Targeting of signal sequenceless proteins for export in Escherichia coli with altered protein translocase.

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Published in EMBO J on October 01, 1996

Authors

W A Prinz1, C Spiess, M Ehrmann, C Schierle, J Beckwith

Author Affiliations

1: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.

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Use of gene fusions to determine the orientation of gene phoA on the Escherichia coli chromosome. J Bacteriol (1981) 1.74

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Bridge over troubled waters: sensing stress by disulfide bond formation. Cell (1999) 1.71

Cyclic adenosine monophosphate-independent mutants of the lactose operon of Escherichia coli. J Bacteriol (1973) 1.68

Use of gene fusions to isolate promoter mutants in the transfer RNA gene tyrT of Escherichia coli. J Mol Biol (1979) 1.68