Published in Nature on January 13, 2010
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature (2011) 17.38
RNA-guided genetic silencing systems in bacteria and archaea. Nature (2012) 17.12
Evolution and classification of the CRISPR-Cas systems. Nat Rev Microbiol (2011) 17.11
RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol (2013) 15.51
Development and applications of CRISPR-Cas9 for genome engineering. Cell (2014) 13.50
CRISPR-based adaptive immune systems. Curr Opin Microbiol (2011) 8.07
CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. Nat Rev Genet (2010) 7.47
Bacteriophage resistance mechanisms. Nat Rev Microbiol (2010) 7.38
Cas9 as a versatile tool for engineering biology. Nat Methods (2013) 6.87
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9. Nature (2014) 5.70
RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions. Proc Natl Acad Sci U S A (2011) 4.58
Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system. Nat Commun (2012) 4.04
Essential features and rational design of CRISPR RNAs that function with the Cas RAMP module complex to cleave RNAs. Mol Cell (2012) 3.96
Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems. Biol Direct (2011) 3.92
Dynamic properties of the Sulfolobus CRISPR/Cas and CRISPR/Cmr systems when challenged with vector-borne viral and plasmid genes and protospacers. Mol Microbiol (2010) 3.77
Interaction of the Cas6 riboendonuclease with CRISPR RNAs: recognition and cleavage. Structure (2011) 3.58
Unravelling the structural and mechanistic basis of CRISPR-Cas systems. Nat Rev Microbiol (2014) 3.48
Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity. Mol Cell (2012) 3.41
Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature (2012) 3.40
CRISPR interference directs strand specific spacer acquisition. PLoS One (2012) 3.10
Transcription, processing and function of CRISPR cassettes in Escherichia coli. Mol Microbiol (2010) 3.05
Mechanism of foreign DNA selection in a bacterial adaptive immune system. Mol Cell (2012) 2.77
A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair. Mol Microbiol (2010) 2.70
Binding and cleavage of CRISPR RNA by Cas6. RNA (2010) 2.67
Self-targeting by CRISPR: gene regulation or autoimmunity? Trends Genet (2010) 2.62
Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature (2014) 2.33
The phage-host arms race: shaping the evolution of microbes. Bioessays (2011) 2.27
Mature clustered, regularly interspaced, short palindromic repeats RNA (crRNA) length is measured by a ruler mechanism anchored at the precursor processing site. Proc Natl Acad Sci U S A (2011) 2.17
Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity. Cell (2015) 2.16
In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus. EMBO J (2013) 2.16
Protospacer recognition motifs: mixed identities and functional diversity. RNA Biol (2013) 2.00
The Escherichia coli CRISPR system protects from λ lysogenization, lysogens, and prophage induction. J Bacteriol (2010) 2.00
Structure of the Cmr2 subunit of the CRISPR-Cas RNA silencing complex. Structure (2012) 1.97
Dealing with the evolutionary downside of CRISPR immunity: bacteria and beneficial plasmids. PLoS Genet (2013) 1.97
The small, slow and specialized CRISPR and anti-CRISPR of Escherichia and Salmonella. PLoS One (2010) 1.84
Structure of an RNA silencing complex of the CRISPR-Cas immune system. Mol Cell (2013) 1.82
An archaeal immune system can detect multiple protospacer adjacent motifs (PAMs) to target invader DNA. J Biol Chem (2012) 1.77
CRISPR-Cas immunity in prokaryotes. Nature (2015) 1.75
Structure of the CRISPR interference complex CSM reveals key similarities with cascade. Mol Cell (2013) 1.74
Type I-E CRISPR-cas systems discriminate target from non-target DNA through base pairing-independent PAM recognition. PLoS Genet (2013) 1.56
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science (2016) 1.56
Strong bias in the bacterial CRISPR elements that confer immunity to phage. Nat Commun (2013) 1.55
Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell (2015) 1.49
CRISPR interference: a structural perspective. Biochem J (2013) 1.48
RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements. Cold Spring Harb Perspect Biol (2012) 1.47
Conditional tolerance of temperate phages via transcription-dependent CRISPR-Cas targeting. Nature (2014) 1.46
CRISPRTarget: bioinformatic prediction and analysis of crRNA targets. RNA Biol (2013) 1.46
CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli. RNA Biol (2013) 1.45
RNA and DNA Targeting by a Reconstituted Thermus thermophiles Type III-A CRISPR-Cas System. PLoS One (2017) 1.39
Staphylococcus epidermidis pan-genome sequence analysis reveals diversity of skin commensal and hospital infection-associated isolates. Genome Biol (2012) 1.38
Characterization of the CRISPR/Cas subtype I-A system of the hyperthermophilic crenarchaeon Thermoproteus tenax. J Bacteriol (2012) 1.35
Generation of an ICF syndrome model by efficient genome editing of human induced pluripotent stem cells using the CRISPR system. Int J Mol Sci (2013) 1.34
Envelope stress is a trigger of CRISPR RNA-mediated DNA silencing in Escherichia coli. Mol Microbiol (2010) 1.33
Programmable removal of bacterial strains by use of genome-targeting CRISPR-Cas systems. MBio (2014) 1.32
RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus. Mol Cell (2014) 1.32
Bacterial genome instability. Microbiol Mol Biol Rev (2014) 1.31
Adapting to new threats: the generation of memory by CRISPR-Cas immune systems. Mol Microbiol (2014) 1.29
Function and regulation of clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR associated (Cas) systems. Viruses (2012) 1.27
DNA motifs determining the efficiency of adaptation into the Escherichia coli CRISPR array. Proc Natl Acad Sci U S A (2013) 1.25
Genetic characterization of antiplasmid immunity through a type III-A CRISPR-Cas system. J Bacteriol (2013) 1.22
Multiscale model of CRISPR-induced coevolutionary dynamics: diversification at the interface of Lamarck and Darwin. Evolution (2012) 1.21
An archaeal CRISPR type III-B system exhibiting distinctive RNA targeting features and mediating dual RNA and DNA interference. Nucleic Acids Res (2014) 1.19
Impact of small repeat sequences on bacterial genome evolution. Genome Biol Evol (2011) 1.18
Viral diversity threshold for adaptive immunity in prokaryotes. MBio (2012) 1.17
CRISPR-mediated phage resistance and the ghost of coevolution past. Proc Biol Sci (2010) 1.14
Microevolution in cyanobacteria: re-sequencing a motile substrain of Synechocystis sp. PCC 6803. DNA Res (2012) 1.14
Molecular mechanisms of CRISPR-mediated microbial immunity. Cell Mol Life Sci (2014) 1.13
Target motifs affecting natural immunity by a constitutive CRISPR-Cas system in Escherichia coli. PLoS One (2012) 1.12
Viral ancestors of antiviral systems. Viruses (2011) 1.11
CRISPR interference and priming varies with individual spacer sequences. Nucleic Acids Res (2015) 1.10
CRISPR loci reveal networks of gene exchange in archaea. Biol Direct (2011) 1.09
Nature and intensity of selection pressure on CRISPR-associated genes. J Bacteriol (2011) 1.08
CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos. Nat Protoc (2014) 1.08
In vivo protein interactions and complex formation in the Pectobacterium atrosepticum subtype I-F CRISPR/Cas System. PLoS One (2012) 1.07
Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9. J Mol Cell Biol (2015) 1.07
Phage-induced expression of CRISPR-associated proteins is revealed by shotgun proteomics in Streptococcus thermophilus. PLoS One (2012) 1.07
The structural biology of CRISPR-Cas systems. Curr Opin Struct Biol (2015) 1.07
The CRISPR/Cas immune system is an operon regulated by LeuO, H-NS, and leucine-responsive regulatory protein in Salmonella enterica serovar Typhi. J Bacteriol (2011) 1.06
Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli. Nature (2016) 1.05
A Conserved Structural Chassis for Mounting Versatile CRISPR RNA-Guided Immune Responses. Mol Cell (2015) 1.04
Intertwined pathways of programmed cell death in immunity. Immunol Rev (2010) 1.04
Crystal structure of the largest subunit of a bacterial RNA-guided immune complex and its role in DNA target binding. J Biol Chem (2012) 1.03
CRISPR-mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus. Nucleic Acids Res (2014) 1.02
Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon. Open Biol (2013) 1.02
Genome engineering of mammalian haploid embryonic stem cells using the Cas9/RNA system. PeerJ (2013) 1.00
Small RNAs for defence and regulation in archaea. Extremophiles (2012) 0.99
In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex. Nucleic Acids Res (2014) 0.98
Targeted bacterial immunity buffers phage diversity. J Virol (2011) 0.98
Unexpectedly broad target recognition of the CRISPR-mediated virus defence system in the archaeon Sulfolobus solfataricus. Nucleic Acids Res (2013) 0.98
Phage encoded H-NS: a potential achilles heel in the bacterial defence system. PLoS One (2011) 0.98
Genetic determinants of PAM-dependent DNA targeting and pre-crRNA processing in Sulfolobus islandicus. RNA Biol (2013) 0.96
Programmable plasmid interference by the CRISPR-Cas system in Thermococcus kodakarensis. RNA Biol (2013) 0.96
Staphylococcus epidermidis Csm1 is a 3'-5' exonuclease. Nucleic Acids Res (2013) 0.96
Nuclear domain 'knock-in' screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing. Nucleic Acids Res (2015) 0.96
CRISPR-Cas: biology, mechanisms and relevance. Philos Trans R Soc Lond B Biol Sci (2016) 0.93
Identification of novel growth phase- and media-dependent small non-coding RNAs in Streptococcus pyogenes M49 using intergenic tiling arrays. BMC Genomics (2012) 0.91
Mobile CRISPR/Cas-mediated bacteriophage resistance in Lactococcus lactis. PLoS One (2012) 0.89
TALEN and CRISPR/Cas Genome Editing Systems: Tools of Discovery. Acta Naturae (2014) 0.89
CRISPR provides acquired resistance against viruses in prokaryotes. Science (2007) 29.74
The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature (1983) 19.44
Small CRISPR RNAs guide antiviral defense in prokaryotes. Science (2008) 17.79
Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus. Lancet (2006) 15.99
Identification of genes that are associated with DNA repeats in prokaryotes. Mol Microbiol (2002) 14.67
Phage response to CRISPR-encoded resistance in Streptococcus thermophilus. J Bacteriol (2007) 13.67
Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J Bacteriol (1982) 13.49
CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA. Science (2008) 11.82
A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes. PLoS Comput Biol (2005) 11.82
A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Biol Direct (2006) 11.31
Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements. J Mol Evol (2005) 11.05
Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology (2009) 10.67
Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin. Microbiology (2005) 10.20
CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies. Microbiology (2005) 9.83
CRISPR--a widespread system that provides acquired resistance against phages in bacteria and archaea. Nat Rev Microbiol (2008) 8.91
Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus. Science (2003) 7.73
The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats. BMC Bioinformatics (2007) 7.34
Identification of 86 candidates for small non-messenger RNAs from the archaeon Archaeoglobus fulgidus. Proc Natl Acad Sci U S A (2002) 7.04
Sorting of protein A to the staphylococcal cell wall. Cell (1992) 6.48
Allelic replacement in Staphylococcus aureus with inducible counter-selection. Plasmid (2005) 6.11
Virus population dynamics and acquired virus resistance in natural microbial communities. Science (2008) 5.54
CRISPR-based adaptive and heritable immunity in prokaryotes. Trends Biochem Sci (2009) 5.54
A putative viral defence mechanism in archaeal cells. Archaea (2006) 4.84
Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot. Nucleic Acids Res (2007) 4.54
Identification of novel non-coding RNAs as potential antisense regulators in the archaeon Sulfolobus solfataricus. Mol Microbiol (2005) 4.52
Prokaryotic silencing (psi)RNAs in Pyrococcus furiosus. RNA (2008) 4.46
CRISPR families of the crenarchaeal genus Sulfolobus: bidirectional transcription and dynamic properties. Mol Microbiol (2009) 3.37
Analysis of CRISPR in Streptococcus mutans suggests frequent occurrence of acquired immunity against infection by M102-like bacteriophages. Microbiology (2009) 2.72
Characterization of a conjugative staphylococcal mupirocin resistance plasmid. Antimicrob Agents Chemother (1995) 2.05
Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism. Biochem Soc Trans (2009) 2.01
Identification and characterization of the origin of conjugative transfer (oriT) and a gene (nes) encoding a single-stranded endonuclease on the staphylococcal plasmid pGO1. J Bacteriol (1996) 1.87
Thermodynamics-structure relationship of single mismatches in RNA/DNA duplexes. Biochemistry (2000) 1.79
Analysis of CRISPR system function in plant pathogen Xanthomonas oryzae. FEMS Microbiol Lett (2009) 1.57
Origins and Mechanisms of miRNAs and siRNAs. Cell (2009) 20.77
Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell (2004) 13.51
CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA. Science (2008) 11.82
CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. Nat Rev Genet (2010) 7.47
A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila. Cell (2004) 5.69
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A (2013) 3.53
Silencing by small RNAs is linked to endosomal trafficking. Nat Cell Biol (2009) 3.25
Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis. Mol Cell (2013) 2.52
A role for ubiquitin in the spliceosome assembly pathway. Nat Struct Mol Biol (2008) 2.12
Molecular requirements for RNA-induced silencing complex assembly in the Drosophila RNA interference pathway. J Biol Chem (2005) 1.62
Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p. RNA (2006) 1.43
An inside job for siRNAs. Mol Cell (2008) 1.29
RNAi: RISC gets loaded. Cell (2005) 1.15
Short interfering RNA strand selection is independent of dsRNA processing polarity during RNAi in Drosophila. Curr Biol (2006) 1.11
Microbiology: slicer for DNA. Nature (2010) 1.10
"siRNAs and miRNAs": a meeting report on RNA silencing. RNA (2004) 1.02
Invasive DNA, chopped and in the CRISPR. Structure (2009) 0.99
ATP modulates siRNA interactions with an endogenous human Dicer complex. RNA (2005) 0.95
Meiosis-induced alterations in transcript architecture and noncoding RNA expression in S. cerevisiae. RNA (2012) 0.86
Methods in Enzymology. The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations. Preface. Methods Enzymol (2014) 0.84
Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis. Proc Natl Acad Sci U S A (2011) 0.83
Proteomics identification of Drosophila small interfering RNA-associated factors. Mol Cell Proteomics (2010) 0.81
R2D2 leads the silencing trigger to mRNA's death star. Cell (2003) 0.78
Thermodynamic and structural characterization of 2'-nitrogen-modified RNA duplexes. Nucleic Acids Res (2004) 0.77
Separation of Drosophila RNA silencing complexes by native gel electrophoresis. Methods Mol Biol (2005) 0.76
A fork in the road for microRNAs. Nat Struct Mol Biol (2007) 0.76
Quit stalling or you'll be silenced. Cell (2013) 0.75
The Making of an siRNA. Mol Cell (2004) 0.75
Type II-C CRISPR-Cas9 Biology, Mechanism and Application. ACS Chem Biol (2017) 0.75