Published in Anal Chem on February 22, 2012
Top Down proteomics: facts and perspectives. Biochem Biophys Res Commun (2014) 1.14
Fast top-down intact protein characterization with capillary zone electrophoresis-electrospray ionization tandem mass spectrometry. Anal Chem (2013) 1.06
Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for top-down characterization of the Mycobacterium marinum secretome. Anal Chem (2014) 1.01
Optimizing capillary electrophoresis for top-down proteomics of 30-80 kDa proteins. Proteomics (2014) 0.98
Spatially-directed protein identification from tissue sections by top-down LC-MS/MS with electron transfer dissociation. Anal Chem (2013) 0.93
Extensive post-translational modification of active and inactivated forms of endogenous p53. Mol Cell Proteomics (2013) 0.86
Developing top down proteomics to maximize proteome and sequence coverage from cells and tissues. Curr Opin Chem Biol (2013) 0.85
Analysis of protein isoforms: can we do it better? Proteomics (2012) 0.80
Impact of the adenoviral E4 Orf3 protein on the activity and posttranslational modification of p53. J Virol (2015) 0.76
Top-down protein identification of proteasome proteins with nanoLC-FT-ICR-MS employing data-independent fragmentation methods. Proteomics (2014) 0.75
Protected amine labels: a versatile molecular scaffold for multiplexed nominal mass and sub-Da isotopologue quantitative proteomic reagents. J Am Soc Mass Spectrom (2014) 0.75
Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat Biotechnol (2001) 28.01
Universal sample preparation method for proteome analysis. Nat Methods (2009) 16.88
Electrospray ionization for mass spectrometry of large biomolecules. Science (1989) 16.50
Direct analysis of protein complexes using mass spectrometry. Nat Biotechnol (1999) 12.42
Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal (2010) 8.61
Automated reduction and interpretation of high resolution electrospray mass spectra of large molecules. J Am Soc Mass Spectrom (2000) 5.44
Mapping intact protein isoforms in discovery mode using top-down proteomics. Nature (2011) 4.84
Fourier transform ion cyclotron resonance mass spectrometry: a primer. Mass Spectrom Rev (1998) 4.66
Novel linear quadrupole ion trap/FT mass spectrometer: performance characterization and use in the comparative analysis of histone H3 post-translational modifications. J Proteome Res (2004) 4.32
Performance evaluation of a hybrid linear ion trap/orbitrap mass spectrometer. Anal Chem (2006) 4.10
Extending top-down mass spectrometry to proteins with masses greater than 200 kilodaltons. Science (2006) 3.93
Infrared multiphoton dissociation of large multiply charged ions for biomolecule sequencing. Anal Chem (1994) 3.88
Protein identification using sequential ion/ion reactions and tandem mass spectrometry. Proc Natl Acad Sci U S A (2005) 3.77
Top-down proteomics. Anal Chem (2004) 3.01
Top-down mass spectrometry of a 29-kDa protein for characterization of any posttranslational modification to within one residue. Proc Natl Acad Sci U S A (2002) 2.62
Decoding protein modifications using top-down mass spectrometry. Nat Methods (2007) 2.62
The role of electron capture dissociation in biomolecular analysis. Mass Spectrom Rev (2005) 2.42
ProSight PTM 2.0: improved protein identification and characterization for top down mass spectrometry. Nucleic Acids Res (2007) 2.35
High-performance mass spectrometry: Fourier transform ion cyclotron resonance at 14.5 Tesla. Anal Chem (2008) 2.23
Multiplexed size separation of intact proteins in solution phase for mass spectrometry. Anal Chem (2009) 2.15
Activated ion electron capture dissociation for mass spectral sequencing of larger (42 kDa) proteins. Anal Chem (2000) 2.12
'Top down' protein characterization via tandem mass spectrometry. J Mass Spectrom (2002) 2.11
Top-down high-resolution mass spectrometry of cardiac myosin binding protein C revealed that truncation alters protein phosphorylation state. Proc Natl Acad Sci U S A (2009) 2.10
ProSight PTM: an integrated environment for protein identification and characterization by top-down mass spectrometry. Nucleic Acids Res (2004) 2.03
Stepwise evolution of protein native structure with electrospray into the gas phase, 10(-12) to 10(2) s. Proc Natl Acad Sci U S A (2008) 1.91
Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry. Nat Protoc (2008) 1.87
Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR. Proc Natl Acad Sci U S A (2002) 1.72
Gel-eluted liquid fraction entrapment electrophoresis: an electrophoretic method for broad molecular weight range proteome separation. Anal Chem (2008) 1.71
A robust two-dimensional separation for top-down tandem mass spectrometry of the low-mass proteome. J Am Soc Mass Spectrom (2009) 1.65
Top-down proteomics on a chromatographic time scale using linear ion trap fourier transform hybrid mass spectrometers. Anal Chem (2007) 1.60
Top down mass spectrometry of < 60-kDa proteins from Methanosarcina acetivorans using quadrupole FRMS with automated octopole collisionally activated dissociation. Mol Cell Proteomics (2006) 1.57
Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics. FEBS J (2007) 1.47
"Proteotyping": population proteomics of human leukocytes using top down mass spectrometry. Anal Chem (2008) 1.46
Top-down protein sequencing and MS3 on a hybrid linear quadrupole ion trap-orbitrap mass spectrometer. Mol Cell Proteomics (2006) 1.43
In vivo phosphorylation site mapping in mouse cardiac troponin I by high resolution top-down electron capture dissociation mass spectrometry: Ser22/23 are the only sites basally phosphorylated. Biochemistry (2009) 1.41
Size-sorting combined with improved nanocapillary liquid chromatography-mass spectrometry for identification of intact proteins up to 80 kDa. Anal Chem (2010) 1.40
New and automated MSn approaches for top-down identification of modified proteins. J Am Soc Mass Spectrom (2005) 1.33
On the scalability and requirements of whole protein mass spectrometry. Anal Chem (2011) 1.31
Initial experimental characterization of a new ultra-high resolution FTICR cell with dynamic harmonization. J Am Soc Mass Spectrom (2011) 1.27
Robust analysis of the yeast proteome under 50 kDa by molecular-mass-based fractionation and top-down mass spectrometry. Anal Chem (2011) 1.23
Improved ion extraction from a linear octopole ion trap: SIMION analysis and experimental demonstration. J Am Soc Mass Spectrom (2002) 1.22
Fourier-transform mass spectrometry for automated fragmentation and identification of 5-20 kDa proteins in mixtures. Electrophoresis (2002) 1.18
Increased coverage in the transmembrane domain with activated-ion electron capture dissociation for top-down Fourier-transform mass spectrometry of integral membrane proteins. J Proteome Res (2007) 1.17
Intact mass detection, interpretation, and visualization to automate Top-Down proteomics on a large scale. Proteomics (2010) 1.16
Analysis of intact protein isoforms by mass spectrometry. J Biol Chem (2011) 1.15
Peptide and protein analysis by electrospray ionization-mass spectrometry and capillary electrophoresis-mass spectrometry. Anal Biochem (1989) 1.10
Versatile online-offline engine for automated acquisition of high-resolution tandem mass spectra. Anal Chem (2008) 1.09
Sensitive and specific identification of wild type and variant proteins from 8 to 669 kDa using top-down mass spectrometry. Mol Cell Proteomics (2008) 1.09
FT-ICR MS optimization for the analysis of intact proteins. Int J Mass Spectrom (2009) 1.02
Improved molecular weight-based processing of intact proteins for interrogation by quadrupole-enhanced FT MS/MS. J Proteome Res (2004) 1.01
Rapid and effective focusing in a carrier ampholyte solution isoelectric focusing system: a proteome prefractionation tool. J Proteome Res (2008) 0.99
Top-down proteomics reveals novel protein forms expressed in Methanosarcina acetivorans. J Am Soc Mass Spectrom (2009) 0.98
Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Annu Rev Phys Chem (1999) 0.97
Consecutive ion activation for top down mass spectrometry: improved protein sequencing by nozzle-skimmer dissociation. Anal Chem (2005) 0.95
Unit mass baseline resolution for an intact 148 kDa therapeutic monoclonal antibody by Fourier transform ion cyclotron resonance mass spectrometry. Anal Chem (2011) 0.95
Structural characterization of intact proteins is enhanced by prevalent fragmentation pathways rarely observed for peptides. J Am Soc Mass Spectrom (2010) 0.94
Phyloproteomic classification of unsequenced organisms by top-down identification of bacterial proteins using capLC-MS/MS on an Orbitrap. Proteomics (2010) 0.93
Charge State Dependent Fragmentation of Gaseous α-Synuclein Cations via Ion Trap and Beam-Type Collisional Activation. Int J Mass Spectrom (2009) 0.91
Top-down proteomics with a quadrupole time-of-flight mass spectrometer and collision-induced dissociation. Rapid Commun Mass Spectrom (2009) 0.78
Mapping intact protein isoforms in discovery mode using top-down proteomics. Nature (2011) 4.84
Precision proteomics: the case for high resolution and high mass accuracy. Proc Natl Acad Sci U S A (2008) 4.00
A monovalent streptavidin with a single femtomolar biotin binding site. Nat Methods (2006) 3.89
Mutations in TMEM216 perturb ciliogenesis and cause Joubert, Meckel and related syndromes. Nat Genet (2010) 3.66
Functional genomic screen for modulators of ciliogenesis and cilium length. Nature (2010) 3.63
Pervasive combinatorial modification of histone H3 in human cells. Nat Methods (2007) 3.54
Proteoform: a single term describing protein complexity. Nat Methods (2013) 3.48
Quantitative analysis of modified proteins and their positional isomers by tandem mass spectrometry: human histone H4. Anal Chem (2006) 3.08
Deconstruction of iterative multidomain polyketide synthase function. Science (2008) 3.02
Probing protein ligand interactions by automated hydrogen/deuterium exchange mass spectrometry. Anal Chem (2006) 2.89
KIT kinase mutants show unique mechanisms of drug resistance to imatinib and sunitinib in gastrointestinal stromal tumor patients. Proc Natl Acad Sci U S A (2009) 2.84
Lacticin 481: in vitro reconstitution of lantibiotic synthetase activity. Science (2004) 2.83
The MMSET histone methyl transferase switches global histone methylation and alters gene expression in t(4;14) multiple myeloma cells. Blood (2010) 2.71
Decoding protein modifications using top-down mass spectrometry. Nat Methods (2007) 2.62
Certain and progressive methylation of histone H4 at lysine 20 during the cell cycle. Mol Cell Biol (2007) 2.43
The role of electron capture dissociation in biomolecular analysis. Mass Spectrom Rev (2005) 2.42
Shotgun annotation of histone modifications: a new approach for streamlined characterization of proteins by top down mass spectrometry. J Am Chem Soc (2004) 2.36
ProSight PTM 2.0: improved protein identification and characterization for top down mass spectrometry. Nucleic Acids Res (2007) 2.35
Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange. Nat Struct Mol Biol (2004) 2.34
Identification of novel interactions in HIV-1 capsid protein assembly by high-resolution mass spectrometry. J Mol Biol (2003) 2.32
Mass spectrometric characterization of human histone H3: a bird's eye view. J Proteome Res (2006) 2.29
Combinatorial modification of human histone H4 quantitated by two-dimensional liquid chromatography coupled with top down mass spectrometry. J Biol Chem (2008) 2.28
High-performance mass spectrometry: Fourier transform ion cyclotron resonance at 14.5 Tesla. Anal Chem (2008) 2.23
Facile detection of acyl and peptidyl intermediates on thiotemplate carrier domains via phosphopantetheinyl elimination reactions during tandem mass spectrometry. Biochemistry (2006) 2.22
Discovery and in vitro biosynthesis of haloduracin, a two-component lantibiotic. Proc Natl Acad Sci U S A (2006) 2.19
Precise characterization of human histones in the H2A gene family by top down mass spectrometry. J Proteome Res (2006) 2.09
Negative regulation of NF-kappaB action by Set9-mediated lysine methylation of the RelA subunit. EMBO J (2009) 2.05
Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase. Proc Natl Acad Sci U S A (2005) 2.03
ProSight PTM: an integrated environment for protein identification and characterization by top-down mass spectrometry. Nucleic Acids Res (2004) 2.03
Analysis of the ganglion cell layer and photoreceptor layer using optical coherence tomography after idiopathic epiretinal membrane surgery. Graefes Arch Clin Exp Ophthalmol (2014) 1.97
Precise and parallel characterization of coding polymorphisms, alternative splicing, and modifications in human proteins by mass spectrometry. Mol Cell Proteomics (2005) 1.86
Web and database software for identification of intact proteins using "top down" mass spectrometry. Anal Chem (2003) 1.85
Tissue-specific expression and post-translational modification of histone H3 variants. J Proteome Res (2008) 1.84
Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis. Proc Natl Acad Sci U S A (2006) 1.80
Lacticin 481 synthetase phosphorylates its substrate during lantibiotic production. J Am Chem Soc (2005) 1.76
Combined electron capture and infrared multiphoton dissociation for multistage MS/MS in a Fourier transform ion cyclotron resonance mass spectrometer. Anal Chem (2003) 1.75
Evolutionarily assembled cis-regulatory module at a human ciliopathy locus. Science (2012) 1.74
Unusual transformations in the biosynthesis of the antibiotic phosphinothricin tripeptide. Nat Chem Biol (2007) 1.72
Complete protein characterization using top-down mass spectrometry and ultraviolet photodissociation. J Am Chem Soc (2013) 1.69
Secretome analysis reveals an Arabidopsis lipase involved in defense against Alternaria brassicicola. Plant Cell (2005) 1.68
Exact masses and chemical formulas of individual Suwannee River fulvic acids from ultrahigh resolution electrospray ionization Fourier transform ion cyclotron resonance mass spectra. Anal Chem (2003) 1.65
A robust two-dimensional separation for top-down tandem mass spectrometry of the low-mass proteome. J Am Soc Mass Spectrom (2009) 1.65
Processing complex mixtures of intact proteins for direct analysis by mass spectrometry. Anal Chem (2002) 1.63
Mass spectrometric interrogation of thioester-bound intermediates in the initial stages of epothilone biosynthesis. Chem Biol (2004) 1.61
Top-down proteomics on a chromatographic time scale using linear ion trap fourier transform hybrid mass spectrometers. Anal Chem (2007) 1.60
Dichlorination of a pyrrolyl-S-carrier protein by FADH2-dependent halogenase PltA during pyoluteorin biosynthesis. Proc Natl Acad Sci U S A (2005) 1.58
Preferential dimethylation of histone H4 lysine 20 by Suv4-20. J Biol Chem (2008) 1.58
Top down mass spectrometry of < 60-kDa proteins from Methanosarcina acetivorans using quadrupole FRMS with automated octopole collisionally activated dissociation. Mol Cell Proteomics (2006) 1.57
Characterization of amino acid side chain losses in electron capture dissociation. J Am Soc Mass Spectrom (2002) 1.56
Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. Proc Natl Acad Sci U S A (2006) 1.56
Designed fabrication of multifunctional magnetic gold nanoshells and their application to magnetic resonance imaging and photothermal therapy. Angew Chem Int Ed Engl (2006) 1.54
Gene-specific characterization of human histone H2B by electron capture dissociation. J Proteome Res (2006) 1.53
Magnetic fluorescent delivery vehicle using uniform mesoporous silica spheres embedded with monodisperse magnetic and semiconductor nanocrystals. J Am Chem Soc (2006) 1.51
Oncogene-induced cellular senescence elicits an anti-Warburg effect. Proteomics (2013) 1.50
Determination of aberrant O-glycosylation in the IgA1 hinge region by electron capture dissociation fourier transform-ion cyclotron resonance mass spectrometry. J Biol Chem (2005) 1.50
Heterologous production of fosfomycin and identification of the minimal biosynthetic gene cluster. Chem Biol (2006) 1.47
Construction of a hybrid quadrupole/Fourier transform ion cyclotron resonance mass spectrometer for versatile MS/MS above 10 kDa. J Am Soc Mass Spectrom (2004) 1.46
"Proteotyping": population proteomics of human leukocytes using top down mass spectrometry. Anal Chem (2008) 1.46
Proteomic identification of annexins, calcium-dependent membrane binding proteins that mediate osmotic stress and abscisic acid signal transduction in Arabidopsis. Plant Cell (2004) 1.44
Photoreceptor layer features in eyes with closed macular holes: optical coherence tomography findings and correlation with visual outcomes. Am J Ophthalmol (2005) 1.44
Fast reversed-phase liquid chromatography to reduce back exchange and increase throughput in H/D exchange monitored by FT-ICR mass spectrometry. J Am Soc Mass Spectrom (2008) 1.43
Cloning, expression, and biochemical characterization of Streptomyces rubellomurinus genes required for biosynthesis of antimalarial compound FR900098. Chem Biol (2008) 1.41
Size-sorting combined with improved nanocapillary liquid chromatography-mass spectrometry for identification of intact proteins up to 80 kDa. Anal Chem (2010) 1.40
Global assessment of combinatorial post-translational modification of core histones in yeast using contemporary mass spectrometry. LYS4 trimethylation correlates with degree of acetylation on the same H3 tail. J Biol Chem (2007) 1.40
Dissecting non-ribosomal and polyketide biosynthetic machineries using electrospray ionization Fourier-Transform mass spectrometry. Nat Prod Rep (2006) 1.39
Analysis of O-glycan heterogeneity in IgA1 myeloma proteins by Fourier transform ion cyclotron resonance mass spectrometry: implications for IgA nephropathy. Anal Bioanal Chem (2007) 1.37
A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis. Proc Natl Acad Sci U S A (2008) 1.36
Large-scale top-down proteomics of the human proteome: membrane proteins, mitochondria, and senescence. Mol Cell Proteomics (2013) 1.33
Tandem mass spectrometry with ultrahigh mass accuracy clarifies peptide identification by database retrieval. J Proteome Res (2009) 1.33
New and automated MSn approaches for top-down identification of modified proteins. J Am Soc Mass Spectrom (2005) 1.33
Whole exome sequencing identifies a splicing mutation in NSUN2 as a cause of a Dubowitz-like syndrome. J Med Genet (2012) 1.32
On the scalability and requirements of whole protein mass spectrometry. Anal Chem (2011) 1.31
Domain-specific regulation of foxP2 CNS expression by lef1. BMC Dev Biol (2008) 1.31