Published in Nature on January 29, 1976
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol (1978) 75.38
Engineering BioBrick vectors from BioBrick parts. J Biol Eng (2008) 5.98
Revised interpretation of the origin of the pSC101 plasmid. J Bacteriol (1977) 5.51
Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria. Microbiol Rev (1983) 5.45
Selected translocation of plasmid genes: frequency and regional specificity of translocation of the Tn3 element. J Bacteriol (1977) 4.08
Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proc Natl Acad Sci U S A (1981) 3.77
Plasmid vehicles for direct cloning of Escherichia coli promoters. J Bacteriol (1979) 2.87
Construction and characterization of a deletion mutant lacking micF, a proposed regulatory gene for OmpF synthesis in Escherichia coli. J Bacteriol (1985) 2.77
Bacterial resistance to the tetracyclines. Microbiol Rev (1978) 2.52
Mutations of temperature sensitivity in R plasmid pSC101. J Bacteriol (1977) 2.38
Identification and characterization of a self-regulated repressor of translocation of the Tn3 element. Proc Natl Acad Sci U S A (1979) 2.37
Replication and incompatibility properties of plasmid pE194 in Bacillus subtilis. J Bacteriol (1982) 2.34
Regulation of the araC gene of Escherichia coli: catabolite repression, autoregulation, and effect on araBAD expression. Proc Natl Acad Sci U S A (1984) 2.10
Tn5 insertion mutations in the mercuric ion resistance genes derived from plasmid R100. J Bacteriol (1983) 1.94
Replication of plasmids in gram-negative bacteria. Microbiol Rev (1989) 1.84
Primary structure of the essential replicon of the plasmid pSC101. Proc Natl Acad Sci U S A (1983) 1.80
Origin and direction of DNA replication of plasmid RSF1030. Proc Natl Acad Sci U S A (1979) 1.76
Development of an extrachromosomal cloning vector system for use in Borrelia burgdorferi. Proc Natl Acad Sci U S A (2000) 1.75
Bidirection replication from a unique origin in a mini-F plasmid. Proc Natl Acad Sci U S A (1977) 1.70
Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I. Proc Natl Acad Sci U S A (2003) 1.67
ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility. J Bacteriol (1979) 1.64
The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101. Nucleic Acids Res (1983) 1.64
Replication of colicin E1 plasmid DNA in vivo requires no plasmid-encoded proteins. J Bacteriol (1978) 1.63
Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12. J Bacteriol (1978) 1.62
Replication control and switch-off function as observed with a mini-F factor plasmid. J Bacteriol (1981) 1.62
Characterization of SmSu plasmids by restriction endonuclease cleavage and compatibility testing. J Bacteriol (1976) 1.59
Multicopy Tn10 tet plasmids confer sensitivity to induction of tet gene expression. J Bacteriol (1983) 1.56
Plasmids containing insertion elements are potential transposons. Proc Natl Acad Sci U S A (1980) 1.54
Function of micF as an antisense RNA in osmoregulatory expression of the ompF gene in Escherichia coli. J Bacteriol (1987) 1.52
Tetracycline resistance transposon Tn1721: recA-dependent gene amplification and expression of tetracycline resistance. J Bacteriol (1981) 1.46
Physical mapping of a DNA sequence common to plasmids of incompatibility group F I. Proc Natl Acad Sci U S A (1977) 1.43
Location of two relaxation nick sites in R6K and single sites in pSC101 and RSF1010 close to origins of vegetative replication: implication for conjugal transfer of plasmid deoxyribonucleic acid. J Bacteriol (1980) 1.40
Transposon-mediated conjugational transmission of nonconjugative plasmids. J Bacteriol (1980) 1.37
The replication initiator protein of plasmid pSC101 is a transcriptional repressor of its own cistron. Proc Natl Acad Sci U S A (1985) 1.35
Cloning of genes involved in membrane lipid synthesis: effects of amplification of phosphatidylglycerophosphate synthase in Escherichia coli. J Bacteriol (1981) 1.31
R-factor cointegrate formation in Salmonella typhimurium bacteriophage type 201 strains. J Bacteriol (1981) 1.31
Transcriptional organization within an Escherichia coli cell division gene cluster: direction of transcription of the cell separation gene envA. J Bacteriol (1984) 1.30
Regulation of DNA replication: "target" determinant of the replication control elements of plasmid R6-5 lies within a control element gene. Proc Natl Acad Sci U S A (1981) 1.29
The incompatibility product of IncFII R plasmid NR1 controls gene expression in the plasmid replication region. J Bacteriol (1982) 1.28
Deletion of lolB, encoding an outer membrane lipoprotein, is lethal for Escherichia coli and causes accumulation of lipoprotein localization intermediates in the periplasm. J Bacteriol (2001) 1.27
Sequence of a class E tetracycline resistance gene from Escherichia coli and comparison of related tetracycline efflux proteins. J Bacteriol (1993) 1.18
Functional analysis of minichromosome replication: bidirectional and unidirectional replication from the Escherichia coli replication origin, oriC. J Bacteriol (1980) 1.17
Penicillin and tetracycline resistance plasmids in Staphylococcus epidermidis. Antimicrob Agents Chemother (1981) 1.16
A plasmid-encoded outer membrane protein, TraT, enhances resistance of Escherichia coli to phagocytosis. Infect Immun (1984) 1.15
Structure of a promotor on plasmid pMB9 derived from plasmid pSC101. Nucleic Acids Res (1980) 1.15
Insertion element IS102 resides in plasmid pSC101. J Bacteriol (1980) 1.14
Effect of dna mutations on the replication of plasmid pSC101 in Escherichia coli K-12. J Bacteriol (1979) 1.12
Expression of the Serratia marcescens lipoproteins gene in Escherichia coli. J Bacteriol (1981) 1.11
Control of replication and segregation of R plasmid Rts1. J Bacteriol (1976) 1.11
Mutants of the mini-F plasmid pML31 thermosensitive in replication. J Bacteriol (1979) 1.09
Isolation and characterization of replication-deficient mutants of ColE1 plasmids. J Bacteriol (1979) 1.09
Isolation and characterization of ColE1-derived plasmid copy-number mutant. Proc Natl Acad Sci U S A (1978) 1.08
Absence of RNase H allows replication of pBR322 in Escherichia coli mutants lacking DNA polymerase I. Proc Natl Acad Sci U S A (1984) 1.07
Incompatibility and surface exclusion properties of H1 and H2 plasmids. J Bacteriol (1977) 1.05
Binding between the par region of plasmids R1 and pSC101 and the outer membrane fraction of the host bacteria. EMBO J (1983) 1.03
RNAII transcribed by IPTG-induced T7 RNA polymerase is non-functional as a replication primer for ColE1-type plasmids in Escherichia coli. Nucleic Acids Res (1995) 1.03
Copy numbers of coexisting plasmids in Escherichia coli K-12. J Bacteriol (1978) 1.02
New approach to tryptophan production by Escherichia coli: genetic manipulation of composite plasmids in vitro. Appl Environ Microbiol (1982) 1.02
New mutations in and around the L2 disordered loop of the RecA protein modulate recombination and/or coprotease activity. J Bacteriol (1992) 0.93
New pSC101-derivative cloning vectors with elevated copy numbers. Plasmid (2008) 0.91
Effects of the pSC101 partition (par) locus on in vivo DNA supercoiling near the plasmid replication origin. Nucleic Acids Res (1995) 0.88
ColE1 copy number mutants. J Bacteriol (1982) 0.84
Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid. Proc Natl Acad Sci U S A (1978) 0.84
Selection and characterization of ColE1 plasmid mutants that exhibit altered stability and replication. J Bacteriol (1981) 0.83
A mutant defective in partitioning of composite plasmid Rms201. J Bacteriol (1981) 0.83
Simple assay for quantitation of Tn5 inversion events in Escherichia coli and use of the assay in determination of plasmid copy number. J Bacteriol (1988) 0.83
Replication initiates at multiple dispersed sites in the ribosomal DNA plasmid of the protozoan parasite Entamoeba histolytica. Mol Cell Biol (1996) 0.82
Low copy expression vectors for use in Yersinia sp. and related organisms. Plasmid (2012) 0.82
New quantitative methods for measuring plasmid loss rates reveal unexpected stability. Plasmid (2013) 0.82
Transcription events in the origin of replication of plasmid pSC101. J Bacteriol (1989) 0.81
Second replicon in ColV2-K94 mediates the stable coexistence of two incompatible plasmids. J Bacteriol (1984) 0.80
Bidirectional replication of the mini-ColE1 plasmid pVH51. J Bacteriol (1979) 0.80
A rapid and sensitive method for plasmid copy number comparisons. Nucleic Acids Res (1989) 0.77
Replication of plasmid DNA in Proteus mirabilis in limiting concentrations of thymine. J Bacteriol (1978) 0.76
Two internal origins of replication in Streptomyces linear plasmid pFRL1. Appl Environ Microbiol (2010) 0.75
YaxAB, a Yersinia enterocolitica pore-forming toxin regulated by RovA. Infect Immun (2013) 0.75
Frequency of replication from alternative origins in the composite R plasmid NR1. J Bacteriol (1978) 0.75
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol (1978) 75.38
Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A (1972) 57.06
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol (1980) 41.18
Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A (1973) 25.61
Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A (1979) 22.59
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet (1979) 17.16
Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol (1974) 15.89
Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Proc Natl Acad Sci U S A (1973) 13.45
Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev (1976) 12.28
Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli. J Mol Biol (1970) 11.86
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol (1973) 11.40
Replication and transcription of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A (1974) 10.51
Multiple molecular species of circular R-factor DNA isolated from Escherichia coli. Nature (1969) 9.02
Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sci U S A (1975) 8.85
Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. Nature (1978) 8.76
Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc Natl Acad Sci U S A (1975) 7.60
Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts. Proc Natl Acad Sci U S A (1983) 6.79
Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature (1979) 6.56
Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli. J Bacteriol (1986) 6.06
Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans. Mol Gen Genet (1982) 5.99
Transposable genetic elements and plasmid evolution. Nature (1976) 5.58
Revised interpretation of the origin of the pSC101 plasmid. J Bacteriol (1977) 5.51
Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc Natl Acad Sci U S A (1974) 5.47
Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. J Bacteriol (1975) 5.35
Nonchromosomal antibiotic resistance in bacteria. V. Isolation and characterization of R factor mutants exhibiting temperature-sensitive repression of fertility. J Bacteriol (1972) 4.85
Molecular and genetic studies of an R factor system consisting of independent transfer and drug resistance plasmids. J Bacteriol (1973) 4.81
Are chlamydial infections the most prevalent venereal disease? JAMA (1975) 4.79
Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique. J Bacteriol (1983) 4.56
Dengue and chikungunya virus infection in man in Thailand, 1962-1964. I. Observations on hospitalized patients with hemorrhagic fever. Am J Trop Med Hyg (1969) 4.41
Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol Gen Genet (1978) 4.33
Observations related to pathogenesis of dengue hemorrhagic fever. IV. Relation of disease severity to antibody response and virus recovered. Yale J Biol Med (1970) 4.30
Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons. Proc Natl Acad Sci U S A (1974) 4.27
Structure and genomic organization of the mouse dihydrofolate reductase gene. Cell (1980) 4.25
3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate. Gene (1980) 4.22
Differential expression of photosynthesis genes in R. capsulata results from segmental differences in stability within the polycistronic rxcA transcript. Cell (1985) 4.15
Selected translocation of plasmid genes: frequency and regional specificity of translocation of the Tn3 element. J Bacteriol (1977) 4.08
Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance. Cell (1980) 4.01
Isolation of catenated forms of R factor DNA from minicells. Nat New Biol (1971) 3.99
Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. J Bacteriol (1975) 3.88
Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proc Natl Acad Sci U S A (1981) 3.77
Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Nature (1975) 3.70
Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids. J Mol Biol (1976) 3.63
Cloning and endonuclease mapping of the hepatitis B viral genome. Nature (1979) 3.62
Letter: Potential biohazards of recombinant DNA molecules. Science (1974) 3.45
Penicillinase-producing Neisseria gonorrhoeae. Lancet (1976) 3.37
A sensitive radioimmunoassay for detecting products translated from cloned DNA fragments. Cell (1978) 3.30
Hemorrhagic fevers of Southeast Asia and South America: a comparative appraisal. Prog Med Virol (1967) 3.22
Growth-rate dependent regulation of mRNA stability in Escherichia coli. Nature (1984) 3.06
Tsg101: a novel tumor susceptibility gene isolated by controlled homozygous functional knockout of allelic loci in mammalian cells. Cell (1996) 2.92
Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids. Cell (1975) 2.80
The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments. Cell (1986) 2.79
Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1. Proc Natl Acad Sci U S A (1970) 2.76
In vivo site-specific genetic recombination promoted by the EcoRI restriction endonuclease. Proc Natl Acad Sci U S A (1977) 2.74
Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces. Mol Gen Genet (1985) 2.71
Coryneform bacterial endocarditis: difficulties in diagnosis and treatment, presentation of three cases, and review of literature. Mayo Clin Proc (1977) 2.63
The usefulness of preoperative laboratory screening. JAMA (1985) 2.55
An intercistronic stem-loop structure functions as an mRNA decay terminator necessary but insufficient for puf mRNA stability. Cell (1988) 2.53
Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways in Streptomyces coelicolor using DNA microarrays. Genes Dev (2001) 2.49
Shock associated with dengue infection. I. Clinical and physiologic manifestations of dengue hemorrhagic fever in Thailand, 1964. J Pediatr (1966) 2.45
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Identification and characterization of a self-regulated repressor of translocation of the Tn3 element. Proc Natl Acad Sci U S A (1979) 2.37
Plasmid replication functions. II. Cloning analysis of the repA replication region of antibiotic resistance plasmid R6-5. Mol Gen Genet (1979) 2.32
Translocation specificity of the Tn3 element: characterization of sites of multiple insertions. Cell (1980) 2.31
Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1. J Bacteriol (1979) 2.29
Complete nucleotide sequence of the Streptomyces lividans plasmid pIJ101 and correlation of the sequence with genetic properties. J Bacteriol (1988) 2.29
The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo. Cell (1991) 2.27
Carotid endarterectomy--an evidence-based review: report of the Therapeutics and Technology Assessment Subcommittee of the American Academy of Neurology. Neurology (2005) 2.26
RNA degradation in Escherichia coli regulated by 3' adenylation and 5' phosphorylation. Nature (1995) 2.26
Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product. Nature (1980) 2.24
Effect of premature termination of translation on mRNA stability depends on the site of ribosome release. Proc Natl Acad Sci U S A (1987) 2.24
Molecular cloning and analysis of genes for production of K5, K7, K12, and K92 capsular polysaccharides in Escherichia coli. J Bacteriol (1986) 2.23
Prior antiplatelet use does not affect hemorrhage growth or outcome after ICH. Neurology (2009) 2.23
Bovine papilloma virus plasmids replicate randomly in mouse fibroblasts throughout S phase of the cell cycle. Cell (1987) 2.22
Genetic recombination through protoplast fusion in Streptomyces. Nature (1977) 2.14
Purification and characterization of colicin D. J Bacteriol (1972) 2.11
Characterization of the structural gene and putative 5'-regulatory sequences for human proopiomelanocortin. Nature (1982) 2.04
Multiple origins of cultivated grapevine (Vitis vinifera L. ssp. sativa) based on chloroplast DNA polymorphisms. Mol Ecol (2006) 2.02
The TSG101 tumor susceptibility gene is located in chromosome 11 band p15 and is mutated in human breast cancer. Cell (1997) 2.02
The Escherichia coli pcnB gene promotes adenylylation of antisense RNAI of ColE1-type plasmids in vivo and degradation of RNAI decay intermediates. Proc Natl Acad Sci U S A (1993) 1.99
Structural and functional analysis of the par region of the pSC 10 1 plasmid. Cell (1984) 1.98
A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces. Nature (1980) 1.95
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Structural and functional analysis of transcriptional control of the Rhodobacter capsulatus puf operon. J Bacteriol (1989) 1.92
Replication timing of DNA sequences associated with human centromeres and telomeres. Mol Cell Biol (1990) 1.89
A TSG101/MDM2 regulatory loop modulates MDM2 degradation and MDM2/p53 feedback control. Proc Natl Acad Sci U S A (2001) 1.88
Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans. Mol Gen Genet (1981) 1.88
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A simple method for quantitative urine culture. N Engl J Med (1967) 1.85
Transcription of complementary strands of phage lambda-DNA in vivo and in vitro. Proc Natl Acad Sci U S A (1967) 1.80
Site-specific RNase E cleavage of oligonucleotides and inhibition by stem-loops. Nature (1995) 1.79
Genetic analysis of the inter-relationship between plasmid replication and incompatibility. Mol Gen Genet (1979) 1.79
Covalently closed circular DNA molecules of low superhelix density as intermediate forms in plasmid replication. Nature (1976) 1.77