Published in Protein Sci on September 02, 2014
Tandem Repeats in Proteins: Prediction Algorithms and Biological Role. Front Bioeng Biotechnol (2015) 0.80
Structure of a 13-fold superhelix (almost) determined from first principles. IUCrJ (2015) 0.75
Structures of designed armadillo-repeat proteins show propagation of inter-repeat interface effects. Acta Crystallogr D Struct Biol (2016) 0.75
Structures of designed armadillo repeat proteins binding to peptides fused to globular domains. Protein Sci (2017) 0.75
Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr (2004) 227.01
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr D Biol Crystallogr (2010) 108.52
Phaser crystallographic software. J Appl Crystallogr (2007) 108.34
XDS. Acta Crystallogr D Biol Crystallogr (2010) 67.46
Scaling and assessment of data quality. Acta Crystallogr D Biol Crystallogr (2005) 61.38
Efficient anisotropic refinement of macromolecular structures using FFT. Acta Crystallogr D Biol Crystallogr (1999) 15.91
Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha. Cell (1998) 5.27
3D domain swapping: a mechanism for oligomer assembly. Protein Sci (1995) 4.96
3D domain swapping: as domains continue to swap. Protein Sci (2002) 4.35
Crystal structure of a stable dimer reveals the molecular basis of serpin polymerization. Nature (2008) 2.88
One-step evolution of a dimer from a monomeric protein. Nat Struct Biol (1995) 1.75
Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins. J Mol Biol (2007) 1.67
Three-dimensional domain swapping in p13suc1 occurs in the unfolded state and is controlled by conserved proline residues. Proc Natl Acad Sci U S A (2001) 1.65
DARPins and other repeat protein scaffolds: advances in engineering and applications. Curr Opin Biotechnol (2011) 1.61
Modeling symmetric macromolecular structures in Rosetta3. PLoS One (2011) 1.52
Engineering an intertwined form of CD2 for stability and assembly. Nat Struct Biol (1998) 1.47
Spontaneous self-assembly of engineered armadillo repeat protein fragments into a folded structure. Structure (2014) 1.41
Out-of-register β-sheets suggest a pathway to toxic amyloid aggregates. Proc Natl Acad Sci U S A (2012) 1.39
Designed armadillo repeat proteins as general peptide-binding scaffolds: consensus design and computational optimization of the hydrophobic core. J Mol Biol (2007) 1.33
Precision is essential for efficient catalysis in an evolved Kemp eliminase. Nature (2013) 1.28
Engineered proteins as specific binding reagents. Curr Opin Biotechnol (2005) 1.24
Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module. J Mol Biol (2010) 1.02
Accessing the global minimum conformation of stefin A dimer by annealing under partially denaturing conditions. J Mol Biol (1999) 1.01
Stabilizing ionic interactions in a full-consensus ankyrin repeat protein. J Mol Biol (2007) 0.99
Characterization and further stabilization of designed ankyrin repeat proteins by combining molecular dynamics simulations and experiments. J Mol Biol (2007) 0.94
Domain swapping proceeds via complete unfolding: a 19F- and 1H-NMR study of the Cyanovirin-N protein. J Am Chem Soc (2012) 0.92
Modular peptide binding: from a comparison of natural binders to designed armadillo repeat proteins. J Struct Biol (2013) 0.90
Residue-resolved stability of full-consensus ankyrin repeat proteins probed by NMR. J Mol Biol (2010) 0.89
Mechanisms of amyloid fibril formation--focus on domain-swapping. FEBS J (2011) 0.89
Optimization of designed armadillo repeat proteins by molecular dynamics simulations and NMR spectroscopy. Protein Sci (2012) 0.84
Structure-based optimization of designed Armadillo-repeat proteins. Protein Sci (2012) 0.83
Disulfide formation and stability of a cysteine-rich repeat protein from Helicobacter pylori. Biochemistry (2006) 0.82
Engineering novel binding proteins from nonimmunoglobulin domains. Nat Biotechnol (2005) 3.67
High-affinity binders selected from designed ankyrin repeat protein libraries. Nat Biotechnol (2004) 3.34
Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. J Mol Biol (2003) 2.81
Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display. Nat Biotechnol (2006) 2.18
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods (2007) 2.04
Designed to be stable: crystal structure of a consensus ankyrin repeat protein. Proc Natl Acad Sci U S A (2003) 1.98
Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism. Structure (2007) 1.93
Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability. J Mol Biol (2005) 1.90
A label-free immunosensor array using single-chain antibody fragments. Proc Natl Acad Sci U S A (2005) 1.85
Biophysical properties of human antibody variable domains. J Mol Biol (2003) 1.80
Allosteric inhibition of aminoglycoside phosphotransferase by a designed ankyrin repeat protein. Structure (2005) 1.75
A designed ankyrin repeat protein evolved to picomolar affinity to Her2. J Mol Biol (2007) 1.71
In-vitro protein evolution by ribosome display and mRNA display. J Immunol Methods (2004) 1.69
Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target. Nat Methods (2007) 1.68
Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins. J Mol Biol (2007) 1.67
Design of multivalent complexes using the barnase*barstar module. Nat Biotechnol (2003) 1.65
Direct identification of ligand-receptor interactions on living cells and tissues. Nat Biotechnol (2012) 1.64
Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity. Proc Natl Acad Sci U S A (2008) 1.63
Evolution of three human GPCRs for higher expression and stability. J Mol Biol (2011) 1.62
DARPins and other repeat protein scaffolds: advances in engineering and applications. Curr Opin Biotechnol (2011) 1.61
Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J Mol Biol (2008) 1.57
Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size. Cancer Res (2010) 1.51
Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity. J Biol Chem (2004) 1.46
Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters. Proc Natl Acad Sci U S A (2013) 1.46
Designing repeat proteins: modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family. J Mol Biol (2003) 1.39
Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering. Methods (2004) 1.37
Structure of the PRYSPRY-domain: implications for autoinflammatory diseases. FEBS Lett (2005) 1.36
Designed armadillo repeat proteins as general peptide-binding scaffolds: consensus design and computational optimization of the hydrophobic core. J Mol Biol (2007) 1.33
Structure of a kinesin-tubulin complex and implications for kinesin motility. Nat Struct Mol Biol (2013) 1.31
Precision is essential for efficient catalysis in an evolved Kemp eliminase. Nature (2013) 1.28
PEGylation and multimerization of the anti-p185HER-2 single chain Fv fragment 4D5: effects on tumor targeting. J Biol Chem (2006) 1.26
Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework. J Biol Chem (2002) 1.25
Engineered proteins as specific binding reagents. Curr Opin Biotechnol (2005) 1.24
Biophysical properties of camelid V(HH) domains compared to those of human V(H)3 domains. Biochemistry (2002) 1.22
Folding of a designed simple ankyrin repeat protein. Protein Sci (2004) 1.20
Stepwise unfolding of ankyrin repeats in a single protein revealed by atomic force microscopy. Biophys J (2005) 1.20
Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: comparison of DARPin and camelid VHH binding mode. J Biol Chem (2009) 1.19
Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins. J Biol Chem (2005) 1.17
A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. Proc Natl Acad Sci U S A (2012) 1.16
Selection and characterization of Her2 binding-designed ankyrin repeat proteins. J Biol Chem (2006) 1.15
A novel strategy to design binding molecules harnessing the modular nature of repeat proteins. FEBS Lett (2003) 1.15
Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli. Proc Natl Acad Sci U S A (2014) 1.15
Protein PEGylation decreases observed target association rates via a dual blocking mechanism. Mol Pharmacol (2005) 1.14
A recombinant immunotoxin derived from a humanized epithelial cell adhesion molecule-specific single-chain antibody fragment has potent and selective antitumor activity. Clin Cancer Res (2003) 1.13
Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations. Proc Natl Acad Sci U S A (2012) 1.09
Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopy. Biophys J (2007) 1.09
DARPins: an efficient targeting domain for lentiviral vectors. Mol Ther (2011) 1.08
Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis. J Mol Biol (2006) 1.08
Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library. Nat Biotechnol (2003) 1.06
Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach. Biochemistry (2003) 1.06
Designed ankyrin repeat proteins (DARPins) from research to therapy. Methods Enzymol (2012) 1.06
Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation. J Mol Biol (2006) 1.06
Two crystal structures of pneumococcal pilus sortase C provide novel insights into catalysis and substrate specificity. J Mol Biol (2009) 1.05
Structure-activity studies in a family of beta-hairpin protein epitope mimetic inhibitors of the p53-HDM2 protein-protein interaction. Chembiochem (2006) 1.04
In vitro selection and characterization of DARPins and Fab fragments for the co-crystallization of membrane proteins: The Na(+)-citrate symporter CitS as an example. J Struct Biol (2007) 1.03
The crystal structure of human pyrin b30.2 domain: implications for mutations associated with familial Mediterranean fever. J Mol Biol (2009) 1.02
Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module. J Mol Biol (2010) 1.02
Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries. Proc Natl Acad Sci U S A (2012) 1.01
Transfer of engineered biophysical properties between different antibody formats and expression systems. Protein Eng Des Sel (2012) 1.00
Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling. J Biol Chem (2011) 1.00
An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS. FEBS Lett (2004) 1.00
Stabilizing ionic interactions in a full-consensus ankyrin repeat protein. J Mol Biol (2007) 0.99
The crystal structure of Helicobacter cysteine-rich protein C at 2.0 A resolution: similar peptide-binding sites in TPR and SEL1-like repeat proteins. J Mol Biol (2004) 0.99
Helicobacter pylori evolution: lineage- specific adaptations in homologs of eukaryotic Sel1-like genes. PLoS Comput Biol (2007) 0.99
EpCAM-targeted delivery of nanocomplexed siRNA to tumor cells with designed ankyrin repeat proteins. Mol Cancer Ther (2009) 0.99
Consensus design of repeat proteins. Chembiochem (2004) 0.98
Structural basis for eliciting a cytotoxic effect in HER2-overexpressing cancer cells via binding to the extracellular domain of HER2. Structure (2013) 0.98
Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation. N Biotechnol (2012) 0.97
DARPins recognizing the tumor-associated antigen EpCAM selected by phage and ribosome display and engineered for multivalency. J Mol Biol (2011) 0.97
Antitumor activity of an epithelial cell adhesion molecule targeted nanovesicular drug delivery system. Mol Cancer Ther (2007) 0.97
Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells. J Mol Biol (2012) 0.97
Stabilizing membrane proteins through protein engineering. Curr Opin Chem Biol (2013) 0.96
The crystal structure of Helicobacter pylori cysteine-rich protein B reveals a novel fold for a penicillin-binding protein. J Biol Chem (2002) 0.96
Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution. J Mol Biol (2012) 0.96
Isolation of intracellular proteinase inhibitors derived from designed ankyrin repeat proteins by genetic screening. J Biol Chem (2006) 0.95
Characterization of the Helicobacter pylori cysteine-rich protein A as a T-helper cell type 1 polarizing agent. Infect Immun (2005) 0.95
Comparison of in vivo selection and rational design of heterodimeric coiled coils. Structure (2002) 0.95
Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability. Proteins (2006) 0.94
Epithelial cell adhesion molecule-targeted drug delivery for cancer therapy. Expert Opin Drug Deliv (2013) 0.94
The secreted Helicobacter cysteine-rich protein A causes adherence of human monocytes and differentiation into a macrophage-like phenotype. FEBS Lett (2009) 0.94
Characterization and further stabilization of designed ankyrin repeat proteins by combining molecular dynamics simulations and experiments. J Mol Biol (2007) 0.94
Polymorphisms at the innate immune receptor TLR2 are associated with Borrelia infection in a wild rodent population. Proc Biol Sci (2013) 0.94
Knowledge-based design of a biosensor to quantify localized ERK activation in living cells. Chem Biol (2013) 0.93
Direct selection of antibodies from complex libraries with the protein fragment complementation assay. J Mol Biol (2006) 0.93
Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution. Protein Eng Des Sel (2010) 0.93
Designed ankyrin repeat proteins (DARPins) as novel isoform-specific intracellular inhibitors of c-Jun N-terminal kinases. ACS Chem Biol (2012) 0.93
The influence of the framework core residues on the biophysical properties of immunoglobulin heavy chain variable domains. Protein Eng Des Sel (2009) 0.93
Structural and pharmacological characterization of novel potent and selective monoclonal antibody antagonists of glucose-dependent insulinotropic polypeptide receptor. J Biol Chem (2013) 0.92
Ribosome display: a technology for selecting and evolving proteins from large libraries. Methods Mol Biol (2011) 0.92
Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen. J Mol Biol (2002) 0.92
Selection and characterization of DARPins specific for the neurotensin receptor 1. Protein Eng Des Sel (2009) 0.92
Stabilization and humanization of a single-chain Fv antibody fragment specific for human lymphocyte antigen CD19 by designed point mutations and CDR-grafting onto a human framework. Protein Eng Des Sel (2009) 0.91
Construction and characterization of protein libraries composed of secondary structure modules. Protein Sci (2002) 0.91
Selection based on the folding properties of proteins with ribosome display. FEBS Lett (2003) 0.91
Surface properties of Helicobacter pylori urease complex are essential for persistence. PLoS One (2010) 0.90
Comparison of the crystal structures of the human manganese superoxide dismutase and the homologous Aspergillus fumigatus allergen at 2-A resolution. J Immunol (2002) 0.90