PubRank

A Pingoud

Author PubWeight™ 119.14‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site. Biochemistry 1984 3.03
2 Horizontal gene transfer contributes to the wide distribution and evolution of type II restriction-modification systems. J Mol Evol 1996 1.82
3 The interaction of the EcoRI restriction endonuclease with its substrate. A physico-chemical study employing natural and synthetic oligonucleotides and polynucleotides. Eur J Biochem 1980 1.75
4 Influence of DNA target melting behavior on real-time PCR quantification. Clin Chem 2000 1.66
5 A site-directed mutagenesis study to identify amino acid residues involved in the catalytic function of the restriction endonuclease EcoRV. Biochemistry 1992 1.62
6 Linear diffusion of restriction endonucleases on DNA. J Biol Chem 1985 1.54
7 Ternary complex formation between elongation factor Tu, GTP and aminoacyl-tRNA: an equilibrium study. Eur J Biochem 1977 1.50
8 Pausing of the restriction endonuclease EcoRI during linear diffusion on DNA. Biochemistry 1994 1.46
9 Kinetic characterization of linear diffusion of the restriction endonuclease EcoRV on DNA. Biochemistry 1998 1.39
10 Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases. Nucleic Acids Res 1986 1.34
11 Linear diffusion of the restriction endonuclease EcoRV on DNA is essential for the in vivo function of the enzyme. EMBO J 1996 1.34
12 The stereochemical course of the restriction endonuclease EcoRI-catalyzed reaction. J Biol Chem 1984 1.33
13 Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis. Biochemistry 1991 1.32
14 Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes. Proc Natl Acad Sci U S A 1993 1.31
15 Accuracy of the EcoRI restriction endonuclease: binding and cleavage studies with oligodeoxynucleotide substrates containing degenerate recognition sequences. Biochemistry 1990 1.31
16 The influence of sequences adjacent to the recognition site on the cleavage of oligodeoxynucleotides by the EcoRI endonuclease. Eur J Biochem 1984 1.30
17 Equivalent and non-equivalent binding sites for tRNA on aminoacyl-tRNA synthetases. Eur J Biochem 1975 1.29
18 Mg2+ confers DNA binding specificity to the EcoRV restriction endonuclease. Biochemistry 1992 1.27
19 Inhibition of Eco RI action by polynucleotides. A characterization of the non-specific binding of the enzyme to DNA. Nucleic Acids Res 1980 1.25
20 Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques. Anal Biochem 1991 1.20
21 Evidence for an evolutionary relationship among type-II restriction endonucleases. Gene 1995 1.19
22 Sau3AI, a monomeric type II restriction endonuclease that dimerizes on the DNA and thereby induces DNA loops. J Biol Chem 2001 1.18
23 Cross-linking of bromodeoxyuridine-substituted oligonucleotides to the EcoRI and EcoRV restriction endonucleases. Eur J Biochem 1986 1.16
24 A fast and accurate enzyme-linked immunosorbent assay for the determination of the DNA cleavage activity of restriction endonucleases. Anal Biochem 1993 1.16
25 The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate. EMBO J 1999 1.15
26 A similar active site for non-specific and specific endonucleases. Nat Struct Biol 1999 1.15
27 A comparison of the structural requirements for DNA cleavage by the isoschizomers HaeIII, BspRI and BsuRI. Eur J Biochem 1985 1.14
28 On the catalytic mechanism of EcoRI and EcoRV. A detailed proposal based on biochemical results, structural data and molecular modelling. FEBS Lett 1992 1.14
29 Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis. Nucleic Acids Res 1996 1.13
30 Distinct steps in the specific binding of tRNA to aminoacyl-tRNA synthetase. Temperature-jump studies on the serine-specific system from yeast and the tyrosine-specific system from Escherichia coli. Eur J Biochem 1976 1.11
31 Introduction of asymmetry in the naturally symmetric restriction endonuclease EcoRV to investigate intersubunit communication in the homodimeric protein. Proc Natl Acad Sci U S A 1996 1.10
32 Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI. Nucleic Acids Res 1986 1.10
33 The elongation factor Tu binds aminoacyl-tRNA in the presence of GDP. J Biol Chem 1982 1.09
34 Transient cleavage kinetics of the Eco RI restriction endonuclease measured in a pulsed quench-flow apparatus: enzyme concentration-dependent activity change. Nucleic Acids Res 1981 1.09
35 Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides. Nucleic Acids Res 1988 1.08
36 On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target. Protein Eng 2000 1.06
37 The effect of several nucleic acid binding drugs on the cleavage of d(GGAATTCC) and pBR 322 by the Eco RI restriction endonuclease. Nucleic Acids Res 1981 1.05
38 Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-SceI. Nucleic Acids Res 1996 1.03
39 Probing the indirect readout of the restriction enzyme EcoRV. Mutational analysis of contacts to the DNA backbone. J Biol Chem 1996 1.03
40 DNA binding specificity of the EcoRV restriction endonuclease is increased by Mg2+ binding to a metal ion binding site distinct from the catalytic center of the enzyme. Biochemistry 1995 1.03
41 The inhibition of the GTPase activating protein-Ha-ras interaction by acidic lipids is due to physical association of the C-terminal domain of the GTPase activating protein with micellar structures. EMBO J 1991 1.02
42 Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye. Anal Biochem 1991 1.01
43 Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases. Biochemistry 1984 1.00
44 Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis. Nucleic Acids Res 1994 0.99
45 Does the restriction endonuclease EcoRV employ a two-metal-Ion mechanism for DNA cleavage? Biochemistry 1997 0.99
46 Aminoacyl transfer ribonucleic acid binding site of the bacterial elongation factor Tu. Biochemistry 1980 0.98
47 Polymerase chain reaction detection of a highly polymorphic VNTR segment in intron 1 of the human p53 gene. Clin Chem 1993 0.98
48 Spermidine increases the accuracy of type II restriction endonucleases. Suppression of cleavage at degenerate, non-symmetrical sites. Eur J Biochem 1985 0.97
49 Sequence preferences in cleavage of dsDNA and ssDNA by the extracellular Serratia marcescens endonuclease. Biochemistry 1995 0.95
50 A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. Protein Expr Purif 1994 0.95
51 The recognition of methylated DNA by the GTP-dependent restriction endonuclease McrBC resides in the N-terminal domain of McrB. Biol Chem 1997 0.95
52 Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease. Eur J Biochem 1996 0.94
53 Engineering novel restriction endonucleases: principles and applications. Trends Biotechnol 1996 0.94
54 Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-. Biochemistry 1989 0.94
55 The role of translocation in ribosomal accuracy. Translocation rates for cognate and noncognate aminoacyl- and peptidyl-tRNAs on Escherichia coli ribosomes. J Biol Chem 1987 0.94
56 Does the specific recognition of DNA by the restriction endonuclease EcoRI involve a linear diffusion step? Investigation of the processivity of the EcoRI endonuclease. Nucleic Acids Res 1983 0.93
57 Fluorescence stopped-flow kinetics of the cleavage of synthetic oligodeoxynucleotides by the EcoRI restriction endonuclease. Biochemistry 1989 0.93
58 Biochemical characterization of Anabaena sp. strain PCC 7120 non-specific nuclease NucA and its inhibitor NuiA. Eur J Biochem 1998 0.93
59 Cleavage experiments with deoxythymidine 3',5'-bis-(p-nitrophenyl phosphate) suggest that the homing endonuclease I-PpoI follows the same mechanism of phosphodiester bond hydrolysis as the non-specific Serratia nuclease. FEBS Lett 1999 0.93
60 Semiautomated quantitative detection of loss of heterozygosity in the tumor suppressor gene p53. Biotechniques 1995 0.92
61 Identification of Asp218 and Asp326 as the principal Mg2+ binding ligands of the homing endonuclease PI-SceI. Biochemistry 2000 0.92
62 Characterization of the interaction between the restriction endonuclease McrBC from E. coli and its cofactor GTP. J Mol Biol 1997 0.92
63 Two identical subunits of the EcoRI restriction endonuclease Co-operate in the binding and cleavage of the palindromic substrate. Eur J Biochem 1982 0.92
64 On the advantage of being a dimer, a case study using the dimeric Serratia nuclease and the monomeric nuclease from Anabaena sp. strain PCC 7120. J Biol Chem 1999 0.91
65 Protein engineering of the restriction endonuclease EcoRV: replacement of an amino acid residue in the DNA binding site leads to an altered selectivity towards unmodified and modified substrates. Biochim Biophys Acta 1994 0.91
66 The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding. Nucleic Acids Res 1998 0.91
67 The production and characterization of artificial heterodimers of the restriction endonuclease EcoRV. Biol Chem 1996 0.91
68 Fluoresceinylthiocarbamyl-tRNATyr: a useful derivative of tRNATyr (E.coli) for physicochemical studies. Nucleic Acids Res 1977 0.90
69 Adenylate kinases from thermosensitive Escherichia coli strains. J Mol Biol 1989 0.90
70 Identification of functionally relevant histidine residues in the apoptotic nuclease CAD. Nucleic Acids Res 2001 0.90
71 Protein engineering of the restriction endonuclease EcoRV--structure-guided design of enzyme variants that recognize the base pairs flanking the recognition site. Eur J Biochem 1998 0.90
72 Plasmid DNA cleavage by MunI restriction enzyme: single-turnover and steady-state kinetic analysis. Biochemistry 1999 0.90
73 Crosslinking the EcoRV restriction endonuclease across the DNA-binding site reveals transient intermediates and conformational changes of the enzyme during DNA binding and catalytic turnover. EMBO J 1998 0.89
74 The pharmacokinetics, bioequivalence and bioavailability of different formulations of metoclopramide in man. Arzneimittelforschung 1981 0.89
75 Determination of the DNA bend angle induced by the restriction endonuclease EcoRV in the presence of Mg2+. J Biol Chem 1993 0.89
76 How many EF-Tu molecules participate in aminoacyl-tRNA binding? Biochimie 1992 0.88
77 Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis 2001 0.88
78 Photocross-linking of the homing endonuclease PI-SceI to its recognition sequence. J Biol Chem 1999 0.88
79 The binding of kirromycin to elongation factor Tu. Structural alterations are responsible for the inhibitory action. Eur J Biochem 1978 0.87
80 Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude. J Mol Biol 1998 0.87
81 Engineering of variants of the restriction endonuclease EcoRV that depend in their cleavage activity on the flexibility of sequences flanking the recognition site. Biochemistry 1998 0.86
82 Mechanism of DNA cleavage by the DNA/RNA-non-specific Anabaena sp. PCC 7120 endonuclease NucA and its inhibition by NuiA. J Mol Biol 2000 0.86
83 The antibiotics kirromycin and pulvomycin bind to different sites on the elongation factor Tu from Escherichia coli. Eur J Biochem 1982 0.86
84 Defining the location and function of domains of McrB by deletion mutagenesis. Biol Chem 1999 0.85
85 Mutational analysis of the function of Gln115 in the EcoRI restriction endonuclease, a critical amino acid for recognition of the inner thymidine residue in the sequence -GAATTC- and for coupling specific DNA binding to catalysis. J Mol Biol 1993 0.85
86 A quantitative microtiter plate nuclease assay based on ethidium/DNA fluorescence. Anal Biochem 1996 0.85
87 Intra- vs intersubunit communication in the homodimeric restriction enzyme EcoRV: Thr 37 and Lys 38 involved in indirect readout are only important for the catalytic activity of their own subunit. Biochemistry 1998 0.84
88 Evidence for substrate-assisted catalysis in the DNA cleavage of several restriction endonucleases. Gene 1995 0.83
89 The GTP-binding domain of McrB: more than just a variation on a common theme? J Mol Biol 1999 0.83
90 Comparison between Taq DNA polymerase and its Stoffel fragment for quantitative real-time PCR with hybridization probes. Biotechniques 2001 0.82
91 Binding of the H-ras p21 GTPase activating protein by the activated epidermal growth factor receptor leads to inhibition of the p21 GTPase activity in vitro. Biochemistry 1992 0.82
92 A model for the PI-SceIxDNA complex based on multiple base and phosphate backbone-specific photocross-links. J Mol Biol 2000 0.82
93 The elongation factor Tu from Escherichia coli, aminoacyl-tRNA, and guanosine tetraphosphate form a ternary complex which is bound by programmed ribosomes. J Biol Chem 1983 0.82
94 The mechanism of DNA cleavage by the type II restriction enzyme EcoRV: Asp36 is not directly involved in DNA cleavage but serves to couple indirect readout to catalysis. Biol Chem 1998 0.82
95 Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants. Biochemistry 1989 0.82
96 Identification of a base-specific contact between the restriction endonuclease SsoII and its recognition sequence by photocross-linking. Nucleic Acids Res 2000 0.81
97 Polymorphism of the pentanucleotide repeat d(AAAAT) within intron 1 of the human tumor suppressor gene p53 (17p13.1). Hum Genet 1995 0.81
98 Numerical analysis of binding studies: a direct procedure avoiding the pitfalls of a Scatchard analysis of equilibrium data for unknown binding models. Int J Biomed Comput 1979 0.81
99 Structural and functional analysis of the homing endonuclease PI-sceI by limited proteolytic cleavage and molecular cloning of partial digestion products. Biochemistry 1998 0.80
100 Analysis of the reaction mechanism of the non-specific endonuclease of Serratia marcescens using an artificial minimal substrate. FEBS Lett 1996 0.80
101 Genetic engineering of Escherichia coli to produce a 1:1 complex of the anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA. Gene 2000 0.80
102 The determination of binding parameters from protection experiments. A quantitative assay for ternary complex formation of elongation factor Tu, GTP, and aminoacyl-tRNA. Anal Biochem 1979 0.79
103 Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis. Biotechniques 2000 0.79
104 Genetic engineering, isolation and characterization of a truncated Escherichia coli elongation factor Tu comprising domains 2 and 3. Biochim Biophys Acta 1990 0.79
105 EcoRV-T94V: a mutant restriction endonuclease with an altered substrate specificity towards modified oligodeoxynucleotides. Protein Eng 1996 0.79
106 Probing the function of individual amino acid residues in the DNA binding site of the EcoRI restriction endonuclease by analysing the toxicity of genetically engineered mutants. Gene 1990 0.79
107 Comparison of N-terminal affinity fusion domains: effect on expression level and product heterogeneity of recombinant restriction endonuclease EcoRV. Appl Microbiol Biotechnol 1994 0.78
108 Genetic engineering, production and characterisation of monomeric variants of the dimeric Serratia marcescens endonuclease. FEBS Lett 1998 0.78
109 Polypeptide sequences involved in the cleavage of DNA by the restriction endonuclease EcoRI. J Biol Chem 1986 0.78
110 [Analysis of binding and cleavage of DNA by the gene conversion PI-SCEI endonuclease using site-directed mutagenesis]. Mol Biol (Mosk) 2000 0.78
111 Mutant species of EF-Tu, altered at position 375, exhibit a reduced affinity for aminoacylated transfer-RNAs. FEBS Lett 1985 0.78
112 The elongation factor Tu . guanosine tetraphosphate complex. Eur J Biochem 1981 0.78
113 A 1H NMR study of the Escherichia coli elongation-factor Tu with guanine nucleotides and the antibiotic kirromycin. FEBS Lett 1981 0.78
114 Structure prediction of the EcoRV DNA methyltransferase based on mutant profiling, secondary structure analysis, comparison with known structures of methyltransferases and isolation of catalytically inactive single mutants. Protein Eng 1996 0.77
115 Affinity modification of the restriction endonuclease SsoII by 2'-aldehyde-containing double stranded DNAs. Biochemistry (Mosc) 2005 0.77
116 Synthesis and biochemical characterization of obligatory dimers of the sugar non-specific nuclease from Serratia marcescens using specifically designed bismaleimidoalkanes as SH-specific crosslinking reagents. J Protein Chem 1999 0.76
117 Interaction of myelin basic protein with mononuclear cells: the primary reaction for the MEM and EMT tests. Br J Cancer 1981 0.75
118 Nucleic acid quantification by delta pH measurement. J Biotechnol 2001 0.75
119 Product analysis of in vitro ribosomal protein synthesis for the assessment of kinetic parameters. Anal Biochem 1985 0.75
120 Quantitative polymerase chain reaction with oligodeoxynucleotide ligation assay/enzyme-linked immunosorbent assay detection. Anal Biochem 1993 0.75
121 The identification and analysis of nucleotides bound to the elongation factor Tu from Escherichia coli. Anal Biochem 1981 0.75
122 Vectors for dual expression of target genes in bacterial and mammalian cells. Biotechniques 2000 0.75
123 Immobilization of sugar-non-specific nucleases by utilizing the streptavidin--biotin interaction. J Biotechnol 2001 0.75
124 A dodecapeptide comprising the extended chain-alpha 4 region of the restriction endonuclease EcoRI specifically binds to the EcoRI recognition site. J Biol Chem 1995 0.75
125 Quantitative polymerase chain reaction with enzyme-linked immunosorbent assay detection of selectively digested amplified sample and control DNA. Anal Biochem 1995 0.75
126 [New derivatives of azobenzene for the directed modification of proteins]. Bioorg Khim 2009 0.75
127 Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Suppressor Gene. Methods Mol Med 1999 0.75
128 Application of oligonucleoside methylphosphonates in the studies on phosphodiester hydrolysis by Serratia endonuclease. Nucleosides Nucleotides 1999 0.75