Published in Proc Natl Acad Sci U S A on April 18, 2014
Unravelling the structural and mechanistic basis of CRISPR-Cas systems. Nat Rev Microbiol (2014) 3.48
Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Science (2014) 2.09
Structural biology. Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target. Science (2014) 2.01
CRISPR-Cas immunity in prokaryotes. Nature (2015) 1.75
Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation. Nat Struct Mol Biol (2014) 1.40
Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Nature (2014) 1.33
Target RNA capture and cleavage by the Cmr type III-B CRISPR-Cas effector complex. Genes Dev (2014) 1.18
Mechanism of foreign DNA recognition by a CRISPR RNA-guided surveillance complex from Pseudomonas aeruginosa. Nucleic Acids Res (2015) 1.17
Structural biology. Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning. Science (2015) 1.15
Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression. Nucleic Acids Res (2014) 1.13
Two distinct DNA binding modes guide dual roles of a CRISPR-Cas protein complex. Mol Cell (2015) 1.13
CRISPR interference and priming varies with individual spacer sequences. Nucleic Acids Res (2015) 1.10
Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System. Cell (2015) 1.09
The structural biology of CRISPR-Cas systems. Curr Opin Struct Biol (2015) 1.07
Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli. Nature (2016) 1.05
A Conserved Structural Chassis for Mounting Versatile CRISPR RNA-Guided Immune Responses. Mol Cell (2015) 1.04
Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3. Proc Natl Acad Sci U S A (2014) 1.00
Type I CRISPR-Cas targets endogenous genes and regulates virulence to evade mammalian host immunity. Cell Res (2016) 0.89
Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems. Mol Cell (2016) 0.89
Mechanism of CRISPR-RNA guided recognition of DNA targets in Escherichia coli. Nucleic Acids Res (2015) 0.88
Three CRISPR-Cas immune effector complexes coexist in Pyrococcus furiosus. RNA (2015) 0.86
Bipartite recognition of target RNAs activates DNA cleavage by the Type III-B CRISPR-Cas system. Genes Dev (2016) 0.86
Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3. Nat Struct Mol Biol (2016) 0.85
Evolutionary Ecology of Prokaryotic Immune Mechanisms. Microbiol Mol Biol Rev (2016) 0.82
DNA targeting by the type I-G and type I-A CRISPR-Cas systems of Pyrococcus furiosus. Nucleic Acids Res (2015) 0.82
Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS. BMC Microbiol (2016) 0.82
Conformational Control of Cascade Interference and Priming Activities in CRISPR Immunity. Mol Cell (2016) 0.81
The role of Cas8 in type I CRISPR interference. Biosci Rep (2015) 0.81
Target DNA recognition and cleavage by a reconstituted Type I-G CRISPR-Cas immune effector complex. Extremophiles (2016) 0.81
Structure Principles of CRISPR-Cas Surveillance and Effector Complexes. Annu Rev Biophys (2015) 0.80
Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex. Cell (2017) 0.79
Diversity of CRISPR-Cas immune systems and molecular machines. Genome Biol (2015) 0.78
A decade of discovery: CRISPR functions and applications. Nat Microbiol (2017) 0.77
DNA Targeting by a Minimal CRISPR RNA-Guided Cascade. Mol Cell (2016) 0.77
Interference activity of a minimal Type I CRISPR-Cas system from Shewanella putrefaciens. Nucleic Acids Res (2015) 0.76
The Influence of Copy-Number of Targeted Extrachromosomal Genetic Elements on the Outcome of CRISPR-Cas Defense. Front Mol Biosci (2016) 0.76
Crystal structure of Thermobifida fusca Cse1 reveals target DNA binding site. Protein Sci (2014) 0.76
Commentary: Type I CRISPR-Cas targets endogenous genes and regulates virulence to evade mammalian host immunity. Front Microbiol (2017) 0.75
Box C/D sRNA stem ends act as stabilizing anchors for box C/D di-sRNPs. Nucleic Acids Res (2016) 0.75
DNA binding properties of the small cascade subunit Csa5. PLoS One (2014) 0.75
CRISPR-cas Adaptive Immunity and the Three Rs. Biosci Rep (2017) 0.75
Recruitment of CRISPR-Cas systems by Tn7-like transposons. Proc Natl Acad Sci U S A (2017) 0.75
Priming In A Permissive Type I-C CRISPR-Cas System Reveals Distinct Dynamics Of Spacer Acquisition And Loss. RNA (2017) 0.75
UCSF Chimera--a visualization system for exploratory research and analysis. J Comput Chem (2004) 112.47
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
Protein structure prediction on the Web: a case study using the Phyre server. Nat Protoc (2009) 32.64
CRISPR provides acquired resistance against viruses in prokaryotes. Science (2007) 29.74
EMAN: semiautomated software for high-resolution single-particle reconstructions. J Struct Biol (1999) 27.52
Small CRISPR RNAs guide antiviral defense in prokaryotes. Science (2008) 17.79
RNA-guided genetic silencing systems in bacteria and archaea. Nature (2012) 17.12
Evolution and classification of the CRISPR-Cas systems. Nat Rev Microbiol (2011) 17.11
Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci U S A (2012) 16.48
EMAN2: an extensible image processing suite for electron microscopy. J Struct Biol (2006) 14.70
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature (2010) 14.21
A new generation of the IMAGIC image processing system. J Struct Biol (1996) 12.57
RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex. Cell (2009) 11.12
Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements. J Mol Evol (2005) 11.05
Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology (2009) 10.67
Automated molecular microscopy: the new Leginon system. J Struct Biol (2005) 10.00
Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence. Proc Natl Acad Sci U S A (2011) 6.89
Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Nat Struct Mol Biol (2011) 6.02
Appion: an integrated, database-driven pipeline to facilitate EM image processing. J Struct Biol (2009) 5.71
Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell (2014) 5.26
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science (2014) 5.13
XMIPP: a new generation of an open-source image processing package for electron microscopy. J Struct Biol (2004) 4.95
Structures of the RNA-guided surveillance complex from a bacterial immune system. Nature (2011) 4.92
CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3. Mol Cell (2012) 4.84
SPARX, a new environment for Cryo-EM image processing. J Struct Biol (2006) 4.66
Essential features and rational design of CRISPR RNAs that function with the Cas RAMP module complex to cleave RNAs. Mol Cell (2012) 3.96
Mechanism of foreign DNA selection in a bacterial adaptive immune system. Mol Cell (2012) 2.77
FindEM--a fast, efficient program for automatic selection of particles from electron micrographs. J Struct Biol (2004) 2.74
ACE: automated CTF estimation. Ultramicroscopy (2005) 2.62
Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions. J Struct Biol (2010) 2.48
In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus. EMBO J (2013) 2.16
Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus. Mol Cell (2013) 1.96
Structure of an RNA silencing complex of the CRISPR-Cas immune system. Mol Cell (2013) 1.82
Structure of the CRISPR interference complex CSM reveals key similarities with cascade. Mol Cell (2013) 1.74
In vitro reconstitution of an Escherichia coli RNA-guided immune system reveals unidirectional, ATP-dependent degradation of DNA target. J Biol Chem (2013) 1.62
CRISPR-based technologies: prokaryotic defense weapons repurposed. Trends Genet (2014) 1.60
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
RNA-guided genetic silencing systems in bacteria and archaea. Nature (2012) 17.12
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell (2013) 13.43
CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell (2013) 9.55
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat Biotechnol (2013) 8.40
Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Science (2010) 6.79
A three-dimensional view of the molecular machinery of RNA interference. Nature (2009) 6.31
Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Nat Struct Mol Biol (2011) 6.02
Structural basis for double-stranded RNA processing by Dicer. Science (2006) 5.86
A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity. Science (2010) 5.84
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science (2014) 5.13
RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions. Proc Natl Acad Sci U S A (2011) 4.58
Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation. Mol Cell (2009) 4.57
Complete subunit architecture of the proteasome regulatory particle. Nature (2012) 4.41
The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature (2006) 4.06
Structural roles for human translation factor eIF3 in initiation of protein synthesis. Science (2005) 3.91
Adaptive strategies of the influenza virus polymerase for replication in humans. Proc Natl Acad Sci U S A (2009) 3.89
Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell (2005) 3.86
Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly. Nature (2005) 3.54
Structure, function, and activator-induced conformations of the CRSP coactivator. Science (2002) 3.52
Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense. Structure (2009) 3.47
In vitro reconstitution of the human RISC-loading complex. Proc Natl Acad Sci U S A (2008) 3.43
Microtubule structure at 8 A resolution. Structure (2002) 3.41
Multiplexed RNA structure characterization with selective 2'-hydroxyl acylation analyzed by primer extension sequencing (SHAPE-Seq). Proc Natl Acad Sci U S A (2011) 3.30
The deepest divergences in land plants inferred from phylogenomic evidence. Proc Natl Acad Sci U S A (2006) 3.10
The structural basis for MCM2-7 helicase activation by GINS and Cdc45. Nat Struct Mol Biol (2011) 2.93
The Ndc80 kinetochore complex forms oligomeric arrays along microtubules. Nature (2010) 2.90
An inhibitory activity in human cells restricts the function of an avian-like influenza virus polymerase. Cell Host Microbe (2008) 2.81
Mechanism of substrate selection by a highly specific CRISPR endoribonuclease. RNA (2012) 2.78
Mechanism of foreign DNA selection in a bacterial adaptive immune system. Mol Cell (2012) 2.77
Structural determinants of RNA recognition and cleavage by Dicer. Nat Struct Mol Biol (2007) 2.67
Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner. Nature (2007) 2.64
Autoinhibition of human dicer by its internal helicase domain. J Mol Biol (2008) 2.62
Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore. Curr Biol (2011) 2.62
Molecular mechanisms of RNA interference. Annu Rev Biophys (2013) 2.58
A conformational switch controls hepatitis delta virus ribozyme catalysis. Nature (2004) 2.57
Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3. Proc Natl Acad Sci U S A (2008) 2.50
An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3. Nat Struct Mol Biol (2011) 2.49
Saccharomyces cerevisiae septins: supramolecular organization of heterooligomers and the mechanism of filament assembly. Proc Natl Acad Sci U S A (2008) 2.46
Ribonuclease revisited: structural insights into ribonuclease III family enzymes. Curr Opin Struct Biol (2006) 2.28
In vitro system capable of differentiating fast Ca2+-triggered content mixing from lipid exchange for mechanistic studies of neurotransmitter release. Proc Natl Acad Sci U S A (2011) 2.27
Precise and heritable genome editing in evolutionarily diverse nematodes using TALENs and CRISPR/Cas9 to engineer insertions and deletions. Genetics (2013) 2.22
Coordinated assembly of human translation initiation complexes by the hepatitis C virus internal ribosome entry site RNA. Proc Natl Acad Sci U S A (2004) 2.22
Structural insights into RNA processing by the human RISC-loading complex. Nat Struct Mol Biol (2009) 2.19
Crystal structure of an RNA tertiary domain essential to HCV IRES-mediated translation initiation. Nat Struct Biol (2002) 2.14
Cap-independent translation is required for starvation-induced differentiation in yeast. Science (2007) 2.05
Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion. Elife (2012) 2.04
An essential GTPase promotes assembly of preribosomal RNA processing complexes. Mol Cell (2005) 2.04
Human CRSP interacts with RNA polymerase II CTD and adopts a specific CTD-bound conformation. Genes Dev (2002) 2.02
Structure of human cGAS reveals a conserved family of second-messenger enzymes in innate immunity. Cell Rep (2013) 1.96
RNA processing enables predictable programming of gene expression. Nat Biotechnol (2012) 1.92
Structural visualization of key steps in human transcription initiation. Nature (2013) 1.91
Recognition of RNA polymerase II and transcription bubbles by XPG, CSB, and TFIIH: insights for transcription-coupled repair and Cockayne Syndrome. Mol Cell (2005) 1.89
Structural and mechanistic insights into hepatitis C viral translation initiation. Nat Rev Microbiol (2006) 1.87
Visualizing flexibility at molecular resolution: analysis of heterogeneity in single-particle electron microscopy reconstructions. Annu Rev Biophys Biomol Struct (2007) 1.81
Nucleotide-dependent conformational changes in the DnaA-like core of the origin recognition complex. Nat Struct Mol Biol (2006) 1.80
eIF3j is located in the decoding center of the human 40S ribosomal subunit. Mol Cell (2007) 1.78
Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms. Nat Struct Mol Biol (2007) 1.78
Architecture and flexibility of the yeast Ndc80 kinetochore complex. J Mol Biol (2008) 1.75
The structural basis for regulated assembly and function of the transcriptional activator NtrC. Genes Dev (2006) 1.74
Architecture of the yeast Rrp44 exosome complex suggests routes of RNA recruitment for 3' end processing. Proc Natl Acad Sci U S A (2007) 1.71
Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry. Mol Cell Proteomics (2007) 1.71
ATP ground- and transition states of bacterial enhancer binding AAA+ ATPases support complex formation with their target protein, sigma54. Structure (2007) 1.69
The orthogonal tilt reconstruction method: an approach to generating single-class volumes with no missing cone for ab initio reconstruction of asymmetric particles. J Struct Biol (2005) 1.69
Community-directed delivery of doxycycline for the treatment of onchocerciasis in areas of co-endemicity with loiasis in Cameroon. Parasit Vectors (2009) 1.68
A revised nomenclature for the human and rodent alpha-tubulin gene family. Genomics (2007) 1.68
Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Nat Struct Mol Biol (2010) 1.66
Microtubule dynamics. J Cell Sci (2002) 1.65
Molecular architecture of human polycomb repressive complex 2. Elife (2012) 1.64
Direct link between RACK1 function and localization at the ribosome in vivo. Mol Cell Biol (2008) 1.61
Structural insights into group II intron catalysis and branch-site selection. Science (2002) 1.58
The j-subunit of human translation initiation factor eIF3 is required for the stable binding of eIF3 and its subcomplexes to 40 S ribosomal subunits in vitro. J Biol Chem (2003) 1.57
Phosphatidylinositol-4,5-bisphosphate promotes budding yeast septin filament assembly and organization. J Mol Biol (2010) 1.53
dsRNA with 5' overhangs contributes to endogenous and antiviral RNA silencing pathways in plants. EMBO J (2009) 1.52
Modeling and automation of sequencing-based characterization of RNA structure. Proc Natl Acad Sci U S A (2011) 1.49
Conformational flexibility in the chromatin remodeler RSC observed by electron microscopy and the orthogonal tilt reconstruction method. Proc Natl Acad Sci U S A (2007) 1.49
Crystallization of RNA and RNA-protein complexes. Methods (2004) 1.48
Human TFIID binds to core promoter DNA in a reorganized structural state. Cell (2013) 1.47
Cryo-electron microscopy studies of human TFIID: conformational breathing in the integration of gene regulatory cues. Structure (2006) 1.46
Mutations in TUBB8 and Human Oocyte Meiotic Arrest. N Engl J Med (2016) 1.46
Crystal structure of the HCV IRES central domain reveals strategy for start-codon positioning. Structure (2011) 1.44
Assembly of an active group II intron-maturase complex by protein dimerization. Biochemistry (2004) 1.42
The crystal structure of the signal recognition particle in complex with its receptor. Science (2011) 1.41
Native tandem and ion mobility mass spectrometry highlight structural and modular similarities in clustered-regularly-interspaced shot-palindromic-repeats (CRISPR)-associated protein complexes from Escherichia coli and Pseudomonas aeruginosa. Mol Cell Proteomics (2012) 1.40
Subunit-dependent modulation of septin assembly: budding yeast septin Shs1 promotes ring and gauze formation. J Cell Biol (2011) 1.40
Substrate-specific kinetics of Dicer-catalyzed RNA processing. J Mol Biol (2010) 1.39
Specificity of RNA-RNA helix recognition. Proc Natl Acad Sci U S A (2002) 1.38
Coupled 5' nucleotide recognition and processivity in Xrn1-mediated mRNA decay. Mol Cell (2011) 1.36
Molecular architecture and conformational flexibility of human RNA polymerase II. Structure (2006) 1.36
Structural insights into RNA interference. Curr Opin Struct Biol (2010) 1.34
Septin filament formation is essential in budding yeast. Dev Cell (2011) 1.33
siRNA repositioning for guide strand selection by human Dicer complexes. Mol Cell (2011) 1.31