: temperature and protein concentration dependence of the rates. Proteins (2009) 0.87

Recombinant human SMOCs produced by in vitro refolding: calcium-binding properties and interactions with serum proteins. Protein Expr Purif (2008) 0.87

Cleavage of MAGI-1, a tight junction PDZ protein, by caspases is an important step for cell-cell detachment in apoptosis. Apoptosis (2007) 0.86

Exclusion of the native alpha-helix from the amyloid fibrils of a mixed alpha/beta protein. J Mol Biol (2007) 0.86

Interaction with model membranes and pore formation by human stefin B: studying the native and prefibrillar states. FEBS J (2008) 0.86

Human and mouse perforin are processed in part through cleavage by the lysosomal cysteine proteinase cathepsin L. Immunology (2010) 0.85

Interaction between oligomers of stefin B and amyloid-beta in vitro and in cells. J Biol Chem (2009) 0.85

Cellular localization of MAGI-1 caspase cleavage products and their role in apoptosis. Biol Chem (2007) 0.85

Size and morphology of toxic oligomers of amyloidogenic proteins: a case study of human stefin B. Amyloid (2008) 0.85

Blocking autophagy to prevent parasite differentiation: a possible new strategy for fighting parasitic infections? Autophagy (2008) 0.85

Sensitization of stefin B-deficient thymocytes towards staurosporin-induced apoptosis is independent of cysteine cathepsins. FEBS Lett (2005) 0.84

Differential regulation of the intrinsic pathway of apoptosis in brain and liver during ageing. FEBS Lett (2006) 0.84

Differentiation- and maturation-dependent content, localization, and secretion of cystatin C in human dendritic cells. J Leukoc Biol (2005) 0.84

Increased expression of stefin B in the nucleus of T98G astrocytoma cells delays caspase activation. Front Mol Neurosci (2012) 0.84

Structural characterization of thyroglobulin type-1 domains of equistatin. FEBS Lett (2003) 0.84

Intracellular aggregation of human stefin B: confocal and electron microscopy study. Biol Cell (2010) 0.83

Novel aspartyl proteinase associated to fat body histolysis during Ceratitis capitata early metamorphosis. Arch Insect Biochem Physiol (2004) 0.82

Papain-like lysosomal cysteine proteases and their inhibitors: drug discovery targets? Biochem Soc Symp (2003) 0.81

hDLG/SAP97, a member of the MAGUK protein family, is a novel caspase target during cell-cell detachment in apoptosis. Biol Chem (2005) 0.81

Protein aggregation as a possible cause for pathology in a subset of familial Unverricht-Lundborg disease. Med Hypotheses (2005) 0.80

Murine and human cathepsin B exhibit similar properties: possible implications for drug discovery. Biol Chem (2009) 0.80

Inhibitory p41 isoform of invariant chain and its potential target enzymes cathepsins L and H in distinct populations of macrophages in human lymph nodes. Immunology (2004) 0.80

Amyloid fibril formation by human stefin B in vitro: immunogold labelling and comparison to stefin A. Biol Chem (2002) 0.80

Human brain cathepsin H as a neuropeptide and bradykinin metabolizing enzyme. Peptides (2003) 0.79

Developmental shift in the apostat: comparison of neurones and astrocytes. FEBS Lett (2005) 0.79

Cysteine cathepsins are not involved in Fas/CD95 signalling in primary skin fibroblasts. FEBS Lett (2007) 0.79

Mouse stefins A1 and A2 (Stfa1 and Stfa2) differentiate between papain-like endo- and exopeptidases. FEBS Lett (2006) 0.79

The proinflammatory cytokines interleukin-1α and tumor necrosis factor α promote the expression and secretion of proteolytically active cathepsin S from human chondrocytes. Biol Chem (2013) 0.79

Dual concentration-dependent activity of thyroglobulin type-1 domain of testican: specific inhibitor and substrate of cathepsin L. Biol Chem (2005) 0.79

Cysteine cathepsins are not critical for TRAIL- and CD95-induced apoptosis in several human cancer cell lines. Biol Chem (2012) 0.78

N-terminally truncated forms of human cathepsin F accumulate in aggresome-like inclusions. Biochim Biophys Acta (2013) 0.77

Individual recombinant thyroglobulin type-1 domains are substrates for lysosomal cysteine proteinases. Biol Chem (2002) 0.77

Resistance of cathepsin L compared to elastase to proteolysis when complexed with the serpin endopin 2C, and recovery of cathepsin L activity. Biochem Biophys Res Commun (2006) 0.77

The Expression of Lysosomal Proteinases and Their Inhibitors in Breast Cancer: Possible Relationship to Prognosis of the Disease. Pathol Oncol Res (1997) 0.77

Cathepsin C and plasma glutamate carboxypeptidase secreted from Fischer rat thyroid cells liberate thyroxin from the N-terminus of thyroglobulin. Biochimie (2011) 0.77

Modulation of contact order effects in the two-state folding of stefins A and B. Biophys J (2011) 0.77

A novel caspase-7 specific monoclonal antibody. Immunol Lett (2005) 0.77

Human cathepsin F: expression in baculovirus system, characterization and inhibition by protein inhibitors. Biol Chem (2004) 0.76

Conformational changes preceding amyloid-fibril formation of amyloid-beta and stefin B; parallels in pH dependence. Curr Med Chem (2002) 0.76

Do lysosomes induce cell death? IUBMB Life (2006) 0.76

Special issue: Proteolysis 50 years after the discovery of lysosome in honor of Christian de Duve. Biochim Biophys Acta (2012) 0.75

Cysteine cathepsins are not critical for TNF-alpha-induced cell death in T98G and U937 cells. Biochim Biophys Acta (2009) 0.75

Differential regulation of Smac/DIABLO and Hsp-70 during brain maturation. Neuromolecular Med (2007) 0.75

Biochemical characterization and structural modeling of human cathepsin E variant 2 in comparison to the wild-type protein. Biol Chem (2012) 0.75

A structural network associated with the kallikrein-kinin and renin-angiotensin systems. Biol Chem (2010) 0.75

Presence of the propeptide on recombinant lysosomal dipeptidase controls both activation and dimerization. Biol Chem (2007) 0.75

Purification and characterization of two cysteine peptidases of the Mediterranean fruit fly Ceratitis capitata during metamorphosis. Arch Insect Biochem Physiol (2008) 0.75