Published in J Mol Biol on June 15, 1977
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A (1979) 72.18
Rapid and simple method for purification of nucleic acids. J Clin Microbiol (1990) 44.73
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A (1977) 40.24
Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol (1987) 26.70
Yeast transformation: a model system for the study of recombination. Proc Natl Acad Sci U S A (1981) 26.54
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol (1985) 25.52
Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc Natl Acad Sci U S A (1981) 22.21
Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res (1985) 21.05
Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A (1978) 18.67
Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci U S A (1984) 18.39
Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci U S A (1989) 18.26
Replacement of chromosome segments with altered DNA sequences constructed in vitro. Proc Natl Acad Sci U S A (1979) 17.55
Gene mapping with recombinant inbreds in maize. Genetics (1988) 17.53
Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA. J Virol (1979) 15.69
Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J Bacteriol (1982) 14.27
Repetitive DNA sequences as probes for Mycobacterium tuberculosis. J Clin Microbiol (1988) 14.26
Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci U S A (1988) 13.85
Molecular population genetics of the alcohol dehydrogenase gene region of Drosophila melanogaster. Genetics (1986) 13.66
An adenovirus type 5 early gene function regulates expression of other early viral genes. Proc Natl Acad Sci U S A (1979) 13.21
Polymorphic DNA region adjacent to the 5' end of the human insulin gene. Proc Natl Acad Sci U S A (1981) 12.86
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc Natl Acad Sci U S A (1983) 12.81
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci U S A (1981) 12.44
Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. EMBO J (1993) 11.30
Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc Natl Acad Sci U S A (1982) 11.15
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Mol Cell Biol (1983) 11.06
General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes. J Virol (1984) 10.98
A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J (1984) 10.28
A highly polymorphic locus in human DNA. Proc Natl Acad Sci U S A (1980) 10.27
Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infect Immun (1986) 10.02
Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Res (1979) 9.33
Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences. Proc Natl Acad Sci U S A (1981) 9.27
Novel bacteriophage lambda cloning vector. Proc Natl Acad Sci U S A (1980) 8.97
Nucleotide sequence of the E coli gene coding for dihydrofolate reductase. Nucleic Acids Res (1980) 8.75
RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA. Proc Natl Acad Sci U S A (1983) 8.61
Cellular localization of human immunodeficiency virus infection within the brains of acquired immune deficiency syndrome patients. Proc Natl Acad Sci U S A (1986) 8.45
Shotgun DNA sequencing using cloned DNase I-generated fragments. Nucleic Acids Res (1981) 8.23
Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus. J Bacteriol (1988) 8.18
Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Res (1979) 8.00
The detection of DNA-binding proteins by protein blotting. Nucleic Acids Res (1980) 7.84
Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc Natl Acad Sci U S A (1982) 7.81
Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein. Proc Natl Acad Sci U S A (1986) 7.78
A strategy to reveal high-frequency RFLPs along the human X chromosome. Am J Hum Genet (1984) 7.73
Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. Nucleic Acids Res (1985) 7.67
Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules. Mol Cell Biol (1982) 7.49
A gene activated by growth factors is related to the oncogene v-jun. Proc Natl Acad Sci U S A (1988) 7.47
Interspecies homology of nitrogenase genes. Proc Natl Acad Sci U S A (1980) 7.47
Lc, a member of the maize R gene family responsible for tissue-specific anthocyanin production, encodes a protein similar to transcriptional activators and contains the myc-homology region. Proc Natl Acad Sci U S A (1989) 7.09
Immunological method for mapping genes on Drosophila polytene chromosomes. Proc Natl Acad Sci U S A (1982) 7.08
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence. Mol Cell Biol (1986) 7.07
Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proc Natl Acad Sci U S A (1982) 7.04
Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin. Proc Natl Acad Sci U S A (1989) 7.00
Both Epstein-Barr virus (EBV)-encoded trans-acting factors, EB1 and EB2, are required to activate transcription from an EBV early promoter. EMBO J (1986) 6.82
Sequence, structure, and codon preference of the Drosophila ribosomal protein 49 gene. Nucleic Acids Res (1984) 6.78
Cloning of plasmid DNA sequences involved in invasion of HeLa cells by Shigella flexneri. Infect Immun (1985) 6.74
Bovine papillomavirus contains multiple transforming genes. Proc Natl Acad Sci U S A (1985) 6.73
Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. J Bacteriol (1985) 6.72
Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species. J Bacteriol (1987) 6.71
A member of a new repeated sequence family which is conserved throughout eucaryotic evolution is found between the human delta and beta globin genes. Nucleic Acids Res (1981) 6.70
Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J Virol (1987) 6.65
Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains. J Virol (1996) 6.64
Computer-assisted methods for assessing strain relatedness in Candida albicans by fingerprinting with the moderately repetitive sequence Ca3. J Clin Microbiol (1990) 6.57
Organization, distribution, and stability of endogenous ecotropic murine leukemia virus DNA sequences in chromosomes of Mus musculus. J Virol (1982) 6.56
Transformation of Aspergillus nidulans by using a trpC plasmid. Proc Natl Acad Sci U S A (1984) 6.46
Restriction map variation in the Adh region of Drosophila. Proc Natl Acad Sci U S A (1982) 6.45
A restriction map of the bacteriophage T4 genome. Mol Gen Genet (1980) 6.37
A human beta-actin expression vector system directs high-level accumulation of antisense transcripts. Proc Natl Acad Sci U S A (1987) 6.36
High-level tetracycline resistance in Neisseria gonorrhoeae is result of acquisition of streptococcal tetM determinant. Antimicrob Agents Chemother (1986) 6.34
Deletion of the H19 differentially methylated domain results in loss of imprinted expression of H19 and Igf2. Genes Dev (1998) 6.29
Differences in B cell growth phenotype reflect novel patterns of Epstein-Barr virus latent gene expression in Burkitt's lymphoma cells. EMBO J (1987) 6.26
Heterogeneity of tetracycline resistance determinants in Streptococcus. J Bacteriol (1982) 6.20
Temporal patterns of human cytomegalovirus transcription: mapping the viral RNAs synthesized at immediate early, early, and late times after infection. J Virol (1982) 6.20
Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am J Pathol (1985) 6.18
Structure and variability of human chromosome ends. Mol Cell Biol (1990) 6.18
FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA. J Virol (1982) 6.11
Efficient cloning of genes of Neurospora crassa. Proc Natl Acad Sci U S A (1986) 6.08
Physical map of chromosomal nitrogen fixation (nif) genes of Klebsiella pneumoniae. Proc Natl Acad Sci U S A (1979) 5.93
Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae. Nucleic Acids Res (1982) 5.90
DNA modification of a maize transposable element correlates with loss of activity. Proc Natl Acad Sci U S A (1986) 5.87
Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol Cell Biol (1987) 5.84
Expression of cellular homologues of retroviral onc genes in human hematopoietic cells. Proc Natl Acad Sci U S A (1982) 5.80
A deletion mutation in the 5' part of the pol gene of Moloney murine leukemia virus blocks proteolytic processing of the gag and pol polyproteins. J Virol (1985) 5.78
Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene. Mol Cell Biol (1986) 5.77
Isolation and localization of DNA segments from specific human chromosomes. Proc Natl Acad Sci U S A (1980) 5.76
Organization and expression of the immediate early genes of human cytomegalovirus. J Virol (1983) 5.74
Centromeric DNA from chromosome VI in Saccharomyces cerevisiae strains. EMBO J (1982) 5.71
Molecular cloning and DNA sequence of the Arabidopsis thaliana alcohol dehydrogenase gene. Proc Natl Acad Sci U S A (1986) 5.64
Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate. Nucleic Acids Res (1983) 5.61
Genetic transposition and insertional mutagenesis in Bacillus subtilis with Streptococcus faecalis transposon Tn917. Proc Natl Acad Sci U S A (1983) 5.57
Encapsidation sequences for spleen necrosis virus, an avian retrovirus, are between the 5' long terminal repeat and the start of the gag gene. Proc Natl Acad Sci U S A (1982) 5.56
Hybrid plasmids containing an active thymidine kinase gene of Herpes simplex virus 1. Nucleic Acids Res (1979) 5.55
trans activation of the latent Epstein-Barr virus (EBV) genome after transfection of the EBV DNA fragment. J Virol (1986) 5.25
Termination-reinitiation occurs in the translation of mammalian cell mRNAs. Mol Cell Biol (1986) 5.24
Diagnostic deoxyribonucleic acid probes for infectious diseases. Clin Microbiol Rev (1988) 5.23
Transformation and replication in mouse cells of a bovine papillomavirus--pML2 plasmid vector that can be rescued in bacteria. Proc Natl Acad Sci U S A (1982) 5.22
Complexity of the early genetic response to growth factors in mouse fibroblasts. Mol Cell Biol (1988) 5.21
Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol Cell Biol (1986) 5.16
Extreme instability of myc mRNA in normal and transformed human cells. Proc Natl Acad Sci U S A (1984) 5.09
Integration of the adeno-associated virus genome into cellular DNA in latently infected human Detroit 6 cells. J Virol (1980) 5.09
Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation. J Bacteriol (1984) 5.03
Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet (1982) 54.79
High-efficiency cloning of full-length cDNA. Mol Cell Biol (1982) 22.81
Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc Natl Acad Sci U S A (1981) 22.21
A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol Cell Biol (1983) 18.32
Expression of a bacterial gene in mammalian cells. Science (1980) 15.46
Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic Acids Res (1987) 12.80
Deletion mapping of DNA regions required for SV40 early region promoter function in vivo. J Mol Appl Genet (1982) 10.71
Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature (1979) 9.99
Construction and analysis of viable deletion mutants of simian virus 40. J Virol (1976) 9.11
Cleavage of Simian virus 40 DNA at a unique site by a bacterial restriction enzyme. Proc Natl Acad Sci U S A (1972) 8.96
Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli. Proc Natl Acad Sci U S A (1972) 8.82
Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids. Nature (1981) 8.66
Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40. Proc Natl Acad Sci U S A (1975) 7.57
Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses. Genes Dev (1988) 7.52
Organization and expression of early genes of simian virus 40. Proc Natl Acad Sci U S A (1978) 7.10
Defective simian virus 40 genomes: isolation and growth of individual clones. Virology (1974) 6.30
Negative regulation of viral enhancers in undifferentiated embryonic stem cells. Cell (1985) 5.88
Guidelines for using human event-related potentials to study cognition: recording standards and publication criteria. Psychophysiology (2000) 5.83
McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucleic Acids Res (1988) 5.76
Hybridization in situ of SV40 plaques: detection of recombinant SV40 virus carrying specific sequences of nonviral DNA. Science (1977) 5.75
Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase. Proc Natl Acad Sci U S A (1983) 5.50
Termination-reinitiation occurs in the translation of mammalian cell mRNAs. Mol Cell Biol (1986) 5.24
Immunoglobulin gene expression in transformed lymphoid cells. Proc Natl Acad Sci U S A (1983) 5.18
Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol Cell Biol (1981) 5.00
Comparison of intron-dependent and intron-independent gene expression. Mol Cell Biol (1988) 4.75
A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo. Nature (1990) 4.69
Biochemical procedure for production of small deletions in simian virus 40 DNA. Proc Natl Acad Sci U S A (1975) 4.59
Know your neighbors: three phenotypes in null mutants of the myogenic bHLH gene MRF4. Cell (1996) 4.55
Studies on the binding of RNA polymerase to polynucleotides. J Mol Biol (1966) 4.47
Cleavage of circular, superhelical simian virus 40 DNA to a linear duplex by S1 nuclease. J Virol (1973) 4.39
New region of the simian virus 40 genome required for efficient viral transformation. Proc Natl Acad Sci U S A (1978) 4.24
Physical and genetic characterization of deletion mutants of simian virus 40 constructed in vitro. J Virol (1977) 4.23
Activation of mouse genes in transformed cells. Cell (1983) 3.85
Instability of a missense suppressor resulting from a duplication of genetic material. J Mol Biol (1969) 3.84
Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins. Proc Natl Acad Sci U S A (1995) 3.79
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Viable deletion mutants of simian virus 40: selective isolation by means of a restriction endonuclease from Hemophilus parainfluenzae. Proc Natl Acad Sci U S A (1974) 3.71
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Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis. Genes Dev (1991) 3.46
Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein. J Bacteriol (1984) 3.45
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Factors governing the expression of a bacterial gene in mammalian cells. Mol Cell Biol (1981) 2.74
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Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulum. Nature (1984) 2.66
Homologous recombination between defective neo genes in mouse 3T6 cells. Cold Spring Harb Symp Quant Biol (1984) 2.65
Mutational alterations within the simian virus 40 leader segment generate altered 16S and 19S mRNA's. J Virol (1979) 2.61
Analysis of gene targeting and intrachromosomal homologous recombination stimulated by genomic double-strand breaks in mouse embryonic stem cells. Mol Cell Biol (1998) 2.60
Simian virus 40 early- and late-region promoter functions are enhanced by the 72-base-pair repeat inserted at distant locations and inverted orientations. Mol Cell Biol (1983) 2.53
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Rapid assay for detection of Escherichia coli xanthine-guanine phosphoribosyltransferase activity in transduced cells. Nucleic Acids Res (1985) 2.41
Isolation and characterization of an alphoid centromeric repeat family from the human Y chromosome. J Mol Biol (1985) 2.39
Expression of the type 1 human immunodeficiency virus Nef protein in T cells prevents antigen receptor-mediated induction of interleukin 2 mRNA. Proc Natl Acad Sci U S A (1991) 2.38
Bacteriophage lambda vector for transducing a cDNA clone library into mammalian cells. Mol Cell Biol (1985) 2.35
Renaturation of complementary DNA strands mediated by purified mammalian heterogeneous nuclear ribonucleoprotein A1 protein: implications for a mechanism for rapid molecular assembly. Proc Natl Acad Sci U S A (1990) 2.31
Construction and analysis of viable deletion mutants of polyoma virus. J Virol (1979) 2.30
Homologous recombination of copackaged retrovirus RNAs during reverse transcription. J Virol (1992) 2.29
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Introduction of liposome-encapsulated SV40 DNA into cells. J Biol Chem (1980) 2.23
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Nucleotide sequence deletions within the coding region for small-t antigen of simian virus 40. J Virol (1979) 2.23
Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase. J Bacteriol (1971) 2.23
A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52. Mol Cell Biol (1995) 2.21
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RAD3 gene of Saccharomyces cerevisiae: nucleotide sequence of wild-type and mutant alleles, transcript mapping, and aspects of gene regulation. Mol Cell Biol (1985) 2.16
Gene duplication in experimental enzyme evolution. Nature (1974) 2.16
Activation of a Qa/Tla class I major histocompatibility antigen gene is a general feature of oncogenesis in the mouse. Nature (1984) 2.13
Altering the specificity of restriction endonuclease: effect of replacing Mg2+ with Mn2+. Biochemistry (1978) 2.12
On the properties and utility of a membrane filter assay in the study of isoleucyl-tRNA synthetase. Anal Biochem (1970) 2.08
Isolation and partial characterization of Escherichia coli mutants with altered glycyl transfer ribonucleic acid synthetases. J Bacteriol (1970) 2.07
Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol Cell Biol (1988) 2.06
A single mutational modification of a tryptophan-specific transfer RNA permits aminoacylation by glutamine and translation of the codon UAG. J Mol Biol (1974) 2.05
Simian virus 40 mutants with deletions at the 3' end of the early region are defective in adenovirus helper function. J Virol (1979) 2.01
Asilomar conference on recombinant DNA molecules. Science (1975) 2.00
Studies of missense suppression of the tryptophan synthetase A-protein mutant A36. Proc Natl Acad Sci U S A (1966) 1.97
An adenovirus E1A-like transcription factor is regulated during the differentiation of murine embryonal carcinoma stem cells. Cell (1987) 1.96
Transcripts regulated during normal embryonic development and oncogenic transformation share a repetitive element. Cell (1983) 1.94
A role for the TATA-box-binding protein component of the transcription factor IID complex as a general RNA polymerase III transcription factor. Proc Natl Acad Sci U S A (1992) 1.85
A mammalian protein complex that repairs double-strand breaks and deletions by recombination. J Biol Chem (1993) 1.85
Purification and physical characterization of tyrosyl ribonucleic acid synthetases from Escherichia coli and Bacillus subtilis. Biochemistry (1966) 1.83
A fragment of the SV40 large T-antigen gene transforms. Nature (1982) 1.82
Regulation of RNA polymerase III transcription in response to F9 embryonal carcinoma stem cell differentiation. Cell (1989) 1.81
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