PubRank

Stephen P Bottomley

Author PubWeight™ 89.98‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 A common fold mediates vertebrate defense and bacterial attack. Science 2007 3.43
2 Functional insights from the distribution and role of homopeptide repeat-containing proteins. Genome Res 2005 1.70
3 The two-stage pathway of ataxin-3 fibrillogenesis involves a polyglutamine-independent step. J Biol Chem 2006 1.66
4 The 1.5 A crystal structure of a prokaryote serpin: controlling conformational change in a heated environment. Structure 2003 1.61
5 Dopamine promotes alpha-synuclein aggregation into SDS-resistant soluble oligomers via a distinct folding pathway. FASEB J 2005 1.51
6 A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotechnol 2006 1.48
7 Molecular gymnastics: serpin structure, folding and misfolding. Curr Opin Struct Biol 2006 1.47
8 Natural HLA class I polymorphism controls the pathway of antigen presentation and susceptibility to viral evasion. J Exp Med 2004 1.43
9 Antigen ligation triggers a conformational change within the constant domain of the alphabeta T cell receptor. Immunity 2009 1.40
10 MyD88 adapter-like (Mal)/TIRAP interaction with TRAF6 is critical for TLR2- and TLR4-mediated NF-kappaB proinflammatory responses. J Biol Chem 2009 1.34
11 Serpins flex their muscle: I. Putting the clamps on proteolysis in diverse biological systems. J Biol Chem 2010 1.32
12 Mechanisms of ataxin-3 misfolding and fibril formation: kinetic analysis of a disease-associated polyglutamine protein. J Mol Biol 2007 1.29
13 The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix. J Biol Chem 2005 1.21
14 The murine orthologue of human antichymotrypsin: a structural paradigm for clade A3 serpins. J Biol Chem 2005 1.20
15 Formation of a high affinity lipid-binding intermediate during the early aggregation phase of alpha-synuclein. Biochemistry 2008 1.18
16 Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation. J Biol Chem 2002 1.17
17 Enhancing the stability and solubility of TEV protease using in silico design. Protein Sci 2007 1.17
18 Structural and functional analysis of the Josephin domain of the polyglutamine protein ataxin-3. Biochem Biophys Res Commun 2004 1.14
19 Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions. J Biol Chem 2010 1.14
20 Multi-domain misfolding: understanding the aggregation pathway of polyglutamine proteins. Protein Eng Des Sel 2009 1.12
21 Small heat-shock proteins interact with a flanking domain to suppress polyglutamine aggregation. Proc Natl Acad Sci U S A 2010 1.11
22 The subtilisin-like protease AprV2 is required for virulence and uses a novel disulphide-tethered exosite to bind substrates. PLoS Pathog 2010 1.10
23 Kinetic instability of the serpin Z alpha1-antitrypsin promotes aggregation. J Mol Biol 2009 1.10
24 Protein expression and refolding--a practical guide to getting the most out of inclusion bodies. Biotechnol Annu Rev 2004 1.08
25 Polyglutamine expansion in ataxin-3 does not affect protein stability: implications for misfolding and disease. J Biol Chem 2004 1.07
26 Acid Denaturation of alpha1-antitrypsin: characterization of a novel mechanism of serpin polymerization. J Mol Biol 2002 1.06
27 Different conformational changes within the F-helix occur during serpin folding, polymerization, and proteinase inhibition. Biochemistry 2004 1.06
28 Antithrombin: in control of coagulation. Int J Biochem Cell Biol 2004 1.03
29 The matrix refolded. Nat Methods 2005 1.01
30 Human embryonic stem cell models of Huntington disease. Reprod Biomed Online 2009 0.99
31 REFOLD: an analytical database of protein refolding methods. Protein Expr Purif 2005 0.99
32 Destabilization of a non-pathological variant of ataxin-3 results in fibrillogenesis via a partially folded intermediate: a model for misfolding in polyglutamine disease. J Mol Biol 2004 0.98
33 Stabilization of neurotoxic soluble beta-sheet-rich conformations of the Alzheimer's disease amyloid-beta peptide. Biophys J 2007 0.98
34 The N terminus of the serpin, tengpin, functions to trap the metastable native state. EMBO Rep 2007 0.97
35 Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering alpha(1)-antitrypsin to inhibit cathepsin proteases. Biochemistry 2002 0.96
36 Purification of polyglutamine proteins. Methods Enzymol 2006 0.94
37 The structure of the bacterial oxidoreductase enzyme DsbA in complex with a peptide reveals a basis for substrate specificity in the catalytic cycle of DsbA enzymes. J Biol Chem 2009 0.94
38 X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation. EMBO J 2006 0.94
39 Probing the role of the F-helix in serpin stability through a single tryptophan substitution. Biochemistry 2002 0.94
40 Physical characterization of serpin conformations. Methods 2004 0.93
41 Flanking domain stability modulates the aggregation kinetics of a polyglutamine disease protein. Protein Sci 2011 0.93
42 A protein family under 'stress' - serpin stability, folding and misfolding. Front Biosci 2005 0.93
43 The structural impact of a polyglutamine tract is location-dependent. Biophys J 2008 0.93
44 Crystal structure of a UDP-glucose-specific glycosyltransferase from a Mycobacterium species. J Biol Chem 2008 0.93
45 DNA accelerates the inhibition of human cathepsin V by serpins. J Biol Chem 2007 0.92
46 Production of recombinant serpins in Escherichia coli. Methods 2004 0.92
47 Serpin polymerization and its role in disease--the molecular basis of alpha1-antitrypsin deficiency. IUBMB Life 2009 0.91
48 PolyQ: a database describing the sequence and domain context of polyglutamine repeats in proteins. Nucleic Acids Res 2010 0.91
49 Expression, purification and characterization of recombinant Z alpha(1)-antitrypsin--the most common cause of alpha(1)-antitrypsin deficiency. Protein Expr Purif 2009 0.91
50 PFD: a database for the investigation of protein folding kinetics and stability. Nucleic Acids Res 2005 0.90
51 Promiscuous beta-strand interactions and the conformational diseases. Curr Med Chem 2004 0.90
52 Structural change in β-sheet A of Z α(1)-antitrypsin is responsible for accelerated polymerization and disease. J Mol Biol 2011 0.90
53 The structure of H-2K(b) and K(bm8) complexed to a herpes simplex virus determinant: evidence for a conformational switch that governs T cell repertoire selection and viral resistance. J Immunol 2004 0.90
54 The REFOLD database: a tool for the optimization of protein expression and refolding. Nucleic Acids Res 2006 0.89
55 The high resolution crystal structure of a native thermostable serpin reveals the complex mechanism underpinning the stressed to relaxed transition. J Biol Chem 2004 0.88
56 A structural basis for loop C-sheet polymerization in serpins. J Mol Biol 2008 0.87
57 In vitro and in silico design of alpha1-antitrypsin mutants with different conformational stabilities. J Mol Biol 2003 0.86
58 Antagonism of antiviral and allogeneic activity of a human public CTL clonotype by a single altered peptide ligand: implications for allograft rejection. J Immunol 2005 0.86
59 How do proteins avoid becoming too stable? Biophysical studies into metastable proteins. Eur Biophys J 2003 0.86
60 Aeropin from the extremophile Pyrobaculum aerophilum bypasses the serpin misfolding trap. J Biol Chem 2007 0.86
61 Conformational properties of the disease-causing Z variant of α1-antitrypsin revealed by theory and experiment. Biophys J 2012 0.85
62 The selective inhibition of serpin aggregation by the molecular chaperone, alpha-crystallin, indicates a nucleation-dependent specificity. J Biol Chem 2003 0.85
63 Structural and biochemical characterization of the oxidoreductase NmDsbA3 from Neisseria meningitidis. J Biol Chem 2008 0.85
64 A biophysical analysis of the tetratricopeptide repeat-rich mitochondrial import receptor, Tom70, reveals an elongated monomer that is inherently flexible, unstable, and unfolds via a multistate pathway. J Biol Chem 2004 0.85
65 Observation and characterization of the interaction between a single immunoglobulin binding domain of protein L and two equivalents of human kappa light chains. J Biol Chem 2003 0.84
66 Preventing serpin aggregation: the molecular mechanism of citrate action upon antitrypsin unfolding. Protein Sci 2008 0.83
67 The role of protein misfolding in the pathogenesis of human diseases. IUBMB Life 2004 0.83
68 The rate of polyQ-mediated aggregation is dramatically affected by the number and location of surrounding domains. J Mol Biol 2011 0.82
69 Recombinant protein quality evaluation: proposal for a minimal information standard. Stand Genomic Sci 2011 0.82
70 Identification of residual structure within denatured antichymotrypsin: implications for serpin folding and misfolding. Biochem Biophys Res Commun 2004 0.82
71 The roles of helix I and strand 5A in the folding, function and misfolding of α1-antitrypsin. PLoS One 2013 0.82
72 Molecular determinants of the mechanism underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharide. J Biol Chem 2002 0.81
73 Nucleocytoplasmic coagulation: an injury-induced aggregation event that disulfide crosslinks proteins and facilitates their removal by plasmin. Cell Rep 2012 0.81
74 Refolding your protein with a little help from REFOLD. Methods Mol Biol 2011 0.81
75 Structural biology: Serpins' mystery solved. Nature 2008 0.80
76 Tracking the unfolding pathway of a multirepeat protein via tryptophan scanning: evidence of localized instability in the mitochondrial import receptor Tom70. J Biol Chem 2006 0.79
77 The loss of tryptophan 194 in antichymotrypsin lowers the kinetic barrier to misfolding. FEBS J 2007 0.79
78 Methods to measure the kinetics of protease inhibition by serpins. Methods Enzymol 2011 0.78
79 Conformational change in the chromatin remodelling protein MENT. PLoS One 2009 0.78
80 Serpins 2005 - fun between the beta-sheets. Meeting report based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia). FEBS J 2005 0.78
81 Determination of the P1', P2' and P3' subsite-specificity of factor Xa. Int J Biochem Cell Biol 2003 0.77
82 Serpins: finely balanced conformational traps. IUBMB Life 2002 0.77
83 Molecular pathways to polyglutamine aggregation. Adv Exp Med Biol 2012 0.77
84 Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stability. Biochemistry 2003 0.77
85 The human serpin proteinase inhibitor-9 self-associates at physiological temperatures. Protein Sci 2004 0.76
86 Identification of a dimeric intermediate in the unfolding pathway for the calcium-binding protein S100B. J Mol Biol 2008 0.76
87 Discovery of amino acid motifs for thrombin cleavage and validation using a model substrate. Biochemistry 2011 0.76
88 Amyloid formation from an α-helix peptide bundle is seeded by 3(10)-helix aggregates. Chemistry 2010 0.76
89 Intracellular production of recombinant serpins in yeast. Methods Enzymol 2011 0.76
90 Conformational behavior and aggregation of ataxin-3 in SDS. PLoS One 2013 0.75
91 A method for the incremental expansion of polyglutamine repeats in recombinant proteins. Methods Mol Biol 2013 0.75
92 Generation and expression of a minimal hybrid Ig-receptor formed between single domains from proteins L and G. Protein Expr Purif 2007 0.75
93 Serpin acceleration of amyloid fibril formation: a role for accessory proteins. J Mol Biol 2006 0.75